Transcription regulatory networks underlying specification of neuronal types in the nervous system of the ascidian

• Project/Area Number: 18370089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: KUSAKABE Takehiro University of Hyogo, 大学院・生命理学研究科, 准教授 (40280862)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,320,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥3,120,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
• Keywords: ゲノム / 遺伝子 / 発生・分化 / 発現制御 / 脳・神経

Research Abstract

体のつくりや遺伝子構成が単純な海産動物ホヤをモデルとして、神経細胞の個性がつくられる仕組みを探った。グルタミン酸、GABAなど主要な神経伝達物質を使う神経細胞について、各細胞に固有の性質を与える遺伝子が特異的にはたらく仕組みの一端を解明した。ゲノム情報を生物間で比較することによって、遺伝情報の読み取りを調節するDNA配列(シス調節配列)とそこに結合するタンパク質(転写調節因子)を同定した。また、様々な神経細胞ではたらくシス調節配列を解析し、結果をデータベースとして公開した。

Research Products

• [Journal Article] Simple motor system of the ascidian larva : neuronal complex comprising putative cholinergic and GABAergic/glycinergic neurons (2010)
  • Author(s): Takeo Horie, Masashi Nakagawa, Yasunori Sasakura, Takehiro G. Kusakabe, Motoyuki Tsuda
  • Journal Title: Zool.Sci. 27(2) Pages: 181-190
• [Journal Article] Distinctive expression patterns of Hedgehog pathway genes in the Ciona intestinalis larva : implications for a role of Hedgehog signaling in postembryonic development and chordate evolution (2010)
  • Author(s): Tariq A. F. M. Tariqul Islam, Pricila Khan Moly, Yuki Miyamoto, Takehiro G. Kusakabe
  • Journal Title: Zool.Sci. 27(2) Pages: 84-90
• [Journal Article] Evolution and the origin of the visual retinoid cycle in vertebrates (2009)
  • Author(s): Takehiro G. Kusakabe, Noriko Takimoto, Minghao Jin, Motoyuki Tsuda
  • Journal Title: Phil.Trans.R.Soc.Lond. 364(1531) Pages: 2897-2910
• [Journal Article] Tube formation by complex cellular processes in Ciona intestinalis notochord (2009)
  • Author(s): Bo Dong, Takeo Horie, Elsa Denker, Takehiro G. Kusakabe, Motoyuki Tsuda, William C. Smith, Di Jiang
  • Journal Title: Dev.Biol. 330(2) Pages: 237-249
• [Journal Article] Cell type and function of neurons in the ascidian nervous system (2009)
  • Author(s): Takeo Horie, Yasunori Sasakura, Masashi Nakagawa, Takehiro G. Kusakabe
  • Journal Title: Dev.Growth & Differ 51(3) Pages: 207-220
• [Journal Article] Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva. (2009)
  • Author(s): Takeo Horie, Daisuke Sakurai, Hisashi Ohtsuki, Akihisa Terakita, Yoshinori Shichida, Jiro Usukura, Takehiro Kusakabe, Motoyuki Tsuda
  • Journal Title: J.Comp.Neurol. 509(1) Pages: 88-102
• [Journal Article] Cell type and function of neurons in the ascidian nervoussystem (2009)
  • Author(s): Takeo Horie
  • Journal Title: Development, Growth & Differentiation 51 Pages: 207-220
  • Peer Reviewed
• [Journal Article] Tube formation by complex cellular processes in Ciona intestinalis notochord (2009)
  • Author(s): Bo Dong
  • Journal Title: Developmental Biology (印刷中)
  • Peer Reviewed
• [Journal Article] Markov chain-based promoter structure modeling for tissue-specific expression pattern prediction (2008)
  • Author(s): Alexis Vandenbon, Yuki Miyamoto, Noriko Takimoto, Takehiro Kusakabe, Kenta Nakai
  • Journal Title: DNA Res. 15(1) Pages: 3-11
• [Journal Article] Glutamatergic networks in the Ciona intestinalis larva (2008)
  • Author(s): Takeo Horie, Takehiro Kusakabe, Motoyuki Tsuda
  • Journal Title: J.Comp.Neurol. 508(2) Pages: 249-263
• [Journal Article] Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva (2008)
  • Author(s): Takeo Horie
