The search for the candidate molecule for the vaccine to control a wide range of ectoparasite species of chickens

• Project/Area Number: 20H03137
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Kazuhiko 北海道大学, 獣医学研究院, 特任教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): 村田 史郎 北海道大学, 獣医学研究院, 准教授 (10579163) ; 今内 覚 北海道大学, 獣医学研究院, 教授 (40396304)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2020: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: ワクモ / トリサシダニ / ワクチン

Outline of Research at the Start

ワクモやトリサシダニは、鳥類の代表的な外部寄生虫であり、養鶏産業に大きな被害をもたらしている。現在は駆虫剤により防除されているが、薬剤耐性個体の出現や駆虫剤残存による衛生環境の悪化などが問題となり、抗ダニワクチンなど新規防除法の開発が必要である。そこで、ワクモとトリサシダニやミナミトリサシダニを同時に防除できるダニ種横断的な新規“ユニバーサル”ワクチンの開発を目指して、次世代シークエンサーを用いたRNA-SEQ解析や全ゲノム解析と相同性検索により、ダニ種間で共通の非暴露型抗原を探索・同定し、吸血したダニ個体に致死的に作用して鶏舎内の寄生虫個体数を持続的に減少できるワクチンへの応用を検討する。

Outline of Final Research Achievements

For the development of a "universal" vaccine, which can broadly and effectively control different mite species, such as poultry red mites (PRM) of the chicken, the search for candidate molecules for the vaccine was made by comprehensive analysis of expressed genes using RNA-SEQ. Several molecules, such as ferritin 2, were identified, and these molecules showed cross-reactivity among the mite species. Next, recombinant proteins derived from each mite species were prepared for each of the candidate molecules identified, and antisera were produced by immunizing chickens with those recombinant proteins. The in vitro feeding assay showed that a significant decrease in survival rate was observed in PRMs which were fed with the antisera compared to the control-fed PRMs.

Academic Significance and Societal Importance of the Research Achievements

ワクモやトリサシダニなどは、鳥類の代表的な外部寄生虫であり、養鶏産業に大きな経済的被害をもたらしている。現在、その防除には主に駆虫剤が使用されているが、薬剤耐性個体の出現や駆虫剤残存による衛生環境の悪化などが問題となっており、抗ダニワクチンなど新規防除法の開発が必要となっている。本研究で同定した分子は、有効なワクチン抗原として、ワクチン接種鶏で吸血し異なるダニ種の個体に致死的に作用して、鶏舎内の寄生虫個体数を持続的に減少できるダニ種横断的な新規“ユニバーサル”ワクチンの開発に応用可能であり、養鶏産業の持続的な発展に寄与できる。

