Studies on the activation volume of an incorporation process by in situ measurements of step velocities under high pressure

• Project/Area Number: 19760009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied materials science/Crystal engineering
• Research Institution: The University of Tokushima
• Principal Investigator: SUZUKI Yoshihisa The University of Tokushima, 大学院・ソシオテクノサイエンス研究部, 講師 (60325248)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 結晶成長 / タンパク質 / 圧力 / 核生成 / glucose isomerase / 構造解析 / タンバク質

Research Abstract

Effects of pressure on the step kinetic coefficients of glucose isomerase crystals were determined by in situ observation of growing step of the crystals under high pressure. An activation volume of glucose isomerase crystals took a negative value.

Research Products

• [Journal Article] Effects of High Pressure on the Three-Dimensional Nucleation Rates of Glucose Isomerase Crystals (2010)
  • Author(s): Takeshi Maruoka, Yoshihisa Suzuki, Katsuhiro Tamura
  • Journal Title: Journal of Physics Conference Series Vol.215 Pages: 12158-12158
  • Peer Reviewed
• [Journal Article] Solubility measurements of protein crystals under high pressure by in situ observation of steps on crystal surfaces (2010)
  • Author(s): Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Katsuhiro Tamura
  • Journal Title: Journal of Physics Conference Series Vol.215 Pages: 12159-12159
  • Peer Reviewed
• [Journal Article] First Direct Observation of Elementary Steps on the Surfaces of Glucose Isomerase Crystals under High Pressure (2009)
  • Author(s): Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima, Katsuhiro Tamura
  • Journal Title: Crystal Growth & Design Vol.9 Pages: 4289-4289
  • Peer Reviewed
• [Journal Article] First Direct Observation of Elementary Steps on the Surfaces of Glucose Isomerase Crystals under Hieh Pressure (2009)
  • Author(s): Yoshihisa Suzuki
  • Journal Title: Crystal Growth & Design 9 Pages: 4289-4295
  • Peer Reviewed
• [Journal Article] 高圧力下で成長したグルコースイソメラーゼ結晶の常圧下でのX線結晶構造解析 (2008)
  • Author(s): 塚本雅之, 鈴木良尚, 櫻庭春彦, 田村 勝弘
  • Journal Title: 高圧バイオサイエンスとバイオテクノロジー第15回生物関連高圧研究会20周年記念シンポジウム抄録集 Vol.2 Pages: 29-29
• [Journal Article] 高圧力下で成長したグルコースイソメラーゼ結晶の常圧下でのX線結晶構造解析 (2008)
  • Author(s): 塚本雅之
  • Journal Title: 高圧バイオサイエンスとバイオテクノロジー第15回生物関連高圧研究会20周年記念シンポジウム抄録集 2 Pages: 29-37
  • Peer Reviewed
• [Presentation] グルコースイソメラーゼ結晶の結晶化素過程における活性化体積 (2009)
  • Author(s): 鈴木良尚, 佐崎元, 柳谷伸一郎, 田村 勝弘
  • Organizer: NCCG-39予稿集
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-13
• [Presentation] グルコースイソメラーゼ結晶の結晶化素過程における活性化体積 (2009)
  • Author(s): 鈴木良尚
  • Organizer: 第39回結晶成長国内会議
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2009-11-13
• [Presentation] リゾチーム正方晶系結晶の外形変化とステップ速度から求めた溶解度の比較 (2009)
  • Author(s): 藤原貴久, 鈴木良尚, 森篤史, 柳谷伸一郎, 田村勝弘
  • Organizer: NCCG-39予稿集
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-12
• [Presentation] リゾチーム正方晶系結晶の外形変化とステップ速度から求めた溶解度の比較 (2009)
  • Author(s): 藤原貴久
  • Organizer: 第39回結晶成長国内会議
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2009-11-12
• [Presentation] Effects of High Pressure on the Three-Dimensional Nucleation Rates of Glucose Isomerase Crystals (2009)
  • Author(s): Takeshi Maruoka, Yoshihisa Suzuki, Katsuhiro Tamura
  • Organizer: International Conference on High Pressure Science and Technology AIRAPT-22
  • Place of Presentation: 東京
  • Year and Date: 2009-07-30
• [Presentation] Effects of High Pressure on the Three-Dimensional Nucleation Rates of Glucose Isomerase Crystals (2009)
  • Author(s): Maruoka Takeshi
  • Organizer: International Conference on High Pressure Science and Technology AIRAPT-22
  • Place of Presentation: 東京国際交流館(東京)
  • Year and Date: 2009-07-30
• [Presentation] Solubility measurements of protein crystals under high pressure by in situ observation of steps on crystal surfaces (2009)
  • Author(s): Takahisa Fujiwara, Yoshihisa Suzuki, Gen Sazaki, Katsuhiro Tamura
  • Organizer: International Conference on High Pressure Science and Technology AIRAPT-22
  • Place of Presentation: 東京
