Genome analyses of nucleus and mitochondria in the dinoflagellate Noctiluca scintillans

• Project/Area Number: 19580203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kanazawa University
• Principal Investigator: ENDOH Hiroshi Kanazawa University, 自然システム学系, 准教授 (20272932)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 進化 / 原生生物 / アルベオラータ / 渦鞭毛虫 / 核ゲノム / ミトコンドリアゲノム / ゲノム / 核 / ミトコンドリア / 赤潮 / 繊毛虫 / 核分化

Research Abstract

Genome analyses of nucleus and mitochondria in ancestral dinoflagellates were carried out. Phylogenetic analyses using 2 available protein-coding genes showed that Noctiluca synthillans is the next earliest branch after Oxyrrhis marina. On the other hand, mitochondrial genome analysis of O.marina revealed several peculiar natures of mtDNA. Oxyrrhis mtDNA consists of various kinds of minicircle with the sizes of～some kb and total number of genes encoded is only 4.

Research Products

• [Journal Article] Gigantic macroautophagy in programmed nuclear death of Tetrahymena thermophila. (2010)
  • Author(s): Akematsu T, Pearlman R.E, Endoh H
  • Journal Title: Autophagy 6 Pages: 901-911
  • Peer Reviewed
• [Journal Article] Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila. (2010)
  • Author(s): Akematsu T, Endoh H
  • Journal Title: BMC Cell Biol. 11 Pages: 13-27
  • Peer Reviewed
• [Journal Article] Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila. (2010)
  • Author(s): Takahiko Akematsu, Hiroshi Endoh
  • Journal Title: BMC Cell Biology Volume: 11 Pages: 13-27
  • Peer Reviewed
• [Journal Article] Gigantic macroautophagy in programmed nuclear death of Tetrahymena thermophila. (2010)
  • Author(s): Takahiko Akematsu, Ronald E.Pearlman, Hiroshi Endoh
  • Journal Title: Autophagy Volume: 6 Pages: 901-911
  • Peer Reviewed
• [Journal Article] Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjug ation in Tetrahymena thermophila (2010)
  • Author(s): Akematsu T, Endoh H
  • Journal Title: BMC Cell Biol. 11 Pages: 13-27
  • Peer Reviewed
• [Journal Article] Role of apoptosis-inducing factor(AIF)in programmed nuclear death during conjugation in Tetrahymena thermophila (2010)
  • Author(s): Takahiko Akematsu, Hiroshi Endoh
  • Journal Title: BMC Cell Biology 11 Pages: 13-27
  • Peer Reviewed
• [Journal Article] Phylogenetic analyses of the dinoflagellate No ctiluca scintillans based on beta-tubulin and Hsp90 genes. (2008)
  • Author(s): Fukuda Y, Endoh H
  • Journal Title: Eur.J.Protistol. 42 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Differentially expressed genes during fruiting body development in the aggregative ciliate Sorogena stoianovitchae (Ciliophora : Colpodea). (2008)
  • Author(s): Sugimoto H, Endoh H
  • Journal Title: J.Eukaryot.Microbiol. 42 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Phylogenetic analyses of the dinoflagellate Noctiluca scintillans (based on beta-tubulin and Hsp90 genes) (2008)
  • Author(s): Fukuda Y, Endoh H
  • Journal Title: Eur.J.Protistol. 42 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Differentially expressed genes during fruiting body development in the aggregative ciliate Sorogena stoianovitc hae (Ciliophora : Colpodea) (2008)
  • Author(s): Sugimoto H, Endoh H
  • Journal Title: Eur.J.Protistol. 42 Pages: 209-219
  • Peer Reviewed
• [Journal Article] Differentially expressed genes during fruiting body development in the aggregatitive ciliate Sorogena stoianovitchae (Ciliophora : Colpodea) (2008)
  • Author(s): Sugimoto H, Endoh H
  • Journal Title: J. Eukaryot. Microbiol. 55 Pages: 110-116
  • Peer Reviewed
• [Journal Article] Differentially expressed genes during fruiting body development in the aggregatitive ciliate Sorogena stoianovitchae (Ciliophora: Colpodea) (2008)
