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Screening and development of reductive fatty acid- and organic acid-transforming activities in microorganisms

Research Project

Project/Area Number 20380050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

OGAWA Jun  Kyoto University, 大学院・農学研究科, 教授 (70281102)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Sakayu  京都大学, 大学院・農学研究科, 教授 (70093250)
SAKURADANI Eiji  京都大学, 大学院・農学研究科, 助教 (10362427)
KISHINO Shigenobu  京都大学, 大学院・農学研究科, 助教 (40432348)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords還元反応 / 脂肪酸 / 有機酸 / 嫌気反応 / 共役化反応 / 飽和化反応 / 嫌気性微生物 / 嫌気性菌 / 共役脂肪 / Lacctobacillus / Eubacterium / 嫌気性細菌 / Lactobacillus
Research Abstract

Reductive chemical reactions are useful for biomass utilization and biomass transformation to petro-chemical compounds. Reductive metabolisms of fatty acids and organic acids were screened in microorganisms and the reactions, enzymes, and genes involved in the metabolisms were analyzed. Furthermore, the reactions were applied to functional lipid (conjugated fatty acids, hydroxyl fatty acids, and partially saturated fatty acids) production and fatty alcohol production.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (57 results)

All 2011 2010 2009 2008 Other

All Journal Article (26 results) (of which Peer Reviewed: 19 results) Presentation (20 results) Book (7 results) Remarks (4 results)

  • [Journal Article] Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme system requiring oxidoreduction cofactors.2011

    • Author(s)
      Kishino S., J.Ogawa, K.Yokozeki, S.Shimizu
    • Journal Title

      Biosci. Biotechnol. Biochem. 75(2)

      Pages: 318-322

    • NAID

      10027897895

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme system requiring oxidoreduction cofactors2011

    • Author(s)
      S.Kishino, J.Ogawa, et.al.
    • Journal Title

      Biosci.Biotechnol.Biochem

      Volume: 75(2) Pages: 318-322

    • NAID

      10027897895

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] production of conjugated γ-linolenic acid from γ-linolenic acid by Lactobacillus plantarum AKU 1009a.2010

    • Author(s)
      Kishino S., J.Ogawa, A.Ando, K.Yokozeki, S.Shimizu. Microbial
    • Journal Title

      J.Appl.Microbiol. 108(6)

      Pages: 2012-2018

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 特集バイオマスからの有用物質生産プロセス最前線脂肪酸誘導体の合成2010

    • Author(s)
      岸野重信, 小川順
    • Journal Title

      BIO INDUSTRY 27(11)

      Pages: 32-37

    • Related Report
      2010 Final Research Report
  • [Journal Article] Microbial production of conjugated γ-linolenic acid from γ-linolenic acid by Lactobacillus plantarum AKU 1009a2010

    • Author(s)
      S.Kishino, J.Ogawa, et.al.
    • Journal Title

      J.Appl.Microbiol.

      Volume: 108(6) Pages: 2012-2018

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脂肪酸誘導体の合成2010

    • Author(s)
      岸野重信, 小川順
    • Journal Title

      BIO INDUSTRY

      Volume: 27(11) Pages: 32-37

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of conjugated γ-linolenic acid from-γ-linolenic acid by Lactobacillus plantarum AKU 1009a2010

    • Author(s)
      S.Kishino, J.Ogawa, et al.
    • Journal Title

      J.Appl.Microbiol.

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production.2009

    • Author(s)
      岸野重信., 小川順, 横関健三, 清水昌
    • Journal Title

      Appl.Microbiol. Biotechnol. 84(1)

      Pages: 87-97

    • NAID

      120001624125

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of conjugated fatty acids.2009

    • Author(s)
      岸野重信, 小川順, 横関健三, 清水昌
    • Journal Title

      Lipid Technology 21(8-9)

      Pages: 177-181

    • NAID

      120001815261

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shimizu. Selective production of cis-9, trans-11 isomer of conjugated linoleic acidfrom trans-vaccenic acid methyl ester by Delacroixia coronata.2009

    • Author(s)
      Ando, A., J.Ogawa, S.Sugimoto, S.Kishino, E.Sakuradani, K.Yokozeki
    • Journal Title

      J.Appl.Microbiol. 106(5)

