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Full elucidation of sorting mechanisms in and around the Golgi apparatus by super-resolution live imaging

Research Project

Project/Area Number 18H05275
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Review Section Broad Section G
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

中野 明彦  国立研究開発法人理化学研究所, 光量子工学研究センター, 副センター長 (90142140)

Co-Investigator(Kenkyū-buntansha) 黒川 量雄  国立研究開発法人理化学研究所, 光量子工学研究センター, 専任研究員 (40333504)
戸島 拓郎  国立研究開発法人理化学研究所, 光量子工学研究センター, 上級研究員 (00373332)
植村 知博  お茶の水女子大学, 基幹研究院, 准教授 (90415092)
須田 恭之  筑波大学, 医学医療系, 助教 (10553844)
Project Period (FY) 2018-06-11 – 2023-03-31
Project Status Granted (Fiscal Year 2022)
Budget Amount *help
¥192,790,000 (Direct Cost: ¥148,300,000、Indirect Cost: ¥44,490,000)
Fiscal Year 2022: ¥33,280,000 (Direct Cost: ¥25,600,000、Indirect Cost: ¥7,680,000)
Fiscal Year 2021: ¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2020: ¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2019: ¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2018: ¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000)
Keywords膜交通 / ゴルジ体 / 選別輸送 / ライブイメージング
Outline of Annual Research Achievements

酵母細胞を用いた研究:①小胞体からゴルジ体への積荷の受け渡し。小胞体出口ERESにおける積荷選別の過程をライブイメージングで解析した(Sci. Adv. 誌発表)。また、ゴルジ体で積荷を受け取る初期区画GECCOで機能する分子の時空間的分離を解析した。②ゴルジ槽間の輸送に働くCOPIの変異により、一部のゴルジ体タンパク質が液胞に運ばれて分解されることを見出した。③ゴルジ体とTGNでのタンパク質選別の時空間的制御。ゴルジ体とTGNの境界に位置し、選別の鍵を握る分子をいくつか同定し、その挙動をライブイメージングで解析した。
植物細胞を用いた研究:①ゴルジ体初期区画GECCOによる小胞体からの積荷の受け取り。ゴルジ体の最もcis側の区画(GECCO)の研究をさらに進めた。②TGNでの複数経路の仕分けの時空間的制御。さまざまな被覆タンパク質およびアダブター複合体がTGNで集積・離散するダイナミクスを時空間的に解析した。AP-1とAP-4がそれぞれ分泌経路選別ゾーンと液胞経路選別ゾーンに集積することを示し、前者ではたらくクラスリン被覆小胞の挙動について詳細な4D解析を行った(Nat. Commun. 誌発表)。
動物細胞を用いた研究:①HeLa細胞を用い、TGNにおける積荷選別についてRUSHシステムと多色SCLIMによる4Dライブイメージングを開始した。②HeLa細胞およびショウジョウバエのS2細胞において、ゴルジ体層板とTGN/リサイクリングエンドソームが接着と解離を繰り返すことを見出し、またゴルジ体層板の集合にはArf GTPaseの活性化が関与していることを示した(J. Cell Sci. 誌発表、広島大学佐藤明子博士との共同研究)。③神経軸索におけるゴルジ体の役割。神経軸索成長円錐において、ゴルジ体の動態を観察した。積荷の挙動を観察できる新しいシステムの構築を進めている。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

酵母を用いた実験系で、タンパク質選別輸送の詳細な4Dライブイメージング解析を進め、ERESからゴルジ体cis槽へ、ゴルジ体内、ゴルジ体trans槽からTGNへ、そしてTGNから最終目的地へ、というプロセスで鍵を握る役者はほぼ把握できた。
植物と動物のゴルジ体に関する研究も順調に進んでいる。TGNについて、ゴルジ体以降の膜輸送過程の重要なハブとして機能している独立のオルガネラと考えられる証拠として、ゴルジ層板から離れて存在するTGNが、これまでリサイクリングエンドソームと認識されていた区画とほぼ同一のものであることを示し、動物と植物とでの驚くべき類似性を明らかにした。酵母での研究結果と統合して、膜交通の基礎概念を再び種を超えたパラダイムとして打ち立てる時が近づいている。
次世代SCLIM機、SCLIM2KとSCLIM2Mも順調に稼働し、高い時空間能を生かした成果を生みつつある。

Strategy for Future Research Activity

生きた細胞内の小胞輸送をありのままに目で見る技術が実現できている。予定した研究計画通り、異なる生物種のゴルジ体とその周辺オルガネラについて、徹底的な観察を行い、従来の生化学や遺伝学の実験結果に基づいて想像されていたモデルを一つ一つ検証していく。とくに、小胞体-ERES-cis Golgi-trans Golgi-TGNという流れの節目の選別過程で,重要な役割を果たす分子を相当数同定できた。今後、これらの役者がどのようにして異なる積荷を認識し、時空間的に分離させていくのかについて、さらなる研究を進めて行く予定である。

Assessment Rating
Interim Assessment Comments (Rating)

A: In light of the aim of introducing the research area into the research categories, the expected progress has been made in research.

