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Consolidated bioremediation of heavy metal contamination using biocementation and biomass

Research Project

Project/Area Number 18H03395
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionHokkaido University

Principal Investigator

Kazunori Nakashima  北海道大学, 工学研究院, 准教授 (50540358)

Co-Investigator(Kenkyū-buntansha) 川崎 了  北海道大学, 工学研究院, 教授 (00304022)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywordsバイオセメント / 重金属汚染 / バイオレメディエーション / 金属イオン吸着 / バイオミネラル / 環境修復 / 微生物
Outline of Final Research Achievements

The objective of this study is to develop a system that can immobilize soil contaminated with high concentration of heavy metals using bio-cement technique, and completely remove a trace amount of heavy-metal ions in water using heavy-metal-binding biomass. Precipitation of CaCO3 using urease-producing bacteria was successfully applied for solidification and immobilization of heavy metals in a model and actual contaminated soil samples. Furthermore, bio-cement combined with organic polymers was developed to increase the strength of biocemented sample. Novel heavy metal adsorbents based on protein and natural polymers were developed, and a continuous adsorption system was constructed.

Academic Significance and Societal Importance of the Research Achievements

各種インフラや鉱山の開発に伴い,土壌・地下水の重金属汚染が生じる。従来の高エネルギー・高コストの処理技術に変わる原位置での低エネルギー・低環境負荷の革新的な重金属処理法が開発されれば,日本のみならず大規模なインフラ整備が急速に進みつつある世界各国においても有用な汚染土壌対策となりうる。本研究では,肥料に用いられる尿素,融雪剤に用いられる塩化カルシウム,および環境中に存在する微生物を用いて,常温で固化することが可能なバイオセメントにより重金属イオン汚染土壌の固化に成功した。さらに,タンパク質と天然多糖からなる新たな重金属イオン吸着剤を開発した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (47 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (20 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 20 results,  Open Access: 16 results) Presentation (22 results) (of which Int'l Joint Research: 10 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] University of Zambia(ザンビア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Zambia(ザンビア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Applications of chitosan in environmental remediation: A review2021

    • Author(s)
      Pal Preeti、Pal Anjali、Nakashima Kazunori、Yadav Brijesh Kumar
    • Journal Title

      Chemosphere

      Volume: 266 Pages: 128934-128934

    • DOI

      10.1016/j.chemosphere.2020.128934

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fabrication of silica on chitin in ambient conditions using silicatein fused with a chitin-binding domain2021

    • Author(s)
      Kasun Godigamuwa, Kazunori Nakashima, Sota Tsujitani, Satoru Kawasaki
    • Journal Title

      Bioprocess and Biosystems Engineering

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Wetting and Drying Cycles on the Durability of Bio-cemented Slope Soil2021

    • Author(s)
      Sivakumar Gowthaman, Kazunori Nakashima, Satoru Kawasaki
    • Journal Title

      International Journal of Environmental Science and Technology

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose2020

    • Author(s)
      TOGO Yuki、NAKASHIMA Kazunori、MWANDIRA Wilson、KAWASAKI Satoru
    • Journal Title

      Analytical Sciences

      Volume: 36 Issue: 4 Pages: 459-464

    • DOI

      10.2116/analsci.19P356

    • NAID

      130007830151

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2020-04-10
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine2020

    • Author(s)
      Mwandira Wilson、Nakashima Kazunori、Kawasaki Satoru、Arabelo Allison、Banda Kawawa、Nyambe Imasiku、Chirwa Meki、Ito Mayumi、Sato Tsutomu、Igarashi Toshifumi、Nakata Hokuto、Nakayama Shouta、Ishizuka Mayumi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 21189-21189

    • DOI

      10.1038/s41598-020-78187-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Influence of the Addition of Plant-Based Natural Fibers (Jute) on Biocemented Sand Using MICP Method2020

    • Author(s)
      Imran Md Al、Gowthaman Sivakumar、Nakashima Kazunori、Kawasaki Satoru
    • Journal Title

      Materials

      Volume: 13 Issue: 18 Pages: 4198-4198

    • DOI

      10.3390/ma13184198

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module2020

    • Author(s)
      Godigamuwa Kasun、Nakashima Kazunori、Okamoto Junnosuke、Kawasaki Satoru
    • Journal Title

