• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel water infrastructure able to control human norovirus prevalence

Research Project

Project/Area Number 18H03792
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 22:Civil engineering and related fields
Research InstitutionTohoku University

Principal Investigator

大村 達夫  東北大学, 未来科学技術共同研究センター, 名誉教授 (30111248)

Co-Investigator(Kenkyū-buntansha) 片山 浩之  東京大学, 大学院工学系研究科(工学部), 教授 (00302779)
渡部 徹  山形大学, 農学部, 教授 (10302192)
李 玉友  東北大学, 工学研究科, 教授 (30201106)
渡辺 幸三  愛媛大学, 沿岸環境科学研究センター, 教授 (80634435)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2019: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2018: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Keywordsノロウイルス / 消毒剤耐 / 次世代シーケンス / マウスノロウイルス / 進化 / 水インフラ / 消毒 / 除去 / 消毒剤耐性
Outline of Annual Research Achievements

昨年度にオンサイトのサニテーション施設において、糞便汚泥の消毒剤として使用されることがある消石灰(水酸化カルシウム)によるアルカリ処理に対して比較的抵抗性の高いマウスノロウイルス集団の取得に成功した。このようなアルカリ処理抵抗性の高いマウスノロウイルス集団をリバースジェネティクス法により人工的に創生することを試みた。アルカリ処理抵抗の高いマウスノロウイルス集団において特異的に見られた外殻タンパク質中のアミノ酸変異を人工的に合成した。さらに、得られた変異株(インフェクシャスクローン)について消毒剤感受性を評価し、どの遺伝子変異がどの程度消毒剤耐性に貢献していたかを調査した。その結果、創出したマウスノロウイルス粒子のアルカリ処理感受性は、当該アミノ酸変異を持たないマウスノロウイルス粒子に比べ、有意に高いアルカリ処理感受性を示した。このことから、今回マウスノロウイルス集団から見出されたアミノ酸変異がアルカリ処理抵抗性に関与していることが実験的に示された。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Report

(4 results)
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(23 results)

All 2022 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (15 results) (of which Int'l Joint Research: 12 results,  Invited: 2 results)

  • [Journal Article] Experimental Adaptation of Murine Norovirus to Calcium Hydroxide2022

    • Author(s)
      Oishi Wakana、Sato Mikiko、Kubota Kengo、Ishiyama Ryoka、Takai-Todaka Reiko、Haga Kei、Katayama Kazuhiko、Sano Daisuke
    • Journal Title

      Frontiers in Microbiology

      Volume: 13 Pages: 848439-848439

    • DOI

      10.3389/fmicb.2022.848439

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] EFFECT OF VIRAL GENOME PROPERTY ON THE EFFICIENCY OF VIABILITY (RT-)qPCR2020

    • Author(s)
      Vu Duc CANH, Hiroaki FURUMAI and Hiroyuki KATAYAMA
    • Journal Title

      土木学会論文集G(環境)

      Volume: 76 (7)

    • NAID

      130007999686

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Understanding Hurdles in the Implementation of Health Information System (HIS) Through an Institutional Framework: A Case of Bandung, West Java Province, Indonesia2020

    • Author(s)
      Faridah, L, F. Rinawan, N. Fauziah, W. Mayasari, A. Dwiartama, K. Watanabe
    • Journal Title

      bioRxiv

      Volume: 17(5) Pages: 1795-1795

    • DOI

      10.3390/ijerph17051795

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An Integrated mHealth App for Dengue Reporting and Mapping, Health Communication, and Behavior Modification: Development and Assessment of Mozzify2020

    • Author(s)
      Herbuela, V. R. D. M., T. Karita, M. Francisco, and K. Watanabe
    • Journal Title

      JMIR Formative Research

      Volume: 4

    • DOI

      10.2196/16424

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014-20162019

    • Author(s)
      Ito, E., J. Pu, T. Miura, S. Kazama, M. Nishiyama, H. Ito, Y. Konta, G. T. Nguyen, T. Omura, T. Watanabe
    • Journal Title

      Pathogens

      Volume: 8 Pages: 89-89

    • DOI

      10.3390/pathogens8030089

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Wolbachia Infection in Field-collected Mosquito Vector, Aedes aegypti in Metropolitan Manila, Philippines2019

    • Author(s)
      Carvajal,T. M., K. Hashimoto, R. K. Harnandika, D. M. Amalin and K. Watanabe
    • Journal Title

      Parasites & Vectors

      Volume: 12 (1) Pages: 361-361

    • DOI

      10.1186/s13071-019-3629-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Genetic Determinant of Norovirus for Susceptibility to Free Chlorine2019

    • Author(s)
      Andri Rachmadi, Masaaki Kitajima, Kozo Watanabe, Sakiko Yaegashi, Joeselle Serrana, Arata Nakamura, Toyoko Nakagomi, Osamu Nakagomi, Kazuhiko Kazuhiko Katayama, Satoshi Okabe, and Daisuke Sano
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 印刷中