Report

(7 results)
  • 2020 Abstract (Interim Assessment) ( PDF )   Annual Research Report   Interim Assessment (Comments) ( PDF )
  • 2019 Annual Research Report
  • 2018 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report

Research Products

(154 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (10 results) Journal Article (41 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 34 results,  Open Access: 23 results) Presentation (102 results) (of which Int'l Joint Research: 30 results,  Invited: 40 results) Remarks (1 results)

  • [Int'l Joint Research] University of Sevilla/Instituto de Biomedicina de Sevilla(スペイン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Institute of Biochem and Cell Biol/Institute of Nanotechnology/Istituto Nazionale Tumori Fondazione(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Geneva/University of Fribourg(スイス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Institute Curie(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Institute of Protein Biochemistry(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Sevilla(スペイン)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Max-Planck Inst. for Plant Breeding Res.(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Institute of Protein Biochemistry(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Sevilla(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Max Planck Inst for Plant Breeding Res(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3.2021

    • Author(s)
      Riccardo Rizzo, Domenico Russo, Kazuo Kurokawa, Alfredo Budillon, Franck Perez, Seetharaman Parashuraman, Yusuf A Hannun, Akihiko Nakano, Daniela Corda, Giovanni D’Angelo and Alberto Luini et al.,.
    • Journal Title

      EMBO J

      Volume: 40

    • DOI

      10.15252/embj.2020107238

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants2021

    • Author(s)
      Shimizu Yutaro、Takagi Junpei、Ito Emi、Ito Yoko、Ebine Kazuo、Komatsu Yamato、Goto Yumi、Sato Mayuko、Toyooka Kiminori、Ueda Takashi、Kurokawa Kazuo、Uemura Tomohiro、Nakano Akihiko
    • Journal Title

      Nature Communications

      Volume: 12 Pages: 1901-1901

    • DOI

      10.1038/s41467-021-22267-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 超解像ライブイメージング顕微鏡SCLIMの開発とSCLIMによるゴルジ体タンパク質輸送機構の解明2021

    • Author(s)
      黒川量雄,宮代大輔,中野明彦
    • Journal Title

      レーザー研究

      Volume: 49

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deletion of PIN4 Suppresses the Protein Transport Defects Caused by sec12-4 Mutation in Saccharomyces cerevisiae2020

    • Author(s)
      Murakami-Sekimata Akiko、Sekimata Masayuki、Sato Natsumi、Hayasaka Yuto、Nakano Akihiko
    • Journal Title

      Microbial Physiology

      Volume: 30 Pages: 25-35

    • DOI

      10.1159/000509633

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tricalbins are required for nonvesicular ceramide transport at ER-Golgi contacts and modulate lipid droplet biogenesis.2020

    • Author(s)
      Atsuko Ikeda, Philipp Schlarmann, Kazuo Kurokawa, Akihiko Nakano, Howard Riezman, and Kouichi Funato.
    • Journal Title

      iScience

      Volume: 23 Pages: 101603-101603

    • DOI

      10.1016/j.isci.2020.101603

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites.2020

    • Author(s)
      Sofia Rodriguez-Gallardo, Kazuo Kurokawa, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Atsuko Ikeda, Valeria Zoni, Auxiliadora Aguilera-Romero, Ana Maria Perez-Linero, Sergio Lopez, Miho Waga, Misako Araki, Miyako Nakano, Howard Riezman, Kouichi Funato, Stefano Vanni, Akihiko Nakano, Manuel Muñiz.
    • Journal Title

      Sci Adv.

      Volume: 6

    • DOI

      10.1126/sciadv.aba8237

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The liverwort oil body is formed by redirection of the secretory pathway2020

    • Author(s)
      Kanazawa T., Morinaka H., Ebine K., Shimada T.L., Ishida S., Minamino N., Yamaguchi K., Shigenobu S., Kohchi T., Nakano A., Ueda T.
    • Journal Title

      Nature Communications

      Volume: 11 Pages: 6152-6152

    • DOI

      10.1038/s41467-020-19978-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sec71 separates Golgi stacks in Drosophila S2 cells.2020

    • Author(s)
      Syara Fujii, Kazuo Kurokawa, Tatsuya Tago, Ryota Inaba, Arata Takiguchi, Akihiko Nakano, *Takunori Satoh, and *Akiko K Satoh.
    • Journal Title

      Journal of Cell Science

      Volume: 133

    • DOI

      10.1242/jcs.245571

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apoplastic Fluid Preparation from Arabidopsis thaliana Leaves Upon Interaction with a Nonadapted Powdery Mildew Pathogen2020

    • Author(s)
      Nakano Ryohei Thomas、Ishihama Nobuaki、Wang Yiming、Takagi Junpei、Uemura Tomohiro、Schulze-Lefert Paul、Nakagami Hirofumi
    • Journal Title

      Methods in Molecular Biology

      Volume: 2139 Pages: 79-88

    • DOI

      10.1007/978-1-0716-0528-8_6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution.2020

    • Author(s)
      Kazuo Kurokawa and Akihiko Nakano
    • Journal Title

      BIO-PROTOCOL

      Volume: 10

    • DOI

      10.21769/bioprotoc.3732

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells2020

    • Author(s)
      Fujii Syara、Kurokawa Kazuo、Inaba Ryota、Hiramatsu Naoki、Tago Tatsuya、Nakamura Yuri、Nakano Akihiko、Satoh Takunori、Satoh Akiko K.
    • Journal Title

      Journal of Cell Science

      Volume: 133 Pages: 236935-236935

    • DOI

      10.1242/jcs.236935

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three YXXL Sequences of a Bovine Leukemia Virus Transmembrane Protein are Independently Required for Fusion Activity by Controlling Expression on the Cell Membrane2019