  • Author(s): Sugimoto H, Endoh H
  • Journal Title: J.Eukaryot.Microbiol. 55 Pages: 110-116
  • Peer Reviewed
• [Journal Article] Phylogenetic analyses of the dinoflagellate Noctiluca scintillans based on β-tubulin and Hsp90 genes (2008)
  • Author(s): Fukuda Y, Endoh H
  • Journal Title: Eur.J.Protistol. 44 Pages: 27-33
  • Peer Reviewed
• [Presentation] ミトコンドリアゲノムからみた渦鞭毛虫類とアピコンプレクサ類の初期進化 (2010)
  • Author(s): 北田菜穂美、遠藤浩
  • Organizer: 第43回日本原生動物学会
  • Place of Presentation: 茨城大学(茨城県)
  • Year and Date: 2010-11-06
• [Presentation] ミトコンドリアゲノムからみた渦鞭毛虫類とアピコンプレクサ類の初期進化 (2010)
  • Author(s): 北田菜穂美、遠藤浩
  • Organizer: 第43回日本原生動物学会
  • Place of Presentation: 茨城大学理学部、水戸
  • Year and Date: 2010-11-06
• [Presentation] 祖先的繊毛虫原子大核類Loxodesの培養法の検討 (2010)
  • Author(s): 吉野亜紀、遠藤浩
  • Organizer: 第43回日本原生動物学会
  • Place of Presentation: 茨城大学理学部、水戸
  • Year and Date: 2010-11-06
• [Presentation] テトラヒメナの核退化におけるミトコンドリア内在性ヌクレアーゼの役割 (2010)
  • Author(s): 長田恵梨子、明松隆彦、遠藤浩
  • Organizer: 第43回日本原生動物学会
  • Place of Presentation: 茨城大学理学部、水戸
  • Year and Date: 2010-11-06
• [Presentation] 原生動物テトラヒメナによるセルロース分解系 (2010)
  • Author(s): 中島千佳、守屋繁春、仁宮一章、遠藤浩
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: シーガイア、宮崎
  • Year and Date: 2010-10-29
• [Presentation] シロアリ共生鞭毛虫の遺伝子群を導入したテトラヒメナによるセルロース分解系の構築 (2010)
  • Author(s): 遠藤浩、中島千佳
  • Organizer: 日本動物学会第81回東京大会2010サデライトシンポジウム「原生生物は未来を拓くバイオマシン」
  • Place of Presentation: 東京大学教養学部 招待講演
  • Year and Date: 2010-09-24
• [Presentation] Mitochondrial genome of the ancestral dinoflagellate Oxyrrhis marina (2010)
  • Author(s): Kitada N, Fukuda Y, Endoh H.
  • Organizer: The 18th International Society for Evolutionary Protistology
  • Place of Presentation: 石川県立美術館(石川県)
  • Year and Date: 2010-07-06
• [Presentation] Mitochondrial genome of the ancestral dinoflagellate Oxyrrhis marina. (2010)
  • Author(s): Nahomi Kitada, Yasuhiro Fukuda, Hiroshi Endoh
  • Organizer: The 18^<th> Meeting of the International Society for Evolutionary Protistology
  • Place of Presentation: 石川県立美術館、金沢
  • Year and Date: 2010-07-06
• [Presentation] 大核の起源:トランスポゾン侵入説 (2009)
  • Author(s): 遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学、石巻(宮城県)
  • Year and Date: 2009-11-01
• [Presentation] 祖先的渦鞭毛虫 Oxyrrhis marina のミトコンドリアゲノム解析 (2009)
  • Author(s): 北田菜穂美、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] 祖先的渦鞭毛虫 Oxyrrhis marina のミトコンドリアゲノム解析 (2009)
  • Author(s): 北田菜穂美, 遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学 (宮城県)
  • Year and Date: 2009-10-31
• [Presentation] テトラヒメナを利用した異種タンパク質分泌の試み～セルロース分解系構築へ向けて～ (2009)
  • Author(s): 中島千佳、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学、石巻(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] テトラヒメナの核退化からみたアポトーシスの捉え方 (2009)
  • Author(s): 明松隆彦、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学、石巻(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] テトラヒメナの核アポトーシスに関わるミトコンドリア局在性ヌクレアーゼの同定 (2009)
  • Author(s): 長田恵梨子、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学、石巻(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] ゾウリムシの接合初期過程に発現するmRNA型non-coding RNA遺伝子 (2009)
  • Author(s): 長谷川寛、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学、石巻(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] 祖先的渦鞭毛虫Oxyrrhis marinaのミトコンドリアゲノム解析 (2009)
  • Author(s): 北田菜穂美、遠藤浩
  • Organizer: 第42回日本原生動物学会
  • Place of Presentation: 石巻専修大学・石巻(宮城県)
  • Year and Date: 2009-10-31
• [Presentation] New details from the complete life cycle and gamete morphologies of the red tide dinoflagellate Noctiluca scintillans (Ehrenberg) McCartney (2009)
  • Author(s): Fukuda Y, Endoh H.
