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A novel transgenic animal to visualize muscular activity upon physical exercise.

Research Project

Project/Area Number 19500560
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionKanazawa University

Principal Investigator

HITOMI Yoshiaki  Kanazawa University, 医学系, 准教授 (70231545)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hiroyuki  金沢大学, 医学系, 教授 (30231476)
KAMBAYASHI Yasuhiro  金沢大学, 医学系, 講師 (20345630)
HIBINO Yuri  金沢大学, 医学系, 助教 (40362008)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords遺伝子組換え動物 / 運動 / 姿勢保持 / 骨格筋 / 遺伝子発現 / 遺伝子改変動物 / 姿勢維持 / レポーター遺伝子
Research Abstract

運動における骨格筋の役割や相互の関係を調べるために、運動感受性遺伝子Rcan1遺伝子のプロモーターを用いた遺伝子改変動物の作出を行った。その結果、短半減期レポーター遺伝子が組み込まれた9系統の動物ファウンダーが得られ、7系統を維持することに成功した。これらの動物では、Rcan1プロモーター制御下に短半減期レポーターの発現が観察された。今後、運動、姿勢制御における骨格筋の視覚的、定量的解析に寄与することが期待される。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (26 results)

All 2008 2007

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (5 results)

  • [Journal Article] Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta2008

    • Author(s)
      Hitomi Y, Watanabe S, Kizaki T, Ohno H(他5人・1番目)
    • Journal Title

      Redox Rep 13

      Pages: 213-216

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate2008

    • Author(s)
      Kambayashi Y, Hibino Y, Hitomi Y, Nakamura H(他3人・1番目)
    • Journal Title

      J Clin Biochem Nutr 44

      Pages: 46-51

    • NAID

      130004466533

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of a polyclonal antibody against brominated protein2008

    • Author(s)
      Kambayashi Y, Hibino Y, Hitomi Y, Nakamura H(他5人・1番目)
    • Journal Title

      J Clin Biochem Nutr 44

      Pages: 95-103

    • NAID

      130004466540

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell2008

    • Author(s)
      Hirota R, Akimaru K, Nakamura H
    • Journal Title

      Toxicol In Vitro 22(4)

      Pages: 988-994

    • NAID

      120001940962

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta.2008

    • Author(s)
      Hitomi Y
    • Journal Title

      Redox Rep. 13

      Pages: 213-216

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association between physical restraint and drug-induced liver injury2008

    • Author(s)
      Hatta K
    • Journal Title

      Neuropsychobiol. 56

      Pages: 180-184

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] n vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell.2008

    • Author(s)
      Hirota R
    • Journal Title

      Toxicol In Vitro. 22

      Pages: 988-994

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate.2008

    • Author(s)
      Kambayashi Y
    • Journal Title

      J Clin Biochem Nutr 44

      Pages: 46-51

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation and characterization of a polyclonal antibody against brominated protein.2008

    • Author(s)
      Kambayashi Y
    • Journal Title

      J Clin Biochem Nutr 44

      Pages: 95-103

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD2007

    • Author(s)
      Hitomi Y, Kambayashi Y, Hibino Y(他7人・1番目)
    • Journal Title

      J Biochem 141

      Pages: 495-502

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma2007

    • Author(s)
      Takemoto K, Hitomi Y, Kambayashi Y.(他7人・5番目)
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol 293

      Pages: 1419-1426

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of reactive nitrogen species by eosinophil peroxidase in tyrosine nitration2007

    • Author(s)
      Takemoto K, Kambayashi Y, Hibino Y, Hitomi Y(他5人・6番目)
    • Journal Title

      Acta Medica Okayama 61

      Pages: 17-30

    • NAID

      120002312059

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis2007

    • Author(s)
      Nakamura H.(他13人・1番目)
    • Journal Title

      Int Arch Allergy Immunol 142

      Pages: 329-334

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Various molecular species of diacylglycerol hydroperoxide activate human neutrophils via PKC activation2007

    • Author(s)
      Kambayashi Y, Hitomi Y(他7人・1番目)
    • Journal Title

      J Clin Biochem Nutr 41

      Pages: 68-75

    • NAID

      130004879123

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD2007

    • Author(s)
      Hitomi Y
    • Journal Title

      J Biochem. 141

      Pages: 495-502

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of reactive nitrogen species by eosinophil peroxidase in tyrosine nitration2007

    • Author(s)
      Takemoto K
    • Journal Title

      Acta Medica Okayama 61

      Pages: 17-30

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma2007

    • Author(s)
      Takernoto K
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol. 293

      Pages: 1419-1426

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between mental health of Japanese residents and the quality of medical service2007

    • Author(s)
      Yoshino S
    • Journal Title

      J Phys Fit Nutri Immunol. 7

      Pages: 3-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis2007

    • Author(s)
      Nakamura H
    • Journal Title

      Int Arch Allergy Immunol. 142

      Pages: 329-334

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Psychological factors including sense of coherence and some lifestyles are related to General Health Questionnaire-12 (GHQ-12) in elderly workers in Japan2007

    • Author(s)
      Matsuzaki I
    • Journal Title

      Environ Health Prev Med. 12

      Pages: 71-77

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Various molecular species of diacylglycerol hydroperoxide activate human neutrophils via PKC activation2007

    • Author(s)
      Kambayashi Y
    • Journal Title

      J Clin Biochem Nutr. 41

      Pages: 68-75

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨格筋に対するin vivo遺伝子導入法によるCalcineurin制御タンパクRcan1発現制御の検討2008

    • Author(s)
      人見嘉哲ら
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] 骨格筋に対するin vivo 遺伝子導入法によるCalcineur in制御タンパクRcanl発現制御の検討2008

    • Author(s)
      人見嘉哲
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] カルシニューリン制御タンパクRcn1による骨格筋活動のモニター2007

    • Author(s)
      人見嘉哲ら
    • Organizer
      文部科学省学術フロンティア研究プロジェクト「ライフステージに応じた健康増進と多様性保持」第2回研究会
    • Place of Presentation
      所沢
    • Year and Date
      2007-12-22
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 好酸球活性化マーカーである(ジ)ブロモチロシンを認識する抗体の作成2007

    • Author(s)
      神林康弘ら
    • Organizer
      第5回日本予防医学会学術総会
    • Place of Presentation
      指宿
    • Year and Date
      2007-11-23
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 簡便な血漿総抗酸化能測定法の開発2007

    • Author(s)
      神林康弘ら
    • Organizer
      第29回日本フリーラジカル学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-06-10
    • Related Report
      2008 Final Research Report 2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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