Application of Monolithic Porous Layer on SiWafer -Toward Cell Manipulation for Biotechnology-

• Project/Area Number: 19686014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Production engineering/Processing studies
• Research Institution: Tokyo University of Science
• Principal Investigator: 早瀬 仁則 Tokyo University of Science, 理工学部, 准教授 (70293058)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
• Keywords: MEMS / 多孔質シリコン / 細胞アレイ / 微細加工 / ナノバイオ / マイクロ・ナノデバイス / 細胞固定 / 徴細加工

Research Abstract

Through-chip porous area array was made on a Si chip and cellular array was demonstrated by vacuum suction on the through-chip porous areas. It was expected that the porous area was easily blocked by some substances contained in a cultivation medium. Then the pressure drop through the porous area was measured and it was found that enough suctional fluidic volume can be obtained with a fresh filtrated cultivation medium. Cell immobilization was demonstrated and it was found that cell fragmentation could be induced after the immobilization by applying strong suction.

Research Products

• [Journal Article] Formation of Porous Noble Metal Layer by Displacement Reactions with Porous Silicon (2008)
  • Author(s): M. Hayase, T. Matsuzaka, J.G.A. Brito-Neto
  • Journal Title: ECS Transactions 16(3) Pages: 231-235
• [Journal Article] 貫通多孔質シリコン層を用いた微小物体吸引デバイスの開発 (2008)
  • Author(s): 早瀬仁則, 加藤厚子, 内海敦
  • Journal Title: 精密工学会誌 74(10) Pages: 1056-1061
  • NAID: 110006951283
  • Peer Reviewed
• [Journal Article] Porous Gold Structures Templated by Porous Silicon (2008)
  • Author(s): J.G.A. Brito-Neto, K. Kondo, M. Hayase
  • Journal Title: Journal of The Electrochemical Society 155(1)
  • Peer Reviewed
• [Journal Article] 貫通多孔質シリコン層を用いた微小物体吸引デバイスの開発 (2008)
  • Author(s): 早瀬仁則, 加藤厚子, 内海敦
  • Journal Title: 精密工学会誌 74 Pages: 1056-1061
  • NAID: 110006951283
  • Peer Reviewed
• [Presentation] Formation of Through-Chip Porous Noble MetalLayers on Silicon Substrates (2009)
  • Author(s): T. Matsuzaka, J. Shimanuki, T. Ishiguro, M. Hayase
  • Organizer: 216th Meeting of the Electrochemical Society
  • Place of Presentation: Austria
  • Year and Date: 2009-11-06
• [Presentation] Formation of Through-chip Porous Metal Layer on Si Substrates (2009)
  • Author(s): Taku Matsuzaka
  • Organizer: 216^<th> The Electrochemical Society Meeting
  • Place of Presentation: ウィーン(オーストリア)
  • Year and Date: 2009-10-06
• [Presentation] シリコン基板上への貫通した多孔質貴金属層の形成 (2009)
  • Author(s): 松坂拓
  • Organizer: 精密工学会秋季学術講演会
  • Place of Presentation: 神戸大学
  • Year and Date: 2009-09-12
• [Presentation] シリコン基板上への貫通した多孔質貴金属層の形成 (2009)
  • Author(s): 松坂拓, 島貫純一, 早瀬仁則
  • Organizer: 2009年度精密工学会秋季大会学術講演会
  • Place of Presentation: 神戸大学
  • Year and Date: 2009-09-10
• [Presentation] Porous Silicon Based Microfluidic Device for Bio-applications (2008)
  • Author(s): V.N. Tondare, N. Ozawa, M. Hayase
  • Organizer: 2008年度精密工学会秋季大会学術講演会
  • Place of Presentation: 東北大学
  • Year and Date: 2008-09-18
• [Presentation] A novel device for fixing small objects using through-pore porous silicon (2007)
  • Author(s): K. Kato, A. Uchiumi, M. Hayase
  • Organizer: TRANSDUCES 2007
  • Place of Presentation: France
  • Year and Date: 2007-06-14
• [Presentation] A Novel Device for Fixing Small Objects Using Through-Pore Porous Silicon (2007)
  • Author(s): Kato, A.
  • Organizer: TRANSDUCERS'07
  • Place of Presentation: リヨン,フランス
  • Year and Date: 2007-06-12
• [Book] 細胞分離・操作技術の最前線 (2008)
  • Author(s): 早瀬仁則
  • Publisher: シーエムシー出版
• [Book] 第4章 微細孔吸引による細胞固定技術(細胞分離・操作技術の最前線) (2008)
  • Author(s): 早瀬 仁則(監修: 福田, 新井)
  • Total Pages: 387