  • Organizer: The 13th International Congress of Protistology
  • Place of Presentation: Hotel Atlantico Buzios Convention and Resort,(ブラジル)
  • Year and Date: 2009-08-26
• [Presentation] Role of apoptosis-inducing factor in programmed nuclear death during conjugation in Tetrahymena thermophila (2009)
  • Author(s): Takahiko Akematsu, Hiroshi Endoh
  • Organizer: 2009 FASEB Summer Research Conferences(Ciliate Molecular Biology)
  • Place of Presentation: Vermont Academy, Saxtons River, Vermont, USA
  • Year and Date: 2009-07-27
• [Presentation] 渦鞭毛虫Oxyrrhis marinaのミトコンドリアゲノム解析 (2009)
  • Author(s): 北田菜穂美、遠藤浩
  • Organizer: 第4回日本進化原生生物学研究会
  • Place of Presentation: 宮城教育大学、仙台(宮城県)
  • Year and Date: 2009-07-04
• [Presentation] 繊毛虫テトラヒメナの核アポトーシスにおけるAIFの役割 (2008)
  • Author(s): 明松隆彦, 遠藤浩
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 奈良
  • Year and Date: 2008-12-12
• [Presentation] ヤコウチュウの生活環と渦鞭毛虫の初期進化 (2008)
  • Author(s): 福田康弘、洲崎敏伸、遠藤浩
  • Organizer: ユーグレナ研究会第24回研究集会
  • Place of Presentation: 近畿大学(奈良県)
  • Year and Date: 2008-11-15
• [Presentation] ヤコウチュウの生活環と渦鞭毛虫の初期進化 (2008)
  • Author(s): 福田康弘, 洲崎敏伸, 遠藤浩
  • Organizer: ユーグレナ研究会第24回研究集会
  • Place of Presentation: 奈良
  • Year and Date: 2008-11-15
• [Presentation] Evolution of apoptosis : a case study of nuclear apoptosis in ciliates (2008)
  • Author(s): Hiroshi Endoh, Takahiko Akematsu, Takashi Kobayashi
  • Organizer: International Symposium on Protistology : Evolution and Diversity
  • Place of Presentation: つくば、茨城
  • Year and Date: 2008-11-09
• [Presentation] テトラヒメナの核アポトーシス初期過程の実行因子はミトコンドリアに存在する (2008)
  • Author(s): 明松隆彦, 遠藤浩
  • Organizer: 日本原生動物学会
  • Place of Presentation: つくば、茨城
  • Year and Date: 2008-11-02
• [Presentation] 原生生物テトラヒメナの核アポトーシスにおけるAIFの役割 (2008)
  • Author(s): 明松隆彦, 遠藤浩
  • Organizer: 日本アポトーシス研究会
  • Place of Presentation: 京都
  • Year and Date: 2008-08-02
• [Presentation] Conjugation-specific expression of GRP94 homologue (PcGRP92) in Paramecium caudatum is regulated by a short element located with in thecoding region (2008)
  • Author(s): Sanae Nakayama, Manabu Hori, Hiroki Sugimoto, Hiroshi Endoh
  • Organizer: Protist 2008 Conference
  • Place of Presentation: ハリファックス、カナダ
  • Year and Date: 2008-07-24
• [Presentation] Theoretical consideration of early history of dinoflagellates : Haploid core-dinoflagellates might have evolved from a zoospore of ancestral diploid dinoflagellates via neoteny (2008)
  • Author(s): Fukuda Y, Suzaki T, Endoh H.
  • Organizer: Protist 2008 Conference (The 59th International Society of Protistology and the 17th International Society for Evolutionary Protistology, Joint conference)
  • Place of Presentation: Dalhausie University (カナダ)
  • Year and Date: 2008-07-23
• [Presentation] Theoretical consideration of early history of dinoflagellates : Haploid coredinoflagellates might have evolved from a zoospore of ancestral diploid dinoflagellates via neoteny (2008)
  • Author(s): Fukuda Y, Suzaki T, Endoh H.