    • Author(s)
      Matsuura Ryosuke、Inabe Kazunori、Otsuki Hiroyuki、Kurokawa Kazuo、Dohmae Naoshi、Aida Yoko
    • Journal Title

      Viruses

      Volume: 11 Pages: 1140-1140

    • DOI

      10.3390/v11121140

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HIGH STEROL ESTER 1 is a key factor in plant sterol homeostasis2019

    • Author(s)
      Shimada Takashi L.、Shimada Tomoo、Okazaki Yozo、Higashi Yasuhiro、Saito Kazuki、Kuwata Keiko、Oyama Kaori、Kato Misako、Ueda Haruko、Nakano Akihiko、Ueda Takashi、Takano Yoshitaka、Hara-Nishimura Ikuko
    • Journal Title

      Nature Plants

      Volume: 5 Pages: 1154-1166

    • DOI

      10.1038/s41477-019-0537-2

    • NAID

      120006773162

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Atg8 in the regulation of vacuolar membrane invagination2019

    • Author(s)
      Ishii Ayane、Kurokawa Kazuo、Hotta Miyuu、Yoshizaki Suzuka、Kurita Maki、Koyama Aya、Nakano Akihiko、Kimura Yoko
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 14828-14828

    • DOI

      10.1038/s41598-019-51254-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatiotemporal dissection of the trans-Golgi network in budding yeast2019

    • Author(s)
      Takuro Tojima, Yasuyuki Suda, Midori Ishii, Kazuo Kurokawa, Akihiko Nakano
    • Journal Title

      Journal of Cell Science

      Volume: 132 Pages: 231159-231159

    • DOI

      10.1242/jcs.231159

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] COPII proteins exhibit distinct subdomains within each ER exit site for executing their functions2019

    • Author(s)
      Maeda Miharu、Kurokawa Kazuo、Katada Toshiaki、Nakano Akihiko、Saito Kota
    • Journal Title

      Sci Rep.

      Volume: 9 Pages: 7346-7346

    • DOI

      10.1038/s41598-019-43813-3

    • NAID

      120007169371

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enrichment of Phosphatidylinositol 4,5-Bisphosphate in the Extra-Invasive Hyphal Membrane Promotes Colletotrichum Infection of Arabidopsis thaliana2019

    • Author(s)
      Shimada Takashi L、Betsuyaku Shigeyuki、Inada Noriko、Ebine Kazuo、Fujimoto Masaru、Uemura Tomohiro、Takano Yoshitaka、Fukuda Hiroo、Nakano Akihiko、Ueda Takashi
    • Journal Title

      Plant Cell Physiol.

      Volume: 60 Pages: 1514-1524

    • DOI

      10.1093/pcp/pcz058

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network2019

    • Author(s)
      Zhao Shen-Bao、Suda Yasuyuki、Nakanishi Hideki、Wang Ning、Yoko-o Takehiko、Gao Xiao-Dong、Fujita Morihisa
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 1863 Pages: 1147-1157

    • DOI

      10.1016/j.bbagen.2019.04.009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transient activity of the florigen complex during the floral transition in Arabidopsis thaliana2019

    • Author(s)
      Abe Mitsutomo、Kosaka Shingo、Shibuta Mio、Nagata Kenji、Uemura Tomohiro、Nakano Akihiko、Kaya Hidetaka
    • Journal Title

      Development

      Volume: 146

    • DOI

      10.1242/dev.171504

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization of secretory cargo transport within the Golgi apparatus2019

    • Author(s)
      Kurokawa K, Osakada H, Kojidani T, Waga M, Suda Y, Asakawa H, Haraguchi T, Nakano A.
    • Journal Title

      Journal of Cell Biology

      Volume: - Pages: 1602-1618

    • DOI

      10.1083/jcb.201807194

    • NAID

      120007133506

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Editorial overview: Membrane trafficking2019

    • Author(s)
      Cullen Peter J、Nakano Akihiko
    • Journal Title

      Current Opinion in Cell Biology

      Volume: 59

    • DOI

      10.1016/j.ceb.2019.06.005

    • Related Report
      2019 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis2019

    • Author(s)
      Uemura Tomohiro、Nakano Ryohei Thomas、Takagi Junpei、Wang Yiming、Kramer Katharina、Finkemeier Iris、Nakagami Hirofumi、Tsuda Kenichi、Ueda Takashi、Schulze-Lefert Paul、Nakano Akihiko
    • Journal Title

      Plant Phys.

      Volume: 179 Pages: 519-532

    • DOI

      10.1104/pp.18.01228

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enrichment of phosphatidylinositol 4,5-bisphosphate in the extra-invasive hyphal membrane promotes Colletotrichum infection of Arabidopsis thaliana2019

    • Author(s)
      Takashi L. Shimada, Shigeyuki Betsuyaku, Noriko Inada, Kazuo Ebine, Masaru Fujimoto, Tomohiro Uemura, Yoshitaka Takano, Hiroo Fukuda, Akihiko Nakano, and Takashi Ueda
    • Journal Title

      Plant and Cell Physiology

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organelle zones2019

    • Author(s)
      Nakano Akihiko、von Blume Julia
    • Journal Title

      Molecular Biology of the Cell

      Volume: 30 Pages: 731-731

    • DOI

      10.1091/mbc.e18-12-0818

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 生化学者の地動説2019

    • Author(s)
      中野 明彦
    • Journal Title

      生化学

      Volume: 91 Issue: 1 Pages: 1-1

    • DOI

      10.14952/SEIKAGAKU.2019.910001

    • ISSN
      0037-1017
    • Related Report
      2018 Annual Research Report
  • [Journal Article] トランスゴルジ網2019

