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Exploring the target pathways of key regulators for dormancy and chilling requirement in Rosaceae fruit trees

Research Project

Project/Area Number 18H02198
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionKyoto University

Principal Investigator

Yamane Hisayo  京都大学, 農学研究科, 准教授 (80335306)

Co-Investigator(Kenkyū-buntansha) 玉田 洋介  宇都宮大学, 工学部, 准教授 (50579290)
富永 晃好  静岡大学, 農学部, 助教 (50776490)
羽生 剛  愛媛大学, 農学研究科, 准教授 (60335304)
高居 恵愛  石川県立大学, 生物資源環境学部, 准教授 (70589770)
池田 和生  山形大学, 農学部, 准教授 (80555269)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords果樹 / 休眠 / 温暖化 / 転写因子 / ホルモン / エピジェネティック / ヒストン修飾 / 低温要求性 / エピジェネティクス / MADS-box
Outline of Final Research Achievements

The objective of this study was to elucidate the functions and downstream regulatory mechanisms of DAM and FLC, candidate dormancy master regulators expressed in dormant buds of Rosaceae fruit trees.
We found that DAM, a dormancy factor that is down-regulated upon low temperature encounter in rosaceous fruit trees, is involved in dormancy induction, dormancy maintenance, and dormancy awakening by altering abscisic acid and cytokinin accumulation. Furthermore, functional analysis showed that FLC, which is up-regulated in apple dormant buds upon encountering low temperature, may be involved in the inhibition of bud break under ecodormancy. These findings theoretically suggest that phytohormone treatments are effective in controlling dormancy.

Academic Significance and Societal Importance of the Research Achievements

低温要求性ならびに高温要求性制御に関与すると考えられるマスターレギュレーター候補因子を同定しそれらの機能の一端を解明した.特にDAM遺伝子が植物ホルモン代謝を制御することにより休眠をコントロールしている可能性を直接的に示したのは本研究がはじめてである.気候温暖化による休眠の攪乱は世界的に問題となっている.植物ホルモン代謝を制御することで休眠を人為制御できる可能性を示した点で,本研究成果は社会的意義がみとめられる.

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (29 results)

All 2021 2020 2019 2018

All Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 13 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 9 results,  Invited: 5 results) Book (2 results) Funded Workshop (1 results)

  • [Journal Article] Expression Analysis of Endodormancy and Flowering-related Genes in Greenhouse-cultivated Flowering Disorder Trees of Japanese pear (<i>Pyrus pyrifolia</i> Nakai) ‘Kosui’2021

    • Author(s)
      Tominaga, A. S. Kinose, M. Tai, M. Yahata and H. Yamane.
    • Journal Title

      The Horticulture Journal

      Volume: 90 Issue: 1 Pages: 38-47

    • DOI

      10.2503/hortj.UTD-233

    • NAID

      130007975571

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Allelic distribution at SNP loci within bud dormancy-related QTLs in Japanese apricot (Prunus mume) collections.2021

    • Author(s)
      Hsiang, T. W. Chen, H. Yamane, S. Nishiyama, R. Nakano and R. Tao.
    • Journal Title

      Acta Horticulturae

      Volume: 1312 Issue: 1312 Pages: 17-23

    • DOI

      10.17660/actahortic.2021.1312.3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] リンゴ花芽の休眠と花成進行に関連したDORMANCY-ASSOCIATED MADS-box, FLC-like遺伝子の発現挙動2020

    • Author(s)
      山根久代
    • Journal Title

      低温生物工学会誌

      Volume: 66 Pages: 17-25

    • NAID

      130007895015

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Identification of QTLs for chilling and heat requirements for bud dormancy release in Prunus mume and their co-localization with the DAM6 eQTL.2020

    • Author(s)
      Yamane, H., W. Chen, Y. Kitamura, T. Habu and R. Tao.
    • Journal Title

      Acta Horticulturae

      Volume: 1281 Issue: 1281 Pages: 585-591

    • DOI

      10.17660/actahortic.2020.1281.77

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of chilling accumulation on DORMANCY-ASSOCIATED MADS-box gene expressions in ‘Satonishiki’ sweet cherry.2019

