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Next generation therapy of podocytopathy developed by renal organoid

Research Project

Project/Area Number 18H02827
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 53040:Nephrology-related
Research InstitutionTokai University

Principal Investigator

MATSUSAKA Taiji  東海大学, 医学部, 教授 (50317749)

Co-Investigator(Kenkyū-buntansha) 荒岡 利和  京都大学, iPS細胞研究所, 特定研究員 (40437661)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords腎臓病 / オルガノイド / ポドサイト / ネフローゼ症候群 / ネフロン前駆細胞
Outline of Final Research Achievements

Kidney cells change dramatically their character when they are cultured in dishes. Utilizing recently developed techniques, we generated kidney organoids from mouse kidney progenitor cells and established a method to induce injury and analyze the responses. We previously found that when a portion of kidney cells are injured, other cells are secondarily damaged in a mouse model. In the present study, we found this damage spreading is reproduced in kidney organoids.

Academic Significance and Societal Importance of the Research Achievements

試験管内で腎臓病を再現可能になったので、薬剤の効果検証やスクリーニングが容易になり、腎臓病の治療法の開発に役立つと期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (28 results)

All 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (19 results) (of which Int'l Joint Research: 11 results,  Invited: 1 results)

  • [Journal Article] Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells2020

    • Author(s)
      Matsuda J, Takahashi A, Takabatake Y, Sakai S, Minami S, Yamamoto T, Fujimura R, Namba-Hamano T, Yonishi H, Nakamura J, Kimura T, Kaimori JY, Matsui I, Takahashi M, Nakao M, Izumi Y, Bamba T, Matsusaka T, Niimura F, Yanagita M, Yoshimori T, Isaka Y.
    • Journal Title

      Autophagy

      Volume: - Pages: 1-16

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biphasic MIF and SDF1 expression during podocyte injury promote CD44-mediated glomerular parietal cell migration in focal segmental glomerulosclerosis2020

    • Author(s)
      Ito N, Sakamoto K, Hikichi C, Matsusaka T, Nagata M.
    • Journal Title

      Am J Physiol Renal Physiol

      Volume: 318(3)

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Global polysome analysis of normal and injured podocytes2019

    • Author(s)
      Okabe Masahiro, Motojima Masaru, Miyazaki Yoich, Pastan Ira, Yokoo Takashi, Matsusaka Taiji
    • Journal Title

      American Journal of Physiology-Renal Physiology

      Volume: 316 Issue: 2 Pages: F241-F252

    • DOI

      10.1152/ajprenal.00115.2018

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proximal Tubule Autophagy Differs in Type 1 and 2 Diabetes2019

    • Author(s)
      Sakai S, Yamamoto T, Takabatake Y, Takahashi A, Namba-Hamano T, Minami S, Fujimura R, Yonishi H, Matsuda J, Hesaka A, Matsui I, Matsusaka T, Niimura F, Yanagita M, Isaka Y.
    • Journal Title

      J Am Soc Nephrol

      Volume: 30(6) Issue: 6 Pages: 929-945

    • DOI

      10.1681/asn.2018100983

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent Manner2019

    • Author(s)
      Koizumi Masahiro、Ueda Kohei、Niimura Fumio、Nishiyama Akira、Yanagita Motoko、Saito Akihiko、Pastan Ira、Fujita Toshiro、Fukagawa Masafumi、Matsusaka Taiji
    • Journal Title

      Hypertension

      Volume: 74 Issue: 3 Pages: 509-517

    • DOI

      10.1161/hypertensionaha.118.12352

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Possible role of complement factor H in podocytes in clearing glomerular subendothelial immune complex deposits2019

    • Author(s)
      Zoshima T, Hara S, Yamagishi M, Pastan I, Matsusaka T, Kawano M, Nagata M.
    • Journal Title

      Sci Rep

      Volume: 9(1) Issue: 1 Pages: 7857-7857

    • DOI

      10.1038/s41598-019-44380-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autophagy protects kidney from phosphate-induced mitochondrial injury2019

    • Author(s)
      Fujimura R, Yamamoto T, Takabatake Y, Takahashi A, Namba-Hamano T, Minami S, Sakai S, Matsuda J, Hesaka A, Yonishi H, Nakamura J, Matsui I, Matsusaka T, Niimura F, Yanagita M, Isaka Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: S0006-291X(20) Issue: 3 Pages: 30220-5

    • DOI

      10.1016/j.bbrc.2020.01.137

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ポドサイトパチー2019

    • Author(s)
      岡部匡裕 松阪泰二
    • Journal Title

      日本内科学会雑誌

      Volume: 108 Pages: 116-125

    • NAID

      130007783828

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of the differential expression of ZO-1 and ZO-2 on podocyte structure and function.2018

    • Author(s)
      Itoh M, Nakadate K, Matsusaka T, Hunziker W, Sugimoto H
    • Journal Title

