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The functional analysis of microtubule-associated protein, Spag5, and the rescue experiment of hypogonadic phenotype by gene transfer of wild type Spag5 cDNA.

Research Project

Project/Area Number 19580350
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

SUZUKI Hiroetsu  Nippon Veterinary and Life Science University, 獣医学部, 准教授 (50277662)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords遺伝 / 不妊 / 精巣 / 細胞分裂 / Spag5 / Astrin / 精巣形成不全 / アポトーシス / 腎低形成 / 細胞増殖 / astrin
Research Abstract

Microtubule-associated protein Spag5 is believed to be required for the progression of mitotic metaphase. However, it has been reported that Spag5-knockout mice had normal phenotype. To reveal in vivo-function of Spag5, we made Spag5-transgenic rats, and mated them to the rats from hypogonadic (hgn) strain. The hgn/hgn phenotype is associated with spontaneous mutation of Spag5. Male hgn/hgn is infertile, and abnormal mitosis is found in Sertoli cells of their testes. The hgn/hgn males with Spag5 transgene showed normal testis and fertility. Therefore, Spag5 is required for normal testicular development.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 2007 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (22 results) Book (1 results)

  • [Journal Article] 日獣大オリジナルの自然発症性突然変異ラットを用いた病態モデルの開発と遺伝子機能の解析2009

    • Author(s)
      鈴木浩悦
    • Journal Title

      日本獣医生命科学大学研究報告 第58号

      Pages: 78-86

    • NAID

      80021272923

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the chromosomal inversion associated with the Koa mutation in the mouse revealed the cause of skeletal abnormalities.2009

    • Author(s)
      Katayama K, Miyamoto S, Furuno A, Akiyama K, Takahashi S, Suzuki H, Tsuji T, Kunieda T
    • Journal Title

      BMC genetics 10

      Pages: 60-60

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A spontaneous mutation of the Wwox gene and audiogenic seizure in rats with lethal dwarfism and epilepsy.2009

    • Author(s)
      Suzuki H, Katayama K, Motoo T, Amakasu K, Saito K, Suzuki K
    • Journal Title

      Genes, Brain and Behavior 8(7)

      Pages: 650-660

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Unilateral Urogenital Anomalies (UUA) rat: a new mutant strain associated with unilateral renal agenesis, cryptorchidism, and malformations of reproductive organs restricted in the left side.2009

    • Author(s)
      Amakasu K, Suzuki K, Suzuki H
    • Journal Title

      Comparative Medicine 59(3)

      Pages: 249-256

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A spontaneous mutation of the Wwox gene and audiogenic seizure in rats with lethal dwarfism and epilepsy.2009

    • Author(s)
      Hiroetsu Suzuki, et al
    • Journal Title

      Genes, Brain and Behavior 8

      Pages: 650-660

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Unilateral Urogenital Anomalies (UUA) rat : a new mutant strain associated with unilateral renal agenesis, cryptorchidism, and malformation of reproductive organs restricted in the left side.2009

    • Author(s)
      Kohei Amakasu, et al
    • Journal Title

      Comparative Medicine 59

      Pages: 249-256

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the chromosomal inversion associated with the Koa mutation in the mouse revealed the cause of skeletal abnormalities.2009

    • Author(s)
      Kentaro Katayama, et al
    • Journal Title

      BMC Genetics 10

      Pages: 60-60

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incomplete dominant osteochondrodysplasia in heterozygous Scottish Fold cats.2008

    • Author(s)
      Takanosu M, Takanosu T, Suzuki H, Suzuki K
    • Journal Title

      Journal of Small Animal Practice 49(4)

      Pages: 197-199

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The petit rat (pet/pet), a new semilethal mutant dwarf rat with thymic and testicular anomalies.2008

    • Author(s)
      Chiba J, Suzuki K, Suzuki H
    • Journal Title

      Comparative Medicine 58(6)

      Pages: 1-9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Retarded differentiation of Leydig cells and increased apoptosis of germ cells in the initial round of spermatogenesis of rats with lethal dwarf and epilepsy (lde/lde).2008

