Development of innovative rumen modifier to reduce methane production

• Project/Area Number: 20380146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Hokkaido University
• Principal Investigator: KOBAYASHI Yasuo Hokkaido University, 大学院・農学研究院, 教授 (50153648)
• Co-Investigator(Kenkyū-buntansha): KOIKE Satoshi 北海道大学, 大学院・農学研究院, 助教 (90431353) ; ENISHI Osamu 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所, チーム長 (20355069) ; TAKENAKA Akio 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所, チーム長 (40155031)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: ルーメン / メタン / 発酵 / 微生物 / 栄養 / プロピオン酸 / カシューナッツ殻油 / 選択的抗菌性 / 真正細菌 / 古細菌 / 菌叢 / カシュレナッツ殻油 / 安全性 / 抗菌スペクトラム / 抗菌性物質

Research Abstract

Cashew nut shell liquid, a novel rumen modifier, was evaluated for its potential to reduce methane from ruminant animals and possible mechanisms involved in it. The shell liquid selectively inhibited growth of several species of rumen bacteria to lead a dramatic shift of rumen microbiota. This resulted in rumen fermentation changes with less methane and more propionate production but without adverse effects on feed digestion and animal health. The feeding of shell liquid allowed more than 20% reduction of methane that had not been realized by any other modifiers. Therefore, future application of the shell liquid is highly expected.

