• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

New approach for the detection of cellular stresses based on chaperone-carbohydrate interactions

Research Project

Project/Area Number 19350078
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionHokkaido University

Principal Investigator

NIIKURA Kenichi  Hokkaido University, 電子科学研究所, 准教授 (40360896)

Co-Investigator(Kenkyū-buntansha) IJIRO Kuniharu  北海道大学, 電子科学研究所, 教授 (90221762)
KOGURE Kentaro  京都薬科大学, 薬学部, 教授 (70262540)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords糖鎖 / 量子ドット / 核移行 / 細胞ストレス / シャペロン / 非特異吸着 / 分散性 / イメージング / N-アセチルグルコサミン
Research Abstract

For quantitative detection of stress responses to cells, we have developed sugar-displaying CdTe as a stress sensing probe in cells. During three years (H19-H21), we obtained following two results;
1. GlcNAc-QDs were accumulated in cells when stimulating the cells by adding heavy metals as chemical stresses.
2. The coating of QDs with α-Glucose can efficiently promote the nuclear import of QDs. This fact leads to various medical applications, such as drug delivery systems (DDSs) and bioimaging.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (60 results)

All 2010 2009 2008 2007

All Journal Article (27 results) (of which Peer Reviewed: 25 results) Presentation (28 results) Book (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Influence of Hydrophobic Structures on the Plasma Membrane Permeability of Lipid-like Molecules2010

    • Author(s)
      K. Niikura, K. Nambara, T. Okajima, Y. Matsuo, K. Ijiro
    • Journal Title

      Langmuir (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low pH-Triggered Model Drug Molecule Release from Virus-Like Particles2010

    • Author(s)
      N. Ohtake, K. Niikura, T. Suzuki, K. Nagakawa, S. Mikuni, Y. Matsuo, M. Kinjo, H. Sawa, K. Ijiro
    • Journal Title

      ChemBioChem 11

      Pages: 959-962

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low pH-Triggered Model Drug Molecule Release from Virus-Like Particles2010

    • Author(s)
      Noriko Ohtake
    • Journal Title

      ChemBioChem (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multivalent display of oligosaccharides on nanoparticles accelerates thier nuclear import2009

    • Author(s)
      K. Niikura, K. Ijiro, S. Sekiguchi
    • Journal Title

      Trends in Glycoscience and Glycotechnology 21

      Pages: 335-344

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gold nanoparticle arrangement on viral particles through carbohydrate recognition : A non-crosslinking approach to optical virus detection2009

    • Author(s)
      K. Niikura, K. Nagakawa, N Ohtake, T. Suzuki, Y. Matsuo, H. Sawa, K. Ijiro
    • Journal Title

      Bioconjugate Chem. 20

      Pages: 1848-1852

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Envelope-type lipid nanoparticles incorporating a short PEG-lipid conjugate for improved control of intracellular trafficking and transgene transcription2009

    • Author(s)
      T. Masuda, H. Akita, K. Niikura, T. Nishio, M. Ukawa, K. Enoto, R. Danev, K. Nagayama, K. Ijiro, H. Harashima
    • Journal Title

      Biomaterials 30

      Pages: 4806-4814

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Accumulation of Carbohydrate-Displaying CdTe Quantum Dots in Cells Responding to Cellular Chemical Stresses2009

    • Author(s)
      T. Nishio, K. Niikura, Y. Mastuo, K. Ijiro
    • Journal Title

      Int. J. Nanosci. 8

      Pages: 219-222

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design of cell-surface-retained polymers for artificial ligand display2009

    • Author(s)
      R. Kamitani, K. Niikura, T. Okajima, Y. Matsuo, K. Ijiro
    • Journal Title

      ChemBioChem 10

      Pages: 230-233

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced accumulation of carbohydratc-displaying CdTe quantum dots in cells responding to cellular chemical stresses2009

    • Author(s)
      Takashi Nishio
    • Journal Title

      International Journal of Nanoscience 8

      Pages: 219-222

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Envelope-type lipid nanoparticles incorporating a short PEG-lipid conjugate for improved control of intracellular trafficking and transgene transcription2009

    • Author(s)
      Tomoya Matsuda
    • Journal Title

      Biomaterials 30

      Pages: 4806-4814

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gold Nanoparticle Arrangement on Viral Particles through Carbohydrate Recognition : A Non-Cross-Linking Approach to Optical Virus Detection2009