    • Author(s)
      Takenaka M, Yagi M, Suzuki K, Suzuki H
    • Journal Title

      Journal of Andrology 29

      Pages: 669-678

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Retarded differentiation of Leydig cells and increased apoptosis of germ cells in the initial round of spermatogenesis of rats with lethal dwarf and epilepsy (1de/de) phenotypes.2008

    • Author(s)
      Motoo Takenaka, et al
    • Journal Title

      Journal of Andrology 29

      Pages: 669-678

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The petit rat (pet/pet), a new semilethal mutant dwarf rat with thymic and testicular anomalies.2008

    • Author(s)
      Junko Chiba, et al
    • Journal Title

      Comparative. Medicine 58

      Pages: 551-559

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incomplete dominant osteochondrodysplasia in heterozygous Scottish Fold cats.2008

    • Author(s)
      Masamine Takanosu, et al
    • Journal Title

      Journal of Small Animal Practice 49

      Pages: 197-199

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced Mitotic Activity and Increased Apoptosis of Fetal Sertoli Cells in Rat Hypogonadic (hgn/hgn) Testes.2007

    • Author(s)
      Yagi M, Takenaka M, Suzuki K, Suzuki H
    • Journal Title

      Journal of Reproduction and Development 53(3)

      Pages: 581-589

    • NAID

      130000053889

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic characterization of spontaneously mutated rats showing lethal dwarfism and epilepsy.2007

    • Author(s)
      Suzuki H, Takenaka M, Suzuki K
    • Journal Title

      Comparative Medicine 57

      Pages: 360-369

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic pathogenesis of hypogonadism and renal hypoplasia in hgn/hgn rats characterized by male sterility, reduced female fertility, and progressive renal insufficiency.2007

    • Author(s)
      Suzuki H, Yagi M, Suzuki K
    • Journal Title

      Congenital Anomalies 47(1)

      Pages: 34-44

    • NAID

      10027386188

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic pathogenesis of hypogonadism and renal hypoplasia in hgn/hgn rats characterized by male sterility, reduced female fertility, and progressive renal insufficiency.2007

    • Author(s)
      Hiroetsu Suzuki, et. al.
    • Journal Title

      Congenital Anomalies 47

      Pages: 34-44

    • NAID

      10027386188

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced Mitotic Activity and Increased Apoptosis of Fetal Sertoli Cells in Rat Hypogonadic(hgn/hgn)Testes.2007

    • Author(s)
      Mio Yagi, et. al.
    • Journal Title

      Journal of Reproduction and Development 53

      Pages: 581-589

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic characterization of spontaneously mutated rats showing lethal dwarfism and epilepsy.2007

    • Author(s)
      Hiroetsu Suzuki, et. al.
    • Journal Title

      Comparative Medicine 57

      Pages: 360-369

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 精巣形成不全および腎低形成を呈するHGNラットのSpag5 cDNA導入(Spag5トランスジェニックラット)による救済実験2010

    • Author(s)
      片山健太郎
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] てんかん発作を伴う矮小ラット(lde/lde)のウエスタンブロット解析と脳病変異関する組織学的観察2010

    • Author(s)
      中井里香
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 両側性腎低形成症ラット(hgn/hgn)の変則的出による影響の評価2010

    • Author(s)
      茂木巡太郎
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] 両側性腎低形成症ラット(hgn/hgn)の片側腎摘出による影響の評価2010

    • Author(s)
      茂木巡太郎, 他
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] てんかん発作を伴う矮小ラット(lde/lde)のウエスタンブロット解析と脳病変に関する組織学的観察2010

    • Author(s)
      中井里香, 他
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 精巣形成不全および腎低形成を呈するHGNラットのSpag5 cDNA導入(Spag5トランスジェニック)による救済実験2010

    • Author(s)
      片山健太郎, 他
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨軟骨形成不全症(OCD)ラットはゴルジ体蛋白GiantinをコードするGolgb1遺伝子に挿入突然変異を持つ2009