    • Author(s)
      Masuda, K., H. Yamane, K. Ikeda, T. Testumura, M. Takai and R. Tao.
    • Journal Title

      Acta Horticulturae

      Volume: 1235 Issue: 1235 Pages: 421-426

    • DOI

      10.17660/actahortic.2019.1235.58

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional and expressional analyses of apple FLC-like in relation to dormancy progress and flower bud development2019

    • Author(s)
      Nishiyama S, Matsushita MC, Yamane H, Honda C, Okada K, Tamada Y, Moriya S, Tao R
    • Journal Title

      Tree Physiology

      Volume: tpz111 Issue: 4 Pages: 562-570

    • DOI

      10.1093/treephys/tpz111

    • NAID

      120007041694

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apple whole genome sequences: recent advances and new prospects2019

    • Author(s)
      Peace, C.P., L. Bianco, M. Troggio, E. van de Weg, N.P. Howard, A. Cornille, C-E. Durel, S. Myles, Z. Migicovsky, R.J. Schaffer, E. Costes, G. Fazio, H. Yamane, et al.,
    • Journal Title

      Hort. Res.

      Volume: 6 Issue: 1 Pages: 59-59

    • DOI

      10.1038/s41438-019-0141-7

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Overexpression of Prunus DAM6 inhibits growth, represses bud break competency of dormant buds and delays bud outgrowth in apple plants2019

    • Author(s)
      H. Yamane, M wada, C. Honda, T. Matsuura, Y Ikede, T, Hirayama, Y. Kitamura, M Gao-Takai and R. Tao
    • Journal Title

      PLoS ONE

      Volume: 14 Issue: 4 Pages: 1-24

    • DOI

      10.1371/journal.pone.0214788

    • NAID

      120006822496

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] xpression analysis of apple DAMs in 'Fuji' flower buds during flower bud development.2019

    • Author(s)
      Yamane, H., T. Takeuchi, M. Matsushita, K. Banno and R. Tao.
    • Journal Title

      Acta Horticulturae

      Volume: 1261 Issue: 1261 Pages: 143-148

    • DOI

      10.17660/actahortic.2019.1261.23

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of QTLs controlling chilling and heat requirements for dormancy release and bud break in Japanese apricot (Prunus mume).2018

    • Author(s)
      Kitamura, Y., T. Habu, H. Yamane*, S. Nishiyama, K. Kajita, T. Sobue, T. Kawai, K. Numaguchi, T. Nakazaki, A. Kitajima, and R. Tao
    • Journal Title

      Tree Genetics and Genomes

      Volume: 14 Issue: 2 Pages: 33-33

    • DOI

      10.1007/s11295-018-1243-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA-Sequencing analysis identifies genes associated with chilling-mediated endodormancy release in apple2018

    • Author(s)
      Takeuchi, T., M.C. Matsushita, S. Nishiyama, H. Yamane, K. Banno, and R. Tao
    • Journal Title

      J. Amer. Soc. Hort. Sci.

      Volume: 143 Issue: 3 Pages: 194-206

    • DOI

      10.21273/jashs04345-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of abscisic acid metabolism in endodormancy release in persimmon (Diospyros kaki)2018

    • Author(s)
      Iwasa, H., H. Yamane, M. Gao-Takai, A. Oikawa and R. Tao
    • Journal Title

      Acta Hort.

      Volume: 1208 Issue: 1208 Pages: 287-290

    • DOI

      10.17660/actahortic.2018.1208.39

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in plant hormone contents in Japanese apricot flower buds during prolonged chilling exposure2018

    • Author(s)
      Y. Kitamura, H. Yamane, M. Gao-Takai, R. Tao
    • Journal Title

      Acta Horticulture

      Volume: 1208 Issue: 1208 Pages: 251-256

    • DOI

      10.17660/actahortic.2018.1208.33

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of apple DORMANCY-ASSOCIATED MADS-box genes in buds during dormancy release2018

    • Author(s)
      Yamane, H. and R. Tao
    • Journal Title

      Acta Hort.