Research Products

• [Journal Article] 家畜からのメタン生成を低減する天然物質の探索(ミニレビュー) (2011)
  • Author(s): 小林泰男
  • Journal Title: 日本農薬学会誌 36 Pages: 124-126
  • Peer Reviewed
• [Journal Article] In vitro evaluation of cashew nut shell liquid as a methane-inhibiting and propionate-enhancing agent for ruminants. (2011)
  • Author(s): Watanabe, Y., R.Suzuki, S.Koike, K.Nagashima, M.Mochizuki, R.J.Forster, Y.Kobayaehi.
  • Journal Title: Journal of Dairy Science Volume: 93 Pages: 5258-5267
  • Peer Reviewed
• [Journal Article] In vitro evaluation of cashew nut shell liquid as a methane-inhibiting and propionate-enhancing agent for ruminants. (2010)
  • Author(s): Watanabe Y., R.Suzuki, S.Koike, K.Nagashima, M.Mochizuki, R.J.Foster, Y.Kobayashi
  • Journal Title: Journal of Dairy Science. 93 Pages: 5258-5267
  • Peer Reviewed
• [Journal Article] Abatement of methane production from ruminants : trends in the manipulation of rumen fermentation(総説) (2010)
  • Author(s): 小林泰男
  • Journal Title: Asian-Australasian Journal of Animal Sciences 23 Pages: 410-416
  • Peer Reviewed
• [Journal Article] Detection and identification of rumenbacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes (2010)
  • Author(s): Shinkai, T., T.Ueki, Y.Kobayashi.
  • Journal Title: Animal Science Journal Volume: 81 Pages: 72-79
  • NAID: 10027888601
  • Peer Reviewed
• [Journal Article] Abatement of methane production from ruminants : trends in the manipulation of rumen fermentation. (2010)
  • Author(s): Kobayashi, Y.
  • Journal Title: Asian・Australasian Journal of Animal Sciences Volume: 23 Pages: 410-416
  • Peer Reviewed
• [Journal Article] Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes. (2010)
  • Author(s): Shinkai, T., T.Ueki, Y.Kobayashi
  • Journal Title: Animal Science Journal 81 Pages: 72-79
  • NAID: 10027888601
  • Peer Reviewed
• [Journal Article] Effective degradation of tannic acid by immobilized rumen microbes of a sika deer(Cervus Nippon yesoensis)in winter (2010)
  • Author(s): Hiura, T., Y.Hashidoko, Y.Kobayashi, S.Tahara
  • Journal Title: Animal Feed Science and Technology 155 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Fibrolytic capabilities of ruminal bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping (2009)
  • Author(s): Shinkai, T., R.Ohji, N.Matsumoto, Y.Kobayashi
  • Journal Title: FEMS Microbiology Letters 294 Pages: 183-190
  • Peer Reviewed
• [Journal Article] Fibrolytic rumen bacteria : their ecology and functions (2009)
  • Author(s): Koike, S. and Y. Kobayashi
  • Journal Title: Asian-Australasian Journal of Animal Sciences 22 Pages: 131-138
  • Peer Reviewed
• [Journal Article] Ecological and physiological characterization shows that Fibrobacter succinogenes is important in rumen fiber digestion (2008)
  • Author(s): Kobayashi, Y., T. Shinkai and S. Koike
  • Journal Title: Folia Microbiologica 53 Pages: 195-200
  • Peer Reviewed
• [Journal Article] Cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain of Eubacterium cellulosolvens (2008)
  • Author(s): Taguchi, H., T. Senoura, 他5名
  • Journal Title: FEMS Microbiology Letters 287 Pages: 34-40
  • Peer Reviewed
• [Presentation] Methane reduction from ruminants by using cashew nut shell liquid containing rare phenolic compounds. (2011)
  • Author(s): 小林泰男
  • Organizer: GRA -Rumen Microbial Genomics Workshop
  • Place of Presentation: Palmerston North, New Zealand
• [Presentation] 新規メタン低減剤カシューナッツ殻液によるヒツジルーメン内細菌叢の変動 (2010)
  • Author(s): 林秀輔・渡部優・鈴木亮・小池聡・長嶋協・望月正己・小林泰男
  • Organizer: 第65回北海道畜産学会(講演要旨p21)
  • Place of Presentation: 道立総合研究機構畜産試験場(新得)
  • Year and Date: 2010-09-09
• [Presentation] Methane reduction and associated microbial changes in the rumen (2010)
  • Author(s): Kobayashi, Y.
  • Organizer: 2^<nd> International Symposium on Gastrointestinal Microbial Ecology and Functionality
  • Place of Presentation: Nanjing Agricultural University, Nanjing, China(招待講演)
  • Year and Date: 2010-05-23
• [Presentation] カシューナッツ殻液給与がヒツジルーメンの真性細菌およびメタン細菌叢におよぼす影響 (2010)
  • Author(s): 渡部優・鈴木亮・小池聡・長嶋協・望月正巳・小林泰男
  • Organizer: 日本畜産学会第112回大会(講演要旨P12)
  • Place of Presentation: 明治大学
  • Year and Date: 2010-03-28
• [Presentation] Cashew nut shell liquid, a novel candidate feed additives for ruminants : potency as a methane-reducing agent. (2010)