      Biomacromolecules

      Volume: 21 Issue: 7 Pages: 2922-2928

    • DOI

      10.1021/acs.biomac.0c00730

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Freeze-thaw durability and shear responses of cemented slope soil treated by microbial induced carbonate precipitation2020

    • Author(s)
      Gowthaman Sivakumar、Nakashima Kazunori、Kawasaki Satoru
    • Journal Title

      Soils and Foundations

      Volume: 60 Issue: 4 Pages: 840-855

    • DOI

      10.1016/j.sandf.2020.05.012

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distribution and cementation characteristics of beachrocks along southern, southwestern and western coast of Sri Lanka2020

    • Author(s)
      Nayanthara Pahala Ge Nishadi、Dassanayake Anjula Buddhika Nayomi、Nakashima Kazunori、Kawasaki Satoru
    • Journal Title

      Journal of Sedimentary Environments

      Volume: 6 Issue: 1 Pages: 93-106

    • DOI

      10.1007/s43217-020-00036-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cellulose-metallothionein biosorbent for removal of Pb(II) and Zn(II) from polluted water2020

    • Author(s)
      Mwandira Wilson、Nakashima Kazunori、Togo Yuki、Sato Tsutomu、Kawasaki Satoru
    • Journal Title

      Chemosphere

      Volume: 246 Pages: 125733-125733

    • DOI

      10.1016/j.chemosphere.2019.125733

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Solidification of sand by Pb(II)-tolerant bacteria for capping mine waste to control metallic dust: Case of the abandoned Kabwe Mine, Zambia2019

    • Author(s)
      Mwandira Wilson、Nakashima Kazunori、Kawasaki Satoru、Ito Mayumi、Sato Tsutomu、Igarashi Toshifumi、Chirwa Meki、Banda Kawawa、Nyambe Imasiku、Nakayama Shouta、Nakata Hokuto、Ishizuka Mayumi
    • Journal Title

      Chemosphere

      Volume: 228 Pages: 17-25

    • DOI

      10.1016/j.chemosphere.2019.04.107

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chitosan enhances calcium carbonate precipitation and solidification mediated by bacteria2019

    • Author(s)
      Nawarathna Thiloththama Hiranya Kumari、Nakashima Kazunori、Kawasaki Satoru
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 133 Pages: 867-874

    • DOI

      10.1016/j.ijbiomac.2019.04.172

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region2019

    • Author(s)
      Sivakumar Gowthaman, Takashi Iki, Kazunori Nakashima, Koji Ebina, Satoru Kawasaki
    • Journal Title

      SN Applied Sciences

      Volume: 1 Issue: 11 Pages: 1480-1480

    • DOI

      10.1007/s42452-019-1508-y

    • NAID

      120006941694

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Factors Affecting the Urease Activity of Native Ureolytic Bacteria Isolated from Coastal Areas2019

    • Author(s)
      Md Al Imran, Kazunori Nakashima, Niki Evelpidou, and Satoru Kawasaki
    • Journal Title

      Geomechanics and Engineering

      Volume: 17(5) Pages: 421-427

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microbial induced slope surface stabilization using industrial-grade chemicals: A preliminary laboratory study2019

    • Author(s)
      Sivakumar Gowthaman, Shumpei Mitsuyama, Kazunori Nakashima, Masahiro Komatsu and Satoru Kawasaki
    • Journal Title

      International Journal of GEOMATE

      Volume: 17(60) Issue: 60 Pages: 110-116

    • DOI

      10.21660/2019.60.8150

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficacy of biocementation of lead mine waste from the Kabwe Mine site evaluated using Pararhodobacter sp.2019

    • Author(s)
      Mwandira Wilson、Nakashima Kazunori、Kawasaki Satoru、Ito Mayumi、Sato Tsutomu、Igarashi Toshifumi、Banda Kawawa、Chirwa Meki、Nyambe Imasiku、Nakayama Shouta、Ishizuka Mayumi
    • Journal Title

      Environmental Science and Pollution Research

      Volume: 印刷中 Issue: 15 Pages: 15653-15664

    • DOI

      10.1007/s11356-019-04984-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biogeotechnical Approach for Slope Soil Stabilization Using Locally Isolated Bacteria and Inexpensive Low-grade Chemicals: A Feasibility Study on Hokkaido Expressway Soil, Japan2018