    • Author(s)
      戸島拓郎,中野明彦
    • Journal Title

      Organelle Zone On Line News Letter

      Volume: 2 Pages: 21-21

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Brassinosteroids regulate vacuolar morphology in root meristem cells of Arabidopsis thaliana.2018

    • Author(s)
      Yamagami A, Saito C, Sakuta M, Shinozaki K, Osada H, Nakano A, Asami T, Nakano T
    • Journal Title

      Plant Signaling & Behavior

      Volume: 印刷中

    • DOI

      10.1080/15592324.2017.1417722

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RAB GTPases in the Basal Land Plant Marchantia polymorpha2018

    • Author(s)
      Minamino Naoki、Kanazawa Takehiko、Era Atsuko、Ebine Kazuo、Nakano Akihiko、Ueda Takashi
    • Journal Title

      Plant Cell Phys

      Volume: in press Pages: 845-856

    • DOI

      10.1093/pcp/pcy027

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Integration of two RAB5 groups during endosomal transport in plants2018

    • Author(s)
      Ito Emi、Ebine Kazuo、Choi Seung-won、Ichinose Sakura、Uemura Tomohiro、Nakano Akihiko、Ueda Takashi
    • Journal Title

      eLife

      Volume: 7

    • DOI

      10.7554/elife.34064

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detailed Analysis of the Interaction of Yeast COG Complex2018

    • Author(s)
      Ishii Midori、Lupashin Vladimir V.、Nakano Akihiko
    • Journal Title

      Cell Struct. Funct.

      Volume: 43 Issue: 2 Pages: 119-127

    • DOI

      10.1247/csf.18014

    • NAID

      130007366858

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional Diversity of Class XI Myosins in Arabidopsis thaliana2018

    • Author(s)
      Haraguchi T., Ito K., Duan Z, Rula S, Takahashi K., Shibuya Y., Hagino N., Miyatake Y., Nakano A., Nakano A., and Tominaga M
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2268-2277

    • DOI

      10.1093/pcp/pcy147

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synaptotagmin-associated endoplasmic reticulum-plasma membrane contact sites are localized to immobile ER tubules2018

    • Author(s)
      K. Ishikawa, Kentaro Tamura, H. Ueda, Y. Ito, A. Nakano, I. Hara-Nishimura, and T. Shimada
    • Journal Title

      Plant Physiology

      Volume: 178 Pages: 641-653

    • DOI

      10.1104/pp.18.00498

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases2018

    • Author(s)
      Muro Keita、Matsuura-Tokita Kumi、Tsukamoto Ryoko、Kanaoka Masahiro M.、Ebine Kazuo、Higashiyama Tetsuya、Nakano Akihiko、Ueda Takashi
    • Journal Title

      Commun. Biol.

      Volume: 1 Pages: 152-152

    • DOI

      10.1038/s42003-018-0158-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana2018

    • Author(s)
      Takagi Junpei、Uemura Tomohiro
    • Journal Title

      Methods in Molecular Biology

      Volume: 1789 Pages: 155-165

    • DOI

      10.1007/978-1-4939-7856-4_12

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of LRG1 expression by RNA‐binding protein Puf5 in the budding yeast cell wall integrity pathway2018

    • Author(s)
      Viet Nguyen Thi Minh、Duy Duong Long、Saito Kazuhiro、Irie Kaoru、Suda Yasuyuki、Mizuno Tomoaki、Irie Kenji
    • Journal Title

      Genes Cells.

      Volume: 23 Pages: 988-997

    • DOI

      10.1111/gtc.12646

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The ER exit sites are specialized ER zones for the transport of cargo proteins from the ER to the Golgi apparatus2018

    • Author(s)
      Kurokawa Kazuo、Nakano Akihiko
    • Journal Title

      J. Biochem.

      Volume: 165 Pages: 109-114

    • DOI

      10.1093/jb/mvy080

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overturning paradigms of membrane traffic by yeast live imaging2018

    • Author(s)
      Nakano Akihiko
    • Journal Title

      PL. MORPH.

      Volume: 30 Issue: 1 Pages: 3-4

    • DOI

      10.5685/plmorphol.30.3

    • NAID

      130007622404

    • ISSN
      0918-9726, 1884-4154
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 小胞体からゴルジ体への分子輸送機構2018

    • Author(s)
      黒川量雄,中野明彦
    • Journal Title

      細胞

      Volume: 50 Pages: 12-15

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 特集「細胞高次機能をつかさどるオルガネラコミュニケーション」によせて2018

    • Author(s)
      中野明彦
    • Journal Title

      生体の科学

      Volume: 69 Pages: 526-526

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 小胞体からゴルジ体へのタンパク質選別輸送2018

    • Author(s)
      黒川量雄
    • Journal Title

      生体の科学

      Volume: 69 Pages: 532-535

    • Related Report
      2018 Annual Research Report
  • [Journal Article] SCLIM-超解像共焦点ライブイメージング顕微鏡2018

    • Author(s)
      中野明彦,宮代大輔
    • Journal Title

      実験医学

      Volume: 36 Pages: 3450-3452

    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物TGNにおけるタンパク質輸送選別ゾーンの可視化2021