  • Journal Title: Journal of Comparative Neurology 509 Pages: 88-102
  • Peer Reviewed
• [Journal Article] Glutamatergic networks in the Ciona intestinalis larva (2008)
  • Author(s): Takeo Horie
  • Journal Title: Journal of Comparative Neurology 508 Pages: 249-263
  • Peer Reviewed
• [Journal Article] Markov chain-based promoter struture modeling for tissue-specific expression pattern prediction (2008)
  • Author(s): Alexis Vandenbon
  • Journal Title: DNA Research 15 Pages: 3-11
  • Peer Reviewed
• [Journal Article] Glutamatergic networks in the Ciona intestinalis larva (2008)
  • Author(s): Takeo Horie
  • Journal Title: Journal of Comparative Neurology 509 Pages: 88-102
  • Peer Reviewed
• [Journal Article] Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in (2007)
  • Author(s): Reiko Yoshida, Takeo Horie, Motoyuki Tsuda, Takehiro G. Kusakabe
  • Journal Title: Ciona intestinalis larva.Dev.Growth Differ. 49(8) Pages: 657-667
• [Journal Article] Photoreceptive systems in ascidians (2007)
  • Author(s): Takehiro Kusakabe, Motoyuki Tsuda
  • Journal Title: Photochem.Photobiol. 83(2) Pages: 248-252
• [Journal Article] Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in Ciona intestinalis larva (2007)
  • Author(s): Reiko Yoshida
  • Journal Title: Development, Growth & Differentiation 49 Pages: 657-667
  • Peer Reviewed
• [Journal Article] photoreceptive systems in ascidians (2007)
  • Author(s): Takehiro Kusakabe
  • Journal Title: Photochemistry and Photobiology 83 Pages: 248-252
• [Presentation] Decoding cis-regulatory systems of cell type-specific genes in the tunicate genome (2009)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: International Symposium on Marine Genomics 2009
  • Place of Presentation: Southern Plaza Kaiho, Okinawa
  • Year and Date: 2009-12-18
• [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性ニューロンにおける遺伝子発現を制御するシス調節配列と転写調節因子の機能解析 (2009)
  • Author(s): 西辻光希
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] ホヤ幼生におけるグルタミン酸作動性ニューロン特異的な遺伝子発現調節機構とPOU転写遺伝子Ci-P0U-IVの役割 (2009)
  • Author(s): 大道裕
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] cis-Regulatory modules and transcription factors that control gene expression in glutamatergic and GABAergic/glycinergic neurons in the Ciona intestinalis larva (2009)
  • Author(s): Koki Nishitsuji
  • Organizer: 5th Tunicate Meeting
  • Place of Presentation: Okinawa Industry Support Center
  • Year and Date: 2009-06-24
• [Presentation] Dopaminergic cells in Ciona intestinalis larva share some features with vertebrate dopaminergic amacrine cells. (2009)
  • Author(s): Florian Razy-Krajka
  • Organizer: 5th Tunicate Meeting
  • Place of Presentation: Okinawa Industry Support Center
  • Year and Date: 2009-06-24
• [Presentation] Ptfl, a bHLH transcription factor, defines the dopaminergic neuronal cell fate in ascidian larva (2009)
  • Author(s): Takeo Horie
  • Organizer: 5th Tunicate Meeting
  • Place of Presentation: Okinawa Industry Support Center
  • Year and Date: 2009-06-24
• [Presentation] Distinct expression patterns of hedgehog signaling pathway genes during larval development of Ciona intestinalis (2009)
  • Author(s): A. F. M. Tariqul Islam
  • Organizer: 5th Tunicate Meeting
  • Place of Presentation: Okinawa Industry Support Center
  • Year and Date: 2009-06-21
• [Presentation] Neuronal differentiation in the central nervous system territories marked by the expression of Otx, Pax-2/5/8, and Engenes in the ascidian larva (2009)