  • Organizer: Protist2008 (ISOP 59^<th> and ISEP17^<th> joint conference)
  • Place of Presentation: Dalhousie University, Halifax (Canada)
  • Year and Date: 2008-07-23
• [Presentation] Theoretical consideration of early history of dinoflagellates : Haploid core-dinoflagellates might have evolved from a zoospore of ancestral diploiddinoflagellates via neoteny (2008)
  • Author(s): Yasuhiro FUKUDA, Toshinobu SUZAKI, Hiroshi ENDOH
  • Organizer: Protist 2008 Conference
  • Place of Presentation: ハリファックス、カナダ
  • Year and Date: 2008-07-23
• [Presentation] 渦鞭毛虫の初期進化:遊走子のネオテニーとコア渦鞭毛虫の出現 (2007)
  • Author(s): 福田康弘、遠藤浩
  • Organizer: 第40回日本原生動物学会
  • Place of Presentation: 富山大学(富山県)
  • Year and Date: 2007-11-18
• [Presentation] 渦鞭毛虫の初期進化:遊走子のネオテニーとコア渦鞭毛虫の出現 (2007)
  • Author(s): 福田 康弘、遠藤 浩
  • Organizer: 第40回日本原生動物学会
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2007-11-18
• [Presentation] Sorogena stoianovitchaeの子実体形成に関わる糖鎖の探索 (2007)
  • Author(s): 杉本 大樹、遠藤 浩
  • Organizer: 第40回日本原生動物学会
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2007-11-18
• [Presentation] ゾウリムシの接合初期に特異的に発現する熱ショック関連遺伝子 (2007)
  • Author(s): 中山 早苗、堀 学、杉本 大樹、遠藤 浩
  • Organizer: 第40回日本原生動物学会
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2007-11-18
• [Presentation] Life cycle and phylogenetic position of dinoflagellate (Noctiluca scintillans). (2007)
  • Author(s): Fukuda Y, Endoh H.
  • Organizer: The 5th European Congress of Protistology and the 11th European Conference on Ciliate Biology (joint conference)
  • Place of Presentation: Saint Petersburg State University(ロシア)
  • Year and Date: 2007-07-24
• [Presentation] Life cycle and phylogenetic position of dinoflagellate (Noctiluca scintillans) (2007)
  • Author(s): Fukuda Y, Endoh H
  • Organizer: V European Congress of Protistology and XI European Conferenceon Ciliate Biology
  • Place of Presentation: サンクトペテルブルク、ロシア
  • Year and Date: 2007-07-24
• [Presentation] Genome Plasticity in the Dicyemid Mesozoans (2007)
  • Author(s): Awata T, Noto T, Endoh H
  • Organizer: V European Congress of Protistology and XI European Conference on Ciliate Biology
  • Place of Presentation: サンクトペテルブルク、ロシア
  • Year and Date: 2007-07-24
• [Presentation] ヤコウチュウ(Noctiluca scintillans)の全生活環, および形態と分子からみた系統的位置 (2007)
  • Author(s): 福田康弘、遠藤浩
  • Organizer: 第3回日本進化原生生物学研究会
  • Place of Presentation: 海洋研究開発機構横浜研究所(神奈川県)
  • Year and Date: 2007-07-07
• [Presentation] ヤコウチュウ(Noctiluca scinti!lans)の全生活環,および形態と分子からみた系統的位置 (2007)
  • Author(s): 福田 康弘、遠藤 浩
  • Organizer: 第3回日本進化原生生物学研究会
  • Place of Presentation: 海洋研究開発機構横浜研究所、横浜
  • Year and Date: 2007-07-07
• [Presentation] 繊毛虫における核退化:細胞死を伴わないユニークなアポトーシス (2007)
  • Author(s): 明松 隆彦、松岡 達臣、遠藤 浩
  • Organizer: 第3回日本進化原生生物学研究会
  • Place of Presentation: 海洋研究開発機構横浜研究所、横浜
  • Year and Date: 2007-07-07
• [Presentation] 中生動物ニハイチュウの寄生適応戦略:ミトコンドリア及び核,両オルガネラにおけるプログラム化されたゲノム再編成 (2007)
  • Author(s): 粟田 ひろ子、能登 朋子、遠藤 浩
  • Organizer: 第3回日本進化原生生物学研究会
  • Place of Presentation: 海洋研究開発機構 横浜研究所、横浜
  • Year and Date: 2007-07-07
• [Book] 生物学事典(共著(分担執筆)) (2010)
  • Publisher: 東京化学同人