    • Author(s)
      植村知博
    • Organizer
      第22回植物オルガネラワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 出芽酵母におけるトランスゴルジ網の槽成熟ダイナミクス2021

    • Author(s)
      戸島拓郎
    • Organizer
      第22回植物オルガネラワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 植物 RAB5 のエフェクターを介した機能実行メカニズムの解析2021

    • Author(s)
      伊藤瑛海,崔勝媛,竹内一貴,海老根一生,中野明彦,上田貴志,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Syntaxin6 N 末端領域を有する植物固有タンパク質の細胞内局在の解析2021

    • Author(s)
      長尾レイナ,清水優太朗,藤倉崇紘,伊藤瑛海,由良敬,中野明彦,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ Rab6/RABH1 の機能解析2021

    • Author(s)
      大堀智博,伊藤瑛海,中野明彦,上田貴志,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 病原菌応答における植物分泌システムの役割2021

    • Author(s)
      遠藤彩瑛,伊藤瑛海,中野明彦,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ VAMP714 の細胞内局在の解析2021

    • Author(s)
      江口倫子,遠藤彩瑛,伊藤瑛海,中野明彦,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] TGN-液胞間の輸送が制御する塩ストレス応答2021

    • Author(s)
      井上柚紀,清水優太朗,伊藤瑛海,中野明彦,植村知博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 膜交通制御因子 SYP61 による塩ストレス応答制御におけるユビキチンシグナルの関与2021

    • Author(s)
      新井麻由,長谷川陽子,Yongming Luo,椋田航生,植村知博,高木純平,山口淳二,佐藤長緒
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ユビキチンシグナルと膜交通制御を介した植物の C/N 栄養応答の解析2021

    • Author(s)
      長谷川陽子,羅永茗,椋田航生,新井麻由,植村知博,Yohann Boutte,中野明彦,山口淳二,佐藤長緒
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Golgi and next-door neighbors - a comparative view of yeast, plant and animal cells2020

    • Author(s)
      Akihiko Nakano
    • Organizer
      CELL BIO virtual 2020 (Online ASCB | EMBO Meeting)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 膜交通の新パラダイムを構築しよう2020

    • Author(s)
      中野明彦
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 4Dライブイメージングによる小胞体,ゴルジ体の積荷タンパク質輸送機構の解明2020

    • Author(s)
      黒川量雄
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 神経成長円錐における膜交通ダイナミクス2020

    • Author(s)
      戸島拓郎
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 細胞内膜交通の超高時空間分解能ライブイメージング2020

    • Author(s)
      中野明彦
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ミトコンドリア- 小胞体間結合因子ERMES 複合体クラスターの解離がER ストレス軽減に寄与する2020

    • Author(s)
      柿元百合子,小島理恵子,新名真夏,黒川量雄,中野明彦,遠藤斗志也,田村康
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 小胞体における積荷タンパク質選別輸送機構2020

    • Author(s)
      黒川量雄,Sofia Gallardo,Manuel Muniz,中野明彦
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 出芽酵母の胞子形成におけるVps13アダプタータンパク質Spo71の解析2020

    • Author(s)
      藤枝祐二,棟重賢治,中村毅,須田恭之,舘川宏之
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 膜交通関連オルガネラの減数分裂に伴う再構成2020

    • Author(s)
      須田恭之,舘川宏之,黒川量雄,中野明彦,入江賢児
    • Organizer
      第72回日本細胞生物学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脂質輸送タンパク質Vps13の出芽酵母前胞子膜形成における役割2020

    • Author(s)
      藤枝祐二,棟重賢治,中村毅,中西秀樹,須田恭之,舘川宏之
    • Organizer
      酵母遺伝学フォーラム第53回研究報告会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Plant-unique RAB5 effector 3の細胞内動態解析2020

    • Author(s)
      伊藤瑛海,崔勝媛,竹内一貴,海老根一生,中野明彦,上田貴志,植村知博
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物のC/N栄養応答におけるユビキチンシグナルと膜交通制御のシロイヌナズナを用いた解析2020

    • Author(s)
      長谷川陽子,羅永茗,椋田航生,新井麻由,植村知博,Yohann Boutte,中野明彦,佐藤長緒,山口 淳二
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Syntaxin6 N末端領域を有する植物固有タンパク質の細胞内局在の解析2020

    • Author(s)
      長尾レイナ,清水優太朗,藤倉崇紘,伊藤瑛海,由良敬,中野明彦,植村知博
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナVAMP714の細胞内局在の解析2020

    • Author(s)
      江口倫子,遠藤彩瑛,伊藤瑛海,中野明彦,植村知博
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物TGN が制御するうどんこ病菌に対する抵抗性2020

    • Author(s)
      植村知博
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 膜局在型ユビキチンリガーゼATL31とSNAREタンパク質SYP61 を介したC/N 栄養応答制御機構の解析2020

    • Author(s)
      長谷川陽子,藤巻あかり,羅永茗,椋田航生,新井麻由,植村知博,Boutt_ Yohann,中野明彦,佐藤長緒,山口淳二
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] トランスゴルジ網の液胞輸送ゾーンと多胞体/液胞前区画の時空間動態の研究2020

    • Author(s)
      清水優太朗,高木純平,伊藤容子,小松大和,海老根一生,上田貴志,黒川量雄,植村知博,中野明彦
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The challenge to visualize vesicular trafficking in living cells by high-speed and super-resolution live imaging microscopy _The dream has come true2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      Full-global kickoff symposium, Serendipity Lab.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of the membrane traffic in budding yeast2019