    • Author(s)
      Kenichi Niikura
    • Journal Title

      Bioconjugate Chemistry 20

      Pages: 1848-1852

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multivalent display of oligosaccharides on nanoparticles accelerates thier nuclear import2009

    • Author(s)
      Kenichi Niikura
    • Journal Title

      Trends in Glycoscience and Glycotechnology 21

      Pages: 335-344

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      新倉謙一
    • Journal Title

      複合糖質の化学と最新応用技術(シーエムシー出版)

      Pages: 228-233

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      松尾保孝
    • Journal Title

      金属ナノ・マイクロ粒子の形状・構造制御技術(シーエムシー出版)

      Pages: 188-198

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Design of Cell-Surface-Retained Polymers for Artificial Ligand Display2009

    • Author(s)
      Ryosuke Kamitani
    • Journal Title

      ChemBioChem 10

      Pages: 230-233

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oligosaccharide-mediated Nuclear Transport of Nanoparticles2008

    • Author(s)
      K. Niikura, S. Sekiguchi, T. Nishio, T. Masuda, H. Akita, Y. Matsuo, K. Kogure, H. Harashima, K. Ijiro
    • Journal Title

      ChemBioChem 9

      Pages: 2623-2627

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stabilization of Multiassembly by Addition of a Phosphate Group at the 5'-Sticky End2008

    • Author(s)
      A. Tanaka, Y. Matsuo, K. Niikura, K. Ijiro
    • Journal Title

      Chem. Lett. 37(7)

      Pages: 758-759

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence-Specific Control of Azobenzene Assemblies by Molecular Recognition of DNA2008

    • Author(s)
      O. Haruta, Y. Matsuo, Y. Hashimoto, K. Niikura,K. Ijiro
    • Journal Title

      Langmuir 24(6)

      Pages: 2618-2624

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Cellular Uptake of Virus-Like Particles through Immobilization on a Sialic Acid-Displaying Solid Surface2008

    • Author(s)
      N. Ohtake, K. Niikura, T. Suzuki, K. Nagakawa, H. Sawa, K. Biro
    • Journal Title

      Bioconjugate Chem 19

      Pages: 507-515

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of lipid particles targeted viasugar-lipid conjugates as novel nuclear gene delivery system2008

    • Author(s)
      T. Masuda, H. Akita, T. Nishio, K. Niikura, K. Kogure, K. Ijiro, H. Harashima
    • Journal Title

      Biomaterials 29

      Pages: 709-723

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oligosaccharide-mediated Nuclear Transport of Nanoparticles2008

    • Author(s)
      Kenichi Niikura
    • Journal Title

      ChemBioChem 9

      Pages: 2623-2627

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stabilization of DNA Multiassembly by Addition of a Phosph ate Group at the 5'-Sticky End2008

    • Author(s)
      Aya Tanaka
    • Journal Title

      Chem. Lett. 37

      Pages: 758-759

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Cellular Uptake of Virus-Like Particles through Immobilization on a Sialic Acid-Displaying Solid Surface2008

    • Author(s)
      Noriko Ohtake
    • Journal Title

      Bioconjugate Chemistry 19

      Pages: 507-515

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequence-specific control of azobenzene assemblies by molecular recognition of DNA2008

    • Author(s)
      Osamu Haruta
    • Journal Title

      Langmuir 24

      Pages: 2618-2624

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of lipid particles targeted via sugar-lipid conjugates as novel nuclear gene delivery system2008

    • Author(s)
      Tomoya Masuda
    • Journal Title

      Biomaterials 29

      Pages: 709-723

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pattemed Immobilization of Unprotected Carbohydrates on an Aminooxy Polymer-Grafted Solid Surface2007

    • Author(s)
      R. Kamitani, K. Niikura, T. Onodera, N. Iwasaki, H. Shimaoka, K. Ijiro
    • Journal Title

      Bull. Chem. Soc. Jp 80(9)

      Pages: 1808-1813

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Patterned Immobilization of Unprotected Carbohydrates on an Aminooxy Polymer-Grafted Solid Surface2007

