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Development of injectable scaffolds including DNA/polycation complexes

Research Project

Project/Area Number 19390505
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionFukuoka Dental College

Principal Investigator

FUKUSHIMA Tadao  Fukuoka Dental College, 歯学部, 准教授 (80084250)

Co-Investigator(Kenkyū-buntansha) INOUE Yusuke  福岡医療短期大学, 歯科衛生学科, 准教授 (20105688)
HAYAKAWA Tohru  日本大学, 松戸歯学部, 准教授 (40172994)
OKAHATA Yoshio  東京工業大学, 生命理工学, 教授 (80038017)
DOI Yutaka  朝日大学, 歯学部, 教授 (40116067)
TAKEDA Shoji  大阪歯科大学, 歯学部, 准教授 (20067185)
KAWAGUCHI Minoru  福岡歯科大学, 歯学部, 助教 (10122780)
OHNO Jun  福岡歯科大学, 歯学部, 講師 (10152208)
TOYODA Mika  福岡歯科大学, 歯学部, 助教 (90412626)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
KeywordsDNA / ポリカチオン / 複合体 / スキャフォールド材 / インジェクショオン / 再生医療 / 粘性 / 多孔質 / インジェクショ / インジェクション
Research Abstract

The DNA/polycation complexes were prepared by reaction between salmon tests DNA and polycations (Chitosan, poly-L-arginine, poly-L-histidine, poly-L-lysine, and protamine) in order to investigated the potential of the complexes for injectable scaffolds. DNA/chitosan and DNA/protamine complexes were mobile paste. These complexes including carbonate apatite were also mobile paste, and created new bone in vivo test. It is expected that DNA/chitosan and DNA/protamine complexes will be useful as biomaterials for injectable scaffolds.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (29 results)

All 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (14 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 1 results)

  • [Journal Article] Bone response of DNA-chitosan-apatite complexes2009

    • Author(s)
      Minoru Kawaguchi, Jun Ohno, Teruaki Iwahashi, Tadao Fukushima, Tohru Hayakawa, Yutaka Doi
    • Journal Title

      J Oral Tissue Engin 7

      Pages: 89-98

    • NAID

      130004456610

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mold Fabrication and biological assessment of porous DNA-chitosan complexes2009

    • Author(s)
      Tadao Fukushima, Jun Ohno, Tohru Hayakawa, Minoru Kawaguchi, Yusuke Inoue, Shoji Takeda, Mika Toyoda, Yoshio Okahata
    • Journal Title

      J Biomed Mater Res Part B: Appl Biomater 91B

      Pages: 746-754

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mold Fabrication and biological assessment of porous DNA-chitosan complexes2009

    • Author(s)
      T.Fukushima, J.Ohno, T.Hayakawa, M.Kawaguchi, Y.Inoue, S.Takeda, M.Toyoda, Y.Okahata
    • Journal Title

      J Biomed Mater Res Part B : Appl Biomater 91B

      Pages: 746-754

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone response of DNA-chitosan-apatite complxes2009

    • Author(s)
      M.Kawaguchi, J.Ohno, T.Iwahashi, T.Fukushima, T.Hayakawa, Y.Doi
    • Journal Title

      J Oral Tissue Engin 7

      Pages: 89-98

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complexation of DNA with cationic polyamino acid for Biomaterial purposes2009

    • Author(s)
      T. Fukushima, M. Kawaguchi, T. Hayakawa, J. Ohno, T. Iwahashi, K. Taniguchi, Y. Inoue, S. Takeda
    • Journal Title

      Journal of Oral Tissue Engineering 6

      Pages: 24-32

    • NAID

      110007004836

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complexation of DNA with cationic polyamino acid for Biomaterial purposes2008

    • Author(s)
      Tadao Fukushima, Minoru Kawaguchi, Tohru Hayakawa, Jun Ohno, Teruaki Iwahashi, Kunihisa Taniguchi, Yusuke Inoue, Shoji Takeda
    • Journal Title