  • Author(s): Kobayashi Y., R.Suzuki, Y.Watanabe, S.Koike, M.Mochizuki, K.Nagashima
  • Organizer: Greenhouse Gases and Animal Agriculture Conference
  • Place of Presentation: Banff, Canada
• [Presentation] Bacterial and archaeal community shifts by feeding of the novel methane-reducing agent cashew nut shell liquid. (2010)
  • Author(s): Hayashi S., Y.Watanabe, R.Suzuki, S.Koike, K.Nagashima, M.Mochizuki, Y.Kobayashi
  • Organizer: Greenhouse Gases and Animal Agriculture Conference
  • Place of Presentation: Banff, Canada
• [Presentation] Effect of cashew nut shell liquid on bacterial and archaeal community in the rumen of cattle. (2010)
  • Author(s): Su C., T.Shinkai, M.Mitsumori, O.Enishi, K.Nagashima, M.Mochizuki, S.Koike, Y.Kobayashi
  • Organizer: Greenhouse Gases and Animal Agriculture Conference
  • Place of Presentation: Banff, Canada
• [Presentation] Methane reduction and associated microbial changes in the rumen. (2010)
  • Author(s): 小林泰男
  • Organizer: 2nd International Symposium on Gastrointestinal Microbiology.
  • Place of Presentation: Nanjing, China
• [Presentation] カシューナッツ殻液給与がウシルーメン菌叢に及ぼす影響. (2010)
  • Author(s): 嵩千里・真貝拓三・三森真琴・永西修・長嶋協・望月正己・小池聡・小林泰男
  • Organizer: 日本畜産学会第112回大会(講演要旨P12)
  • Place of Presentation: 明治大学
• [Presentation] Abatement of methane production from ruminants : trends in the manipulation of rumen fermentation. (2009)
  • Author(s): 小林泰男
  • Organizer: International Symposium on Improvement of Feed Efficiency through Biotechnology
  • Place of Presentation: Beijing, China.
  • Year and Date: 2009-11-16
• [Presentation] Anaerobic rumen microbes involved in methane reduction by dietary manipulations. (2009)
  • Author(s): 小林泰男
  • Organizer: The 6th International Symposium on Anaerobic Microbiology
  • Year and Date: 2009-06-19
• [Presentation] Anaerobic rumen microbes involved in methane reduction by dietary manipulations (2009)
  • Author(s): Kobayashi, Y.
  • Organizer: 6^<th> International Symposium of Anaerobic Microbiology
  • Place of Presentation: Prague, Czech
  • Year and Date: 2009-06-19
• [Presentation] 新規天然抗菌物質が各種ルーメン細菌の増殖に及ぼす影響 (2009)
  • Author(s): 林秀輔・鈴木亮・小池聡・小林泰男・長嶋協・望月正巳・伊藤真治・金田晃一
  • Organizer: 北海道畜産学会第64回大会(講演要旨P24)
  • Place of Presentation: 道立根釧農業試験場
• [Presentation] 新規天然抗菌物質によるルーメン発酵制御作用の評価 (2009)
  • Author(s): 嵩千里・鈴木康之・小池聡・小林泰男・長嶋協・望月正巳・伊藤真治・金田晃一
  • Organizer: 北海道畜産学会第64回大会(講演要旨P24)
  • Place of Presentation: 道立根釧農業試験場
• [Presentation] Potency of cashew nut shell liquid as a methan-reducing and propionate-enhancing agent for ruminants. (2009)
  • Author(s): Watanabe Y., R.Suzuki, 小池聡, K.Nagashima, M.Mochizuki, Y.Kobayashi
  • Organizer: The 7th Korea-Japan-China Joint Symposium on Rumen Metabolism and Physiology
  • Place of Presentation: Seoul, Korea
• [Presentation] Exploration of, plant-associated rumen bacteria : their phylogeny, ecology and functions (2008)
  • Author(s): Kobayashi, Y. and S. Koike
  • Organizer: 12th International Society for Microbial E cologv Conference
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-08-18
• [Book] 気象ブックス(畜産と気象),(柴田正貴・寺田文典編)(第3章「家畜の消化機能」第6章「家畜と温暖化」) (2010)
  • Author(s): 小林泰男
  • Publisher: 成山堂,東京
• [Book] 気象ブックス(畜産と気象)(第3章「家畜の消化機能」および第6章「家畜と温暖化」)(柴田正貴・寺田文典編) (2010)
  • Author(s): 小林泰男
  • Total Pages: 173
  • Publisher: 成山堂
• [Book] 国産飼料の利用拡大に対応した乳牛の栄養管理 (2009)
  • Author(s): 小林泰男
  • Total Pages: 193
  • Publisher: デーリィマン社
• [Book] 微生物増殖学の現在・未来 (2008)
  • Author(s): 小林泰男
  • Total Pages: 478
  • Publisher: 地人書館
• [Remarks] 報道関係 世の中面白研究所.NHKラジオ第一,2008年7月7日. Touch Eco 2008,NTV.2008年6月20日 エコアイデアツアーメタンガス編,National.山手線トレインチャンネル,2008年6月2日. ネイチャー特集,読売新聞.2008年5月19日 What's up Japan?.NHK国際ラジオ.2008年5月5日. ゲップ減らして地球を守れ.ゆうどきネットワーク(NHK東京),2008年4月4日. 牛ゲップ対策で新発明.ワイド!スクランブル,TV朝日,2008年4月3日. メタンガス削減新物質発見.まるごとニュース北海道(NHK札幌),2008年3月27日. 牛のゲップ減らす物質発見.(NHKニュース、朝ズバ、特ダネ、スッキリ他),2008年3月25日. 牛の餌にカシューナッツの油でメタン9割抑制.北海道新聞(他7紙),2008年3月25日. ホームページ等
  • URL: http://www.agr.hokudai.ac.jp/anim/nutr/
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  • URL: http://www.agr.hokudai.ac.jp/anim/nutr/
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  • URL: http://www.agr.hokudai.ac.jp/animinutr/geppu.htm