      Pages: 1697-1704

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 微生物酵素の産業利用-その最前線を探る-2009

    • Author(s)
      小川順, 清水昌
    • Journal Title

      化学と生物 47(6)

      Pages: 412-418

    • NAID

      10026435732

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 機能性脂質の微生物による生産-アラキドン酸に関連する油脂の発酵生産を中心として-2009

    • Author(s)
      櫻谷英治, 安藤晃規, 小川順, 清水昌
    • Journal Title

      蛋白質核酸酵素 54(6)

      Pages: 725-734

    • NAID

      40016639059

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of conjugated fatty acid2009

    • Author(s)
      S.Kishino, J.Ogawa, et al.
    • Journal Title

      Lipid Technol. 21

      Pages: 177-181

    • NAID

      120001815261

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] metabolic diversity in bionydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production2009

    • Author(s)
      S.Kishino, J.Ogawa, et al.
    • Journal Title

      Appl.Microbiol.Biotechnol. 84

      Pages: 87-97

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective production of cis-9, traps-11 isomer of conjugated linoleic acid from traps-vaccenic acid methyl ester by Delacroixia coronata2009

    • Author(s)
      A.Ando, J.Ogawa, et al.
    • Journal Title

      J.Appl.Microbiol. 106

      Pages: 1697-1704

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 機能性脂質の微生物による生産-アラキドン酸に関連する油脂の発酵生産を中心として-2009

    • Author(s)
      櫻谷英治, 小川順, ら
    • Journal Title

      蛋白質 核酸 酵素 54

      Pages: 725-734

    • NAID

      40016639059

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 微生物酵素の産業利用-その最前線を探る-2009

    • Author(s)
      小川順, 清水昌
    • Journal Title

      化学と生物 47

      Pages: 412-418

    • NAID

      10026435732

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production2009

    • Author(s)
      Kishino, S., J. Ogawa, et al.
    • Journal Title

      Appl. Microbiol. Biotechnol.

    • NAID

      120001624125

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Secretion of flavins by Shewanella species and their role in exracellular electron transfer.2008

    • Author(s)
      von Canstein, H., J.Ogawa, S.Shimizu,J.R.Lloyd.
    • Journal Title

      Appl.Environ. Microbiol. 74(3)

      Pages: 615-623

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] van de Guchtem E. Maguin. Rerouting of pyruvate metabolism during acid adaptation in Lactobacillus bulgaricus.2008

    • Author(s)
      Fernandez, A., J.Ogawa, S.Penaud, S.Boudebbouze, D.Ehrlich, M.
    • Journal Title

      Proteomics 8

      Pages: 3154-3163

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 新春特集微生物機能による反応と物質生産序論-ユニークな微生物機能の探索・開発と物質生産への利用2008

    • Author(s)
      小川順, 岸野重信, 清水昌
    • Journal Title

      月刊ファインケミカル 37(1)

      Pages: 5-7

    • Related Report
      2010 Final Research Report
  • [Journal Article] 乳酸菌による共役脂肪酸生産2008

    • Author(s)
      岸野重信, 小川順, 横関健三, 清水昌
    • Journal Title

      バイオサイエンスとインダストリー 66(2)

      Pages: 54-59

    • NAID

      10024745209

    • Related Report
      2010 Final Research Report
  • [Journal Article] Secretion of flavins by Shewanella species and their role inexracellular electron transfer2008

    • Author(s)
      von Canstein, H., J. Ogawa, et al.
    • Journal Title

      Appl. Environ. Microbiol. 74

      Pages: 615-623

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rerouting of pyruvate metabolism during acid adaptation in Lactobacillus bulgaricus2008

    • Author(s)
      Fernandez, A., J. Ogawa, et al.
    • Journal Title

      Proteomics 8

      Pages: 3154-3163

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 酵素法による共役脂肪酸の合成2008

    • Author(s)
      小川順, ら
    • Journal Title

      ファインケミカル 37

      Pages: 18-27

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 乳酸菌による共役脂肪酸生産2008

    • Author(s)
      岸野重信, 小川順, ら
    • Journal Title

      バイオサイエンスとインダストリー 66

      Pages: 54-59

    • NAID

      10024745209

    • Related Report
      2008 Annual Research Report
  • [Presentation] Linoleic acid isomerase in Lactobacillus plantarum AKU1009a is a multi-componet enzyme system requiring oxidoreduction cofactors.2010

