A novel transgenic animal to visualize muscular activity upon physical exercise.

• Project/Area Number: 19500560
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Kanazawa University
• Principal Investigator: HITOMI Yoshiaki Kanazawa University, 医学系, 准教授 (70231545)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Hiroyuki 金沢大学, 医学系, 教授 (30231476) ; KAMBAYASHI Yasuhiro 金沢大学, 医学系, 講師 (20345630) ; HIBINO Yuri 金沢大学, 医学系, 助教 (40362008)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 遺伝子組換え動物 / 運動 / 姿勢保持 / 骨格筋 / 遺伝子発現 / 遺伝子改変動物 / 姿勢維持 / レポーター遺伝子

Research Abstract

運動における骨格筋の役割や相互の関係を調べるために、運動感受性遺伝子Rcan1遺伝子のプロモーターを用いた遺伝子改変動物の作出を行った。その結果、短半減期レポーター遺伝子が組み込まれた9系統の動物ファウンダーが得られ、7系統を維持することに成功した。これらの動物では、Rcan1プロモーター制御下に短半減期レポーターの発現が観察された。今後、運動、姿勢制御における骨格筋の視覚的、定量的解析に寄与することが期待される。

Research Products

• [Journal Article] Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta (2008)
  • Author(s): Hitomi Y, Watanabe S, Kizaki T, Ohno H(他5人・1番目)
  • Journal Title: Redox Rep 13 Pages: 213-216
  • Peer Reviewed
• [Journal Article] Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate (2008)
  • Author(s): Kambayashi Y, Hibino Y, Hitomi Y, Nakamura H(他3人・1番目)
  • Journal Title: J Clin Biochem Nutr 44 Pages: 46-51
  • NAID: 130004466533
  • Peer Reviewed
• [Journal Article] Preparation and characterization of a polyclonal antibody against brominated protein (2008)
  • Author(s): Kambayashi Y, Hibino Y, Hitomi Y, Nakamura H(他5人・1番目)
  • Journal Title: J Clin Biochem Nutr 44 Pages: 95-103
  • NAID: 130004466540
  • Peer Reviewed
• [Journal Article] In vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell (2008)
  • Author(s): Hirota R, Akimaru K, Nakamura H
  • Journal Title: Toxicol In Vitro 22(4) Pages: 988-994
  • NAID: 120001940962
  • Peer Reviewed
• [Journal Article] Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta. (2008)
  • Author(s): Hitomi Y
  • Journal Title: Redox Rep. 13 Pages: 213-216
  • Peer Reviewed
• [Journal Article] Association between physical restraint and drug-induced liver injury (2008)
  • Author(s): Hatta K
  • Journal Title: Neuropsychobiol. 56 Pages: 180-184
  • Peer Reviewed
• [Journal Article] n vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell. (2008)
  • Author(s): Hirota R
  • Journal Title: Toxicol In Vitro. 22 Pages: 988-994
  • Peer Reviewed
• [Journal Article] Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate. (2008)
  • Author(s): Kambayashi Y
  • Journal Title: J Clin Biochem Nutr 44 Pages: 46-51
  • Peer Reviewed
• [Journal Article] Preparation and characterization of a polyclonal antibody against brominated protein. (2008)
  • Author(s): Kambayashi Y
  • Journal Title: J Clin Biochem Nutr 44 Pages: 95-103
  • Peer Reviewed
• [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD (2007)
  • Author(s): Hitomi Y, Kambayashi Y, Hibino Y(他7人・1番目)
  • Journal Title: J Biochem 141 Pages: 495-502
  • Peer Reviewed
• [Journal Article] Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma (2007)
  • Author(s): Takemoto K, Hitomi Y, Kambayashi Y.(他7人・5番目)
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 293 Pages: 1419-1426
  • Peer Reviewed
• [Journal Article] Biochemical characterization of reactive nitrogen species by eosinophil peroxidase in tyrosine nitration (2007)
  • Author(s): Takemoto K, Kambayashi Y, Hibino Y, Hitomi Y(他5人・6番目)
  • Journal Title: Acta Medica Okayama 61 Pages: 17-30
  • NAID: 120002312059
  • Peer Reviewed
• [Journal Article] Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis (2007)
  • Author(s): Nakamura H.(他13人・1番目)
  • Journal Title: Int Arch Allergy Immunol 142 Pages: 329-334
  • Peer Reviewed
• [Journal Article] Various molecular species of diacylglycerol hydroperoxide activate human neutrophils via PKC activation (2007)
  • Author(s): Kambayashi Y, Hitomi Y(他7人・1番目)
  • Journal Title: J Clin Biochem Nutr 41 Pages: 68-75
  • NAID: 130004879123
  • Peer Reviewed
• [Journal Article] Disposition of protein-bound 3-nitrotyrosine in rat plasma analyzed by a novel protocol for HPLC-ECD (2007)
  • Author(s): Hitomi Y
  • Journal Title: J Biochem. 141 Pages: 495-502
  • Peer Reviewed
• [Journal Article] Biochemical characterization of reactive nitrogen species by eosinophil peroxidase in tyrosine nitration (2007)
  • Author(s): Takemoto K
  • Journal Title: Acta Medica Okayama 61 Pages: 17-30
  • Peer Reviewed
• [Journal Article] Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma (2007)
  • Author(s): Takernoto K
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. 293 Pages: 1419-1426
  • Peer Reviewed
• [Journal Article] Relationship between mental health of Japanese residents and the quality of medical service (2007)
  • Author(s): Yoshino S
  • Journal Title: J Phys Fit Nutri Immunol. 7 Pages: 3-11
  • Peer Reviewed
• [Journal Article] Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis (2007)
  • Author(s): Nakamura H
  • Journal Title: Int Arch Allergy Immunol. 142 Pages: 329-334
  • Peer Reviewed
• [Journal Article] Psychological factors including sense of coherence and some lifestyles are related to General Health Questionnaire-12 (GHQ-12) in elderly workers in Japan (2007)
  • Author(s): Matsuzaki I
  • Journal Title: Environ Health Prev Med. 12 Pages: 71-77
  • Peer Reviewed
• [Journal Article] Various molecular species of diacylglycerol hydroperoxide activate human neutrophils via PKC activation (2007)
  • Author(s): Kambayashi Y
  • Journal Title: J Clin Biochem Nutr. 41 Pages: 68-75
  • Peer Reviewed
• [Presentation] 骨格筋に対するin vivo遺伝子導入法によるCalcineurin制御タンパクRcan1発現制御の検討 (2008)
  • Author(s): 人見嘉哲ら
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 骨格筋に対するin vivo 遺伝子導入法によるCalcineur in制御タンパクRcanl発現制御の検討 (2008)
  • Author(s): 人見嘉哲
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] カルシニューリン制御タンパクRcn1による骨格筋活動のモニター (2007)
  • Author(s): 人見嘉哲ら
  • Organizer: 文部科学省学術フロンティア研究プロジェクト「ライフステージに応じた健康増進と多様性保持」第2回研究会
  • Place of Presentation: 所沢
  • Year and Date: 2007-12-22
• [Presentation] 好酸球活性化マーカーである(ジ)ブロモチロシンを認識する抗体の作成 (2007)
  • Author(s): 神林康弘ら
  • Organizer: 第5回日本予防医学会学術総会
  • Place of Presentation: 指宿
  • Year and Date: 2007-11-23
• [Presentation] 簡便な血漿総抗酸化能測定法の開発 (2007)
  • Author(s): 神林康弘ら
  • Organizer: 第29回日本フリーラジカル学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-06-10