Bladder reconstruction by autologous bone-marrow-derived mesenchymal stem cell

• Project/Area Number: 20591877
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: ISHIZUKA Osamu Shinshu University, 医学部, 准教授 (20184541)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Tetsuya 信州大学, 医学部, 研究員 (00467143)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨髄幹細胞 / 再生医療 / 膀胱 / 移植・再生医療 / 再生医学 / 細胞・組織 / 生体機能利用 / 脳神経疾患

Research Abstract

From the clinical point of view, urethral sphincter reconstruction is important to prevent urinary incontinence in the field of bladder reconstruction. At first, we could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit. The cultured stem cells were implanted into the injured urethral sphincter, and we could found the reconstruction of the urethral sphincter by histological methods (immunohistochemistry, RT-PCR method) and functional study.

Research Products

• [Journal Article] Implantation of autologous bone marrow-derived cells reconstructs functional urethral sphincters in rabbits (2010)
  • Author(s): Imamura, T., Ishizuka, O., Kinebuchi, Y., Kurizaki, Y., Nakayama, T., Ishikawa, M., Nishizawa, O.
  • Journal Title: Tissue Eng.(Epub ahead of print)
  • NAID: 120007110472
  • Peer Reviewed
• [Journal Article] Rat bladders augmented with a novel bovine pericardium-derived biomaterial reconstruct functional tissue structures. (2010)
  • Author(s): Mimura, Y., Imamura, T., Kinebuchi, Y., Aizawa, N., Ishizuka, O., Nishizawa, O.
  • Journal Title: LUTS 2 Pages: 76-82
  • Peer Reviewed
• [Journal Article] Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. (2010)
  • Author(s): Kinebuchi, Y., Aizawa, N., Imamura, L Ishizuka, O., Igawa, Y., Nishizawa, O.
  • Journal Title: Int.J.Urol. 17 Pages: 359-368
  • NAID: 10027258036
  • Peer Reviewed
• [Journal Article] Differentiation of smooth muscle cells from human amniotic mesechymal cells implanted in the freeze-injured mouse urinary bladder. (2010)
  • Author(s): Minagawa, T., Imamura, T.,Ishizuka, O., Igawa, Y., Nishizawa, O.
  • Journal Title: Eur.Urol. Volume: 58 Pages: 299-306
  • Peer Reviewed
• [Journal Article] Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. (2010)
  • Author(s): Kinebuchi, Y., Aizawa, N., Imamura, T., Ishizuka, 0., Igawa, Y., Nishizawa, 0.
  • Journal Title: Int. J.Urol. Volume: 17 Pages: 359-368
  • NAID: 10027258036
  • Peer Reviewed
• [Journal Article] Rat bladders augmented with a novel bovine pericardium-derived-biomate rial reconstruct functional tissue structures. (2010)
  • Author(s): Mimura, Y., Imamura, T., Kinebuchi, Y., Aizawa, N., Ishizuka, 0., Nishizawa, 0.
  • Journal Title: LUTS Volume: 2 Pages: 76-82
  • Peer Reviewed
• [Journal Article] The microenvironment of freeze-injured mouse urinary bladders enables successful tissue engineering. (2009)
  • Author(s): Imamura, T., Yamamoto, T., Ishizuka, O., Gotoh, M., Nishizawa, O.
  • Journal Title: Tissue Eng. 15(11) Pages: 3367-3375
  • NAID: 120007110471
  • Peer Reviewed
• [Journal Article] The microenvironment of freeze-injured mouse urinary bladders enables successful tissue engineering. (2009)
  • Author(s): Imamura, T., Yamamoto, T., Ishizuka, O., Goto, M., Nishizawa, O.
  • Journal Title: Tissue Eng. 15 Pages: 3367-3375
  • NAID: 120007110471
  • Peer Reviewed
• [Journal Article] An Extract (THC-002) of Ba-Wei-Die-Huang-Wan inhibits expression of tachykinins and P2X3 and TRPV1 receptors and inihibits ATP-induced detrusor overactivity in spontaneously hypertensive rats. (2009)
  • Author(s): Imamura, T., Ishizuka, O., Zhong, C., Ogawa, T., Nakayama, T., Kurizaki, Y., Tanabe, T., Nishizawa, O., Andersson, K.-E.
  • Journal Title: Neurourol.Urodyn. 28 Pages: 529-534
  • Peer Reviewed
• [Journal Article] Over-expression of CXCR-3-binding chemokines and TNFSF14 in bladder urothelium from patients with ulcerative interstitial cystitis. (2009)
