Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

• Project/Area Number: 20570034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: SAZUKA Takashi Nagoya University, 生物機能開発利用研究センター, 准教授 (70362291)
• Co-Investigator(Renkei-kenkyūsha): KITANO Hidemi 名古屋大学, 生物機能開発利用研究センター, 教授 (50144184)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: イネ / 胚発生 / 器官形成 / オーキシン / マイクロRNA / 植物ホルモン / 生長生理 / 国際情報交換

Research Abstract

The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.

Research Products

• [Journal Article] Mapping and characterization of the rice gene (2010)
  • Author(s): Imamura, K., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
  • Journal Title: CLUB-SHAPED EMBRYO3, CLE3.Rice Gen.Newslett. 25 Pages: 27-29
• [Journal Article] Characterization and mapping of the CROWN ROOTLESS2 gene (2010)
  • Author(s): Yamamoto, Y., Inukai, Y., Kitano, H., Sazuka, T., Matsuoka, M.
  • Journal Title: CRL2, in rice.Rice Gen.Newslett. 25 Pages: 25-26
• [Journal Article] Characterization and mapping of the CROWN ROOTLESS2 gene, CRL2, in rice. (2010)
  • Author(s): Yamamoto, Y., et al.
  • Journal Title: Rice Gen.Newslet. Volume: 25 Pages: 25-26
  • Peer Reviewed
• [Journal Article] Mappring and characterization of the rice gene, CLUB-SHAPED EMBRY03, CLE3. (2010)
  • Author(s): Imamura, K., et al.
  • Journal Title: Rice Gen.Newslet. Volume: 25 Pages: 27-29
  • Peer Reviewed
• [Journal Article] A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. (2009)
  • Author(s): Sazuka, T., Kamiya, N., Nishimura, T., Ohmae, K., Sato, Y., Imamura, K., Nagato, Y., Koshiba, T., Nagamura, Y., Ashikari, M., Kitano, H., Matsuoka, M.
  • Journal Title: Plant J. 60 Pages: 227-241
• [Journal Article] A rice trptophan deficient dwarfmutant, tddl, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. (2009)
  • Author(s): Sazuka T., et al.
  • Journal Title: Plant J. 60 Pages: 227-241
  • Peer Reviewed
• [Journal Article] Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1. (2008)
  • Author(s): Imamura, K., Imai, H., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
  • Journal Title: Rice Gen.Newslett. 4 Pages: 22-24
• [Journal Article] Mapping and Analysis of the CLUB-SHAPED EMBRYOl, CLEl. (2008)
  • Author(s): Imamura, K., Imai, H., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
  • Journal Title: Rice Gen. Newslet. 24 Pages: 22-24
  • Peer Reviewed
• [Remarks] ホームページ等
  • URL: http://www.agr.nagoya-u.ac.jp/~naikan/