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Potential of producing value-added chemical products using waste activated sludge as the catalyst in municipal wastewater treatment plants

Research Project

Project/Area Number 18H01570
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 22060:Environmental systems for civil engineering-related
Research InstitutionOsaka University

Principal Investigator

Ike Michihiko  大阪大学, 工学研究科, 教授 (40222856)

Co-Investigator(Kenkyū-buntansha) 井上 大介  大阪大学, 工学研究科, 准教授 (70448091)
黒田 真史  大阪大学, 工学研究科, 助教 (20511786)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords下水処理場 / 余剰汚泥 / 産業廃水 / 化成品生産 / バイオマス循環拠点化 / 産業排水
Outline of Final Research Achievements

This project aimed to the realization of the production of value-added products using waste activated sludge (WAS) as the catalyst in municipal wastewater treatment plants, and conducted fundamental studies to the aim. First, we evaluated the potential of WAS to produce polyhydroxyalkanoates (PHA), glycogen (GLG), and triacylglycerol (TAG) from various substrates. Then, focusing on PHA, we established the method to efficiently enrich PHA-accumulating bacteria in WAS within short periods. In addition, we established the method to efficiently recover PHA accumulated in WAS. Finally, we estimated the environmental burdens during the PHA production using WAS, and confirmed the usefulness of the proposed PHA production strategy.

Academic Significance and Societal Importance of the Research Achievements

下水処理場は、従来、産業の静脈側の役割を担ってきたが、本研究の構想が実現すれば、モノ創りという動脈側の拠点の役割も付与することができ、循環型産業システムの構築に大いに貢献する可能性を有する。本研究では、余剰汚泥を用いた特定の化成品合成として、PHA、GLG、TAGの合成可能性について検討し、特にPHAに着目して、余剰汚泥のPHA生産機能を向上させる手法及び汚泥からPHAを効率的に回収する手法を確立し、提案構想を実現していくための重要な基礎を築くことができ、学術的にも社会的にも意義の高い成果が得られたものといえる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (22 results)

All 2022 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (19 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results)

  • [Journal Article] Optimization of aerobic dynamic discharge process for very rapid enrichment of polyhydroxyalkanoates-accumulating bacteria from activated sludge2021

    • Author(s)
      Daisuke Inoue, Atsushi Fukuyama, Yu Ren, Michihiko Ike
    • Journal Title

      Bioresource Technology

      Volume: 336 Pages: 125314-125314

    • DOI

      10.1016/j.biortech.2021.125314

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid enrichment of polyhydroxyalkanoate-accumulating bacteria by the aerobic dynamic discharge process: Enrichment effectiveness, polyhydroxyalkanoate accumulation ability, and bacterial community characteristics in comparison with the aerobic dynamic feeding process2019

    • Author(s)
      Inoue Daisuke、Fukuyama Atsushi、Ren Yu、Ike Michihiko
    • Journal Title

      Bioresource Technology Reports

      Volume: 7 Pages: 100276-100276

    • DOI

      10.1016/j.biteb.2019.100276

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential of waste activated sludge to accumulate polyhydroxyalkanoates and glycogen using industrial wastewater/liquid wastes as substrates2019

    • Author(s)
      Ike Michihiko、Okada Yukihiro、Narui Takaaki、Sakai Kosuke、Kuroda Masashi、Soda Satoshi、Inoue Daisuke
    • Journal Title

      Water Science and Technology

      Volume: 80 Issue: 12 Pages: 2373-2380

    • DOI

      10.2166/wst.2020.059

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] グルコースを基質とした余剰汚泥からのポリヒドロキシアルカン酸蓄積微生物の迅速集積法の確立2022

    • Author(s)
      辻柾哉、任羽、井上大介、池道彦
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Substrate versatility for polyhydroxyalkanoates production by acetate-grown mixed microbial culture derived from activated sludge2021

    • Author(s)
      Yu Ren, Daisuke Inoue, Michihiko Ike
    • Organizer
      Water and Environment Technology Conference 2021 (WET2021)-online
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Enrichment of Polyhydroxyalkanoates-accumulating bacteria from activated sludge using glucose as substrate2021

