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Whole-organ analysis of sweat glands in mouse footpad by tissue clearing

Research Project

Project/Area Number 19K23989
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0903:Organ-based internal medicine and related fields
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Tadatsune Iida  東京医科歯科大学, 医学部附属病院, 特任助教 (80844381)

Project Period (FY) 2019-08-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords組織透明化 / 皮膚 / 発汗
Outline of Research at the Start

発汗障害は汗腺の異常か神経系の異常であるかの判断がしばしば難しく、その治療は対症的である。近年、組織透明化が様々な臓器で応用されているが、マウスにおいて唯一汗腺を有する部位である足底の透明化は報告がなく、また皮膚の広範囲3次元画像の自動解析法についても報告がない。本研究は、マウス足底の透明化技術の最適化と自動画像解析システムを作成することにより、発汗障害をきたす疾患における汗腺や神経系の病態解明の基盤を築く。汗腺の形態変化の正確な評価法が確立できれば、患者検体への応用や、病態に沿った治療法の開発に繋がることが期待される。

Outline of Final Research Achievements

The process of sweating involves command from the brain, transmission through peripheral nerves, sweat production and ejection by sweat glands in the skin. However, the cause of the sweating disorders, including primary hyperhidrosis and hypohidrosis, are often not clear. In this study, we established a pipeline for morphological analysis of the whole sweat glands in mouse footpad, for studying mouse models of sweating disorders. The pipeline includes three-dimensional imaging of the whole glands and nerve fibers surrounding them, which could effectively capture morphological changes in the complex structure of the glands. We also developed an image processing program that create a linearized image of sweat gland from the captured image, which could facilitates morphological assessment of the gland.

Academic Significance and Societal Importance of the Research Achievements

多汗症や無汗症などの発汗障害は、その病態が未解明な部分が多く、治療は対症的に行われる場合が多い。発汗は神経系と、汗腺の平滑筋細胞、分泌細胞など多種の細胞の協調によって生じるため、培養細胞を用いた研究には限界があり、マウス等の動物個体を用いた実験が必須である。本研究で確立した、マウスの汗腺を周囲組織を含めて丸ごと形態評価できる新しい手法は、発汗障害の病態解明を通じて医学の発展に寄与するものと考えられる。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (5 results)

All 2021 2020

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Prognosis after steroid pulse therapy and seasonal effect in acquired idiopathic generalized anhidrosis2020

    • Author(s)
      Iida Tadatsune、Nakamura Michiko、Inazawa Minako、Munetsugu Takichi、Nishida Makiko、Fujimoto Tomoko、Sasaki Yoshiyuki、Ohshima Yuichiro、Nakazato Yoshihiko、Namiki Takeshi、Yokozeki Hiroo
    • Journal Title

      The Journal of Dermatology

      Volume: 48 Issue: 3 Pages: 271-278

    • DOI

      10.1111/1346-8138.15666

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 特発性後天性全身性無汗症の病態解析~汗腺の形態変化と炎症細胞浸潤について2021

    • Author(s)
      深江大毅, 飯田忠恒, 宗次太吉, 小林大輔, 横関博雄
    • Organizer
      第120回日本皮膚科学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 特発性後天性全身性無汗症の予後調査結果2020

    • Author(s)
      飯田忠恒,中村美智子,宗次太吉,藤本智子,並木剛,大嶋雄一郎,中里良彦,横関博雄
    • Organizer
      第119回日本皮膚科学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 特発性後天性全身性無汗症の組織学的解析2020

    • Author(s)
      深江大毅, 飯田忠恒, 宗次太吉, 並木剛, 横関博雄
    • Organizer
      第28回日本発汗学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Multicolor three-dimensional reconstruction of whole murine sweat glands using optical clearing and two-photon excitation microscopy2020

    • Author(s)
      Tadatsune Iida, Daiki Fukae, Hiroo Yokozeki
    • Organizer
      The 45th Annual Meeting of The Japanese Society for Investigative Dermatology
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research

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Published: 2019-09-03   Modified: 2022-01-27  

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