Development of spatial and time specific physio therapy using broad dielectric spectroscopy

• Project/Area Number: 18H03128
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Kyoto University
• Principal Investigator: Aoyama Tomoki 京都大学, 医学研究科, 教授 (90378886)
• Co-Investigator(Kenkyū-buntansha): 新屋敷 直木 東海大学, 理学部, 教授 (00266363) ; 池口 良輔 京都大学, 医学研究科, 准教授 (80437201)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 物理療法 / 電気刺激 / 神経再生 / aaaa / 物理治療 / 定位的 / 広帯域誘電法

Outline of Final Research Achievements

On peripheral injury, the protein and water contents changes in each regeneration steps. It means that physical phenomenon in each steps are differed when electrically stimulated. But current electrical stimulation condition is provided in same condition. The broad dielectric spectroscopy is a high through put screening device using impedance method for measuring the electrical response when electric stimulation broadly. The purpose of the study is to find a suitable electrical stimulation condition in each steps of nerve regeneration using the broad dielectric spectroscopy.

Academic Significance and Societal Importance of the Research Achievements

これまで電気治療をはじめとする物理療法は、教科書に記載されているような組織深度を基準に、経験的にその刺激条件の検証が行われてきた。治癒を促す組織の再生の程度や時期によって、その条件は変化するものであるが、そのような視点ではこれまで検証されてこなかった。すなわち今回のテーマである末梢神経障害の病態に沿って検証された結果ではないことから良好とは言えない臨床結果を示している。本申請においては細胞や組織を、水分子含んだタンパク集合体と捉え、そのタンパク集合体の時期、組成に応じた刺激条件を検証するといった手法であり、病理的なロジックモデルに即した再生促進条件を検証するものである。