    • DOI

      10.1128/aem.00244-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Weekly variations in Norovirus genogroup II genotypes in Japanese oysters2018

    • Author(s)
      Jian Pu, Takayuki Miura, Shinobu Kazama, Yoshimitsu Konta, Nabila Dhyan Azraini, Erika Ito, Hiroaki Ito, Tatsuo Omura, Toru Watanabe
    • Journal Title

      International Journal of Food Microbiology

      Volume: 284 Pages: 48-55

    • DOI

      10.1016/j.ijfoodmicro.2018.06.027

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] EFFECT OF VIRAL GENOME PROPERTY ON THE EFFICIENCY OF VIABILITY (RT-)qPCR2020

    • Author(s)
      Vu Duc CANH, Hiroaki FURUMAI and Hiroyuki KATAYAMA
    • Organizer
      第 57 回環境工学研究フォーラム
    • Related Report
      2020 Annual Research Report
  • [Presentation] Norovirus accumulation in wild estuarine bivalves and its relationship with gastroenteritis epidemics2019

    • Author(s)
      Erika Ito, Masateru Nishiyama, Jian Pu, Shinobu Kazama, Yoshimitsu Konta, Hiroyuki Kato, Tatsuo Omura, Toru Watanabe
    • Organizer
      Water and Environment Technology Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Norovirus accumulation in wild estuarine bivalves as an indicator of gastroenteritis epidemics: Comparison between oysters and clams (Nuttallia japonica)2019

    • Author(s)
      Erika Ito, Masateru Nishiyama, Jian Pu, Shinobu Kazama, Yoshimitsu Konta, Hiroyuki Kato, Tatsuo Omura, Toru Watanabe
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of personal hygiene on norovirus transmission within and among households2019

    • Author(s)
      Fuminari Miura, Toru Watanabe, Kozo Watanabe, Masateru Nishiyama, Erika Ito, Miina Yanagihara, Kensuke Fukushi
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contamination of cultured oysters with wild-type Rotavirus A and its relationship with gastroenteritis epidemic2019

    • Author(s)
      Erika Ito, Pu Jian, Takayuki Miura, Masateru Nishiyama, Toru Watanabe
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水中ノロウイルスのモニタリングと情報発信による感染性胃腸炎流行対策:仙台市における実証試験2019

    • Author(s)
      渡部徹,三浦郁修,西山正晃,伊藤絵里香,大住英俊,加藤裕之,大村達夫
    • Organizer
      第56回環境工学研究フォーラム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development and assessment of Mozzify app: an integrated mhealth for real-time Dengue reporting and mapping, health communication and behavior modification2019

    • Author(s)
      Herbuela, Von Ralph Dane M., Karita Tomonori, Francisco, Micanaldo, Watanabe Kozo
    • Organizer
      1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Designing and Developing a Nanobiosensor for the detection of important microbials in Ae. aegypti2019

    • Author(s)
      Carvajal Thadeus, Alocilja Evangeline, Divina Amalin, Watanabe Kozo
    • Organizer
      1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection of Wolbachia in Field-collected Dengue Vector Mosquito, Aedes aegypti in the Philippines2019

    • Author(s)
      Kozo Watanabe
    • Organizer
      Philippine Society for Microbiology 48th Annual Convention
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Contributing Factors to Childhood Stunting in Indonesia: Water Quality and Diarrhea2019

    • Author(s)
      Savira Ekawardhani, Hesti Lina Wiraswati, Lia Faridah, Kozo Watanabe
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring2019

    • Author(s)
      Joeselle Serrana, Yo Miyake, Maribet Gamboa, Kozo Watanabe
    • Organizer
      Pest Management Council of the Philippines 51st Anniversary and Annual Scientific Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水処理水放流先に自生する二枚貝中のノロウイルス量と周辺地域の感染症流行との関連2019

    • Author(s)
      伊藤絵里香,西山正晃,今田義光,大村達夫,渡部徹
    • Organizer
      平成30年度土木学会東北支部技術研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Seasonal variation of rotavirus concentration in cultured oysters in Japan2018

    • Author(s)
      Erika Ito, Hiroaki Ito, Jian Pu, Toru Watanabe
    • Organizer
      IWA World Water Congress & Exhibition 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Statistical forecasting of norovirus concentration in sewage as an indicator of future incidence2018

    • Author(s)
      Fuminari Miura, Toru Watanabe, Kozo Watanabe, Akihiko Hata, Kesuke Fukushi
    • Organizer
      IWA World Water Congress & Exhibition 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temporal variation of rotavirus in cultured oysters and its relation to gastroenteritis epidemics in Japan2018

    • Author(s)
      Erika Ito, Hiroaki Ito, Jian Pu, Masateru Nishiyama, Toru Watanabe
    • Organizer
      Water and Environment Technology Conference (WET2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2018-04-23   Modified: 2022-12-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi