Mechanism of light sensing and signaling mediated by phytochrome and its interacting proteins in plants
Project/Area Number |
18380200
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Kyoto University |
Principal Investigator |
KOHCHI Takayuki Kyoto University, 大学院・生命科学研究科, 教授 (40202056)
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Co-Investigator(Kenkyū-buntansha) |
大和 勝幸 京都大学, 生命科学研究科, 助教 (50293915)
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Co-Investigator(Renkei-kenkyūsha) |
YAMATO T.Katsuyuki 京都大学, 大学院・生命科学研究科, 助教 (50293915)
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Project Period (FY) |
2006 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥17,250,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
|
Keywords | 発生・分化制御 / タンパク質間相互作用 / 植物光信号伝達 / 光受容体 / フィトクロム / 環境応答 / シロイヌナズナ / タンパク質相互作用 / 光信号伝達 / 光背センシング / 光形態形成 / 光受容 / 遺伝子発現制御 / 信号伝達 / 遺伝子発現 |
Research Abstract |
The novel zinc finger proteins were identified by protein interaction screening with phytochromes. The mutants having T-DNA insertion in the corresponding genes showed late flowering phenotype. The genetic analysis indicated the genes regulated FT expression as downstream factors of phytochrome B. The studies of temporal and spatial expression, phenotypic complementation with the modified genes indicated the proteins functioned in the nucleus in vascular tissue. Our finding gives insight on the link between light environment and development in growth control.
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Report
(6 results)
Research Products
(44 results)
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[Journal Article] Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells2007
Author(s)
Chiyoda, S., Linley, P. J., Yamato, K. T., Fukuzawa, H., Yokota, A., and Kohchi, T.
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Journal Title
Transgenic Res 16
Pages: 41-49
Related Report
Peer Reviewed
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[Journal Article] Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells.2007
Author(s)
Chiyoda, S., Linley, P.J., Yamato, K.T., Fukuzawa, H., Yokota, A., Kohchi, T.
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Journal Title
Transgenic Res. 16
Pages: 41-49
Related Report
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[Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.2006
Author(s)
Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., Kohchi, T.
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Journal Title
FEBS Lett. 580
Pages: 1333-1338
Related Report
Peer Reviewed
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[Journal Article] Reconstitution of blue/green reversible photoconversion of a cyanobacterial photoreceptor. PixJ1 in phycocyanobilin producing E. coli.2006
Author(s)
Yoshihara, S., Shimada, T., Matsuoka, D., Zikihara, K., Kohchi, T., Tokutomi, S.
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Journal Title
Biochem. 45
Pages: 3775-3784
Related Report
Peer Reviewed
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[Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli2006
Author(s)
Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., and Kohchi, T.
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Journal Title
FEBS Lett 580
Pages: 1333-1338
Related Report
Peer Reviewed
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[Journal Article] Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli2006
Author(s)
Mukougawa, K., Kanamoto, H., Kobayashi, T., Yokota, A., Kohchi, T.
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Journal Title
FEBS Lett. 580
Pages: 1333-1338
Related Report
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