    • Author(s)
      Yasuyuki Suda
    • Organizer
      International graduate symposium on industrial biotechnology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of the Golgi and its neighbors visualized by high-speed and super-resolution live imaging SCLIM2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      Symposium “Structure and Function of the Golgi,” 92nd Annual Meeting of the Japanese Biochemical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] State-of-the-art live cell imaging at high-speed and super-resolution -- Dream to see real vesicular trafficking has come true2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      University of Oslo Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] State-of-the-art live cell imaging at high-speed and super-resolution -- Dream to see real vesicular trafficking has come true2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      University of Bergen Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The identity of the trans-Golgi network revealed by super-resolution live imaging2019

    • Author(s)
      Takuro Tojima, Yasuyuki Suda, Midori Ishii, Kazuo Kurokawa, and Akihiko Nakano
    • Organizer
      Gordon Research Conference on Molecular Membrane Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of Atg8 in the regulation of vacuolar membrane homeostasis2019

    • Author(s)
      Ayane Ishii, Kazuo Kurokawa, Miyuu Hotta, Suzuka Yoshizaki, Maki Kurita, Aya Koyama, Akihiko Nakano, and Yoko Kimura
    • Organizer
      Gordon Research Conference on Molecular Membrane Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of transmembrane cargo entry into the ERES in S. cerevisiae2019

    • Author(s)
      Kazuo Kurokawa and Akihiko Nakano
    • Organizer
      Gordon Research Conference on Molecular Membrane Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed and super-resolution live imaging to understand the true world in a living cell2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      University of Michigan Seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Extremely dynamic behaviors of vesicles and zones in an around the Golgi as visualized by high-speed super-resolution live imaging SCLIM2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      IAS Symposium “Biogenesis of Intracellular and Extracellular Vesicles”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] State-of-the-art live cell imaging at high-speed and super-resolution -- Dream to see real vesicular trafficking has come true2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      Joint Symposium “Extreme imaging to explore the boundaries between cell biology and protein science,” Joint Annual Meeting of 71st JSCB and 19th PSSJ
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of membrane traffic in the neuronal growth cone.2019

    • Author(s)
      Takuro Tojima
    • Organizer
      RIKEN symposium: Cutting edge of membrane traffic
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of cargo transport from the ER to the Golgi and within the Golgi2019

    • Author(s)
      Kazuo Kurokawa
    • Organizer
      RIKEN symposium: Cutting edge of membrane traffic
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of protein sorting zones in and around the Golgi apparatus as visualized by high-speed super-resolution live imaging SCLIM2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      International Symposium “Organelle zones: opening a new ear of cell biology”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of protein sorting zones within and around the Golgi apparatus as visualized by high-speed super-resolution live imaging SCLIM2019

    • Author(s)
      Akihiko Nakano
    • Organizer
      SFB1190 Minisymposium “Organelle Zones Meet Compartmental Gates and Contact Sites”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of membrane traffic in the neuronal growth cone revealed by super-resolution live imaging2019

    • Author(s)
      戸島拓郎
    • Organizer
      理研RAPシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 小胞体におけるGPIアンカー型タンパク質選別の可視化2019

    • Author(s)
      黒川量雄,Sof_a Rodr_guez Gallardo,Manuel Mu_iz,中野明彦
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゴルジ体からトランスゴルジ網への槽成熟ダイナミクス2019

    • Author(s)
      戸島拓郎
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 神経成長円錐における膜交通の可視化解析2019

    • Author(s)
      戸島拓郎
    • Organizer
      第3回オルガネラゾーン研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物TGNにおけるタンパク質輸送選別ゾーンの可視化2019

    • Author(s)
      植村知博
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 液胞膜の膜融合装置の解析2019

    • Author(s)
      海老根一生,竹元廣大,齊藤知恵子,植村知博,中野明彦,上田貴志
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 4Dイメージングによる小胞体-ゴルジ体間,ゴルジ体内の蛋白質輸送機構2019

    • Author(s)
      黒川量雄
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 植物TGNにおけるタンパク質輸送選別ゾーンの可視化2019

    • Author(s)
      植村知博
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 小胞体における積荷タンパク質選別輸送ゾーンの解析2019

    • Author(s)
      黒川量雄,Sof_a Rodr_guez Gallardo,Manuel Mu_iz,中野明彦
    • Organizer
      酵母遺伝学フォーラム第52回研究報告会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 出芽酵母減数分裂における膜交通関連オルガネラの再構成2019

    • Author(s)
      須田恭之,舘川宏之,中野明彦,入江賢児
    • Organizer
      酵母遺伝学フォーラム第52回研究報告会
    • Related Report
      2019 Annual Research Report
  • [Presentation] AP-4複合体を介したTGNからMVBへの輸送機構の解明に向けて2019

    • Author(s)
      清水優太朗,高木純平,伊藤容子,小松大和,海老根一生,上田貴志,黒川量雄,植村知博,中野明彦
    • Organizer
      第8回植物エンドメンブレンミーティング
    • Related Report
      2019 Annual Research Report
  • [Presentation] 成熟するゴルジ槽内のゾーン形成と積荷タンパク質輸送の可視化2019

    • Author(s)
      黒川量雄
    • Organizer
      第38回日本糖質学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナのトランスゴルジ網における積荷タンパク質選別ゾーンの時空間的解析2019