Research Products

• [Journal Article] Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy. (2021)
  • Author(s): Otagiri R, Kawai H, Takatsuka M, Shinyashiki N, Ito A, Ikeguchi R, Aoyama T.
  • Journal Title: Plos One Volume: 16
  • DOI: 10.1371/journal.pone.0252589
  • Peer Reviewed / Open Access
• [Journal Article] Relative antigenicity of components in vascularized composite allotransplants: An experimental study of microRNAs expression in rat hind limb transplantation model. (2019)
  • Author(s): Oda H, Ikeguchi R, Aoyama T, Ohta S, Noguchi T, Kaizawa Y, Yurie H, Takeuchi H, Mitsuzawa S, Yamamoto K, Matsuda S.
  • Journal Title: Microsurgery Volume: 39 Pages: 340-348
  • DOI: 10.1002/micr.30408
  • Peer Reviewed / Open Access
• [Journal Article] The efficacy of a scaffold-free bio 3D conduit developed from autologous dermal fibroblasts on peripheral nerve regeneration in a canine ulnar nerve injury model: A preclinical proof-of-concept study (2019)
  • Author(s): Mitsuzawa S, Ikeguchi R, Aoyama T, Takeuchi H, Yurie H, Oda H, Ohta S, Ushimaru M, Ito T, Tanaka M, Kunitomi Y, Tsuji M, Akieda S, Nakayama K, Matsuda S.
  • Journal Title: Cell Transplant Volume: 28 Pages: 1231-1241
  • DOI: 10.1177/0963689719855346
  • NAID: 120006951683
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cell-derived mesenchymal stem cells prolong hind limb survival in a rat vascularized composite allotransplantation model. (2019)
  • Author(s): Mitsuzawa S, Ikeguchi R, Aoyama T, Ando M, Takeuchi H, Yurie H, Oda H, Noguchi T, Ohta S, Zhao C, Ikeya M, Matsuda S
  • Journal Title: Microsurgery Volume: 20 Pages: 737-747
  • DOI: 10.1002/micr.30507
  • Peer Reviewed / Open Access
• [Journal Article] Acute Effects of Capacitive and Resistive Electric Transfer (CRet) on the Achilles Tendon (2019)
  • Author(s): Bito T, Tashiro Y, Suzuki Y, Kajiwara Y, Zeidan H, Kawagoe M, Sonoda T, Nakayama Y, Yokota Y, Shimoura K, Tatsumi M, Nakai K, Nishida Y, Yoshimi S, Tsuboyama T, Aoyama T.
  • Journal Title: Electromagn Biol Med Volume: 38 Pages: 48-54
  • DOI: 10.1080/15368378.2020.1737803
  • Peer Reviewed
• [Journal Article] Three-dimensional motion analysis for comprehensive understanding of gait characteristics after sciatic nerve lesion in rodents (2019)
  • Author(s): Tajino Junichi、Ito Akira、Tanima Momoko、Yamaguchi Shoki、Iijima Hirotaka、Nakahata Akihiro、Kiyan Wataru、Aoyama Tomoki、Kuroki Hiroshi
  • Journal Title: Sci Rep Volume: 8 Pages: 13585-13585
  • DOI: 10.1038/s41598-018-31579-z
  • NAID: 120006529728
  • Peer Reviewed / Open Access
• [Journal Article] Acute effects of capacitive and resistive electric transfer (CRet) on the achilles tendon. (2019)
  • Author(s): Bito T, Tashiro Y, Suzuki Y, Kajiwara Y, Zeidan H, Kawagoe M, Sonoda T, Nakayama Y, Yokota Y, Shimoura K, Tatsumi M, Nakai K, Nishida Y, Yoshimi S, Tsuboyama T, Aoyama T.
  • Journal Title: Electromagn Biol Med Volume: 38(1) Pages: 48-54
  • DOI: 10.1080/15368378.2019.1567525
  • Peer Reviewed / Open Access
• [Journal Article] Functional evaluation outcomes correlate with histomorphometric changes in the rat sciatic nerve crush injury model: A comparison between sciatic functional index and kinematic analysis (2018)
  • Author(s): Wang Tianshu、Ito Akira、Aoyama Tomoki、Nakahara Ryo、Nakahata Akihiro、Ji Xiang、Zhang Jue、Kawai Hideki、Kuroki Hiroshi
  • Journal Title: PLoS One Volume: 13 Pages: 1-9
  • DOI: 10.1371/journal.pone.0208985
  • NAID: 120006546016
  • Peer Reviewed / Open Access
• [Presentation] Kinematic analysis for understanding functional changes over time and correlations between functional and histomorphometric data following sciatic nerve crush injury in rats (2019)
  • Author(s): Tianshu Wang, Akira Ito, Ryo Nakahara, Akihiro Nakahata, Xiang Ji, Jue Zhang, Hideki Kawai, Naoko Kubo, Hiroshi Kuroki, Tomoki Aoyama
  • Organizer: 13th International Society of Physical and Rehabilitation Medicine World Congress
  • Int'l Joint Research
• [Presentation] Immediate Effects of Transcutaneous Electrical Nerve Stimulation on Pain and Physical Performance during Physical Functional Tests in Individuals with Pre-Radiographic Knee Osteoarthritis. (2019)
  • Author(s): Kanako Shimoura, Hirotaka Iijima, Yusuke Suzuki, Tomoki Aoyama
  • Organizer: 13th International Society of Physical and Rehabilitation Medicine World Congress.
  • Int'l Joint Research
• [Presentation] The Efficacy of a scaffold-free bio 3D conduit on peripheral nerve regeneration. (2018)
  • Author(s): Aoyama T
  • Organizer: 5th TERMIS World Cogress 2018
  • Int'l Joint Research / Invited
• [Presentation] The efficacy of a scaffold free Bio 3D conduit developed from dermal fibroblasts on peripheral nerve injury in a canine ulnar nerve injury model. (2018)
  • Author(s): Mitsuzawa S,Ikeguchi R, Aoyama T, Takeuchi H, Yurie H, Oda H, Ohta S, Tsuji M, Akieda S, Nakayama K, Matsuda S.
  • Organizer: 5th TERMIS World Cogress 2018
  • Int'l Joint Research
• [Presentation] Peripheral nerve regeneration using a bio 3D conduit from undifferentiated bone marrow stromal cells (2018)
  • Author(s): Hirofumi Yurie, Ryosuke Ikeguchi, Tomoki Aoyama, Souichi Ohta, Koichi Nakayama, Shuichi Matsuda.
  • Organizer: ASSH 2018
  • Int'l Joint Research
• [Presentation] Ultrasound therapy suppresses inflammatory gene expression in an injured peripheral nerve after sciatic nerve crush injury in rats. (2018)
  • Author(s): Akira Ito, Tianshu Wang, Ryo Nakahara, Hideki Kawai, Akihiro Nakahata, Jue Zhang, Naoko Kubo, Junichi Tajino, Tomoki Aoyama, Hiroshi Kuroki. Ultrasound therapy suppresses inflammatory gene expression in an injured peripheral nerve after sciatic nerve crush injury in rats.
  • Organizer: 7th International Symposium on Regenerative Rehabilitation
  • Int'l Joint Research
• [Presentation] Effectiveness of rehabilitation after cell transplantation for peripheral nerve regeneration: protocol for a systematic review. (2018)
  • Author(s): Hideki Kawai, Akira Ito, Tomoki Aoyama, Hiroshi Kuroki
  • Organizer: 7th International Symposium on Regenerative Rehabilitation
  • Int'l Joint Research
• [Presentation] The correlation of kinematic changes with histomorphometric data in the rat sciatic nerve crush injury model. (2018)
  • Author(s): Tianshu Wang, Akira Ito, Ryo Nakahara, Akihiro Nakahata, Xiang Ji, Jue Zhang, Hideki Kawai, Naoko Kubo, Hiroshi Kuroki, Tomoki Aoyama.
  • Organizer: 7th International Symposium on Regenerative Rehabilitation
  • Int'l Joint Research