Developmental research of marine carotenoids on prevention of lifestyle-related diseases through their metabolite analysis and regulation of organ/cell interaction
| Project/Area Number |
18H02274
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 40040:Aquatic life science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岡松 優子 北海道大学, 獣医学研究院, 准教授 (90527178)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 海洋性カロテノイド / 生活習慣病予防 / アポカロテノイド / フコキサンチン / パラセントロン / 抗炎症作用 / アポー10’-フコキサンチナール / miRNA / β-カロテン開裂物 / アポアスタキサンチナール / 臓器・細胞間相互作用 / フコキサンチン開裂物 / 臓器間ネットワーク因子 |
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| Outline of Final Research Achievements |
A fucoxanthin-derived apocarotenoid, paracentrone was identified in liver and white adipose tissue of mice fed fucoxanthin. Further, dietary fucoxanthin attenuated hepatic fat accumulation and inflammation in the diet-induced nonalcoholic steatohepatitis model mice. Paracentrone suppressed the overexpression of inflammatory factors in activated RAW264.7 macrophages. Further, apo-10′-fucoxanthinal with an epoxide residue also suppressed pro-inflammatory cytokines through regulating IkB phosphorylation, while beta-apo-8′-carotenal did not suppress inflammation. In adipocytes and hepatocytes, paracentrone and apo-10′-fucoxanthinal downregulated the mRNA expression of proinflammatory mediators induced by the culture medium of activated RAW264.7 cells. These results suggest that apo-fucoxanthinoids derived from fucoxanthin regulate organ/cell interaction involved in chronic inflammation, which is basis of the development of lifestyle-related diseases.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、水産物に特徴的なカロテノイドの生活習慣病予防機構を明らかにするため、機能発現に関わる生体内代謝物の同定と抗炎症作用について検討した。ワカメなどの褐藻に含まれるフコキサンチンの新たな生体内代謝物としてパラセントロンを同定した。パラセントロンを含むフコキサンチン開裂物や代謝物は、活性化マクロファージや脂肪細胞、肝細胞に対して抗炎症作用を示し、生活習慣病の発症基盤となる慢性炎症に関わる細胞間相互作用を制御することを見出した。また、抗肥満作用や血糖値改善効果に加え、非アルコール性脂肪肝炎に対して予防効果が期待できることを示し、生活習慣病予防における水産物の有効性を示す成果を導いた。
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Report
(4 results)
Research Products
(29 results)