    • Author(s)
      Sivakumar Gowthaman, Shumpei Mitsuyama, Kazunori Nakashima, Masahiro Komatsu and Satoru Kawasaki
    • Journal Title

      Soils and Foundations

      Volume: Articles in press Issue: 2 Pages: 1-16

    • DOI

      10.1016/j.sandf.2018.12.010

    • NAID

      120006820176

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of cationic polypeptide on CaCO3 crystallization and sand solidification by microbial-induced carbonate precipitation2018

    • Author(s)
      Thiloththama H. K. Nawarathna, Kazunori Nakashima, Masahiro Fujita, Momoko Takatsu and Satoru Kawasaki
    • Journal Title

      ACS Sustainable Chemistry and Engineering

      Volume: 6(8) Issue: 8 Pages: 10315-10322

    • DOI

      10.1021/acssuschemeng.8b01658

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Soil improvement using plant-derived urease-induced calcium carbonate precipitation2018

    • Author(s)
      R. A. N. Dilrukshi, Kazunori Nakashima and Satoru Kawasaki
    • Journal Title

      Soils and Foundations

      Volume: 58 Issue: 4 Pages: 894-910

    • DOI

      10.1016/j.sandf.2018.04.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A state-of-the-art review on soil reinforcement technology using natural plant fiber materials: Past findings, present trends and future directions2018

    • Author(s)
      Sivakumar Gowthaman, Kazunori Nakashima and Satoru Kawasaki
    • Journal Title

      Materials

      Volume: 11(4), 553 Issue: 4 Pages: 1-23

    • DOI

      10.3390/ma11040553

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 無機-有機接着タンパク質のデザインとバイオセメントへの応用2020

    • Author(s)
      川邉徹弥,中島一紀,Hiranya Nawarathna,川﨑了
    • Organizer
      生物工学若手研究者の集い第二回オンラインセミナー〈オンライン開催〉
    • Related Report
      2020 Annual Research Report
  • [Presentation] 炭酸カルシウムとバイオポリマーに結合する人工タンパク質の作製とバイオセメントへの影響2020

    • Author(s)
      川邉 徹弥,Nawarathna Hiranya,中島 一紀,川﨑 了
    • Organizer
      資源・素材 2020(仙台) 〈オンライン開催〉
    • Related Report
      2020 Annual Research Report
  • [Presentation] 機能性シリカ重合ペプチドを用いた無機材料の新たな作製法2020

    • Author(s)
      辻谷 颯太,中島 一紀,ゴディガムワ カスン,川﨑 了
    • Organizer
      資源・素材 2020(仙台) 〈オンライン開催〉
    • Related Report
      2020 Annual Research Report
  • [Presentation] Biological approach to form silica on chitin using a fusion silicatein2020

    • Author(s)
      ゴディガムワ カスン,中島 一紀,辻谷 颯太,川﨑 了
    • Organizer
      資源・素材 2020(仙台)
    • Related Report
      2020 Annual Research Report
  • [Presentation] シリカ重合酵素を用いた複合材料の作製2020

    • Author(s)
      辻谷 颯太,中島 一紀,川﨑 了
    • Organizer
      生物工学若手研究者の集い第三回オンラインセミナー〈オンライン開催〉
    • Related Report
      2020 Annual Research Report
  • [Presentation] Influence of Wet-Dry and Freeze-Thaw Cycles on the Physical and Mechanical Properties of MICP Treated Slope Soil2020

    • Author(s)
      S. Gowthaman, K. Nakashima, H. Nakamura, S. Kawasaki
    • Organizer
      54th U.S. Rock Mechanics/Geomechanics Symposium, ARMA (American Rock Mechanics Association)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation on the performance of MICP treated slope soil under acid rain environment2020

    • Author(s)
      Sivakumar Gowthaman, Arash Mohsenzadeh, Kazunori Nakashima, Hiromi Nakamura, Satoru Kawasaki
    • Organizer
      The Tenth International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Innovative Approach for Addressing Coastal Erosion Protection Using Microbial Induced Carbonate Precipitation2020

    • Author(s)
      Md Al Imran, Kazunori Nakashima, Niki Evelpidou, Satoru Kawasaki
    • Organizer
      European Geosciences Union General Assembly 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 尿素分解菌の細胞抽出液を用いた炭酸カルシウム形成と砂の固化2019

