Analysis of target antigens of anti-cell surface antibodies in patients with collagen diseases and development of clinical assay

• Project/Area Number: 19591188
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Fujita Health University
• Principal Investigator: MIURA Keiji Fujita Health University, 総合医科学研究所, 助教 (20199946)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膠原病 / 自己抗体 / 自己抗原 / 細胞表面抗原 / 膜タンパク質 / 可溶化 / ELISA / 診断法

Research Abstract

Sera from 72 patients with collagen diseases were tested for antibodies against cell surface antigens by Cyto-ELISA with different cells as antigenic substrates. ELISA test with solubilized membrane fraction was developed for systemic lupus erythematosus and systemic sclerosis.