    • Author(s)
      清水優太朗,高木純平,伊藤容子,小松大和,海老根一生,上田貴志,黒川量雄,植村知博,中野明彦
    • Organizer
      第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ゴルジ体内の積荷タンパク質輸送の可視化2019

    • Author(s)
      黒川量雄,中野明彦
    • Organizer
      第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] トランスゴルジ網形成の時空間ダイナミクス2019

    • Author(s)
      戸島拓郎,須田恭之,石井みどり,黒川量雄,中野明彦
    • Organizer
      第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 出芽酵母減数分裂における膜交通関連オルガネラの再構成2019

    • Author(s)
      須田恭之,舘川宏之,中野明彦,入江賢児
    • Organizer
      第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 究極のライブセルイメージングでパラダイムを覆す2019

    • Author(s)
      中野明彦
    • Organizer
      ERATO学術セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 高速超解像ライブイメージングではじめて見えてきた膜交通の真の姿2019

    • Author(s)
      中野明彦
    • Organizer
      大阪大学免疫細胞学セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナのトランスゴルジ網における積荷タンパク質選別ゾーンの研究2019

    • Author(s)
      清水優太朗、_木純平、伊藤容子、小松大和、海老根一生、上田貴志、黒川量雄、植村知博、中野明彦
    • Organizer
      オルガネラ・ゾーン若手の会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 膜交通制御因子SNAREタンパク質のユビキチン化に関する生化学的解析2019

    • Author(s)
      藤巻あかり、長谷川陽子、保月彰太、植村知博、中野明彦、佐藤長緒、山口淳二
    • Organizer
      日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物のC/N栄養応答における膜交通制御の解析2019

    • Author(s)
      長谷川陽子、藤巻あかり、椋田航生、植村知博、Yohann Boutt_、中野明彦、佐藤長緒、山口淳二
    • Organizer
      日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小胞体タンパク質HIGH STEROL ESTER 1によるステロール恒常性維持機構2019

    • Author(s)
      島田貴士,嶋田知生,岡咲洋三,東泰弘,斉藤和季,桑田啓子,小山香梨,加藤美砂子,高野義孝,上田貴志,中野明彦,上田晴子,西村いくこ
    • Organizer
      日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of RAB5 effectors containing PH domain REAP2 and REAP3 in Arabidopsis2019

    • Author(s)
      Seung-won Choi, Kazuo Ebine, Naoya Kato, Takafumi Ishihara, Chie Suzuki, Yuki Sugiyama, Yumiko Tanaka, Takashi Ueda, Akihiko Nakano, Emi Ito
    • Organizer
      日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] High-speed and super-resolution live imaging to understand mechanisms of protein trafficking from the ER and through the Golgi apparatus2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      Oxford University Department of Biochemistry Seminar
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] High-speed super-resolution live imaging of membrane trafficking2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      The Company of Biologists Workshop “Cellular gateways: expanding the role of endocytosis in plant development”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed and super-resolution live imaging of membrane trafficking2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      University of Sevilla Seminar
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Spatiotemporal dissection of trafficking events in and around the Golgi2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of secretory cargo transport within the Golgi apparatus in living yeast cells2018

    • Author(s)
      Kazuo Kurokawa, Yasuyuki Suda, and Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] GECCO: the Golgi entry and scaffold compartment that is formed independently of COPII in plant cells2018

    • Author(s)
      Yoko Ito, Tomohiro Uemura, Akihiko Nakano, and Yohann Boutt_
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamics of coat and adaptor proteins at the trans-Golgi network revealed by super-resolution live imaging2018

    • Author(s)
      Takuro Tojima, Yasuyuki Suda, Midori Ishii, Kazuo Kurokawa, and Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamics of protein sorting zones on the trans-Golgi network (TGN) revealed by super-resolution confocal live imaging microscopy (SCLIM) in plant2018

    • Author(s)
      Tomohiro Uemura, Yutaro Shimizu, and Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Post-Golgi vesicle tethering and fusion for de novo membrane formation during sporulation in budding yeast2018

    • Author(s)
      Yasuyuki Suda, Hiroyuki Tachikawa, Kenji Irie, and Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of high-speed and super-resolution microscopy for visualizing membrane traffic2018

    • Author(s)
      Daisuke Miyashiro and Akihiko Nakano
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] GOLPH3 promotes oncogenesis by controlling the intra-Golgi recycling of glycosphingolipid synthases2018

    • Author(s)
      R Rizzo, D Russo, K Kurokawa, ,,,, T Yamaji, K Hanada, A Budillon, S Parashuraman, F Perez, L M. Obeid, A Nakano, Y A Hannun, G D’Angelo, and A Luini
    • Organizer
      The 2018 FEBS Golgi meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed and super-resolution live imaging of membrane trafficking2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      University of Bordeaux Seminar
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Organelle zones: a new concept emerging from cutting-edge live imaging microscopy2018

    • Author(s)
      Akihiko Nakano
    • Organizer
      ASCB | EMBO 2018 meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 4D imaging of membrane traffic in the growth cone2018

    • Author(s)
      Takuro Tojima and Akihiko Nakano
    • Organizer
      Cold Spring Harbor Asia conference on Latest Advances in Development & Function of Neuronal Circuits
    • Related Report
      2018 Annual Research Report
  • [Presentation] Probing secretion in plant cells: Echidna and associates2018