    • Author(s)
      川邉徹弥,中島一紀,Hiranya Nawarathna,川﨑了
    • Organizer
      2019年度資源・素材学会北海道支部春季講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バイオベース材料で構成された環境に優しい金属吸着材の開発2019

    • Author(s)
      東郷祐樹,中島一紀,川﨑了
    • Organizer
      日本生物工学会北日本支部2019札幌シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Zinc and Lead Biosorption by Oceanobacillus Profundus Kbz 3-2: a Bacterial Strain Resistant to Metals Isolated from Metal polluted Soils in Kabwe, Zambia2019

    • Author(s)
      Wilson Mwandira, Kazunori Nakashima, Satoru Kawasaki, Mayumi Ito, Tsutomu Sato, Toshifumi Igarashi, Meki Chirwa, Kawawa Banda, Imasiku Nyambe, Shouta Nakayama, Mayumi Ishizuka
    • Organizer
      The 14th Asian Congress on Biotechnology (ACB2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbially induced carbonate precipitation assisted by poly-L-lysine: ecofriendly approach inspired by nature.2019

    • Author(s)
      T.H.K. Nawarathna, K. Nakashima, S. Kawasaki
    • Organizer
      ACS Fall 2019 National Meeting & Exposition
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bio-based adsorbent composed of metal binding peptide and cellulose2019

    • Author(s)
      Y. Togo, K. Nakashima, W. Mwandira, S. Kawasaki
    • Organizer
      23rd International Biohydrometallurgy Symposium (IBS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炭酸塩の析出能力に着目した新たな微生物スクリーニング法2018

    • Author(s)
      菅原雄太,八重樫彩,中島一紀,川﨑了
    • Organizer
      資源・素材 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] バイオ-無機界面を考慮したタンパク質デザイン2018

    • Author(s)
      中島一紀
    • Organizer
      資源・素材2018
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 外膜表層に金属結合ペプチドを提示した微生物の無機固体表面に対する選択的な吸着特性2018

    • Author(s)
      岩田至叡, 中島一紀, 川﨑了
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] セルロースをベースとした金属イオン吸着剤の開発2018

    • Author(s)
      東郷祐樹, 岩田至叡, 岡本知之, 中島一紀, 川﨑了
    • Organizer
      平成30年度資源・素材学会北海道支部春季講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生物機能を用いた炭酸塩析出における炭酸脱水酵素の影響2018

    • Author(s)
      志賀敏英, 藤田賢大, 菅原雄太, 中島一紀, 川﨑了
    • Organizer
      平成30年度資源・素材学会北海道支部春季講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The Strength and Microstructure of lead-contaminated mine waste materials stabilized by Pararhodobacter sp.2018

    • Author(s)
      W. Mwandira, S. K. Nakashima, S. Kawasaki, M. Ito, T. Igarashi, T. Sato, M. Chirwa, K. E. Banda, I. Nyambe, M. Ishizuka
    • Organizer
      Bio-mediated and Bio-inspired Geotechnics (B2G-Atlanta 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Enzyme mediated CaCO3 crystallization in the presence of natural polysaccharides2018

    • Author(s)
      T.H.K. Nawarathna, Kazunori Nakashima, Satoru Kawasaki
    • Organizer
      14th International Chitin and Chitosan Conference (ICCC) & 12th Asia-Pacific Chitin and Chitosan Symposium (APCCS )
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biocementation using ureolytic bacteria: an eco-friendly alternative for treatment of mine waste in Kabwe Mine site2018

    • Author(s)
      W. Mwandira, K. Nakashima, S. Kawasaki, M. Ito, T. Sato, T. Igarashi, M. Chirwa, K. E. Banda, I. Nyambe, M. Ishizuka
    • Organizer
      2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bio-based cementation using urease-producing bacteria: sand solidification and kinetics2018

    • Author(s)
      K. Nakashima, M. Fujita, T. H. K. Nawarathna, S. Kawasaki
    • Organizer
      European Congress on Biotechnology (ECB2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Remarks]

    • URL

      https://bre.eng.hokudai.ac.jp/

    • Related Report
      2020 Annual Research Report
  • [Remarks]

    • URL

      https://labs.eng.hokudai.ac.jp/labo/bio-res/www/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 北海道大学・工学部・資源生物工学研究室HP

    • URL

      http://labs.eng.hokudai.ac.jp/labo/bio-res/www/index.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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