      J Oral Tissue Engin 6

      Pages: 24-32

    • NAID

      110007004836

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drug binding and releasing characteristics of DNA/lipid/PLGA film2007

    • Author(s)
      Tadao Fukushima, Minoru Kawaguchi, Tohru Hayakawa, Shoji Takeda, Yusuke Inoue, Jun Ohno, Kunihisa Taniguchi
    • Journal Title

      Dent Mater J 26

      Pages: 854-860

    • NAID

      110006557236

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drug binding and releasing characteristics of DNA/lipid/PLGA film2007

    • Author(s)
      T.Fukushima, M.Kawaguchi, T.Hayakawa, S, Takeda, Y.Inoue, J.Ohno, K.Taniguchi
    • Journal Title

      Dental Materials Journal 26

      Pages: 854-860

    • NAID

      110006557236

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and tissue response of lipid-coated b-FGF2007

    • Author(s)
      T.Hayakawa, T.Fukushima, M.Kawaguchi, Y.Inoue
    • Journal Title

      Journal of Oral Tissue Enginnering 5

      Pages: 96-103

    • NAID

      110006571215

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polycationic protamine for water-insoluble complex formation with DNA

    • Author(s)
      Tadao Fukushima, Jun Ohno, Tohru Hayakawa, Rieko Imayoshi, Minoru Kawaguchi, Yutaka Doi, Keiichi Kanaya, Makoto Mitarai
    • Journal Title

      Dent Mater J (in press)

    • NAID

      10026669680

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] DNA/プロタミン複合体の合成と組織親和性2009

    • Author(s)
      福島忠男、大野純、井上勇介, 川口稔、早川徹、御手洗誠, 岡畑恵雄
    • Organizer
      第31回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ京都テルサ(京都市)
    • Year and Date
      2009-11-17
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 賦形性に優れた生体材料;サケ白子由来DNAの加工性とその応用2009

    • Author(s)
      御手洗誠、福島忠男、川口稔、早川徹、岡畑恵雄
    • Organizer
      第31回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ京都テルサ(京都市)
    • Year and Date
      2009-11-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 賦形性に優れた生体材料;DNA/プロタミン複合体の加工性と生物学的性質2009

    • Author(s)
      御手洗誠、福島忠男
    • Organizer
      第131回日本歯科保存学会 2009年度秋季学術大会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] dna含有ペーストの歯科医療への応用事例2009

    • Author(s)
      福島忠男
    • Organizer
      第9回産学連携フェア
    • Place of Presentation
      (財)北九州産業学術推進機構産学連携c(北九州市)
    • Year and Date
      2009-10-28
    • Related Report
      2009 Final Research Report
  • [Presentation] DNA含有ペーストの歯科医療への応用事例2009

    • Author(s)
      福島忠男
    • Organizer
      第9回産学連携フェア
    • Place of Presentation
      (財)北九州産業学術推進機構 産学連携C(北九州市)
    • Year and Date
      2009-10-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 炭酸アパタイト含有DNA/プロタミン複合体の流動性と組織親和性2009

    • Author(s)
      福島忠男, 大野純, 井上勇介, 川口稔, 早川徹, 御手洗誠, 土井豊
    • Organizer
      第54回日本歯科理工学会学術講演会
    • Place of Presentation
      かごしま県民交流センター(鹿児島市)
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] DNA/プロタミン複合体の流動性と組織親和性2009

    • Author(s)
      福島忠男、大野純、川口稔、早川徹、御手洗誠
    • Organizer
      第7回日本再生歯科医学会学術大会
    • Place of Presentation
      九州歯科大学(北九州市)
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA/プロタミン複合体の合成とその生物学的性質2009

    • Author(s)
      福島忠男、大野純、井上勇介、川口稔、早川徹、御手洗誠
    • Organizer
      第53回日本歯科理工学会学術講演会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2009-04-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] DNA/キトサン複合体のインジェクタブルスキャホールド材への応用2008