    • Author(s)
      Ryosuke Kamitani
    • Journal Title

      Bulletin of the Chemical Society of Japan 80

      Pages: 1808-1813

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウイルスの金属被覆とウイルスセンシングへの応用2010

    • Author(s)
      新倉謙一
    • Organizer
      田中貴金属工業記念講演
    • Place of Presentation
      田中貴金属工業 (東京)
    • Year and Date
      2010-04-27
    • Related Report
      2009 Final Research Report
  • [Presentation] pHに応答した分子放出のためのウイルスナノカプセルの機能化2010

    • Author(s)
      新倉謙一
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] pH に応答した分子放出のためのウイルスナノカプセルの機能化2010

    • Author(s)
      大竹範子, 新倉謙一, 鈴木忠樹, 三國新太郎, 松尾保孝, 永川桂太, 金城政孝, 澤洋文, 居城邦治
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Related Report
      2009 Final Research Report
  • [Presentation] Preparation of fuctionalized virus-like particles enabling pH-mediated release of target molecules2010

    • Author(s)
      Noriko Ohtake, Kenichi Niikura, Tadaki Suzuki, Shintaro Mikuni, Yasutaka Matsuo, Keita Nagakawa, Masataka Kinjo, Hirofumi Sawa, Kuniharu Ijiro
    • Organizer
      International Symposium of Joint Research Network on Advanced Materials and Devices "彫" [Chou]
    • Place of Presentation
      Hotel-Nidom, Hokkaido, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] ウイルスの金属被覆とウイルスセンシングへの応用2010

    • Author(s)
      新倉謙一
    • Organizer
      田中貴金属工業記念講演
    • Place of Presentation
      田中貴金属工業(東京)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Gold nanoparticle array based on the surface structure of virus2010

    • Author(s)
      永川桂大
    • Organizer
      International Symposium on Engineering Neo-Biomimetics
    • Place of Presentation
      独立行政法人産業技術総合研究所
    • Related Report
      2009 Annual Research Report
  • [Presentation] 分子内包ウイルスカプセルの作製とナノバイオロジーへの応用2009

    • Author(s)
      新倉謙一
    • Organizer
      岐阜大学生命工学科公開セミナー
    • Place of Presentation
      岐阜大学
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] オリゴ糖の高密度提示によって促進されるナノ微粒子の核内輸送2009

    • Author(s)
      関口翔太
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウイルスの糖鎖認識を利用したナノバイオテクノロジー2009

    • Author(s)
      新倉謙一
    • Organizer
      生体分子機能工学専攻特別講義講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2009-07-16
    • Related Report
      2009 Final Research Report
  • [Presentation] ウイルスの糖鎖認識を利用したナノバイオテクノロジー2009

    • Author(s)
      新倉謙一
    • Organizer
      生体分子機能工学專攻特別講義講演会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2009-07-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Sugar-Displaying Quantum Dots as a Bioimaging Tool2009

    • Author(s)
      K. Niikura
    • Organizer
      Recent Advances in Fluorescence Spectroscopic Methods for Biological and Chemical Systems
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-02-27
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Gold nanoparticle array based on the surface structure of virus2009

    • Author(s)
      K. Nagakawa, K. Niikura, N. Ohtake, T. Suzuki, Y. Matsuo, H. Sawa, K. Ijiro
    • Organizer
      International Symposium on Engineering Neo-Biomimetics-Toward Paradigm Shift for Innovation-
    • Place of Presentation
      AIST Tokyo Waterfront
    • Related Report
      2009 Final Research Report
  • [Presentation] オリゴ糖の高密度提示によって促進されるナノ微粒子の核内輸送2009

    • Author(s)
      関口翔太, 新倉謙一, 松尾保孝, 吉村成弘, 居城邦治
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本大学工学部
    • Related Report
      2009 Final Research Report
  • [Presentation] 細胞膜特異的な高分子を用いた細胞表層修飾法の開発2009

    • Author(s)
      新倉謙一, 南原克行, 神谷亮介, 岡嶋孝治, 居城邦治
    • Organizer
      第19回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学先端科学技術研究センター
    • Related Report
      2009 Final Research Report
  • [Presentation] Water soluble carbohydrate-displaying quantum dots for glycoprotein mimics in cells2008