  • Author(s): Koki Nishitsuji
  • Organizer: 日本発生生物学会第42回大会
  • Place of Presentation: 朱鷺メッセ 新潟コンべンシヨンセンター
• [Presentation] DiI標識とレーザー細胞破壊によるカタユウレイボヤ幼生の光受容細胞の細胞系譜解析 (2008)
  • Author(s): A. F. M. Tariqul Islam
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] カタユウレイボヤ神経系特異的遺伝子の転写調節領域の大規模解析 (2008)
  • Author(s): 西辻光希
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] ホヤ幼生の神経発生におけるOtxとPax2/5/8の役割 (2008)
  • Author(s): 日下部岳広
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-09-07
• [Presentation] Cell lineage analysis of photoreceptor cells by laser ablation and DiI labeling in the Ciona intestinalis larva (2008)
  • Author(s): A. F. M. Tariqul Islam
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] ホヤにおける視覚サイクル機構 (2008)
  • Author(s): 瀧本 紀子
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] Identification and characterization of a cis-regulatory module required for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva (2008)
  • Author(s): Koki Nishitsuji
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Ontogeny of glutamatergic, GABA/glycinergic, and cholinergic neurons in the Ciona intestinalis larva (2008)
  • Author(s): Yuki Morimoto
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Ci-POU-IV, a POU domain transcription factor, is required for development of epidermal glutamatergic neurons in the Ciona intestinalis larva (2008)
  • Author(s): Takako Suzuki
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Roles of hedgehog signaling in Ciona intestinalis development : insights from hedgehog and Gli expression and cyclopamine treatment (2008)
  • Author(s): A. F. M. Tariqul Islam
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Identification and characterization of a cis-regulatory module required for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva. (2008)
  • Author(s): Koki Nishitsuji
  • Organizer: Joint meeting of the Societe Franqaise de Biologic du Development / Japanese Society of Developmental Biologists
  • Place of Presentation: 日 GIENS (FRANCE)
• [Presentation] Gene regulatory mechanisms underlying the determination of neuronal identity in the ascidian larva. (2008)
  • Author(s): Takehiro Kusakabe
  • Organizer: The 16th CDB Meeting : Cis-sequence Regulation and its Evolution
  • Place of Presentation: 理研 CDB (神戸)
• [Presentation] Roles of hedgehog signaling in Ciona intestinalis development : insights from hedgehog and Gli expression and cyclopamine treatment (2008)
  • Author(s): A. F. M. Tariqul Islam
  • Organizer: 日本発生生物学会第4 1回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Ontogeny of glutamatergic, GABA/glycinergic, and cholinergicneurons in the Ciona intestinalis larva (2008)
  • Author(s): 森本 熊紀
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Ci-POU-IV, a POU domain transcription factor, is required for development of epidermal glutamatergic neurons in the Cionaintestinalis larva (2008)
  • Author(s): 鈴木 貴子
  • Organizer: 日本発生生物学会第41回大会
  • Place of Presentation: 徳島県郷土文化会館
• [Presentation] Identification and characterization of a cis-regulatory modulerequired for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva (2008)
  • Author(s): Koki Nishitsuji
  • Organizer: Joint meeting of the Societe Francaisede Biologie du Development / Japanese Society of Developmental Biologists
  • Place of Presentation: GIENS (FRANCE)
• [Presentation] Gene Regulatory Mechanisms underlying the Determination of Neuronal Identity in the Ascidian Larva (2008)