    • Author(s)
      福島忠男, 大野純, 川口稔, 岩橋輝明, 井上勇介^3, 早川徹
    • Organizer
      第6回日本再生歯科医学会学術大会・総会
    • Place of Presentation
      日本歯科大学九段ホール・メモリアルホール(東京)
    • Year and Date
      2008-09-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNA/ポリアミノ酸複合体の調製とスキャホールド材への応用2008

    • Author(s)
      川口稔, 福島忠男, 大野純, 山崎純, 井上勇介, 早川徹
    • Organizer
      第6回日本再生歯科医学会学術大会・総会
    • Place of Presentation
      日本歯科大学九段ホール・メモリアルホール(東京)
    • Year and Date
      2008-09-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Preparation and characteristics of biocompatible DNA/chitosan transparent film2008

    • Author(s)
      M. Kawaguchi, T. Hayakawa, Y. Inoue, T. Fukushima, S. Takeda, J. Ohno, Y. Okahata
    • Organizer
      第8回世界バイオマテリアル学会
    • Place of Presentation
      アムステルダム(オランダ)
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 疑似体液中におけるDNA/キトサン複合体フィルム上へのアパタイト形成2008

    • Author(s)
      川口稔, 大野純, 早川徹, 井上勇介, 福島忠男
    • Organizer
      第51回日本歯科理工学会
    • Place of Presentation
      鶴見大学歯学部(横浜)
    • Year and Date
      2008-04-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNA/人工タンパク質複合体の合成と透明フィルム体の調製2007

    • Author(s)
      川口 稔・早川 徹・井上 勇介・福島 忠男・岡畑 恵雄
    • Organizer
      第29回日本バイオマテリアル学会大会
    • Place of Presentation
      大阪府豊中市(千里ライフサイエンスセンター)
    • Year and Date
      2007-11-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] DNA/キトサン複合体の特性および加工性2007

    • Author(s)
      早川 徹、福島 忠男、川口 稔、井上 勇介、岡畑 恵雄
    • Organizer
      高分子学会第36回医用高分子シンポジウム
    • Place of Presentation
      東京(上智大学)
    • Year and Date
      2007-07-31
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗菌歯周病剤及びそれを用いた医療用または歯科用材料2009

    • Inventor(s)
      福島忠男、早川徹、岡畑恵雄、御手洗誠、庵原啓司、江成宏之、関戸治知
    • Industrial Property Rights Holder
      福島忠男,(株)マルハニチロ食品
    • Filing Date
      2009-12-21
    • Acquisition Date
      2009-12-21
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 抗歯周病菌剤及びそれを用いた医療用または歯科用材料2009

    • Inventor(s)
      福島忠男、早川徹、岡畑恵雄、御手洗誠、庵原啓司、江成宏之、関戸治知
    • Industrial Property Rights Holder
      福島忠男、(株)マルハニチロ食品
    • Filing Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗歯周病菌剤及びそれを用いた医療用または歯科用材料2009

    • Inventor(s)
      福島忠男、早川徹、岡畑恵雄、御手洗誠、庵原啓司、江成宏之、関戸治知
    • Industrial Property Rights Holder
      福島忠男、(株)マルハニチロ食品
    • Filing Date
      2009-12-21
    • Related Report
      2009 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 抗菌歯周病剤及びそれを用いた医療用または歯科用材料2008

    • Inventor(s)
      福島忠男、早川徹、岡畑恵雄、御手洗誠、庵原啓司、江成宏之、関戸治知
    • Industrial Property Rights Holder
      福島忠男,(株)マルハニチロ食品
    • Filing Date
      2008-12-19
    • Acquisition Date
      2008-12-19
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 抗菌歯周病剤及びそれを用いた医療用または歯科用材料2008

    • Inventor(s)
      福島忠男, 早川徹, 岡畑恵雄, 御手洗誠, 庵原啓司, 江成宏之, 関戸治知
    • Industrial Property Rights Holder
      福島忠男, (株)マルハニチロ
    • Industrial Property Number
      2008-324524
    • Filing Date
      2008-12-19
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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