    • Author(s)
      S.Kishino, et. al.
    • Organizer
      101st AOCS Annual Meeting & Expo.
    • Place of Presentation
      Phoenix, USA
    • Year and Date
      2010-05-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Linoleic acid isomerase in Lactobacillus plantarum AKU1009a is a multi-component enzyme system requiring oxidoreduction cofactors2010

    • Author(s)
      S.Kishino, J.Ogawa, et.al.
    • Organizer
      101st AOCS Annual Meeting & Expo
    • Place of Presentation
      Phoenix, USA(招待講演)
    • Year and Date
      2010-05-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 代謝的視点からの機能探索に基づく微生物酵素開発2010

    • Author(s)
      小川順, ら
    • Organizer
      2010年度日本農芸化学会大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] 代謝的視点からの機能探索に基づく微生物酵素開発2010

    • Author(s)
      小川順, ら
    • Organizer
      2010年度日本農芸化学会大会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Metabolic, enzymatic, and genetic studies on biohydrogenation of polyunsaturated fatty acids by anaerobic bacteria involving conjugated fatty acid production.2009

    • Author(s)
      J.Ogawa, et al.
    • Organizer
      5th International Symposiu on Biocatalysis and Biotechnology.
    • Place of Presentation
      Taichung, Taiwan.
    • Year and Date
      2009-11-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Metabolic, enzymatic, and genetic studies on biohydrogenation of polyunsaturated fatty acids by anaerobic bacteria involving conjugated fatty acid production2009

    • Author(s)
      小川順, et al.
    • Organizer
      5th International Symposiu on Biocatalysis and Biotechnology
    • Place of Presentation
      Taichung, Taiwan
    • Year and Date
      2009-11-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] 脂肪酸分子種の多様性を創出する微生物機能2009

    • Author(s)
      小川順, ら
    • Organizer
      第14回水産油脂技術懇話会
    • Place of Presentation
      日本水産油脂協会(東京都)
    • Year and Date
      2009-11-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 脂肪酸分子種の多様性を創出する微生物機能2009

    • Author(s)
      小川順, ら
    • Organizer
      第14回水産油脂技術懇話会
    • Place of Presentation
      日本水産油脂協会 (東京都)
    • Year and Date
      2009-11-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] A multi-component enzyme system for linoleic acid transformation to conjugated linoleic acid (CLA) in lactic acid bacteria.2009

    • Author(s)
      J.Ogawa, et al.
    • Organizer
      15th German-Japanese Workshop on Enzyme Technology.
    • Place of Presentation
      Rostock, Germany.
    • Year and Date
      2009-09-26
    • Related Report
      2010 Final Research Report
  • [Presentation] A multi-component enzyme system for linoleic acid transformation to conjugated linoleic acid (CLA) in lactic acid bacteria2009

    • Author(s)
      小川順, et al.
    • Organizer
      15th German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Rostock, Germany
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] A multi-component enzyme system for linoleic acid transformation to conjugated linoleic acid (CLA) in lactic acid bacteria.2009

    • Author(s)
      J.Ogawa, et al.
    • Organizer
      Enzyme Engineering XX.
    • Place of Presentation
      Groningen, Holland.
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] A multi-component enzyme system for linoleic acid transformation to conjugated linoleic acid (CLA) in lactic acid bacteria2009

    • Author(s)
      小川順, et al.
    • Organizer
      Enzyme Engineering XX
    • Place of Presentation
      Groningen, Holland
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Protentials of Microorganisms for Functional Food Production and Probiotics.2009

    • Author(s)
      小川順, ら, et al.
    • Organizer
      Workshop Argentina-Japan "Bioscience and Biotechnology for the Promotion of Agriculture and Food Production".
    • Place of Presentation
      Buenos Aires, Argentina.
    • Year and Date
      2009-08-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Protentials of Microorganisms for Functional Food Production and Probiotics2009

    • Author(s)
      小川順, et al.
    • Organizer
      Workshop Argentina-Japan "Bioscience and Biotechnology for the Promotion of Agriculture and Food Production"
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      2009-08-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 微生物機能を活用した様々な機能性脂質素材の生産2009