  • Author(s): Ogawa, T., Nishizawa, O., Chen, Z., Ishizuka, O., Seki, S., Imamura, T., Akahane, S., Honma, T., Honma, Y.
  • Journal Title: J.Urol. 183 Pages: 1206-1212
  • Peer Reviewed
• [Journal Article] Implantation of mouse bone marrow cells reconstructs layered smooth muscle structures in injured urinary bladder. (2008)
  • Author(s): Imamura, T., Kinebuchi, Y., Ishizuka, O., Seki, S., Igawa, Y., Nishizawa, O.
  • Journal Title: Cell transplantation 17 Pages: 267-278
  • Peer Reviewed
• [Journal Article] Implantation of mouse bone marrow cells reconstructs layered smooth muscle structures in injured urinary bladder (2008)
  • Author(s): Imamura, T., Kinebuchi, Y., Ishizuka, O., Seki, S., Igawa, Y., Nishizawa, O.
  • Journal Title: Cell transplantation 17 Pages: 267-278
  • Peer Reviewed
• [Journal Article] Gosha-jinki-gan reduces transmitter proteins and sensory receptors associated with C-fiber activation induced by acetic acid in ratbladder (2008)
  • Author(s): Imamura, T., Ishizuka, O., Aizawa, N., Zhong, C., Ogawa, T., Nakayama, T., Tanabe, T, . Nishizawa, O.
  • Journal Title: Neurouro Urodyn. 27 Pages: 832-837
  • Peer Reviewed
• [Journal Article] Effects of natriuretic peptides on intracavernous pressure and biood pressure in conscious rats (2008)
  • Author(s): Aizawa, N., Ishizuka, O., Ogawa, T., Mizusawa, H., Igawa, Y., Nishizawa, O., Anderssson K. E.
  • Journal Title: J. Sex. Med. 5 Pages: 2312-2317
  • Peer Reviewed
• [Presentation] Saline loading caused the change of the expression of al-adrecocepter subtypes , angiotensin 2 type 1 receptors in the prosate of spontanelusly hypertensive rats. (2010)
  • Author(s): Ishizuka, O., Nakayama,. T., Kurizaki, Y., Igawa, Y., Nishizawa, 0., Andersson, K.-E.
  • Organizer: The 40＾<th> Annual Meeting of the International Continence Society
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2010-08-23
• [Presentation] Implantation of human amniotic mesenchymal cells promotes morphological and functional recovery of the freeze-injured mouse urinary bladder. (2010)
  • Author(s): O Minagawa, T., Imamura, T., Aizawa, N., Ishizuka, O., Igawa, Y., Nishizawa, O.
  • Organizer: 25^<th> EAU 2010.
  • Place of Presentation: Barcelona.
• [Presentation] Cold stress stimulation induced urinary frequency is triggered by skin Transient Receptor Potential Melastatin (TRPM) 8, and suppressed by intravenous alpha 1 adrenoceptor antagonists in normal conscious rats. (2009)
  • Author(s): Ishizuka, O., Chen, Z., Imamura, T., Kurizaki, Y., Ishikawa, M., Nakayama, Igawa, Y., Nishizawa, O., Andersson, K.E.
  • Organizer: 30^<th> congress of the Societe Internationale d' Urologie
  • Place of Presentation: 上海、中国
  • Year and Date: 2009-11-02
• [Presentation] Mice freeze-injured urinary bladders provide a microenvironment for bone ?marrow-derived cells to regenerate smooth muscle layers. (2009)
  • Author(s): Imamura, T., Yamamoto, T., Ishizuka,O., Gotoh, M., Nishizawa,O.
  • Organizer: The 4^<th> Pan-pacific continence society meeting.
  • Place of Presentation: Fukuoka
  • Year and Date: 2009-09-10
• [Presentation] Possibility of regeneration of urinary bladder and urethral sphincter by own bone marrow cell. (2009)
  • Author(s): Ishizuka, O.
  • Organizer: 2009 Asia-European International Academic Forum for Urethral Reconstruction.
  • Place of Presentation: Shanghai.
  • Year and Date: 2009-02-06
• [Presentation] 骨髄細胞移植による膀胱平滑筋再生を可能にする生体微少環境についての検討 (2008)
  • Author(s): 今村哲也, 山本徳則, 石塚修, 小川輝之, 井川靖彦, 後藤百万, 西澤理
  • Organizer: 第5回泌尿器科再建再生研究会
  • Place of Presentation: 仙台
  • Year and Date: 2008-06-28
• [Book] 泌尿器検査のここがポイント (2010)
  • Author(s): 石塚修、西澤理
  • Publisher: 医学書院
• [Book] 夜間頻尿診療ガイドライン (2009)
  • Author(s): 石塚修、鈴木康之、武井実根雄、西澤理
  • Total Pages: 83
  • Publisher: ブラックウェル
• [Book] よくわかって役に立つ前立腺肥大症のすべて (2008)
  • Author(s): 石塚修, 西澤理
  • Publisher: 永井書店