  • Author(s): Takehiro Kusakabe
  • Organizer: The 16th CDB Meeting : Cis-sequence Regulation and its Evolution
  • Place of Presentation: 理研CDB(神戸)
• [Presentation] Molecular and cellular mechanisms of glutamatergic and GABAergic neuron development in the ascidian larva (2007)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: The Sixth Okazaki Biology Conference "Marine Biology"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2007-12-05
• [Presentation] Molecular and celluar mechanisms of glutamatergic and GAB Aergicneuron development in the ascidian larva (2007)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: The SiXth Okazaki Biology Conference
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-12-05
• [Presentation] ホヤの光受容システムから探る脊椎動物の頭部感覚器官の起源 (2007)
  • Author(s): 日下部 岳広
  • Organizer: 日本進化学会第9回京都大会
  • Place of Presentation: 京都大学 吉田キャンパス
  • Year and Date: 2007-08-31
• [Presentation] 比較ゲノムによるカタユウレイボヤ GABA 作動性ニューロンおよびグルタミン酸作動性ニューロンのシス調節領域の同定 (2007)
  • Author(s): 西辻光希
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] 比較ゲノムによるカタユウレイボヤGABA作動性ニューロンおよびグルタミン作動性ニューロンのシス調節領域の同定 (2007)
  • Author(s): 西辻 光希
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] カタユウレイボヤ胚発生および変態過程における光受容器官の形成機構と脊椎動物の眼および松果体の起源 (2007)
  • Author(s): 笹倉 美幸
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] 転写制御領域に見られる共通構造のコンピューターモデリングに向けて (2007)
  • Author(s): 中井謙太
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会 合同大会 ミニシンポジウム「形を作る遺伝子調節ネットワーク」
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-28
• [Presentation] 転写制御領域に見られる共通構造のコンピューターモデリングに向けて (2007)
  • Author(s): 中井 謙太
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-28
• [Presentation] カタユウレイボヤ幼生のコリン作動性およびGABA作動性神経回路の可視化 (2007)
  • Author(s): 森元熊紀
  • Organizer: 2007年度日本動物学会近畿支部研究発表会
  • Place of Presentation: 神戸山手大学
  • Year and Date: 2007-05-19
• [Presentation] 比較ゲノムによるニューロン特異的遺伝子のシス調節モジュールの同定 (2007)
  • Author(s): 西辻光希
  • Organizer: 2007年度日本動物学会近畿支部研究発表会
  • Place of Presentation: 神戸山手大学
  • Year and Date: 2007-05-19
• [Presentation] Role of hedgehog signaling in neuronal patterning of the Ciona intestinalis larva (2007)
  • Author(s): A. E M. Tariqul Islam
  • Organizer: 2007年度日本動物学会近畿支部研究発表会
  • Place of Presentation: 神戸山手大学
  • Year and Date: 2007-05-19
• [Presentation] GFP/WGA二重標識によるホヤ幼生のコリン作動性およびGABA作動性神経回路の可視化 (2007)
  • Author(s): 森元熊紀
  • Organizer: 第2回ホヤ研究集会
  • Place of Presentation: 下田
  • Year and Date: 2007-05-08
• [Presentation] Markov chain-based promoter structure modeling (2007)
  • Author(s): Alexis Vandenbon
  • Organizer: 4th International Tunicate Meeting
  • Place of Presentation: Villefranche-sur-Mer.
• [Presentation] Markov chain-based promoter structure modeling. (2007)
  • Author(s): Alexis Vandenbon
  • Organizer: 15th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB) & 6th European Conference on Computational Biology (ECCB)
  • Place of Presentation: Vienna, Austria
• [Book] 『動物の多様な生き方5さまざまな神経系をもつ動物たち : 神経系の比較生物学』(比較生理生化学会編) (2009)
  • Author(s): 日下部岳広 (共著)
  • Publisher: 共立出版
• [Book] 『身近な動物を使った実験1-ホヤ メダカ・ゼブラフィッシュ キンギョカエル』(鈴木範男編) (2009)
  • Author(s): 日下部岳広 (共著)
  • Publisher: 三共出版
• [Book] 身近な動物を使った実験1 ホヤ (2009)
  • Author(s): 日下部 岳広
  • Publisher: 三共出版
• [Book] 動物はなぜ多様な神経系をもっか? : 神経系の比較生物学第10章 ホヤの神経系と行動 (2009)
  • Author(s): 日下部 岳広
  • Publisher: 共立出版(印刷中)
• [Remarks] ホヤ遺伝子制御データベース (DBTGR)
  • URL: http://dbtgr.hgc.jp/
• [Remarks] ホヤ統合データベース (Aniseed)
  • URL: http://crfb.univ-mrs.fr/aniseed/