Exploration of novel regulatory mechanisms of translation in bacteria
| Project/Area Number |
19K22715
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Kagoshima University (2020-2022)
Osaka University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
住友 倫子 大阪大学, 大学院歯学研究科, 講師 (50423421)
山口 雅也 大阪大学, 大学院歯学研究科, 准教授 (00714536)
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| Project Period (FY) |
2019-06-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | レンサ球菌 / 翻訳効率 / エンドリボヌクレアーゼ / 化膿レンサ球菌 / 線毛 / mRNA / 細菌 |
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| Outline of Research at the Start |
上気道や皮膚に感染する化膿レンサ球菌などの細菌は，体内コア温度と比較して低温の環境で定着し，感染成立を果たす．一方，病原細菌が感染部位から深部組織に伝播する際，周囲の環境変化に対応して，生存に必要あるいは不必要な因子群を選択する機構が存在すると考えられる．本研究では，温度を感知し，タンパク質への翻訳過程に影響を与える核酸構造を明らかにするとともに，細菌の病原性への関与を検討する．
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| Outline of Final Research Achievements |
The human pathogen Streptococcus pyogenes modulates virulence in response to ambient temperature during the infection process. We searched for bacterial factors that alter translation efficiency in response to temperature shifts. Our findings suggested that not only stem-loop structures located near mRNA translation initiation sites, but also mRNA structures in 5´-untranslated regions are likely involved in the temperature-sensitive translational regulation. In addition, our data revealed that the endoribonuclease RNase Y is crucial for pilus production from strains possessing a specific transcriptional regulator.
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| Academic Significance and Societal Importance of the Research Achievements |
化膿レンサ球菌はヒトに多様な疾患を引き起こす．上市されているワクチンはなく，感染者数の増加が懸念されている．したがって，ワクチン抗原の選択や治療法開発の礎となる病態発症機序の解明が求められる．体内と比較して温度が低い皮膚や咽頭などの初発感染部位から体内へ感染が拡大する際，本菌は環境温度の変化に対応して，様々な因子の発現量を調節すると考えられている．感染過程における細菌の温度感知機構を理解することにより，細菌因子発現部位の予測や治療標的・ワクチン抗原を選択する際の指標となり得る.
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Report
(5 results)
Research Products
(69 results)
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[Journal Article] Pneumococcal BgaA promotes host organ bleeding and coagulation in a mouse sepsis model.?2022
Author(s)
Takemura M, Yamaguchi M, Kobayashi M, Sumitomo T, Hirose Y, Okuzaki D, Ono M, Motooka D, Goto K, Nakata M, Uzawa N, Kawabata S.
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Journal Title
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume: 12
Pages: 844000-844000
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genetic characterization of Streptococcus pyogenes emm89 strains isolated in Japan from 2011 to 2019.2020
Author(s)
Hirose Y, Yamaguchi M, Takemoto N, Miyoshi-Akiyama T, Sumitomo T, Nakata M, Ikebe T, Hanada T, Yamaguchi T, Kawahara R, Okuno R, Otsuka H, Matsumoto Y, Terashima Y, Kazawa Y, Nakanishi N, Uchida K, Akiyama Y, Iwabuchi K. Nakagawa C, Yamamoto K, Nizet V, Kawabata S.
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Journal Title
Infect. Microbe Dis.
Volume: 2
Issue: 4
Pages: 160-166
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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