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Biological effect and preventive method for human serum albumin binding to transboundary air borne PM2.5.

Research Project

Project/Area Number 18H03039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 58020:Hygiene and public health-related: including laboratory approach
Research InstitutionKochi University (2019-2020)
Okayama University (2018)

Principal Investigator

Ogino Keiki  高知大学, 医学部, 特任教授 (70204104)

Co-Investigator(Kenkyū-buntansha) 尾長谷 靖  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (40399762)
菅沼 成文  高知大学, 教育研究部医療学系連携医学部門, 教授 (50313747)
栄徳 勝光  高知大学, 教育研究部医療学系連携医学部門, 講師 (50552733)
荻野 学芳  高知大学, 医学部, 客員助教 (70614204)
荻野 志穂奈  高知大学, 医学部, 客員助教 (70746685)
市村 宏  金沢大学, 医学系, 教授 (10264756)
長岡 憲次郎  岡山大学, 医歯薬学総合研究科, 助教 (40752374)
高柴 正悟  岡山大学, 医歯薬学総合研究科, 教授 (50226768)
伊藤 達男  岡山大学, 医歯薬学総合研究科, 助教 (80789123)
早川 和一  金沢大学, その他部局等, 名誉教授 (40115267)
中村 裕之  金沢大学, 医学系, 教授 (30231476)
上原 孝  岡山大学, 医歯薬学総合研究科, 教授 (00261321)
浜田 博喜  岡山理科大学, 理学部, 教授 (10164914)
岡野 光博  国際医療福祉大学, 医学部, 教授 (60304359)
江口 依里  岡山大学, 医歯薬学総合研究科, 助教 (60635118)
竹本 圭  岡山大学, 医歯薬学総合研究科, 助教 (60723252)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
KeywordsPM2.5 / ヒトアルブミン / チロシンニトロ化 / 好酸球炎症 / 大気環境 / SOD2 / clathrin / 新型コロナウイルス / ナノPM / ニトロチロシン / エンドサイトーシス / 自己抗体 / ナノPM / 磁性
Outline of Final Research Achievements

We discovered for the first time in the world that human albumin (hAlb) is bound to atmospheric fine particulate matter (PM2.5), and investigated its origin and biological effects. We found that hAlb, which is excreted from humans, binds to PM2.5, remains on the surface of the earth without being degraded, and may be dispersed into the atmosphere during winter when humidity is low. hAlb binds to PM2.5, enters cells through clathrin-dependent endocytosis, and induces oxidative stress. Furthermore, hAlb bound to PM2.5 reacted with O3 and NO2 in the air to form nitrated hAlb, which caused eosinophilia in bronchi via IL-5 and eotaxin-1.

Academic Significance and Societal Importance of the Research Achievements

大気中のPM2.5からヒトアルブミンを世界で最初に同定し、その一部が修飾されアレルゲンになる可能性を見出したことは、大気環境の新しいマーカーを見つけたことになる可能性が有り、学術的価値は大きい。このことは、PM2.5の生体影響の研究に重要な情報となるばかりか、大気環境の指標としてさらなる研究の発展に貢献する可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (13 results)

All 2021 2019 2018

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (7 results)

  • [Journal Article] The identification of tyrosine-nitrated human serum albumin in airborne particulate matter from Japan2021

    • Author(s)
      Ogino Noriyoshi、Ogino Keiki、Eitoku Masamitsu、Suganuma Narufumi、Nagaoka Kenjiro、Kuramitsu Yasuhiro
    • Journal Title

      Atmospheric Pollution Research

      Volume: 12 Issue: 5 Pages: 101050-101050

    • DOI

      10.1016/j.apr.2021.03.013

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice2019

    • Author(s)
      Nagaoka Kenjiro、Ogino Keiki、Ogino Noriyoshi、Ito Tatsuo、Takemoto Kei、Ogino Shihona、Seki Yuka、Hamada Hiroki、Fujikura Yoshihisa
    • Journal Title

      Environmental Toxicology

      Volume: 34 Issue: 7 Pages: 836-843

    • DOI

      10.1002/tox.22751

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of 3-Nitrotyrosine in Atmospheric Environments via a Highperformance Liquid Chromatography-electrochemical Detector System2019

    • Author(s)
      Ito T, Ogino K, Nagaoka K, Takemoto K, Nishiyama R, Shimizu Y
    • Journal Title

      JoVE

      Volume: 143 Issue: 143

    • DOI

      10.3791/58371

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice.2019

    • Author(s)
      Nagaoka K, Ogino K, Ogino N, Ito T, Takemoto K, Ogino S, Seki Y, Hamada H, Fujikura Y.
    • Journal Title

      Environ Toxicol

      Volume: accepted.

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship of particulate matter and ozone with 3-nitrotyrosine in the atmosphere.2018

    • Author(s)
      Ito T, Ogino K, Nagaoka K, Takemoto K
    • Journal Title

      Environ Pollut.

      Volume: 236C Pages: 948-952

    • DOI

      10.1016/j.envpol.2017.10.069

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of PM2.5-bound protein and metals in PM2.5-induced allergic airway inflammation in mice.2018

    • Author(s)
      Ogino K, Nagaoka K, Ito T, Takemoto K, Okuda T, Nakayama SF, Ogino N, Seki Y, Hamada H, Takashiba S, Fujikura Y.
    • Journal Title

      Inhal Toxicol.

      Volume: 30 Issue: 13-14 Pages: 498-508

    • DOI

      10.1080/08958378.2018.1561769

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大気中のparticulate matter(PM)に含有されるタンパク質の同定2021

    • Author(s)
      荻野景規、荻野学芳、長岡憲次郎、栄徳勝光、菅沼成文
    • Organizer
      第91回日本衛生学会学術総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] PM2.5 曝露による気道上皮細胞の粘液発現.2019

    • Author(s)
      長岡憲次郎、荻野学芳、竹本圭、伊藤達男、笹岡香織、荻野景規
    • Organizer
      第89回日本衛生学会学術総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PM2.5のタンパク質を介した生体影響2019

    • Author(s)
      荻野 景規、長岡 憲次郎、伊藤 達男、松浦 有希
    • Organizer
      第26回クロマトグラフィーシンボジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大気中PM タンパク質の3- ニトロチロシン生成と大気汚染物質の関連性.2018

    • Author(s)
      伊藤達男、長岡憲次郎、竹本圭、荻野景規
    • Organizer
      第88回日本衛生学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気道上皮細胞におけるPM2.5 曝露に対する粘液産生の影響2018

    • Author(s)
      長岡憲次郎、伊藤達男、竹本圭、江口依里、荻野景規
    • Organizer
      第88回日本衛生学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 浮遊粒子状物質とニトロ化タンパクの共曝露によるマウス気道炎症の発症.2018

    • Author(s)
      竹本圭、長岡憲次郎、伊藤達男、荻野景規
    • Organizer
      第88回日本衛生学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] PM2.5の培養細胞における毒性学的評価2018

    • Author(s)
      長岡憲次郎、伊藤達男、竹本圭、江口依里、荻野景規
    • Organizer
      第16回日本予防医学会学術総会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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