      Volume: 1208 Issue: 1208 Pages: 39-45

    • DOI

      10.17660/actahortic.2018.1208.6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characterization of the bud dormancy-related QTLs in Japanese apricot2020

    • Author(s)
      Hsiang, T.*., H. Yamane・W. Chen・S. Nishiyama・R. Nakano・R. Tao
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Diversity of flower developmental characteristics in low-chill cultivars in Rosaceae fruit trees grown in temperate climate2020

    • Author(s)
      Yamane*, H., T-F. Hsiang and R. Tao.
    • Organizer
      Annual meeting of American Society for Horticultural Science
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Allelic distribution at SNP loci within bud dormancy-related QTLs in Japanese apricot (Prunus mume) collections.2020

    • Author(s)
      Hsiang, T., W. Chen, H. Yamane, S. Nishiyama, R. Nakano and R. Tao.
    • Organizer
      3rd Asian Horticulture Congress
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 果樹の休眠の長短を決める遺伝的因子の探索2019

    • Author(s)
      山根久代
    • Organizer
      第64回低温生物工学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] バラ科果樹の低温要求性制御機構に関する研究2019

    • Author(s)
      山根久代・北村祐人
    • Organizer
      園芸学会平成31年度秋季大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Identification of QTLs for chilling and heat requirements for bud dormancy release in Prunus mume and their co-localization with the DAM6 eQTL2018

    • Author(s)
      Yamane, H., Y. Kitamura, T. Habu, Wenxing Chen and R. Tao
    • Organizer
      The 30th International Horticulture Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative observations of phenological characters of continuously flowering high-bush blueberry 'Blue Muffin' in temperate and subtropical climates2018

    • Author(s)
      Yamane*, H., K-T. Li, R. Matsuzaki, S. Ebihara, T-S. Li, M. Omori and R. Tao
    • Organizer
      The 30th International Horticulture Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic and molecular regulation of bud dormancy in Prunus mume2018

    • Author(s)
      Yamane, H
    • Organizer
      HZAU-NTU-KU Joint Horticulture Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenome analysis reveals the role of histone modifications in the chilling-regulated gene expression in apple dormant buds2018

    • Author(s)
      Chen, W., H. Yamane, Y. Tamada, M. Matsushita, Z. Luo and R. Tao
    • Organizer
      The 6th Plant Dormancy Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic and molecular regulation of bud dormancy in Prunus mume2018

    • Author(s)
      Yamane, H
    • Organizer
      The 6th Plant Dormancy Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of continuous flowering blueberry 'Blue Muffin' and the distinctive effect of hydrogen cyanamide on flowering2018

    • Author(s)
      Li, T., H. Yamane, M. Tomiyama and R. Tao
    • Organizer
      The 6th Plant Dormancy Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional characterization of apple FLC-like gene in winter dormant buds2018

    • Author(s)
      Matsushita, M., H. Yamane, S. Nishiyama, C. Honda, K. Okada, S. Moriya and R. Tao
    • Organizer
      The 6th Plant Dormancy Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Book] Functional genes in bud dormancy and impacts on plant breeding.. p. 101-117. In: Z. Gao (ed.). The Prunus mume Genome, Compendium of Plant Genomes.2019

    • Author(s)
      Kitamura, Y., W. Chen, H. Yamane and R. Tao.
    • Total Pages
      161
    • Publisher
      Springer
    • ISBN
      9783030107970
    • Related Report
      2019 Annual Research Report
  • [Book] Molecular and developmental biology: self-incompatibility. p. 119-135. In: Z. Gao (ed.). The Prunus mume Genome, Compendium of Plant Genomes.2019

    • Author(s)
      Yamane, H and R. Tao.
    • Total Pages
      161
    • Publisher
      Springer
    • ISBN
      9783030107970
    • Related Report
      2019 Annual Research Report
  • [Funded Workshop] The 6th Plant Dormancy Symposium2018

    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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