Importance of symbiotic microbes on the diversification of wood-dwelling insects

• Project/Area Number: 18K14473
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39050:Insect science-related
• Research Institution: Nagoya University
• Principal Investigator: Toki Wataru 名古屋大学, 生命農学研究科, 助教 (50611406)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 消化共生 / 栽培共生 / 木質 / 木材 / 酵母 / カミキリムシ / コメツキモドキ / ツツシンクイムシ / 共生 / 微生物

Outline of Final Research Achievements

Development of microbe-transferring organs and presence of symbiotic fungi in Lepturinae were examined. Xylose-assimilating yeasts were isolated from developed microbe-transferring organs of most lepturine species, suggesting the digestive mutualism between lepturine insects and yeasts. Species compositions of isolated yeasts were different among larval food habits, suggesting the different origin of mutualism.
Associated fungi were revealed for a ship timber beetle and a stag beetle.

Academic Significance and Societal Importance of the Research Achievements

森林において、多くの木材依存性昆虫が酵母と共生関係にあることを明らかにした。昆虫の利用する木材の部位や状態によって共生酵母が異なり、多様な昆虫－微生物共生関係があることを示した。

Research Products

• [Journal Article] A single case study of mycetangia-associated fungi and their abilities to assimilate wood-associated carbon sources in the ship timber beetle Elateroides flabellicornis (Coleoptera: Lymexylidae) in Japan (2021)
  • Author(s): Toki Wataru
  • Journal Title: Symbiosis Volume: 83 Issue: 2 Pages: 173-181
  • DOI: 10.1007/s13199-021-00745-9
  • Peer Reviewed
• [Journal Article] Time-dependent oviposition deterrence by an oviposition mark in a fungus-farming lizard beetle (2020)
  • Author(s): Miyazaki Yuta、Toki Wataru
  • Journal Title: The Science of Nature Volume: 107 Issue: 6 Pages: 53-53
  • DOI: 10.1007/s00114-020-01710-0
  • Peer Reviewed
• [Journal Article] Heads or tails: exaggerated morphologies in relation to the use of large bamboo internodes in two lizard beetles, Doubledaya ruficollis and Oxylanguria acutipennis (Coleoptera: Erotylidae: Languriinae) (2019)
  • Author(s): Toki Wataru、Matsuo Susumu、Pham Hong Thai、Meleng Paulus、Lee Chow-Yang
  • Journal Title: The Science of Nature Volume: 106 Issue: 9-10 Pages: 50-50
  • DOI: 10.1007/s00114-019-1645-6
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ハナカミキリ－酵母共生系の多様性：幼虫食性と酵母の種特異性 (2021)
  • Author(s): 岸上真子, 笹倉靖徳, 山迫淳介, 湯澤宣久, 土岐和多瑠
  • Organizer: 日本生態学会第68回大会
• [Presentation] 後から来てももう遅い？ニホンホホビロコメツキモドキの産卵場所を巡るメス間闘争 (2021)
  • Author(s): 宮崎雄太, 土岐和多瑠
  • Organizer: 日本生態学会第68回大会
• [Presentation] 竹林の昆虫－微生物共生系 (2021)
  • Author(s): 土岐和多瑠
  • Organizer: 第132回日本森林学会大会関連研究集会「第27回森林昆虫談話会」
  • Invited
• [Presentation] ハナカミキリ共生酵母の多様性と機能 (2020)
  • Author(s): 岸上真子, 土岐和多瑠
  • Organizer: 第67回日本生態学会大会
• [Presentation] クワガタムシと酵母の共生：共生酵母の維持における酵母運搬器官マイカンギアの役割 (2020)
  • Author(s): 山本大智、棚橋薫彦、深津武馬、土岐和多瑠
  • Organizer: 第67回日本生態学会大会
• [Presentation] コメツキモドキ（鞘翅目オオキノコムシ科）における大型の竹の利用と形態的特殊化 (2019)
  • Author(s): 土岐和多瑠, 松尾進, Pham Hong Thai, Paulus Meleng, Chow-Yang Lee
  • Organizer: 第10回日本甲虫学会 他 合同大会
• [Presentation] 訪花性ハナカミキリー酵母共生系における共生器官の発達と種特異性 (2019)
  • Author(s): 岸上真子, 土岐和多瑠
  • Organizer: 第10回日本甲虫学会 他 合同大会