Development of gene-modified culture cells that reflect the specific infection events of arthropod-borne viruses
Project/Area Number |
18H02856
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 54030:Infectious disease medicine-related
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Isawa Haruhiko 国立感染症研究所, 昆虫医科学部, 室長 (90370965)
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Co-Investigator(Kenkyū-buntansha) |
深澤 征義 国立感染症研究所, 細胞化学部, 部長 (20291130)
駒形 修 国立感染症研究所, 昆虫医科学部, 主任研究官 (20435712)
沢辺 京子 国立感染症研究所, 昆虫医科学部, 主任研究官 (10215923)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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Keywords | 節足動物媒介性ウイルス / 宿主細胞 / ゲノム科学 / ゲノム編集 / バイオインフォマティクス |
Outline of Final Research Achievements |
We attempted to develop mosquito-derived cultured cell lines useful for mosquito-borne virus research, and succeeded in establishing new cell lines from Aedes aegypti and Aedes albopictus. These cell lines were found to be differentially susceptible to various mosquito-borne viruses. De novo genome assembly was conducted for the NIID-CTR cell culture, which was derived from Culex tritaeniorhynchus. Obtained biological resources will benefit studies of population genetics and molecular mechanisms for virus transmission of this important vector mosquito. We found that Huh7.5.1-8 cells, isolated from human hepatoma Huh 7 cells by us, were highly permissive to arthropod-borne viruses such as Japanese encephalitis virus, Sindbis virus, and severe fever with thrombocytopenia syndrome virus.
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Academic Significance and Societal Importance of the Research Achievements |
本研究により樹立に成功した蚊由来培養細胞系は、デングウイルス・ジカウイルス・黄熱ウイルスなど、蚊媒介性ヒト病原ウイルスに対する感受性や宿主範囲ならびに媒介能を規定する分子基盤解明に役立つと期待される。本研究で得られた結果から、NIID-CTR細胞のモデル細胞としての妥当性が示され、コガタアカイエカの生態やウイルスの媒介能に関する分子基盤を明らかにするための重要な情報基盤となると期待される。 我々が分離したHuh7.5.1-8細胞は、既存の昆虫及びダニ媒介性ウイルスの簡便な分離・検出に利用できることが期待され、また、未知の昆虫及びダニ媒介性ウイルスの分離・検出にも有用である可能性がある。
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Detection of Jingmenviruses in Japan with Evidence of Vertical Transmission in Ticks2021
Author(s)
Kobayashi Daisuke、Kuwata Ryusei、Kimura Toshiya、Shimoda Hiroshi、Fujita Ryosuke、Faizah Astri Nur、Kai Izumi、Matsumura Ryo、Kuroda Yudai、Watanabe Shumpei、Kuniyoshi Sawako、Yamauchi Takeo、Watanabe Mamoru、Higa Yukiko、Hayashi Toshihiko、Shinomiya Hiroto、Maeda Ken、Kasai Shinji、Sawabe Kyoko、Isawa Haruhiko
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Journal Title
Viruses
Volume: 13
Issue: 12
Pages: 2547-2547
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] アオダイショウ由来培養細胞の性状解析2021
Author(s)
鍬田龍星, 黒木俊郎, 松井利康, 石嶋慧多, 立本完吾, 小林大介, 伊澤晴彦, 高野愛, 下田宙, 藤井ひかる, 畑明寿, 宇根有美, 森川茂, 前田健, 吉川泰弘
Organizer
第73回日本衛生動物学会大会
Related Report
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[Presentation] Arbovirus surveillance and Japanese encephalitis virus genotype IV isolation from Culex vishnui mosquitoes collected on Bali Island, Indonesia2021
Author(s)
Faizah AN, Kobayashi D, Maekawa Y, Amoa-Bosompem M, Fauziyah S, Mulyatno KC, Subekti S, Rohmah EA, Lusida MI, Mori Y, Miura K, Hirayama K, Isawa H, Sawabe K
Organizer
American Society of Tropical Medicine & Hygiene 70th Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] Virome analysis of Japanese encephalitis vector, Culex tritaeniorhynchus, collected from three prefectures in Japan2019
Author(s)
Astri Nur Faizah, Daisuke Kobayashi, Michael Amoa-Bosompem, Haruhiko Isawa, Kyeong Soon Kim, Mamoru Watanabe, Kozue Miura, Kazuhiro Hirayama, Kyoko Sawabe
Organizer
第71回日本衛生動物学会大会
Related Report
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[Presentation] 蚊ゲノムに内在化したウイルス様配列の遺伝子構造解析2019
Author(s)
鳥井駿, 鍬田龍星, Spriyono, 下田宙,Thanmaporn Phichitraslip,Sathaporn Jittapalapong, Worawut Rerkamnuaychoke,Upik Kesumawati Hadi,Agus Setiyono,Srihadi Agungpriyono,糸川健太郎,伊澤晴彦,沢辺京子,前田健
Organizer
第71回日本衛生動物学会大会
Related Report
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[Presentation] COMPARING THE COMPETENCE OF GHANAIAN AND VIETNAMESE AEDES MOSQUITOES AS VECTORS OF THE DENGUE VIRUS2019
Author(s)
Michael Amoa-Bosompem, Daisuke Kobayashi, Katsunori Murota, Astri Nur Faizah, Kentaro Itokawa, Mitsuko Ohashi, Samuel Dadzie, Kofi J. Bonney, Toshinori Sasaki, Haruhiko Isawa, Kyoko Sawabe, Shiroh Iwanaga
Organizer
ASTMH 68th Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] DISEASE SURVEILLANCE AND VIROME ANALYSIS STUDY OF JAPANESE ENCEPHALITIS VECTOR, CULEX TRITAENIORHYNCHUS, COLLECTED FROM THREE PREFECTURES IN JAPAN2019
Author(s)
Astri Nur Faizah, Daisuke Kobayashi, Michael Amoa-Bosompem, Haruhiko Isawa, Kyeong Soon Kim, Mamoru Watanabe, Kozue Miura, Kazuhiro Hirayama, Kyoko Sawabe
Organizer
ASTMH 68th Annual Meeting
Related Report
Int'l Joint Research