Identification of genes affecting the efficiency of genetic transformation in wheat.
Project/Area Number |
20780007
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Breeding science
|
Research Institution | National Agricultural Research Organization |
Principal Investigator |
ABE Fumitaka National Agricultural Research Organization, 作物研究所麦類遺伝子技術研究チーム, 主任研究員 (30370547)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 植物分子育種 / 形質転換 / 網羅的遺伝子発現解析 / 形質転換効率 / コムギ / 組織培養 / 網羅的発現解析 / 軽質転換効率 |
Research Abstract |
In order to improve the system of genetic transformation in wheat, gene expression patterns of wheat cultured cells through transformation process were studied. Wheat cultivar "Bobwhite" which is the most suitable cultivar to transform was used as plant material. The change of gene expression levels caused by improved condition of transformation was analyzed using microarray. Expression variation of selected genes was compared with two varieties, Bobwhite and Nourin-61, by RT-PCR.
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Report
(4 results)
Research Products
(2 results)