Research Products

• [Journal Article] Suppressive modulation of host immune responses by Dermanyssus gallinae infestation. (2023)
  • Author(s): Fujisawa, S., Murata, S., Isezaki, M., Win, S-Y., Sato, T., Oishi, E., Taneno, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Poultry Science Volume: 102 Issue: 4 Pages: 102352-102352
  • DOI: 10.1016/j.psj.2023.102532
  • Peer Reviewed / Open Access
• [Journal Article] Potential of ferritin 2 as an antigen for the development of a universal vaccine for avian mites, poultry red mites, tropical fowl mites, and northern fowl mites. (2023)
  • Author(s): Win, S. Y., Murata, S., Fujisawa, S., Seo, H., Sato, J., Motai, Y., Sato, T., Oishi, E., Taneno, A., Htun, L. L., Bawm, S., Okagawa, T., Maekawa, N., Konnai, S., Ohashi, K.
  • Journal Title: Frontiers in Veterinary Science Volume: 印刷中 Pages: 1182930-1182930
  • DOI: 10.3389/fvets.2023.1182930
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Redox imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line (2022)
  • Author(s): Della Noce B, Martins da Silva R, de Carvalho Uhl MV, Konnai S, Ohashi K, Calixto C, Arcanjo A, de Abreu LA, de Carvalho SS, da Silva Vaz I Jr, Logullo C.
  • Journal Title: J Biol Chem. Volume: 18 Issue: 3 Pages: 101599-101599
  • DOI: 10.1016/j.jbc.2022.101599
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens (2022)
  • Author(s): Ariizumi, T., Murata, S., Fujisawa, S., Isezaki, M., Sato, T., Oishi, E., Taneno, A., Ichii, O., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Poultry Science Volume: 101 Issue: 3 Pages: 101638-101638
  • DOI: 10.1016/j.psj.2021.101638
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen (2021)
  • Author(s): Fujisawa Sotaro、Murata Shiro、Isezaki Masayoshi、Ariizumi Takuma、Sato Takumi、Oishi Eiji、Taneno Akira、Maekawa Naoya、Okagawa Tomohiro、Ichii Osamu、Konnai Satoru、Ohashi Kazuhiko
  • Journal Title: Parasitology Volume: 149 Issue: 1 Pages: 105-115
  • DOI: 10.1017/s0031182021001608
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of a novel cysteine protease inhibitor from poultry red mites: potential vaccine for chickens (2021)
  • Author(s): Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K.
  • Journal Title: Vaccines (Basel) Volume: 9 Issue: 12 Pages: 1472-1472
  • DOI: 10.3390/vaccines9121472
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens (2021)
  • Author(s): Murata, S., Taniguchi, A., Isezaki, M., Fujisawa, S., Sakai, E., Taneno, A., Ichii, O., Ito, T., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasite Volume: 28 Pages: 9-9
  • DOI: 10.1051/parasite/2021005
  • Peer Reviewed / Open Access
• [Journal Article] Selection of reference genes for quantitative PCR analysis in poultry red mite (<i>Dermanyssus gallinae</i>) (2021)
  • Author(s): Ariizuim, T., Murata, S., Fujisawa, S., Isezaki, M., Maekawa, N., Okagawa, T., Sato, T., Oishi, E., Taneno, A., Konnai, S., Ohashi, K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 4 Pages: 558-565
  • DOI: 10.1292/jvms.20-0677
  • NAID: 130008023262
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome dynamics of blood-fed and starved poultry red mites, Dermanyssus gallinae (2020)
  • Author(s): Fujisawa, S., Murata, S., Isezaki, M., Oishi, E., Taneno, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasitology International Volume: 78 Pages: 102156-102156
  • DOI: 10.1016/j.parint.2020.102156
  • NAID: 120007160301
  • Peer Reviewed
• [Presentation] ワクモ（Dermanyssus gallinae）由来シスタチン様分子の抗ワクモワクチン抗原としての評価 (2022)
  • Author(s): 藤澤宗太郎、村田史郎、伊勢崎政美、有泉拓馬、佐藤匠、大石英司、種子野章、前川直也、岡川朋弘、市居修、今内覚、大橋和彦
  • Organizer: 第165回 日本獣医学会
• [Presentation] Characterization of cysteine proteases from avian mites to develop a vaccine with broad efficacy (2022)
  • Author(s): Win Shwe Yee、村田史郎、藤澤宗太郎、瀬尾光里、佐藤純平、佐藤匠、大石英司、種子野章、岡川朋弘、前川直也、今内覚、大橋和彦
  • Organizer: 第165回 日本獣医学会
• [Presentation] ワクモ由来の2種類のアスパラギン酸ペプチダーゼの性状解析 (2022)
  • Author(s): 瀬尾光里、村田史郎、Shwe Yee Win、藤澤宗太郎、北條巧、伊勢崎政美、佐藤匠、大石英司、種子野章、市居修、前川直也、岡川朋弘、今内覚、大橋和彦
  • Organizer: 第165回 日本獣医学会
• [Presentation] ワクモ(Dermanyssus gallinae)の吸血に対する宿主免疫応答の解明 (2021)
  • Author(s): 藤澤宗太郎、村田史郎、伊勢崎政美、佐藤匠、大石英司、種子野章、 前川直也、岡川朋弘、今内覚、大橋和彦
  • Organizer: 第164回日本獣医学会
• [Presentation] ワクモ( Dermanyssus gallinae )由来システインプロテアーゼの抗ワクモワクチン抗原とし ての評価 (2021)
  • Author(s): 有泉拓馬、村田史郎、藤澤宗太郎、伊勢崎政美、佐藤匠、大石英司、 種子野章、市居修、前川直也、岡川朋弘、今内覚、大橋和彦
  • Organizer: 第164回日本獣医学会
• [Presentation] ワクモにおける定量的PCR法確立のための内在性コントロール遺伝子の探索 (2020)
  • Author(s): 有泉拓馬、村田史郎、藤澤宗太郎、伊勢崎政美、前川直也、岡川朋弘、種子野章、大石英司、今内覚、大橋和彦
  • Organizer: 第163回日本獣医学会