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Life strategies of Candidate Phyla Radiation in deep subsurface

Research Project

Project/Area Number 18H02501
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionJapan Aerospace EXploration Agency (2019-2022)
Japan Agency for Marine-Earth Science and Technology (2018)

Principal Investigator

Suzuki Shino  国立研究開発法人宇宙航空研究開発機構, 宇宙科学研究所, 准教授 (10557002)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsメタゲノム / 蛇紋岩水系 / 極限環境微生物学 / アストロバイオロジー / 蛇紋岩化反応 / CPR / 極限環境微生物 / 始原生命 / 地下生命圏 / 極限微生物 / 比較メタゲノミクス / メタゲノミクス / OD1
Outline of Final Research Achievements

Candidate Phyla Radiation (CPR) bacteria are small in genome size, have introns in their rRNA genes, lack complete biosynthetic pathways for amino acids, nucleic acids, and lipids, and have no electron transport or respiratory metabolic systems, and are probably episymbiotic bacteria. In this study, we extracted high-quality genomic information on CPR from the database and performed evolutionary analysis to identify the characteristics on the translational and glycolytic systems. In addition, we also performed ecological and physiological studies on the microbial community of Hakuba Happo, where many CPR bacteria are present. The original plan was to conduct research focused on The Cedars in the U.S., but due to the impact of COVID19, the plan was changed.

Academic Significance and Societal Importance of the Research Achievements

CPR系統群は、バクテリアの多様性の15%以上を占めるにも関わらず、その生理・生態の理解があまり進んでいない。CPR細菌は、地下圏のみならず、口腔内や排水処理施設など、人間生活にも密接に関わる環境にも多く存在することが近年明らかにされており、これらの理解は、微生物ー人類の関わりにおける理解を深めることに貢献する。また、生命間でかなり保存性が高いと考えられた機能分子・機能システム(翻訳系や解糖系)においても、我々が想定していた以上にCPRは多様であることをが分かった。これらの多様な機能分子情報を用いて、その進化を捉えなおすことで、学術的意義のある成果を創出することが期待される。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (31 results)

All 2022 2021 2019 2018 Other

All Int'l Joint Research (7 results) Journal Article (9 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 15 results)

  • [Int'l Joint Research] University of Newfoundland(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] NASA Ames Research Center/University of Southern California/Naval Research Lab(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Delft University of Technology(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Southern California(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] J.Craig Venter Institute/University of Southern California(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Lisbon(ポルトガル)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Istituto Nazionale di Geofisica(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Unique H2-utilizing lithotrophy in serpentinite-hosted systems2022

    • Author(s)
      Nobu Masaru Konishi、Nakai Ryosuke、Tamazawa Satoshi、Mori Hiroshi、Toyoda Atsushi、Ijiri Akira、Suzuki Shino、Kurokawa Ken、Kamagata Yoichi、Tamaki Hideyuki
    • Journal Title

      The ISME Journal

      Volume: 17 Issue: 1 Pages: 95-104

    • DOI

      10.1038/s41396-022-01197-9

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] COSPAR Sample Safety Assessment Framework (SSAF)2022

    • Author(s)
      Kminek Gerhard、Shino Suzuki、他24名
    • Journal Title

      Astrobiology

      Volume: 22 Issue: S1 Pages: S-186-S-216

    • DOI

      10.1089/ast.2022.0017

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessing Geochemical Bioenergetics and Microbial Metabolisms at Three Terrestrial Sites of Serpentinization: The Tablelands (NL, CAN), The Cedars (CA, USA), and Aqua de Ney (CA, USA)2021

    • Author(s)
      Cook Melissa C.、Blank Jennifer G.、Suzuki Shino、Nealson Kenneth H.、Morrill Penny L.
    • Journal Title

      Journal of Geophysical Research: Biogeosciences

      Volume: 126 Issue: 6

    • DOI

      10.1029/2019jg005542

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs2021

    • Author(s)
      Bird Lina J.、Kuenen J. Gijs、Osburn Magdalena R.、Tomioka Naotaka、Ishii Shun’ichi、Barr Casey、Nealson Kenneth H.、Suzuki Shino
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 71 Issue: 8

    • DOI

      10.1099/ijsem.0.004945

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Geochemical Comparison of Three Terrestrial Sites of Serpentinization: The Tablelands, the Cedars, and Aqua de Ney2021

    • Author(s)
      Cook Melissa C.、Blank Jennifer G.、Rietze Amanda、Suzuki Shino、Nealson Kenneth H.、Morrill Penny L.
    • Journal Title

