A New treatment strategy using analysis of anti-transcriptional factors antibody in connective tissue diseases.

• Project/Area Number: 20591179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: SAITO Kazuyoshi University of Occupational and Environmental Health, Japan, 医学部, 准教授 (30279327)
• Co-Investigator(Kenkyū-buntansha): KOHNO Kimitoshi 産業医科大学, 医学部, 教授 (00153479)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Yoshiya 産業医科大学, 医学部, 教授 (30248562)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膠原病学 / 抗転写因子抗体 / 膠原病 / 転写因子 / 自己抗体

Research Abstract

We developed a new detection system for anti-body against transcriptional factors in connective tissue diseases. Ninety six nuclear factors that involved in cell cycle and homeostasis were blotted on the DEAE paper and incubated with serum from the patients with connective tissue diseases including SLE, dermatomyositis, polyangitis. Anti-HMG1 antibody was detected in 2 patients with polyangitis. Antibody against PCNA was observed in 2 SLE but the serum reacted with a lot of nuclear factors irrelevant with organ specificity. Acetylation and methylation of nuclear factors did not affect on the result of detection of auto-antibody.

Research Products

• [Journal Article] Phenotypic Changes of Lymphocytes in Patients with Systemic Lupus Erythematosus who are in the long-term remission After B Cell Depletion Therapy with Rituximab (2011)
  • Author(s): Iwata S, Saito K, Tokunaga M, Yamaoka K, Nawata M, Yukawa S, Hanami K, Fukuyo S, Kubo S, Miyagawa I, Tanaka Y
  • Journal Title: Journal of Rheumatology 38(4) Pages: 633-41
  • Peer Reviewed
• [Journal Article] Efficacy of combination therapy of anti-TNF-α antibody infliximab and methotrexate in refractory entero-Behcet's disease. (2011)
  • Author(s): Iwata S, Saito K, Yamaoka K, Tsujimura S, Nawata M, Hanami K, Tanaka Y
  • Journal Title: Mod Rheumatol. 21(2) Pages: 184-91
  • NAID: 10029361883
  • Peer Reviewed
• [Journal Article] Phenotypic Changes of Lymphocytes in Patients with Systemic Lupus Erythematosus who are in the long-term remission After B Cell Depletion Therapy with Rituximab (2011)
  • Author(s): Iwata S, Saito K, et al.
  • Journal Title: Journal of Rheumatology Volume: in press
  • Peer Reviewed
• [Journal Article] Efficacy of combination therapy of anti-TNF-a antibody infliximab and methotrexate in refractory entero-Behcet's disease (2011)
  • Author(s): Iwata S, Saito K, et al.
  • Journal Title: Mod Rheumatol Volume: in press
  • Peer Reviewed
• [Journal Article] Etanercept overcomes P-glycoprotein-induced drug resistance in lymphocytes of patients with intractable rheumatoid arthritis. (2010)
  • Author(s): Tsujimura S, Saito K, Nakayamada S, Tanaka Y
  • Journal Title: Modern Rheumatology 20(2) Pages: 139-146
  • NAID: 10027168842
  • Peer Reviewed
• [Journal Article] Tacrolimus, a calcineurin inhibitor, overcomes treatment unresponsiveness mediated by P-glycoprotein on lymphocytes in refractory rheumatoid arthritis. (2010)
  • Author(s): Suzuki K, Saito K, Tsujimura S, Nakayamada S, Yamaoka K, Sawamukai N, Iwata S, Nawata M, Nakano K, Tanaka Y
  • Journal Title: J Rheumatol. 37(3) Pages: 512-520
  • Peer Reviewed
• [Journal Article] Y-box binding protein-1 is a novel molecular target for tumor vessels. (2010)
  • Author(s): Takahashi M, Shimajiri S, Izumi H, Hirano G, Kashiwagi E, Yasuniwa Y, Ying W, Han B, Akiyama M, Nishizawa S, Sasaguri Y, Kohno K
  • Journal Title: Cancer Sci. 101 Pages: 1367-1373
  • Peer Reviewed
• [Journal Article] Etanercept overcomes P-glycoprotein-induced drug resistance in lymphocytes of patients with intractable rheumatoid arthritis (2010)
  • Author(s): Tsujimura S, Saito K,, et al.
  • Journal Title: Modern Rheumatology (in press)
  • NAID: 10027168842
  • Peer Reviewed
• [Journal Article] Tacrolimus, a calcineurin inhibitor, overcomes treatment unresponsiveness me diated by P-glycoprotein on lymphocytes in refractory rheumatoid arthritis (2010)
  • Author(s): Suzuki K, Saito K, et al.
  • Journal Title: J Rheumatol. 37(3) Pages: 512-520
  • Peer Reviewed
• [Journal Article] Effects of anti-TNF-alpha antibody infliximab in refractory entero-Behcet's disease. (2009)
  • Author(s): Iwata S, Saito K, Yamaoka K, Tsujimura S, Nawata M, Suzuki K, Tanaka Y
  • Journal Title: Rheumatology 48(8) Pages: 1012-1013
  • Peer Reviewed
• [Journal Article] Effects of anti-TNF-alpha antibody infliximab in refractory entero-Behcet's disease. (2009)
  • Author(s): Iwata S, Saito K, et al.
  • Journal Title: Rheumatology 48(8) Pages: 1012-1013
  • Peer Reviewed
• [Presentation] Humanized anti human Interlukin-6 receptor monoclonal antibody tocilizumab (TCZ) increases high molecular weight adiponectin (HMW-AN) in the patients with rheumatoid arthritis (RA) : result from The ESCORT study (2010)
  • Author(s): Saito K, Hanami k, Nawata M, Yunoue N, Tanaka Y
  • Organizer: The 11th Annual European Congress of Rheumatology (EULAR)
  • Place of Presentation: Roma(Fiera Rome)
  • Year and Date: 2010-06-16
• [Presentation] Humanized anti-human Intedukin-6 receptor monoclonal antibody tocilizumab(TCZ)increases high molecules weight adiponectin(HMW-AN)in the patients with rheumatoid arthritis(RA): result from The ESCORT study (2010)
  • Author(s): Saito K
  • Organizer: The 11th Annual European Congress of Rheu matology(EULAR)
  • Place of Presentation: Roma(Fiera Rome)
  • Year and Date: 2010-06-16