  • Year and Date: 2009-07-29
• [Presentation] Solubility measurements of protein crystals under high pressure by in situ observation of steps on crystal surfaces (2009)
  • Author(s): Takahisa Fujiwara
  • Organizer: International Conference on High Pressure Science and Technology AIRAPT-22
  • Place of Presentation: 東京国際交流館(東京)
  • Year and Date: 2009-07-29
• [Presentation] 正方晶系リゾチーム結晶のステップ前進速度に及ぼすpHの効果 (2008)
  • Author(s): 内田直祐, 鈴木良尚, 田村勝弘
  • Organizer: 2008日本化学会西日本大会
  • Place of Presentation: 長崎
  • Year and Date: 2008-11-15
• [Presentation] 正方晶系リゾチーム結晶のステヅプ前進速度に及ぼすpHの効果 (2008)
  • Author(s): 内田直祐
  • Organizer: 2008日本化学会西日本大会
  • Place of Presentation: 長崎
  • Year and Date: 2008-11-15
• [Presentation] 共焦点微分干渉顕微鏡を用いたリゾチーム単斜晶系結晶表面のステップ前進速度の測定 (2008)
  • Author(s): 田中孝典, 鈴木良尚, 田村勝弘
  • Organizer: 第38回結晶成長国内会議
  • Place of Presentation: 仙台
  • Year and Date: 2008-11-04
• [Presentation] リゾチーム正方晶系結晶の外形・表面のその場観察による溶解度測定法 (2008)
  • Author(s): 藤原貴久, 鈴木良尚, 田村勝弘
  • Organizer: 第38回結晶成長国内会議
  • Place of Presentation: 仙台
  • Year and Date: 2008-11-04
• [Presentation] 共焦点微分干渉顕微鏡を用いたリゾチーム単斜晶系結晶表面のステヅプ前進速度の測定 (2008)
  • Author(s): 田中孝典
  • Organizer: 第38回結晶成長国内会議
  • Place of Presentation: 仙台
  • Year and Date: 2008-11-04
• [Presentation] Effects of pressure on the step velocity, two-dimensional nucleation rate and solubility of glucose isomerase crystals (2008)
  • Author(s): Yoshihisa Suzuki, Gen Sazaki, Masamitsu Matsumoto, Makoto Nagasawa, Kazuo Nakajima, Katsuhiro Tamura
  • Organizer: 5th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: San Diego
  • Year and Date: 2008-09-17
• [Presentation] Effects of pressure on the step velocity two-dimensional nucleation rate and solubility of glucose isomerase crystals (2008)
  • Author(s): Yoshihisa Suzuki
  • Organizer: 5th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: San Diego
  • Year and Date: 2008-09-17
• [Presentation] Solubility measurements by in situ observation of the steps of tetragonal lysozyme crystals under high pressure (2008)
  • Author(s): Takahisa Fujiwara, Yoshihisa Suzuki, Katsuhiro Tamura
  • Organizer: 5th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: San Diego
  • Year and Date: 2008-09-16
• [Presentation] X-ray structure analysis of glucose isomerase crystals prepared under high pressure (2008)
  • Author(s): Masayuki Tsukamoto, Yoshihisa Suzuki, Haruhiko Sakuraba, Katsuhiro Tamura
  • Organizer: Program and abstract book 5th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: San Diego
  • Year and Date: 2008-09-16
• [Presentation] X-ray structure analysis of glucose isomerase crystals prepared under high pressure (2008)
  • Author(s): Masayuki Tsukamoto
  • Organizer: 5th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: San Diegc
  • Year and Date: 2008-09-16
• [Presentation] リゾチーム正方晶系結晶の三面角付近の観察による溶解度測定 (2007)
  • Author(s): 藤原貴久, 鈴木良尚, 田村勝 弘
  • Organizer: 第37回結晶成長国内会議
  • Place of Presentation: 札幌
  • Year and Date: 2007-11-06
• [Presentation] 高圧力下で成長したグルコースイソメラーゼ結晶の常圧下でのX線結晶構造解析 (2007)
  • Author(s): 塚本雅之, 鈴木良尚, 櫻庭春彦, 田村勝弘
  • Organizer: 第37回結晶成長国内会議
  • Place of Presentation: 札幌
  • Year and Date: 2007-11-05
• [Presentation] 位相差顕微鏡によるグルコースイソメラーゼ結晶表面の分子ステップ観察 (2007)
  • Author(s): 鈴木良尚, 佐崎元, 田村勝弘
  • Organizer: 第15回生物関連高圧研究会20周年記念シンポジウム
  • Place of Presentation: 横須賀
  • Year and Date: 2007-09-07
• [Presentation] 位相差顕微鏡によるグルコースイソメラーゼ結晶表面の分子ステップ観察 (2007)
  • Author(s): 鈴木良尚
  • Organizer: 第19回生物関連高圧研究会20周年記念シンポジウム
  • Place of Presentation: 横須賀
  • Year and Date: 2007-09-07
• [Presentation] グルコースイソメラーゼの三次元分子構造に及ぼす圧力効果 (2007)
  • Author(s): 塚本雅之, 鈴木良尚, 櫻庭春彦, 田村勝弘
  • Organizer: 第15回生物関連高圧研究会20周年記念シンポジウム
  • Place of Presentation: 横須賀
  • Year and Date: 2007-09-06
• [Presentation] グルコースイソメラーゼの三次元分子構造に及ぼす圧力効果 (2007)
  • Author(s): 塚本雅之
  • Organizer: 第19回生物関連高圧研究会20周年記念シンポジウム
  • Place of Presentation: 横須賀
  • Year and Date: 2007-09-06
• [Remarks]
  • URL: http://pub2.db.tokushima-u.ac.jp/ERD/person/10688/profile-ja.html
• [Remarks]
  • URL: http://pub2.db.tokushima-u.ac.jp/ERD/person/10688/profile-ja.html