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Radioresponce evaluation by using cancer stem cell in PDX mouse

Research Project

Project/Area Number 19K17169
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 52040:Radiological sciences-related
Research InstitutionKobe University

Principal Investigator

Mohamed Mohamed  神戸大学, 医学研究科, 外国人研究員 (00833378)

Project Period (FY) 2019-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsPDXマウス / 放射線増感 / がん / がん幹細胞 / 頭頸部がん / Radiation response / cancer stem cell / PDX mouse
Outline of Research at the Start

我々は、患者個別のがん幹細胞を確認し、前臨床試験モデルとして、患者由来のがん組織をマウスで増殖させるPDXモデルを作製に成功した。このPDX腫瘍モデルは、患者由来の新鮮な腫瘍組織を用いて、従来のがん細胞株をヌードマウスに移植した動物モデルでとは全く異なる特徴を持ち、より実臨床に近い重要な情報を検討する。本研究では、頭頸部がん症例からの腫瘍組織を採取し、放射線感受性と、ヒトがん免疫応答を再現できる新規動物実験モデルを開発し、がん幹細胞や、その他の免疫応答に関連する新たな腫瘍抗原の探索など新規の放射線免疫療法の開発に取り組む。

Outline of Final Research Achievements

Methods to predict radiation effects remain undetermined. We engaged to establish
patient-Derived Xenograft (PDX) mouse models using patients samples. The PDX mouse model shows quite different characteristics from xenografts using nude mice, and the PDX mouse model express more similar to patients conditions. We injected breast cancer or colon cancer samples to NOD/SID/IL2rgKO (NSG) mice. Using these models, we have challenged to evaluate radiation effects in the NSG mice models.

Academic Significance and Societal Importance of the Research Achievements

放射線治療の効果を治療前に予測することが可能になれば、がん治療の治療選択にも役立ち、より低侵襲な治療を提供することが可能である。しかしながら現況では、組織型や文化度など、僅かな判断基準があるだけで実際には効果予測は難しい。近年登場したPDXマウスモデルは患者の組織を直接NSGマウスモデルを用いて直接再現できるため、新たな診断ツールになりえると思われる。我々は乳がんや大腸がんではそのモデルの作成に成功し、放射線効果を判定することを試みた。今後さらに多種類のがん組織を用いて、本システムが実用可能かを判定していく。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Exosome-mediated radiosensitizing effect on neighboring cancer cells via increase in intracellular levels of reactive oxygen species2021

    • Author(s)
      Nakaoka Ai、Nakahana Makiko、Inubushi Sachiko、Akasaka Hiroaki、Salah Mohammed、Fujita Yoshiko、Kubota Hikaru、Hassan Mennaallah、Nishikawa Ryo、Mukumoto Naritoshi、Ishihara Takeaki、Miyawaki Daisuke、Sasayama Takashi、Sasaki Ryohei
    • Journal Title

      Oncology Reports

      Volume: 45 Issue: 4 Pages: 1-1

    • DOI

      10.3892/or.2021.7964

    • NAID

      120006975755

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Elucidation of gastrointestinal dysfunction in response to irradiation using metabolomics2020

    • Author(s)
      Salah Mohammed、Osuga Saki、Nakahana Makiko、Irino Yasuhiro、Shinohara Masakazu、Shimizu Yasuyuki、Mukumoto Naritoshi、Akasaka Hiroaki、Nakaoka Ai、Miyawaki Daisuke、Ishihara Takeaki、Yoshida Kenji、Okamoto Yoshiaki、Sasaki Ryohei
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 23 Pages: 100789-100789

    • DOI

      10.1016/j.bbrep.2020.100789

    • NAID

      120007041619

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Comparative Assessment of Mechanisms and Effectiveness of Radiosensitization by Titanium Peroxide and Gold Nanoparticles2020

    • Author(s)
      Hassan Mennaallah、Nakayama Masao、Salah Mohammed、Akasaka Hiroaki、Kubota Hikaru、Nakahana Makiko、Tagawa Tatsuichiro、Morita Kenta、Nakaoka Ai、Ishihara Takeaki、Miyawaki Daisuke、Yoshida Kenji、Nishimura Yuya、Ogino Chiaki、Sasaki Ryohei
    • Journal Title

      Nanomaterials

      Volume: 10 Issue: 6 Pages: 1125-1125

    • DOI

      10.3390/nano10061125

    • NAID

      120006878579

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Radiosensitive Effect induced by exosomes from irradiated cancer cells2021

    • Author(s)
      Ai Nakaoka, Makiko Nakahana, Naritoshi Mukumoto, Hiroaki Akasaka, Mohammed Salah, Ryo Nishikawa, Takeaki Ishihara, Daisuke Miyawaki, Takashi Sasayama and Ryohei Sasaki*
    • Organizer
      The 4 th International Cancer Research Symposium a Training for Oncology Professionals
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] エクソソームを介した腫瘍細胞への放射線増感効果2020

    • Author(s)
      中岡 藍、仲波名 真希子、椋本 成俊、赤坂 浩亮、清水 康之、Mohammed Salah、石原 武明 、宮脇 大輔、佐々木 良平
    • Organizer
      第63回日本放射線影響学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Titanium peroxide nanoparticle enhance the efficacy of immuno-radiotherapy via activated CD8+ T cell dependent antitumor effect in vivo2020

    • Author(s)
      Hikaru Kubota, Yoshiko Fujita, Mohammed Salah, Yasuyuki Shimizu, Hiroaki Akasaka, Ryohei Sasaki
    • Organizer
      American Society for Radiation Oncology 62nd Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Radiosensitization by metal nanoparticles: A comparison of titanium peroxide nanoparticles with gold nanoparticles2019

    • Author(s)
      Mennaallah Alsayed, Masao Nakayama, Hiroaki Akasaka, Mohammed Salah, Makiko Nakahana, Kenta Morita, Naritoshi Mukumoto, Chiaki Ogino, Takeaki Ishihara and Ryohei Sasaki
    • Organizer
      The 7th Japan-Taiwan Radiation Oncology Symposium
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2022-01-27  

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