The development of a ultra-sensitive diagnostic technique for BSE(Mad cow disease)

• Project/Area Number: 20580354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: The University of Tokushima
• Principal Investigator: YAMAGUCHI Yoshitaka The University of Tokushima, 疾患酵素学研究センター, 助教 (90432765)
• Co-Investigator(Kenkyū-buntansha): SAKAGUCHI Suehiro 徳島大学, 疾患酵素学研究センター, 教授 (60274635)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 獣医学 / 蛋白質 / 脳神経疾患

Research Abstract

This research was designed to develop a ultra-sensitive detection method for prion disease. We tried to establish the detection method with high sensitivity and singularity, according to amplify the DNA which uniquely bound to the prion protein and decide the sequence. Though the DNA sequence GGATG was identified from REPSA method as a candidate of consensus sequence, it didn't support sufficient for reproducibility and specificity in the latest study.

Research Products

• [Journal Article] Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions (2011)
  • Author(s): Koji Fujita
  • Journal Title: Cellular and Molecular Neurobiology in press
  • Peer Reviewed
• [Journal Article] Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. (2010)
  • Author(s): Koji Fujita, Yoshitaka Yamaguchi, suyoshi Mori, Naomi Muramatsu, Takahito Miyamoto, Masashi Yano, Hironori Miyata, Akira Ootsuyama, Makoto Sawada, Haruo Matsuda, Ryuji Kaji , Suehiro Sakaguchi.
  • Journal Title: Cellular and Molecular Neurobiology in press
  • Peer Reviewed
• [Journal Article] Dominant-negative effects of the N-terminal half of prion protein on neurotoxicity of prion protein-like rotein/doppel in mice. (2008)
  • Author(s): Yoshikawa D, Yamaguchi N, Ishibashi D, Yamanaka H, Okimura N, Yamaguchi Y, Mori T, Miyata H, Shigematsu K, Katamine S, Sakaguchi S.
  • Journal Title: J Biol Chem. 283(35) Pages: 24202-11
  • Peer Reviewed
• [Journal Article] Cellular prion protein prevents brain damage after encephalomyocarditis virus infection in mice. (2008)
  • Author(s): Nasu-Nishimura Y, Taniuchi Y, Nishimura T, Sakudo A, Nakajima K, Ano Y, Sugiura K, Sakaguchi S, Itohara S, Onodera T.
  • Journal Title: Arch Virol. 153(6) Pages: 1007-12
  • Peer Reviewed
• [Presentation] プリオン蛋白構造変換におけるアミノ酸91-104領域の役割 (2010)
  • Author(s): 山口仁孝、村松直美、森剛志、坂口末廣
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] 異なるプリオン株に異なる感受性を示すプリオン蛋白:プリオン株産生メカニズムについての考察 (2010)
  • Author(s): 坂口末廣、宮田博規、山口仁孝、村松直美、森剛志
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] プリオン蛋白構造変換におけるアミノ酸911104領域の役割 (2010)
  • Author(s): 山口仁孝
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] プリオン構造変換に関与するN末端領域の機能解析 (2009)
  • Author(s): 山口仁孝、村松直美、藤田浩司、坂口末廣
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] プリオン蛋白オクタペプチドリピート領域はプリオンの病原性を決定する (2009)
  • Author(s): 宮田博規、山口仁孝、坂口末廣
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25