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Understanding of wood decay phenomena and construction of biomass conversion system using genome editing technology of mushroom

Research Project

Project/Area Number 19H03013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Igarashi Kiyohiko  東京大学, 大学院農学生命科学研究科(農学部), 教授 (80345181)

Co-Investigator(Kenkyū-buntansha) 飛松 裕基  京都大学, 生存圏研究所, 准教授 (20734221)
高畠 幸司  琉球大学, 農学部, 教授 (50446621)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords木材腐朽菌 / きのこ / ゲノム編集 / 薬剤耐性 / バイオマス変換 / バイオエコノミー / 二次元NMR
Outline of Research at the Start

きのこの多くは、木材を栄養源として育つ「木材腐朽菌」に属する糸状菌であり、エノキタケやシイタケのような食品として認識されているのは、それらの子実体と呼ばれる部分である。きのこ生産の市場規模は年間2,000億円程度であるが、これは(木材や紙生産を含めた)全林産物の市場規模の約半分、特用林産物(きのこや炭、栗など)の市場の約9割を占める重要な産業であると同時に、木質バイオマスを利用して純利益を生み出しているバイオマス利用の鏡とも言える産業である。そこで本研究ではきのこのゲノム編集技術を利用して「きのこはどのように木材を分解しているのか」を明らかにする事で、木質バイオマスの高度利用のヒントを得る。

Outline of Final Research Achievements

Most mushrooms are wood-rotting fungi that grow on wood as a nutrient source. Although the fruiting bodies produced by mushrooms have been used as food and medicine since ancient times, the mechanisms of wood decomposition and fruiting body formation remain largely unknown. The purpose of this study is to understand the wood rot phenomenon using genome editing technology and to apply it to the construction of a new biomass conversion system. In this study, we succeeded in obtaining comprehensive data on antibiotic resistance of wood-rotting fungi, for which only scattered information had been available so far. We have also succeeded in mutating enzymes related to biomass degradation in the basidiomycete Coprinopsis cinerea, suggesting that we were able to acquire experimental techniques that lead to a better understanding of wood decay phenomena.

Academic Significance and Societal Importance of the Research Achievements

本研究では、木材の物質変換を司っている木材腐朽菌(きのこ)にゲノム編集技術を応用し、木質バイオマス新しいバイオマス変換系の構築を試みた。木質バイオマスを前処理無しで糖化できる技術開発や、セルロースナノファイバーなどの高付加価値な素材を木材から低エネルギーで生産する技術開発を行い、それをきのこ生産現場で展開することによって林業や林産業への雇用創出や気候変動への貢献につながることが期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (19 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Remarks (2 results)

  • [Int'l Joint Research] ケンブリッジ大学(英国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] スウェーデン農業科学大学/ウプサラ大学(スウェーデン)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Acetylated Xylan Degradation by Glycoside Hydrolase Family 10 and 11 Xylanases from the White-rot Fungus <i>Phanerochaete chrysosporium</i>2022

    • Author(s)
      Kojima Keisuke、Sunagawa Naoki、Yoshimi Yoshihisa、Tryfona Theodora、Samejima Masahiro、Dupree Paul、Igarashi Kiyohiko
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 69 Issue: 2 Pages: 35-43

    • DOI

      10.5458/jag.jag.JAG-2021_0017

    • ISSN
      1344-7882, 1880-7291
    • Year and Date
      2022-05-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface2022

    • Author(s)
      Uchiyama Taku、Uchihashi Takayuki、Ishida Takuya、Nakamura Akihiko、Vermaas Josh V.、Crowley Michael F.、Samejima Masahiro、Beckham Gregg T.、Igarashi Kiyohiko
    • Journal Title

      Science Advances

      Volume: 8 Issue: 51

    • DOI

      10.1126/sciadv.ade5155

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison of glycoside hydrolase family 3 β-xylosidases from basidiomycetes and ascomycetes reveals evolutionarily distinct xylan degradation systems2022

    • Author(s)
      Kojima Keisuke、Sunagawa Naoki、Mikkelsen Nils Egil、Hansson Henrik、Karkehabadi Saeid、Samejima Masahiro、Sandgren Mats、Igarashi Kiyohiko
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Issue: 3 Pages: 101670-101670