      Genes Cells

      Volume: 23 Issue: 7 Pages: 546-556

    • DOI

      10.1111/gtc.12598

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マウス腎オルガノイドを用いた選択的ポドサイト障害モデル2020

    • Author(s)
      宇田川智宏、田中景子、荒岡利和、長船健二、松阪泰二
    • Organizer
      日本腎臓学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] P2X7 Expressed in Injured Podocytes May Spread the Kidney Injury Through Caspase 32019

    • Author(s)
      Yamamoto kazuyoshi,MatsusakaTaiji
    • Organizer
      American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] C-Type Lectin-Like Receptor (CLEC)-2, the Ligand of Podoplanin, Facilitates Motility of Podocytes2019

    • Author(s)
      Tanaka Keiko,MatsusakaTaiji
    • Organizer
      American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Kidney Organoid Model of Selective Podocyte Injury2019

    • Author(s)
      UdagawaTomohiro,MatsusakaTaiji
    • Organizer
      American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Angiotensin II Receptor Blocker Blocks Spreading Podocyte Damage in a Partial Podocytectomy Model2019

    • Author(s)
      Okabe Masahiro,MatsusakaTaiji
    • Organizer
      American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Glomerular Filtrate Promotes Cell Detachment in Podocyte Injury2018

    • Author(s)
      Nobuyuki Saga, Naoko Ito, Kazuo Sakamoto, Taiji Matsusaka, Michio Nagata
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Scavenging Reactive Dicarbonyls Improves Renal Injury: Role of Urinary Isolevuglandin-Modied Lipoproteins and Renal Lymphangiogenesis.2018

    • Author(s)
      Jianyong Zhong, Haichun Yang, Taiji Matsusaka, Agnes B. Fogo, Macrae F. Linton, Valentina Kon, Sean S. Davies
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Varying AKI Types Affect Parietal Epithelial Cell Function with Different Impact on Tubuloglomerular Cross-Talk2018

    • Author(s)
      Jiayi Wang, Xin Li, Ming-Zhi Zhang, Raymond C. Harris, Taiji Matsusaka, Haichun Yang, Agnes B. Fogo
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Autophagy Protects Kidney Proximal Tubules from Phosphate-Mediated Mitochondrial Dysfunction2018

    • Author(s)
      Ryuta Fujimura, Takeshi Yamamoto, Yoshitsugu Takabatake, Satoshi Minami, Atsushi Takahashi, Tomoko Namba, Jun Matsuda, Shinsuke Sakai, Isao Matsui, Fumio Niimura, Taiji Matsusaka, Yoshitaka Isaka
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcription Factor Dach1 in Podocytes2018

    • Author(s)
      Masahiro Okabe,Yoichi Miyazaki, Takashi Yokoo, Taiji Matsusaka.
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Podocyte-Specific Knockdown of PAI-1 Protects Against Podocyte Injury2018

    • Author(s)
      Jianyong Zhong, Taiji Matsusaka, Haichun Yang, Agnes B. Fogo
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] P2X7-ATP May Mediate Propagation of Podocyte Damage2018

    • Author(s)
      Kazuyoshi Yamamoto, Masahiro Okabe, Taiji Matsusaka, Takashi Yokoo
    • Organizer
      ASN Kidney Week
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウスを用いた腎臓病の研究2018

    • Author(s)
      松阪泰二
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ポドサイト障害におけるubiquitin specific protease 40の役割2018

    • Author(s)
      高橋昌兵、羽田伊知郎、三上直明、楊國昌、松阪泰二
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 正常ポドサイトに強発現する転写因子Dach12018

    • Author(s)
      岡部匡裕、宮崎陽一、横尾隆、松阪泰二
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] オートファジーは高リン負荷による腎尿細管ミトコンドリア傷害に対抗する2018

    • Author(s)
      藤村龍太、山本毅士、高畠義嗣、南聡、酒井晋介、高橋篤史、難波倫子、松田潤、松井功、坂口悠介、濱野高行、新村文男、松阪泰二、猪阪善隆
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MMP-10欠損によるアルドステロン投与GC-A ノックアウトマウスの腎病変の改善2018

    • Author(s)
      大崎啓介、加藤有希子、戸田尚宏、石井輝、森潔、松阪泰二、向山政志、柳田素子、横井秀基
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Foxc1/2が欠損するとS-shaped bodyの形成が阻害される2018

    • Author(s)
      小泉賢洋、深川雅史、松阪泰二、本島英
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] オートファジー抑制因子Rubiconの臓器特異性:オートファジー亢進が臓器障害に与えうる保護効果と副作用2018

    • Author(s)
      藤村龍太、山本毅士、高畠義嗣、松田潤、南聡、酒井晋介、高橋篤史、難波倫子、新村文男、松阪泰二、吉森保、猪阪善隆
    • Organizer
      日本腎臓学会総会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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