    • Author(s)
      小川順, ら
    • Organizer
      2009年油脂産業技術部会セミナー-有用油脂の高度利用「油脂と健康」-
    • Place of Presentation
      油脂工業会館(東京都)
    • Year and Date
      2009-05-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 微生物機能を活用した様々な機能性脂質素材の生産2009

    • Author(s)
      小川順, ら
    • Organizer
      2009年油脂産業技術部会セミナー-有用油脂の高度利用「油脂と健康」-
    • Place of Presentation
      油脂工業会館 (東京都)
    • Year and Date
      2009-05-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Potentials of lactic acid bacteria for functional lipid production and anti-hyperuricemia probiotics.2008

    • Author(s)
      小川順, ら, et al.
    • Organizer
      The 11th Swiss-Japanese Joint Meeting on Biotechnology and Bioprocess Development.
    • Place of Presentation
      Minusio, Switzerland.
    • Year and Date
      2008-10-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Potentials of lactic acid bacteria for functional lipid production and anti-hyperuricemia probiotics2008

    • Author(s)
      Ogawa, J., et al.
    • Organizer
      The 11th Swiss-Japanese Joint Meeting on Biotechnology and Bioprocess Development
    • Place of Presentation
      Minusio, Switzerland
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Unique fatty acid transformation catalyzed by anaerobic bacteria and its application for conjugated fatty acid production.2008

    • Author(s)
      小川順, ら, et al.
    • Organizer
      The 2008 Annual Meeting of The Society for Industrial Microbiology.
    • Place of Presentation
      San Diego, USA.
    • Year and Date
      2008-08-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Uniaue fatty acid transforaation catalyzed by anaerobic bacteria and its application for conjugated fatty acid production2008

    • Author(s)
      Ogawa, J., et al.
    • Organizer
      The 2008 Annual Meeting of The Society for Industrial Microbiology
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2008-08-11
    • Related Report
      2008 Annual Research Report
  • [Book] John Wiley & Sons, 62010

    • Author(s)
      櫻谷英治, 小川順, 岸野重信, 安藤晃規, 横関健三, 清水昌
    • Publisher
      Oils, Microbial Production. Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology (ed.by M.C. Flickinger)
    • Related Report
      2010 Final Research Report
  • [Book] 第5編-第1章-第5節「3.有用脂肪酸の生産」.酵素利用技術大系(小宮山眞監修)2010

    • Author(s)
      小川順, 櫻谷英治, 岸野重信, 安藤晃規, 清水昌
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2010 Final Research Report
  • [Book] 第7章バイオプロダクトと新プラットフォーム形成「9.脂肪酸誘導体の合成」.地球環境シリーズ エコバイオリファイナリー-脱石油社会へ移行するための環境ものづくり戦略-、(植田充美、田丸浩監修)2010

    • Author(s)
      岸野重信, 小川順
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2010 Final Research Report
  • [Book] 高度不飽和脂肪酸・共役脂肪酸含有油脂の微生物生産.バイオテクノロジーシリーズ微生物によるものづくり-化学法に代わるホワイトバイオテクノロジーのすべて-,植田充美監修, 東京2010

    • Author(s)
      小川順, 岸野重信, 櫻谷英治, 清水昌
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Final Research Report
  • [Book] 酵素利用技術大系(小宮山眞監修)(エヌ・ティー・エス出版)2010

    • Author(s)
      小川順, ら
    • Total Pages
      4
    • Publisher
      有用脂肪酸の生産
    • Related Report
      2010 Annual Research Report
  • [Book] 微生物機能を活用した食品機能の創出.栄養学研究の最前線,日本栄養食糧学会監修2008

    • Author(s)
      小川順, 岸野重信, 櫻谷英治, 横関健三, 清水昌
    • Publisher
      建帛社
    • Related Report
      2010 Final Research Report
  • [Book] 微生物機能を活用した食品機能の創出/栄養学研究の最前線(日本栄養食糧学会監修)2008

    • Author(s)
      小川順, ら
    • Publisher
      建帛社
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.hakko.kais.kyoto-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.hakko.kais.kyoto-u.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.microbial.kais.kyoto-u.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.sangyo.kais.kyoto-u.ac.jp/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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