    • DOI

      10.1016/j.jbc.2022.101670

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of C-6 Methylol Groups on Substrate Recognition of Glucose/Xylose Mixed Oligosaccharides by Cellobiose Dehydrogenase from the Basidiomycete <i>Phanerochaete chrysosporium</i>2020

    • Author(s)
      Igarashi, K., Kaneko, S., Kitaoka, M., and Samejima, M.
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 67 Issue: 2 Pages: 51-57

    • DOI

      10.5458/jag.jag.JAG-2020_0003

    • NAID

      130007844422

    • ISSN
      1344-7882, 1880-7291
    • Year and Date
      2020-05-20
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unique active-site and subsite features in the arabinogalactan-degrading GH43 exo-β-1,3-galactanase from Phanerochaete chrysosporium2020

    • Author(s)
      Matsuyama Kaori、Kishine Naomi、Fujimoto Zui、Sunagawa Naoki、Kotake Toshihisa、Tsumuraya Yoichi、Samejima Masahiro、Igarashi Kiyohiko、Kaneko Satoshi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Issue: 52 Pages: 18539-18552

    • DOI

      10.1074/jbc.ra120.016149

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermostable Mutants of Glycoside Hydrolase Family 6 Cellobiohydrolase from the Basidiomycete <i>Phanerochaete chrysosporium</i>2020

    • Author(s)
      Yamaguchi Sora、Sunagawa Naoki、Tachioka Mikako、Igarashi Kiyohiko、Samejima Masahiro
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 67 Issue: 3 Pages: 79-86

    • DOI

      10.5458/jag.jag.jag-2020_0004

    • NAID

      130007896077

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single amino Acid mutation of pyranose 2-oxidase results in increased specificity for diabetes biomarker 1,5-anhydro-D-glucitol2020

    • Author(s)
      Fujii, T. Igarashi, K., and Samejima, M.
    • Journal Title

      J. Appl. Glycosci.

      Volume: 67 Issue: 3 Pages: 73-78

    • DOI

      10.5458/jag.jag.jag-2020_0002

    • NAID

      130007896067

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Nordic Polymer Conference/Nordic Polymer Days 20212021

    • Author(s)
      Kiyohiko Igarashi
    • Organizer
      Enzymatic degradation and synthesis of crystalline cellulose
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] セルロース分解酵素の中性子構造と古くて新しい加水分解反応メカニズム2021

    • Author(s)
      五十嵐 圭日子
    • Organizer
      iBIXタンパク質構造研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 植物細胞壁の生分解と生合成を模した循環型もの作り2021

    • Author(s)
      五十嵐 圭日子
    • Organizer
      第38回日本植物バイオテクノロジー学会(つくば)大会シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Breaking and making cellulose by enzymes: Perspective on biomass research in Japan and Scandinavia2021

    • Author(s)
      Kiyohiko Igarashi
    • Organizer
      JSPS-ACD hybrid seminar: Biosustainable Chemistry from Biomass
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 次世代バイオマス変換技術:サーキュラーバイオエコノミー実現のためにいかに酵素を利用するか2021

    • Author(s)
      五十嵐 圭日子
    • Organizer
      神戸大ワークショップ2021
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 担子菌Coprinopsis cinereaにおけるゲノム編集を用いた糖質関連酵素ノックアウト株の作出2020

    • Author(s)
      木根啓太,五十嵐圭日子,大霜晶子,刑部祐里子,刑部敬史
    • Organizer
      第70回木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 糸状菌が生産するバイオマス分解酵素の分子から原子レベル解析2019

    • Author(s)
      五十嵐圭日子
    • Organizer
      植物バイテク談話会特別セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 微生物の力を借りて「機構崩壊」を止める:バイオエコノミー2019

    • Author(s)
      五十嵐圭日子
    • Organizer
      SEEDS Conference 2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] キノコとカビにおけるヘミセルロース分解戦略の微妙な違い

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20220225-1.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 枝をよけながら幹を切る:複雑な糖鎖を分解できる酵素のユニークな構造

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20201027-2.html

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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