Single-molecule analysis by nanoporescopy

• Project/Area Number: 18H01846
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28050:Nano/micro-systems-related
• Research Institution: Osaka University
• Principal Investigator: Tsutsui Makusu 大阪大学, 産業科学研究所, 准教授 (50546596)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: ナノポア / イオン電流 / 1分子泳動ダイナミクス / 1分子解析 / タンパク質 / 電気浸透流 / ナノ流体 / 1分子ダイナミクス / ナノ科学 / 輸送現象 / センサ / MEMS / マイクロ・ナノデバイス / バイオセンサ / 界面

Outline of Final Research Achievements

Low thickness-to-diameter aspect ratio nanopores were fabricated for structure analyses of individual particles and molecules in electrolyte solution. The nanosensor consisted of a nanoscale hole formed in an ultra-thin membrane. A gate electrode was also embedded around the pore that enabled to slow down the translocation motions of the objects via the gating-induced electroosmotic flow. Overall capability allowed to discriminate various substances from bacterial cells to proteins via the ionic current signal waveforms.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した液中1粒子・分子形状解析法は、物質の体積だけが測定可能な従来法とは異なり、物質の表面電荷や質量、表面タンパクの種類など、様々な性状の違いを見分けることができる手法であり、また、ウイルスやタンパク質、DNAなどあらゆる生体分子に応用可能な検出原理を備えたセンサ技術である。当該技術がタンパク質構造解析やDNA塩基配列解読に広く活用されることで、構造生物学、ひいては生命科学の発展に大きく寄与することができると期待される。