    • Author(s)
      Masaya Tsuji, Yu Ren, Daisuke Inoue, Michihiko Ike
    • Organizer
      The 13th Joint Workshop on Advanced Engineering Technology for Environment and Energy
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 余剰汚泥に蓄積したポリヒドロキシアルカン酸の高効率回収法の検討2021

    • Author(s)
      井上大介、三和康平、池道彦
    • Organizer
      第58回下水道研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 余剰汚泥に蓄積したポリヒドロキシアルカン酸の高効率回収法の確立2021

    • Author(s)
      三和康平, 井上大介, 池道彦
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Optimization of operational variables for aerobic dynamic discharge process to efficiently enrich polyhydroxyalkanoate-accumulating bacteria in activated sludge2020

    • Author(s)
      Daisuke Inoue, Atsushi Fukuyama, Yu Ren, Michihiko Ike
    • Organizer
      Sustainable Waste Management Conference 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 余剰汚泥に蓄積したポリヒドロキシアルカン酸の高収率・高純度な回収法の検討2020

    • Author(s)
      井上大介, 三和康平, 池道彦
    • Organizer
      第57回環境工学研究フォーラム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 余剰汚泥に蓄積したポリヒドロキシアルカン酸の高効率回収法の確立2020

    • Author(s)
      三和康平、井上大介、池道彦
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 余剰汚泥からのポリヒドロキシアルカン酸蓄積微生物の迅速集積技術の確立2020

    • Author(s)
      任羽、福山篤史、井上大介、池道彦
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 油糧微生物のバイオオイル生産特性に関する検討2019

    • Author(s)
      三和康平、黒田真史、井上大介、池道彦
    • Organizer
      第19回環境技術学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 余剰汚泥からのポリヒドロキシアルカン酸の迅速集積法の検討2019

    • Author(s)
      井上大介、福山篤史、任羽、池道彦
    • Organizer
      第56回下水道研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Potential of waste activated sludge to accumulate polyhydroxyalkanoates and glycogen using industrial wastewater/liquid wastes as substrates2019

    • Author(s)
      Michihiko Ike, Yukihiro Okada, Takaaki Narui, Kosuke Sakai, Masashi Kuroda, Satoshi Soda, Daisuke Inoue
    • Organizer
      10th IWA International Symposium on Waste management Problems in Agro-Industries
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid enrichment of polyhydroxyalkanoates-accumulating bacteria from activated sludge by aerobic dynamic discharge process2019

    • Author(s)
      Atsushi Fukuyama, Yu Ren, Daisuke Inoue, Michihiko Ike
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optimization of operational conditions of aerobic dynamic discharge process for rapid enrichment of polyhydroxyalkanoates-accumulating bacteria from activated sludge2019

    • Author(s)
      Atsushi Fukuyama, Yu Ren, Daisuke Inoue, Michihiko Ike
    • Organizer
      the 12th Joint Workshop on Advanced Engineering Technology for Environment and Energy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 余剰汚泥を活用した下排水からのバイオプラスチック生産への挑戦2019

    • Author(s)
      井上大介
    • Organizer
      日本生物工学会北日本支部2019札幌シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 余剰汚泥からのPHA蓄積微生物の迅速集積法の確立~下水処理で発生する廃棄物の高付加価値資源への転換への挑戦~2019

    • Author(s)
      井上大介
    • Organizer
      日本水処理生物学会第56回大会 排水・環境水研究集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Accumulation of intracellular storage compounds by waste activated sludge as value-added products: for turning wastewater treatment plants to biorefinery plants2019

    • Author(s)
      Michihiko Ike, Daisuke Inoue
    • Organizer
      IEEC & BWR2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 余剰汚泥中のポリヒドロキシアルカン酸蓄積微生物の迅速集積に関する基礎的検討2019

    • Author(s)
      井上大介、福山篤史、池道彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Potential of PHA and glycogen accumulation from industrial wastewater in waste activated sludge2018

    • Author(s)
      Daisuke Inoue, Takaaki Narui, Yukihiro Okada, Kazunori Yokoe, Masashi Kuroda, Satoshi Soda, Michihiko Ike
    • Organizer
      Grand Renewable Energy 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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