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Induction of nitrogen-fixing root nodules in rice

Research Project

Project/Area Number 19K22303
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Okazaki Shin  東京農工大学, (連合)農学研究科(研究院), 教授 (40379285)

Project Period (FY) 2019-06-28 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords根粒 / イネ / 窒素固定 / 根粒菌 / エフェクター
Outline of Research at the Start

根粒菌はマメ科植物に根粒を形成し、窒素固定することで宿主植物に窒素栄養を供給することができる。窒素肥料の大幅な使用削減を実現するための手段として、イネ科植物に窒素固定根粒を形成させる研究が世界中で進行中であるが、未だ成功には至っていない。申請者らは一部の根粒菌がエフェクタータンパク質をマメ科植物細胞に注入し、根粒形成シグナルを直接起動することを発見した。本研究では、宿主の根粒形成シグナルを直接起動する根粒菌を利用して、世界で初めてイネ科植物に窒素固定根粒を形成させることを目指す。

Outline of Final Research Achievements

In this study, we attempted to induce the formation of nitrogen-fixing nodules in rice using rhizobia that secretes nodulation-inducing proteins (effectors) and substances that form nodule-like tissues in rice roots. As a result, we revealed that, (1) rhizobium colonized rice roots and can express effector genes by adding genistein at the same time, and (2) addition of 2,4-D enhanced cell division in rice roots. (3) Rhizobium colonized on the surface of these nodule-like structures, and simultaneous addition of genistein increased the amount of rhizobium colonization. However, we could not observe invasion of rhizobia into the nodule-like structures, and could not observe the expression of nitrogen-fixing genes.

Academic Significance and Societal Importance of the Research Achievements

現代農業で最も多く利用される窒素肥料は、水質汚染や温室効果ガス排出など生態系に悪影響を及ぼす。一方、根粒菌はマメ科植物の根に根粒を形成し、窒素固定により宿主植物に窒素栄養を供給する。本研究ではイネ科植物への窒素固定根粒形成を試みた。結果として、2,4-Dにより人為的にイネ根に膨らみを形成できること、ゲニステインにより根粒菌のイネ根への定着を向上できることを明らかにした。これまでのところイネ根に形成された膨らみに根粒菌を侵入・感染するところまでは確認できていないため、今後、根粒菌の侵入・感染と窒素固定遺伝子発現を可能にする因子を根粒菌側、イネ側両方から解析することが必要と考える。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] フランスの農業開発国際研究センター(フランス)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] IRD(フランス国立開発研究所)(フランス)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Type III effector provides a novel symbiotic pathway in legume?rhizobia symbiosis2022

    • Author(s)
      Ratu Safirah Tasa Nerves、Amelia Lidia、Okazaki Shin
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 87 Issue: 1 Pages: 28-37

    • DOI

      10.1093/bbb/zbac178

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple Domains in the Rhizobial Type III Effector Bel2-5 Determine Symbiotic Efficiency With Soybean2021

    • Author(s)
      Ratu Safirah Tasa Nerves、Hirata Atsushi、Kalaw Christian Oliver、Yasuda Michiko、Tabuchi Mitsuaki、Okazaki Shin
    • Journal Title

      Frontiers in Plant Science

      Volume: 12

    • DOI

      10.3389/fpls.2021.689064

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rhizobia use a pathogenic-like effector to hijack leguminous nodulation signalling2021

    • Author(s)
      Ratu Safirah Tasa Nerves、Teulet Albin、Miwa Hiroki、Masuda Sachiko、Nguyen Hien P.、Yasuda Michiko、Sato Shusei、Kaneko Takakazu、Hayashi Makoto、Giraud Eric、Okazaki Shin
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 2034-2034

    • DOI

      10.1038/s41598-021-81598-6

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of Bradyrhizobium elkanii USDA61 Type III Effectors Determining Symbiosis with Vigna mungo2020

    • Author(s)
      Nguyen Hien P.、Ratu Safirah T. N.、Yasuda Michiko、Teaumroong Neung、Okazaki Shin
    • Journal Title

      Genes

      Volume: 11 Issue: 5 Pages: 474-474

    • DOI

      10.3390/genes11050474

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The rhizobial type III effector ErnA confers the ability to form nodules in legumes2019

    • Author(s)
      Teulet Albin、Busset Nicolas、Fardoux Joel、Gully Djamel、Chaintreuil Clemence、Cartieaux Fabienne、Jauneau Alain、Comorge Virginie、Okazaki Shin、Kaneko Takakazu、Gressent Frederic、Nouwen Nico、Arrighi Jean-Francois、Koebnik Ralf、Mergaert Peter、Deslandes Laurent、Giraud Eric
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 43 Pages: 21758-21768

    • DOI

      10.1073/pnas.1904456116

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マメ科植物- 根粒菌共生における新規共生経路の解明2022

    • Author(s)
      岡崎 伸
    • Organizer
      日本農芸化学会2022年度大会【BBB連携シンポジウム】
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] The rhizobial type III effector Bel2-5 plays a dual role in symbiosis with soybean2021

    • Author(s)
      Safirah Tasa Nerves Ratu
    • Organizer
      The 30th Annual Meeting Japanese Society of Plant-Microbe Interaction (Online conference: http://jspmi.brc.miyazaki-u.ac.jp/blog/30th-annual-meeting-2021)
    • Related Report
      2021 Research-status Report
  • [Presentation] Type III effector of Bradyrhizobium elkanii USDA61 Bel2-5 possesses multiple domains determining symbiotic efficiency with soybeans2021

    • Author(s)
      Safirah Tasa Nerves Ratu
    • Organizer
      14th European Nitrogen Fixation Conference (Online conference: https://events.au.dk/enfc2021/conference)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of rhizobial pathogenic-like effector hijacking soybean nodulation signaling2020

    • Author(s)
      Safirah Tasa Nerves Ratu, Christian Oliver Kalaw, Michiko Yasuda, Shin Okazaki
    • Organizer
      International Conferences on Agriculture and Biological Sciences 2020
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Remarks] 東京農工大学 岡崎研究室

    • URL

      http://web.tuat.ac.jp/~okazaki/

    • Related Report
      2021 Research-status Report

URL: 

Published: 2019-07-04   Modified: 2024-01-30  

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