      Journal of Geophysical Research: Biogeosciences

      Volume: 126 Issue: 11

    • DOI

      10.1029/2021jg006316

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Origin of short-chain organic acids in serpentinite mud volcanoes of the Mariana convergent margin2019

    • Author(s)
      Philip Eickenbusch, Ken Takai, Olivier Sissman, Shino Suzuki, Catriona Menzies, Sanae Sakai, Pierre Sansjofre, Eiji Tasumi, Stefano M Bernasconi, Clemens Glombitza, Bo Barker Jorgensen, Yuki Morono, Mark Alexander Lever
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 1792-1792

    • DOI

      10.3389/fmicb.2019.01729

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mariana serpentinite mud volcanism exhumes subducted seamount materials: implications for the origin of life2019

    • Author(s)
      P. Fryer, C G. Wheat, T. Williams, S. Suzuki et al.
    • Journal Title

      Philosophical Transactions of the Royal Society A

      Volume: 378 Issue: 2165 Pages: 20180425-20180425

    • DOI

      10.1098/rsta.2018.0425

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genomic and in-situ Transcriptomic Characterization of the Candidate Phylum NPL-UPL2 From Highly Alkaline Highly Reducing Serpentinized Groundwater2018

    • Author(s)
      Suzuki Shino、Nealson Kenneth H.、Ishii Shun’ichi
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 646-649

    • DOI

      10.3389/fmicb.2018.03141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Linking serpentinization, hyperalkaline mineral waters and abiotic methane production in continental peridotites: an integrated hydrogeological-bio-geochemical model from the Cabe?o de Vide CH4-rich aquifer (Portugal)2018

    • Author(s)
      Marques J.M.、Etiope G.、Neves M.O.、Carreira P.M.、Rocha C.、Vance S.D.、Christensen L.、Miller A.Z.、Suzuki S.
    • Journal Title

      Applied Geochemistry

      Volume: 96 Pages: 287-301

    • DOI

      10.1016/j.apgeochem.2018.07.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 極限微生物の遺伝子機能を知る:初期生命進化の理解に向けて2022

    • Author(s)
      鈴木志野
    • Organizer
      日本分子生物学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Candidate Phyla Radiationの生理・生態・進化の理解がもたらすものとは?2022

    • Author(s)
      鈴木志野
    • Organizer
      微生物生態学会 自由集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ゲノム情報から紐解く蛇紋岩生命圏2022

    • Author(s)
      鈴木志野
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ゲノム情報から紐解く蛇紋岩生命圏2021

    • Author(s)
      鈴木志野
    • Organizer
      日本ゲノム微生物学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] How does an extreme environment drive microbial adaptive evolution? ; A case of integrative studies of deep subsurface setting2021

    • Author(s)
      Shino Suzuki
    • Organizer
      10th ELSI Symposium
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Life in serpentinized setting; Implications for metabolic strategies of early life2021

    • Author(s)
      Shino Suzuki
    • Organizer
      Life in the Universe 2021
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 極限環境に生きる生命から考える地球外生命の可能性2021

    • Author(s)
      鈴木志野
    • Organizer
      大学共同利用機関シンポジウム2021
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Carbon fixation in highly alkaline and highly reducing serpentinized setting2021

    • Author(s)
      Shino Suzuki
    • Organizer
      日本進化学会第23回東京大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 化学特性の異なる蛇紋岩化反応サイトにおける 比較ゲノム微生物学的解析2021

    • Author(s)
      鈴木志野, 石井俊一, ケンニールソン
    • Organizer
      JpGU
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 強アルカリ・超還元的な蛇紋岩水系に生きる微生物の世界2019

    • Author(s)
      鈴木志野
    • Organizer
      第二回ExCELLSシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 蛇紋岩生命圏の世界2019

    • Author(s)
      鈴木志野
    • Organizer
      日本微生物生態学会 奨励賞受賞講演
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 岩に生かされる生命たち:地球深部強アルカリ環境をすみかとする微生物の生き様に迫る2018

    • Author(s)
      鈴木志野
    • Organizer
      第61回海中海底工学フォーラム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Unusual metabolic strategies identified in a hyperalkaliphilic microbial community associated with the serpentinization2018

    • Author(s)
      Shino Suzuki
    • Organizer
      日本地球惑星科学連合2018年大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Extensive Loss of Methanogenic Pathway: Adaptive Strategy of Methanosarcinales to the Hyperalkaline Setting Associated with Serpentinization2018

    • Author(s)
      Shino Suzuki
    • Organizer
      Gordon Research Conference
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 初期地球類似環境に見られる特異な微生物代謝システム2018

    • Author(s)
      鈴木志野
    • Organizer
      細胞を創る研究会11.0
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2024-01-30  

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