    • Author(s)
      Anirban Baral, Delphine Gendre, Kazuo Ebine, Yohann Boutte, Tomohiro Uemura, Takashi Ueda, and Rishikesh P Bhalerao
    • Organizer
      21th European Network for Plant Endomembrane Research meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 4D live cell imaging unveils cargo sorting zones of trans-Golgi network in Arabidopsis2018

    • Author(s)
      Yutaro Shimizu, Junpei Takagi, Yoko Ito, Yamato Komatsu, Kazuo Ebine, Takashi Ueda, Kazuo Kurokawa, Tomohiro Uemura, and Akihiko Nakano
    • Organizer
      21th European Network for Plant Endomembrane Research meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of regulation of plant nutrition stress response by a ubiquitin ligase ATL31 through modification of membrane traffic2018

    • Author(s)
      Yoko Hasegawa, Thais Huarancca Reyes, Tomohiro Uemura, Akari Fujimaki, Shota Hozuki, Anirban Baral, Rishikesh P. Bhalerao, Takeo Sato, Akihiko Nakano, and Junji Yamaguchi
    • Organizer
      21th European Network for Plant Endomembrane Research meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 4D live cell imaging unveils functional zones of trans-Golgi network in Arabidopsis2018

    • Author(s)
      Yutaro Shimizu, Junpei Takagi, Yoko Ito, Yamato Komatsu, Kazuo Ebine, Takashi Ueda, Kazuo Kurokawa, Tomohiro Uemura, and Akihiko Nakano
    • Organizer
      RIKEN Summer School 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Live imaging of the Golgi apparatus in Saccharomyces cerevisiae2018

    • Author(s)
      Yasuyuki Suda
    • Organizer
      Mini-symposium on membrane trafficking and remodeling
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] オルガネラゾーン研究を究める高速超解像ライブイメージング2018

    • Author(s)
      中野明彦
    • Organizer
      日本生化学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 小胞体における積荷タンパク質選別機構2018

    • Author(s)
      黒川量雄, Manuel Mu_iz, 中野明彦
    • Organizer
      日本生化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ステロールエステル蓄積オルガネラ・SEボディによる植物ステロール恒常性維持機構の解明2018

    • Author(s)
      島田貴士,嶋田知生,岡咲洋三,斉藤和季中野明彦,上田貴志,高野義孝,西村いくこ
    • Organizer
      日本生化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゼニゴケの精子形成に関わる膜交通因子の解析2018

    • Author(s)
      南野尚紀,金澤建彦,法月拓也,石田咲子,西浜竜一,河内孝之,中野明彦,上田貴志
    • Organizer
      日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 4Dイメージングで迫る積荷タンパク質のゴルジ体内輸送機構2018

    • Author(s)
      黒川量雄、宮代大輔、中野明彦
    • Organizer
      バイオイメージング学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] サブ波長フォトニクス研究が目指すもの ~高速超解像ライブイメージングの究極は?~2018

    • Author(s)
      中野明彦
    • Organizer
      RAPシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 小胞体における積荷タンパク質選別輸送ゾーンの解析2018

    • Author(s)
      黒川量雄, Manuel Mu_iz, 中野明彦
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 出芽酵母前胞子膜伸長におけるPI4Pおよびオルガネラ接触部位の役割の解析2018

    • Author(s)
      中村 毅, 棟重賢治, 須田恭之, 舘川宏之
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Atg8による熱ストレス時の液胞膜陥入形成の制御2018

    • Author(s)
      石井彩音、黒川量雄、中野明彦、木村洋子
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 4D imaging of membrane traffic in the neuronal growth cone2018

    • Author(s)
      Takuro Tojima and Akihiko Nakano
    • Organizer
      日本細胞生物学会、日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Visualization of GPI-anchored proteins sorting in the ER2018

    • Author(s)
      Kazuo Kurokawa, Atsuko Ikeda, Koichi Funato, Manuel Mu_iz, Akihiko Nakano
    • Organizer
      日本細胞生物学会、日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cytoplasmic deadenylases Ccr4 is required for translational repression of Puf5 mRNA targets in the stationary phase in Saccharomyces cerevisiae2018

    • Author(s)
      Duong Long Duy, Yasuyuki Suda, Kenji Irie
    • Organizer
      日本細胞生物学会、日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of the role of PI4P and organelle contact site in prospore membrane extension during sporulation of budding yeast2018

    • Author(s)
      Tsuyoshi S. Nakamura, Kenji Muneshige, Yasuyuki Suda, Hiroyuki Tachikawa
    • Organizer
      日本細胞生物学会、日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 出芽酵母をモデルとした4Dイメージングで迫る真核生物の膜交通2018

    • Author(s)
      黒川量雄
    • Organizer
      酵母細胞研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 神経軸索ガイダンスを駆動する膜交通ダイナミクス2018

    • Author(s)
      戸島拓郎
    • Organizer
      電気通信大学化学生命工学セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 高速超解像顕微鏡法によるトランスゴルジ網の動態解析2018

    • Author(s)
      戸島拓郎
    • Organizer
      オルガネラゾーン研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 神経成長円錐における膜交通ダイナミクスの可視化解析2018

    • Author(s)
      戸島拓郎、宮代大輔、中野明彦
    • Organizer
      RAPシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Atg8による熱ストレス時の液胞膜陥入形成の抑制2018

    • Author(s)
      石井彩音、黒川量雄、堀田美友、中野明2、木村洋子
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Remarks] 中野明彦ラボ

    • URL

      http://sclim.riken.jp

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report

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Published: 2018-06-12   Modified: 2022-07-01  

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