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Expression control of virulence factor in Vibrio parahaemolyticus by linking stress response to iron and carbon source restrictions

Research Project

Project/Area Number 18K07130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionMatsuyama University

Principal Investigator

Tanabe Tomotaka  松山大学, 薬学部, 准教授 (60532786)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords腸炎ビブリオ / 鉄 / シデロフォア / 鉄制限ストレス / 炭素源制限ストレス / 転写因子 / 鉄獲得系 / 3型分泌装置 / 炭素源制御 / 炭素源 / CRP / 小分子RNA / 栄養制限ストレス
Outline of Final Research Achievements

Pathogenic bacteria are subjected to several environmental stresses in the host. Therefore, to understand the pathogenicity of bacteria, it is necessary to elucidate the control of virulence factor expression as well as the understanding of the response mechanism to multiple stresses. In this study, I analyzed the response factors of Vibrio parahaemolyticus to iron- and carbon-restricted stress and the expression control of virulence factors by them. As a result of this study, it was found in V. parahaemolyticus that CRP and DksA, which are carbon source metabolism regulators, and Fur, which is an iron metabolism regulator, regulate the expression of the iron acquisition system and also regulate the expression of two type 3 secretion systems in a coordinated manner. The results obtained in this study may contribute to a further understanding of the regulatory mechanism in the expression of pathogenicity of this bacterium.

Academic Significance and Societal Importance of the Research Achievements

病原細菌の外界環境変化の感知と応答に関する研究は、病原性発現や環境適応戦略の理解に繋がることから広く研究が行われている。しかし、病原因子の発現機構に関する多くの研究は、単独のストレス応答について解析したものが多く、複数のストレスを同時に受けたときの連携的応答機構についての研究はそれほど進んでいない。本研究から得た鉄制限及び炭素源制限ストレスに対する2つの3型分泌装置の発現機構は、腸炎ビブリオの3型分泌装置を介する病原性発現の詳細な理解と、腸管内における病原細菌の病原性発現制御機構についての新たな考えの提示に繋がる可能性があると考えている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] VuuB and IutB reduce ferric-vulnibactin in Vibrio vulnificus M27992020

    • Author(s)
      Okai Naoko、Miyamoto Katsushiro、Tomoo Koji、Tsuchiya Takahiro、Komano Jun、Tanabe Tomotaka、Funahashi Tatsuya、Tsujibo Hiroshi
    • Journal Title

      BioMetals

      Volume: 33 Issue: 4-5 Pages: 187-200

    • DOI

      10.1007/s10534-020-00241-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the heme acquisition system in Vibrio vulnificus M2799.2018

    • Author(s)
      Kawano H, Miyamoto K, Yasunobe M, Murata M, Yamahata E, Yamaguchi R, Miyaki Y, Tsuchiya T, Tanabe T, Funahashi T, Tsujibo H.
    • Journal Title

      Microb. Pathog.

      Volume: 117 Pages: 100-108

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of the ferric aerobactin receptor gene by a GntR-like repressor IutR in Vibrio furnissii.2018

    • Author(s)
      Tanabe T, Isshiki A, Miyamoto K, Tsujibo H, Yamamoto S, Funahashi T.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 365 Issue: 20

    • DOI

      10.1093/femsle/fny220

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 腸炎ビブリオのvibrioferrin生合成経路の解析2021

    • Author(s)
      田邊 知孝、見留 英路、宮本 勝城、長岡 憲次郎、舟橋 達也
    • Organizer
      日本薬学会 第141年会(広島)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 腸炎ビブリオのPvsA/B/D/E タンパク質によるvibrioferrinの生合成2019

    • Author(s)
      田邊知孝、山本重雄、舟橋達也
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] Vibrio vulnificusにおける転写調節因子DesRによるferrioxamine B受容体遺伝子desAの転写活性化機構2019

    • Author(s)
      田邊知孝、舟橋達也
    • Organizer
      第72回日本細菌学会中国・四国支部総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 病原ビブリオにおける シデロフォア利用系遺伝子の発現調節機構2019

    • Author(s)
      田邊知孝
    • Organizer
      第10回NAMEフォーラム
    • Related Report
      2019 Research-status Report
  • [Presentation] 腸炎ビブリオが産生するシデロフォア,vibrioferrinの生合成経路について2018

    • Author(s)
      田邊 知孝、山本 重雄、舟橋 達也
    • Organizer
      第71回日本細菌学会中国・四国支部総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Vibrioferrin生合成経路の解析2018

    • Author(s)
      田邊 知孝、山本 重雄、舟橋 達也
    • Organizer
      第52回ビブリオシンポジウム
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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