    • Author(s)
      T. Nishio, K. Niikura, Y. Matsuo, H. Akita, H. Harashima, K. Ijiro
    • Organizer
      AsiaNANO2008 (Asian Conference on Nanoscience and Nanotechnology)
    • Place of Presentation
      Biopolis, Singapore
    • Related Report
      2009 Final Research Report
  • [Presentation] 糖鎖提示によるナノ微粒子・タンパクの核内移行促進2008

    • Author(s)
      新倉謙一
    • Organizer
      大阪大学蛋白質研究所セミナー「細胞機能制御システムとしての核膜研究~基礎から臨床応用を目指して~」
    • Place of Presentation
      大阪大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 糖鎖量子ドットプローブを用いた細胞ストレス可視化法の開発2008

    • Author(s)
      西尾崇, 新倉謙一, 秋田英万, 松尾保孝, 原島秀吉, 居城邦治
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] 糖鎖修飾量子ドットを用いた細胞内核内移行の解析2008

    • Author(s)
      関口翔太, 西尾崇, 新倉謙一, 松尾保孝, 居城邦治
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 糖鎖量子ドットプローブを用いた細胞ストレス可視化法の開発2008

    • Author(s)
      西尾崇
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      パシフイコ横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] 糖鎖提示によるナノ微粒子・タンパクの核内移行促進2008

    • Author(s)
      新倉謙一
    • Organizer
      平成21年蛋白研セミナー
    • Place of Presentation
      大阪大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] Water soluble carbohydrate-displaying quantum dots for glyco protein mimics in cells2008

    • Author(s)
      Takashi NISHIO
    • Organizer
      AsiaNANO2008
    • Place of Presentation
      Biopolis (Singapore)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 糖鎖提示量子ドットによる細胞ストレスのイメージング技術の開発2007

    • Author(s)
      西尾崇, 新倉謙一, 秋田英万, 松尾保孝, 原島秀吉, 居城邦治
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      東北大学多元物質科学研究所
    • Related Report
      2009 Final Research Report
  • [Presentation] 単一細胞のストレスをイメージングできる糖鎖プローブ分子の開発2007

    • Author(s)
      西尾崇, 新倉謙一, 松尾保孝, 秋田英万, 小暮健太朗, 原島秀吉, 居城邦治
    • Organizer
      第56回高分子討論会
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 細胞のストレスイメージングを可能とする鎖プローブの開発2007

    • Author(s)
      新倉謙一, 西尾崇, 松尾保孝, 秋田英万, 小暮健太朗, 原島秀吉, 居城邦治
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      九州大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 糖鎖半導体ナノ微粒子による細胞ストレス可視化技術の開発2007

    • Author(s)
      新倉謙一, 西尾崇, 秋田英万, 原島秀吉, 松尾保孝, 居城邦治
    • Organizer
      第17回バイオ・高分子シンポジウム
    • Place of Presentation
      上智大学
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 細胞のストレスイメージングを可能とする糖鎖プローブの開発2007

    • Author(s)
      新倉 謙一
    • Organizer
      第27回日本糖質学会
    • Place of Presentation
      九州大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 単一細胞のストレスをイメージングできる糖鎖ブローブ分子の開発2007

    • Author(s)
      西尾 崇
    • Organizer
      第56回高分子討論会
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 糖鎖提示量子ドットによる細胞ストレスのイメージング技術の開発2007

    • Author(s)
      新倉 謙一
    • Organizer
      第22回生体機能関連学部会シンポジウム
    • Place of Presentation
      東北大学
    • Related Report
      2007 Annual Research Report
  • [Book] 複合糖質の化学と最新応用技術2009

    • Author(s)
      新倉謙一
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 金属ナノ・マイクロ粒子の形状・構造制御技術2009

    • Author(s)
      松尾保孝, 新倉謙一, 居城邦治
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 量子ドットの生命科学領域への応用2007

    • Author(s)
      新倉謙一, 居城邦治
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 細胞ストレスのイメージングを目指した糖鎖修飾量子ドットの作製2007

    • Author(s)
      新倉 謙一
    • Total Pages
      9
    • Publisher
      シーエムシー出版
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 表面分子の識別方法、および識別分子2007

    • Inventor(s)
      岡嶋孝治
    • Industrial Property Rights Holder
      国立大学法人北海道大学
    • Industrial Property Number
      2007-226805
    • Filing Date
      2007-08-31
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi