Studies on Physiological Significance of Cytoskeleton Redistribution in the Mammalian Eggs and Its Signal Transduction Pathways

• Project/Area Number: 21580340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Hirosaki University
• Principal Investigator: SUZUKI Hiroyuki 弘前大学, 農学生命科学部, 教授 (50211313)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 哺乳動物 / 卵母細胞 / 卵成熟 / 細胞骨格 / シグナル伝達 / 微小管 / マイクロフィラメント / 中間径線維 / ネスチン / ビメンチン / 中間径フィラメント / ケラチン / ミオシン / キネシン

Research Abstract

Function of cytoskeleton in the mammalian oocytes during maturation was examined. Results revealed that microfilaments and microtubules may be differently involved in the mitochondrial redistribution according to the interphase and M-phase of the cell cycle. Dynamics of the motor proteins, myosin and kinesin, during oocyte maturation was also clarified. Furthermore, the distributional changes of intermediate filament proteins keratin, vimentin and nestin were shown. Effects of the specific inhibitors of the cell-cycle regulating factors cdc2 and MAP kinase kinase or Rho kinase inhibition on the cytoskeletal architecture were evaluated.

Research Products

• [Journal Article] Cell cycle-dependent dynamics of cytoskeleton involving mitochondrial redistribution in hamster embryos (2012)
  • Author(s): Kabashima, K., Yoshinaga, D., Fang, J., Matsuzaki, M. and Suzuki, H.
  • Journal Title: Reprod. Dom. Anim Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Cell cycle-dependent dynamics of cytoskeleton involving mitochondrial redistribution in hamster embryos (2012)
  • Author(s): Kabashima, K., Yoshinaga, D., Fang, J., Matsuzaki, M., Suzuki, H.
  • Journal Title: Reproduction in Domestic Animals Volume: 47(印刷中)
  • Peer Reviewed
• [Journal Article] Temporary Inhibition of Germinal Vesicle Breakdown by Rho kinase Inhibitor Y-27632 is Detrimental to Oocyte Maturation (2011)
  • Author(s): Suzuki, H., Koyama, K., Kabashima, K. Fang, J. and Matsuzaki, M
  • Journal Title: J. Mamm. Ova Res Volume: Vol.28 Pages: 126-130
  • NAID: 10029898493
  • Peer Reviewed
• [Journal Article] Temporary Inhibition of Germinal Vesicle Breakdown by Rho Kinase Inhibitor Y-27632 is Detrimental to Oocyte Maturation (2011)
  • Author(s): Suzuki, H., Koyama, K., Kabashima, K., Fang, J., Matsuzaki, M.
  • Journal Title: Journal of Mammalian Ova Research Volume: 28 Issue: 3 Pages: 126-130
  • DOI: 10.1274/jmor.28.126
  • NAID: 10029898493
  • ISSN: 1341-7738, 1347-5878
  • Peer Reviewed
• [Journal Article] Intermediate filament keratin dynamics during oocyte maturation requires MPF and MAP kinase activities in the hamster (2010)
  • Author(s): Kabashima, K., Matsuzaki, M. and Suzuki, H.
  • Journal Title: Reprod. Dom. Anim Volume: Vol.45
  • Peer Reviewed
• [Journal Article] Ghrelin and leptin did not improve meiotic maturation of porcine oocytes cultured in vitro (2010)
  • Author(s): Suzuki, H., Sasaki, Y., Shimizu, M., Matsuzaki, M., Hashizume, T. and Kuwayama, H.
  • Journal Title: Reprod. Dom. Anim Volume: Vol.45 Pages: 927-930
  • Peer Reviewed
• [Journal Article] Ghrelin and leptin did not improve meiotic maturation of porcine oocytes cultured in vitro. (2010)
  • Author(s): Suzuki, H., Sasaki, Y., Shimizu, M., Matsuzaki, M., Hashizume, T., Kuwayama, H.
  • Journal Title: Reproduction in Domestic Animals Volume: 45(5) Pages: 927-930
  • Peer Reviewed
• [Journal Article] Intermediate Filament Keratin Dynamics During Oocyte Maturation Requires Maturation/M-Phase Promoting Factor and Mitogen-Activated Protein Kinase Kinase Activities in the Hamster (2010)
  • Author(s): Kabashima, K., Matsuzaki, M., Suzuki, H.
  • Journal Title: Reproduction in Domestic Animals Volume: 45(5)
  • Peer Reviewed
• [Journal Article] Ghrelin and leptin did not improve meiotic maturation of porcine oocytes cultured in vitro. (2009)
  • Author(s): Suzuki, H., Sasaki, Y., Shimizu, M., Matsuzaki, M., Hashizume, T., Kuwayama, H.
  • Journal Title: Reproduction in Domestic Animals Mar.31
  • Peer Reviewed
• [Journal Article] Intermediate Filament Keratin Dynamics During Oocyte Maturation Requires Maturation/M-Phase Promoting Factor and Mitogen-Activated Protein Kinase Kinase Activities in the Hamster (2009)
  • Author(s): Kabashima, K., Matsuzaki, M., Suzuki, H.
  • Journal Title: Reproduction in Domestic Animals Nov.17
  • Peer Reviewed
• [Presentation] ハムスター卵成熟過程におけるミトコンドリアの再配置は,微小管ではなくアクチン繊維によって制御される (2012)
  • Author(s): 椛嶋克哉,房家.,松崎正敏,鈴木裕之
  • Organizer: 日本哺乳動物卵子学会第53回大会
  • Place of Presentation: 千里ライフサイエンスセンター
• [Presentation] ブタ卵母細胞の成熟に伴う中間径繊維,ケラチンとビメンチンの分布変化 (2011)
  • Author(s): 小田純平, 椛嶋克哉, 加藤愛子, 房家〓, 松崎正敏, 鈴木裕之
  • Organizer: 東北畜産学会第61回大会
  • Place of Presentation: 青森市ねぶたの家「ワ・ラッセ」
  • Year and Date: 2011-09-08
• [Presentation] ブタ卵母細胞の成熟に伴う中間径繊維,ケラチンとビメンチンの分布変化 (2011)
  • Author(s): 小田純平,椛嶋克哉,加藤愛子,房家〓,松崎正敏,鈴木裕之
  • Organizer: 東北畜産学会第61回大会
  • Place of Presentation: 青森市
• [Presentation] ハムスター初期胚における細胞周期依存的なミトコンドリア分布の制御機構 (2011)
  • Author(s): 椛嶋克哉,吉永大輔,房家.,松崎正敏,鈴木裕之
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 北里大学
• [Presentation] 日本繁殖生物学会第103回大会 (2010)
  • Author(s): 椛嶋可哉, 山内康一,松崎正敏, 鈴木裕之
  • Organizer: ハムスター初期胚におけるケラチンの時空間的分布変化
  • Place of Presentation: 北里大学
  • Year and Date: 2010-09-02
• [Presentation] Rhoキナーゼは卵核胞崩壊と第一極体の放出に関与する (2010)
  • Author(s): 古山敬一, 椛嶋克哉, 松崎正敏, 鈴木裕之
  • Organizer: 日本哺乳動物卵子学会第51回大会
  • Place of Presentation: 新潟市朱鷺メッセ
  • Year and Date: 2010-05-30
• [Presentation] ハムスター初期胚におけるケラチンの時空間的分布変化 (2010)
  • Author(s): 椛嶋克哉・山内浩一・松崎正敏・鈴木裕之
  • Organizer: 日本繁殖生物学会第103回大会
  • Place of Presentation: 北里大学
• [Presentation] Rhoキナーゼは卵核胞崩壊と第一極体の放出に関与する (2010)
  • Author(s): 古山敬一・椛嶋克哉・松崎正敏・鈴木裕之
  • Organizer: 日本哺乳動物卵子学会第51回大会
  • Place of Presentation: 朱鷺メッセ
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.ac.jp/lab/3/animsci/reference.html
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.ac.jp/lab/3/animsci/reference.html
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.acjp//ab/3/animsci/reference.html
• [Remarks]
  • URL: http://nature.cc.hirosaki-u.ac.jp/lab/3/animsci/reference.html