Elucidation of bacterial pathogenesis system based on a silkworm infection model
Project/Area Number |
20390021
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | The University of Tokyo |
Principal Investigator |
SEKIMIZU Kazuhisa The University of Tokyo, 大学院・薬学系研究科, 教授 (90126095)
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Co-Investigator(Kenkyū-buntansha) |
KAITO Chikara 東京大学, 大学院・薬学系研究科, 准教授 (60420238)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
Keywords | カイコ / 病原性 / 病原性因子 / コロニースプレッデング / 病院分離型MRSA / 市中感染型MRSA / psm-mec遺伝子 / 可動遺伝要素 / SCCmec / バイオフィルム / 黄色ブドウ球菌 / 自然免疫 / 脂質輸送 |
Research Abstract |
In this study, we performed to solve molecular function of CvfB (conserved virulence factor B), which was identified as a virulence regulatory factor using silkworm infection model. CvfB has three S1 RNA binding domain and a Winged-Helix domain revealed by crystal structure at 1.4Å resolution. Moreover, we uncovered the RNA binding activity of CvfB is required for hemolysin production in Staphylococcus aureus.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.2011
Author(s)
Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiramatsu K, Sekimizu K.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Transcription and Translation Products of the Cytolysin Gene psm-mec on the Mobile Genetic Element SCCmec Regulate Staphylococcus a ureus Virulence.2011
Author(s)
Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiram atsu K, Sekimizu K.
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Journal Title
PLoS Pathog.
Volume: 3;7(2)
Related Report
Peer Reviewed
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[Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module.2010
Author(s)
Matsumoto Y, Xu Q, Miyazaki S, Kaito C, Farr CL, Axelrod HL, Chiu HJ, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Sekimizu K, Wilson IA.
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Journal Title
Structure. 18(4)
Pages: 537-547
Related Report
Peer Reviewed
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[Journal Article] Cell-permeable carboxy-terminal p27Kipl peptide exhibits anti-tumor activity by inhibiting Pim-1 kinase2010
Author(s)
Morishita D, Takami M, Yoshikawa S, Katayama Ryohei, Sato S, Kukimoto-Niino M, Umeh ara T, Shirouzu M, Sekimizu K, Yokoyama S, Fujita N
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Journal Title
J.Biol.Chem.
Volume: 286
Pages: 2681-2688
Related Report
Peer Reviewed
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[Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winge d-helix RNA binding module2010
Author(s)
Matsumoto Y, Xu Q, Kaito C, Fan C L, Axelrod H L, Chiu H-J, Klock H E, Knuth M W, Miller M D, Elsliger M-A, Deacon A M, Godzik A, Lesley S A, Sekimizu K, Wilson I A
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Journal Title
Structure
Volume: 18(4)
Pages: 537-547
Related Report
Peer Reviewed
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[Journal Article] Structure of a virulence regulatory factor CvfB reveals a novel winged-helix RNA binding module2010
Author(s)
Matsumoto Y, Xu Q, Kaito C, Farr C L, Axelrod H L, Chiu H-J, Klock H E, Knuth M W, Miller M D, Elsliger M-A, Deacon A M, Godzik A, Lesly S A, Sekimizu K, Wilsom I A
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[Journal Article] Prevention of death in bacterium-infected mice by a synthetic antimicrobial peptide, L5, through activation of host immunity2009
Author(s)
Okuyama-Nishida Y, Akiyama N, Sugimori G, Nomura K, Ogawa K, Homma K J, Sekimizu K, Tsujimoto M, Natori S
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Journal Title
Antimicrob Agents Chemother 53
Pages: 2510-2516
Related Report
Peer Reviewed
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[Journal Article] Auxiliary role for d-alanylated wall teichoic acid in Toll-like receptor 2-mediated survival of Staphylococcus aureus in macrophages2009
Author(s)
Shiratsuchi A, Shimizu K, Watanabe I, Hashimoto Y, Kurokawa K, Razanajatovo I M, Park K H, Park H K, Lee B L, Sekimizu K, Nakanishi Y
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus.2008
Author(s)
Kaito C, Omae Y, Matsumoto Y, Nagata M, Yamaguchi H, Aoto T, Ito T, Hiramatsu K, Sekimizu K.
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