    • Author(s)
      片山健太郎
    • Organizer
      日本動物遺伝育種学会第10回記念大会
    • Place of Presentation
      群馬会館
    • Year and Date
      2009-11-09
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨軟骨形成不全症(OCD)ラットはゴルジ体タンパクGiantinをコードするGolgbl遺伝子に挿入突然変異を持つ2009

    • Author(s)
      片山健太郎, 他
    • Organizer
      日本動物遺伝育種学会第10回記念大会
    • Place of Presentation
      群馬会館
    • Year and Date
      2009-11-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨軟骨形成不全症(OCD)ラットの胎生期における大腿骨の病理解析2009

    • Author(s)
      後藤匠
    • Organizer
      第148回日本獣医学会学術集会
    • Place of Presentation
      鳥取市民会館
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 精巣形成不全および両側性腎低形成症を呈するラット(hgn/hgn)の骨形態計測の所見2009

    • Author(s)
      稲垣将治
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2009-04-04
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] てんかん発作を伴う矮小ラット(lde/lde)の原因遺伝子はWWOXである2009

    • Author(s)
      鈴木浩悦
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2009-04-04
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 骨軟骨形成不全症(OCD)ラットのGolgbl遺伝子における10bpの挿入突然変異の同定2009

    • Author(s)
      片山健太郎, 他
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2009-04-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 先天性片側精巣下降不全ラットの精巣についての内分泌学的検討2008

    • Author(s)
      甘粕晃平
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      ワールドコンベンショナルサミット(シーガイヤ)
    • Year and Date
      2008-09-24
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] てんかんを伴う致死性の矮小ラットにおける精巣病態と遺伝解析2008

    • Author(s)
      鈴木浩悦
    • Organizer
      日本アンドロロジー学会第27回学術集会
    • Place of Presentation
      先斗町歌舞練場
    • Year and Date
      2008-07-04
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 先天性片側性腎欠損ラットの残存腎における細胞増殖活性の評価2008

    • Author(s)
      甘粕晃平
    • Organizer
      第145回日本獣医学会学術集会
    • Place of Presentation
      麻布大学
    • Year and Date
      2008-03-30
    • Related Report
      2009 Final Research Report
  • [Presentation] 先天性片側性腎欠損ラットの残存腎における細胞増殖活性の評価2008

    • Author(s)
      甘粕晃平、他
    • Organizer
      第145回獣医学会
    • Place of Presentation
      麻布大学
    • Year and Date
      2008-03-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Theodur経口投与によるマウス脳波の変化2007

    • Author(s)
      斉藤賢一
    • Organizer
      日本てんかん学会第41回大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-11-02
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 電磁波が胎生期マウスの発生に与える影響の研究、第2報2007

    • Author(s)
      斉藤賢一
    • Organizer
      日本先天異常学会第47回大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-07
    • Related Report
      2009 Final Research Report
  • [Presentation] てんかんモデル動物(Elマウス)におけるValproate投与によるinterictal脳波の変化2007

    • Author(s)
      斉藤賢一
    • Organizer
      日本先天異常学会第47回大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-07
    • Related Report
      2009 Final Research Report
  • [Presentation] 癲癇モデル動物(Elマウス)におけるValproate投与によるinterictal脳波の変化2007

    • Author(s)
      斉藤賢一、他
    • Organizer
      日本先天異常学会第47回大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] 電磁波が胎生期マウスの発生に与える影響の研究 第2報2007

    • Author(s)
      宮原晋一、他
    • Organizer
      日本先天異常学会第47回大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-07-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] 骨軟骨形成不全症(OCD)ラットのGolgb1遺伝子における10bpの挿入突然変異の同定

    • Author(s)
      片山健太, 等
    • Related Report
      2009 Final Research Report
  • [Book] 食品安全ハンドブック, 分担:コナゾール2009

    • Author(s)
      林裕造, 大野泰雄編集
    • Publisher
      丸善
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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