Research Products

• [Journal Article] Field effect control of translocation dynamics in surround-gate nanopores (2021)
  • Author(s): Makusu Tsutsui, Sou Ryuzaki, Kazumichi Yokota, Yuhui He, Takashi Washio, Kaoru Tamada, and Tomoji Kawai
  • Journal Title: Communications Materials Volume: 2 Issue: 1 Pages: 29-29
  • DOI: 10.1038/s43246-021-00132-3
  • Peer Reviewed
• [Journal Article] Nano-corrugated Nanochannels for In Situ Tracking of Single-Nanoparticle Translocation Dynamics (2020)
  • Author(s): Tsutsui Makusu、Yokota Kazumichi、He Yuhui、Washio Takashi、Kawai Tomoji
  • Journal Title: ACS Sensors Volume: 5 Issue: 8 Pages: 2530-2536
  • DOI: 10.1021/acssensors.0c00845
  • Peer Reviewed
• [Journal Article] Electroosmosis-Driven Nanofluidic Diodes (2020)
  • Author(s): Leong Iat Wai、Tsutsui Makusu、Murayama Sanae、He Yuhui、Taniguchi Masateru
  • Journal Title: The Journal of Physical Chemistry B Volume: 124 Issue: 32 Pages: 7086-7092
  • DOI: 10.1021/acs.jpcb.0c04677
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Solid-State Nanopore Time-of-Flight Mass Spectrometer (2019)
  • Author(s): Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Yuhui He, Tomoji Kawai
  • Journal Title: ACS Sensors Volume: 4 Issue: 11 Pages: 2974-2979
  • DOI: 10.1021/acssensors.9b01470
  • Peer Reviewed
• [Journal Article] Tailoring Dielectric Surface Charge via Atomic Layer Thickness (2019)
  • Author(s): Hayashida Tomoki、Yokota Kazumichi、Murayama Sanae、Arima Akihide、Tsutsui Makusu、Taniguchi Masateru
  • Journal Title: ACS Applied Materials & Interfaces Volume: 12 Issue: 4 Pages: 5025-5030
  • DOI: 10.1021/acsami.9b18444
  • Peer Reviewed / Open Access
• [Journal Article] Back-Side Polymer-Coated Solid-State Nanopore Sensors (2019)
  • Author(s): Leong Iat Wai、Tsutsui Makusu、Nakada Tomoko、Taniguchi Masateru、Washio Takashi、Kawai Tomoji
  • Journal Title: ACS Omega Volume: 4 Issue: 7 Pages: 12561-12566
  • DOI: 10.1021/acsomega.9b00946
  • Peer Reviewed / Open Access
• [Journal Article] Heat dissipation in quasi-ballistic single-atom contacts at room temperature (2019)
  • Author(s): Makusu Tsutsui, Yu-Chang Chen
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 18677-18677
  • DOI: 10.1038/s41598-019-55048-3
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput single nanoparticle detection using a feed-through channel-integrated nanopore (2019)
  • Author(s): Makusu Tsutsui, Tomoko Yamazaki, Kenji Tatematsu, Kazumichi Yokota, Yuko Esaki, Yukari Kubo, Hiroko Deguchi, Akihide Arima, Shun'ichi Kuroda, Tomoji Kawai
  • Journal Title: Nanoscale Volume: 11 Issue: 43 Pages: 20475-20484
  • DOI: 10.1039/c9nr07039g
  • Peer Reviewed
• [Journal Article] Electric field interference and bimodal particle translocation in nano-integrated multipores (2019)
  • Author(s): Makusu Tsutsui, Kazumichi Yokota, Tomoko Nakada, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, and Tomoji Kawai
  • Journal Title: Nanoscale Volume: 11 Issue: 16 Pages: 7547-7553
  • DOI: 10.1039/c8nr08632j
  • Peer Reviewed / Open Access
• [Journal Article] Silicon substrate effects on ionic current blockade in solid-state nanopores (2019)
  • Author(s): Makusu Tsutsui, Kazumichi Yokota, Tomoko Nakada, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, and Tomoji Kawai
  • Journal Title: Nanoscale Volume: 11 Issue: 10 Pages: 4190-4197
  • DOI: 10.1039/c8nr09042d
  • Peer Reviewed / Open Access
• [Journal Article] Identifying single viruses using biorecognition solid-state nanopores (2018)
  • Author(s): Akihide Arima, Ilva Hanun Harlisa, Takeshi Yoshida, Makusu Tsutsui, Masayoshi Tanaka, Kazumichi Yokota, Wataru Tonomura, Jiro Yasuda, Masateru Taniguchi, Takashi Washio, Mina Okochi, and Tomoji Kawai
  • Journal Title: journal of American Chemical Society Volume: 140 Issue: 48 Pages: 16834-16841
  • DOI: 10.1021/jacs.8b10854
  • Peer Reviewed
• [Journal Article] Particle capture in solid-state multipores (2018)
  • Author(s): Makusu Tsutsui, Kazumichi Yokota, Tomoko Nakada, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, and Tomoji Kawai
  • Journal Title: ACS Sensors Volume: 3 Issue: 12 Pages: 2693-2671
  • DOI: 10.1021/acssensors.8b01214
  • Peer Reviewed / Open Access
• [Journal Article] Temporal response of ionic current blockade in solid-state nanopores (2018)
  • Author(s): Makusu Tsutsui, Kazumichi Yokota, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, and Tomoji Kawai
  • Journal Title: ACS PApplied Materials & Interfaces Volume: 10 Issue: 40 Pages: 34751-34751
  • DOI: 10.1021/acsami.8b11819
  • Peer Reviewed
• [Journal Article] Impact of ionization equilibrium on electrokinetic flow of weak electrolytes in nanochannels (2018)
  • Author(s): Ziwei Ji, Zhuo Huang, Bowei Chen, Yuhui He, Makusu Tsutsui, Xiangshui Miao
  • Journal Title: Nanotechnology Volume: 29 Issue: 29 Pages: 295402-295402
  • DOI: 10.1088/1361-6528/aac126
  • Peer Reviewed
• [Presentation] ナノポアと機械学習を用いたウイルス検査 (2021)
  • Author(s): 筒井真楠
  • Organizer: 応用物理学会
  • Invited
• [Presentation] 低アスペクト比ナノポアを用いた1粒子質量測定 (2020)
  • Author(s): 筒井真楠，横田一道，有馬彰秀，ハ―ユフイ, 川合知二
  • Organizer: 応用物理学会
• [Presentation] マルチナノポアを用いた高効率1粒子検出 (2019)
  • Author(s): 筒井真楠、横田一道、中田知子、有馬彰秀、殿村渉、谷口正輝、鷲尾隆、川合知二
  • Organizer: 応用物理学会
• [Presentation] Single-bacteria and virus identification using a bio-recognition nanopore (2019)
  • Author(s): Makusu Tsutsui, Akihide Arima, Ilva Hanun Harlisa, Takeshi Yoshida, Masayoshi Tanaka, Kazumichi Yokota, Wataru Tonomura, Jiro Yasuda, Masateru Taniguchi, Takashi Washio, Mina Okochi, Tomoji Kawai
  • Organizer: Materials Research Society, 2019 Fall Meeting
  • Int'l Joint Research
• [Presentation] Virus infection diagnosis by AI-driven nanopore sensing (2019)
  • Author(s): Makusu Tsutsui
  • Organizer: Sixteenth International Conference on Flow Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Al2O3成膜によるマイクロポア壁面の表面電荷の調整 (2019)
  • Author(s): 林田 朋樹、筒井 真楠、谷口 正輝
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] ナノポアトラップ法による単一粒子の捕捉と識別 (2019)
  • Author(s): 有馬 彰秀、筒井 真楠、谷口 正輝
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] ナノポアによるメソポーラス・ナノ粒子の計測 (2019)
  • Author(s): 岸本 匠平、筒井 真楠、谷口 正輝
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] 機械学習と分子認識ナノポアを用いた1ウイルス識別 (2019)
  • Author(s): 筒井 真楠、有馬 彰秀、ハルリサ イルファ、吉田 剛、田中 祐圭、横田 一道、殿村 渉、鷲尾 隆、大河内 美奈、川合 知二
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] ナノポア計測による多項目ウイルス識別 (2019)
  • Author(s): 有馬 彰秀、筒井 真楠、殿村 渉、横田 一道、立松 健司、山﨑 智子、黒田 俊一、谷口 正輝、鷲尾 隆、川合 知二
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] Characterization of single nanoparticle shape using solid state nanopore (2019)
  • Author(s): Iatwai Leong、Makusu TSUTSUI、Masateru TANIGUCHI
  • Organizer: 第66回応用物理学会春季学術講演会