Elucidation of mechanisms of aggravation in Influenza virus encephalopathy using with tissue culture system

• Project/Area Number: 21591330
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fukushima Medical University
• Principal Investigator: HOSOYA Mistuaki 福島県立医科大学, 医学部, 教授 (80192318)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Yukihiko 福島県立医科大学, 医学部, 准教授 (00305369) ; HASHIMOTO Koichi 福島県立医科大学, 医学部, 准教授 (50322342)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小児感染症学 / インフルエンザ脳症 / アポトーシス / 血管内皮細胞 / サイトカイン / 細胞透過性 / タイトジャンクション / ヒト臍帯静脈血管内皮細胞 / TNF-α / 経上皮内皮電気抵抗値 / ウェスタンプロット / RNA / 消炎鎮痛剤 / MTT法 / 細胞毒性 / インフルエンザ / 脳症

Research Abstract

We examined induction of apoptosis, cytotoxicity, and change of tight junction due to TNF-α and non-steroidal anti-inflammatory drugs in model of Influenza virus encephalopathy using with human umbilical vein vascular endothelial cell(HUVEC). We evaluated influence of TNF-α to tight junction of HUVEC by western blot for Claudin-5 and Occludin, and by measurement of trans-endothelial epithelium endodermis electric resistance(TER) for alteration of cell permeability.

Research Products

• [Journal Article] Rhinovirus load and disease severity in children with lower respiratory tract infections (2012)
  • Author(s): Takeyama A, Hashimoto K, Sato M, Sato T, Kanno S, Takano K, Ito M, Katayose M, Nishimura H, Kawasaki Y, Hosoya M
  • Journal Title: J Med Virol Volume: 84巻 Pages: 1135-42
  • Peer Reviewed
• [Journal Article] Mumps Virus-Associated Acute Encephalopathy : Case Report and Review of the Literature (2012)
  • Author(s): Watanabe M, Suyama K, Hashimoto K, Sato M, Ohara S, Abe Y, Kawasaki Y, Yamaguchi S, Saijo M, Hosoya M
  • Journal Title: J Child Neurol
  • Peer Reviewed
• [Journal Article] Survey of subacute sclerosing panencephalitis in Japan. (2012)
  • Author(s): Abe Y, Hashimoto K, Iinuma K, Ohtsuka Y, Ichiyama T, Kusuhara K, Nomura K, Mizuguchi M, Aiba H, Suzuki Y, Mizusawa H, Hosoya M.
  • Journal Title: J Child Neurol.
• [Journal Article] Survey of Subacute Sclerosing Panencephalitis in Japan (2012)
  • Author(s): Abe Y, Hosoya M, et al
  • Journal Title: J Child Neurol Volume: (In press)
  • Peer Reviewed
• [Journal Article] 亜急性硬化性全脳炎に対する治療の動向 (2011)
  • Author(s): 細矢光亮
  • Journal Title: 最新医学 Volume: 66巻 Pages: 86-90
• [Journal Article] 亜急性硬化性全脳炎(SSPE)に対する新たな治療法の開発を目指して (2011)
  • Author(s): 細矢光亮
  • Journal Title: Neuroinfection Volume: 16巻 Pages: 1-8
• [Journal Article] Subacute sclerosing panencephalitis (SSPE) (2011)
  • Author(s): Hosoya M
  • Journal Title: Rinsho Shinkeigaku Volume: 51 Pages: 1047-50
  • NAID: 130004504817
• [Journal Article] Uteroglobulin-related protein 1 and severity of respiratory syncytial virus infection in children admitted to hospital (2011)
  • Author(s): Hashimoto K, et al
  • Journal Title: J Med Virol Volume: 83 Issue: 6 Pages: 1086-92
  • DOI: 10.1002/jmv.22073
  • Peer Reviewed
• [Journal Article] Serum SP-D levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis. (2011)
  • Author(s): Kawasaki Y, Hosoya M, et al.
  • Journal Title: Pediatr Pulmonol. Volume: 46 Pages: 18-22
  • Peer Reviewed
• [Journal Article] Glucocorticoid receptor expression and cortisol level in cord blood of term infants. (2011)
  • Author(s): Imamura T, Hosoya M, et al.
  • Journal Title: J Matern Fetal Neonatal Med.
  • Peer Reviewed
• [Journal Article] The trend of drug-resistant Streptococcus pneumoniae from nasopharynx of children. (2011)
  • Author(s): Matsumoto A, Hosoya M, et al.
  • Journal Title: J Infect Chemother. Volume: 16 Pages: 255-259
  • NAID: 10027190310
  • Peer Reviewed
• [Presentation] 最近のワクチンの動向 (2012)
  • Author(s): 細矢光亮
  • Organizer: 第19回群馬小児薬物医療研究会
  • Place of Presentation: 前橋市
  • Year and Date: 2012-02-22
• [Presentation] 中枢神経感染症トピックス (2012)
  • Author(s): 細矢光亮
  • Organizer: 第11回大阪小児感染免疫カンファレンス
  • Place of Presentation: 大阪市
  • Year and Date: 2012-01-21
• [Presentation] インフルエンザウイルス感染症について (2012)
  • Author(s): 細矢光亮
  • Organizer: 第1回いわて感染症を考える会
  • Place of Presentation: 盛岡市
  • Year and Date: 2012-01-19
• [Presentation] ヌードマウス脳内に持続した麻疹ウイルスの検討 (2011)
  • Author(s): 阿部優作、橋本浩一、細矢光亮
  • Organizer: 第16日本神経感染症学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-04
• [Presentation] ヌードマウス脳内に持続感染した麻疹ウイルスの検討 (2011)
  • Author(s): 阿部優作、橋本浩一、川崎幸彦、細矢光亮
  • Organizer: 第43回日本小児感染症学会総会学術講演会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-10-30
• [Presentation] 膜融合蛋白(F蛋白)を標的とした新規ペプチドによる亜急性硬化性全脳炎の新たな治療戦略 (2011)
  • Author(s): 渡部真裕、阿部優作、橋本浩一、川崎幸彦、細矢光亮
  • Organizer: 第43回日本小児感染症学会総会学術講演会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-10-30
• [Presentation] インフルエンザの合併症とその予防・治療 (2011)
  • Author(s): 細矢光亮
  • Organizer: 第43回日本小児感染症学会総会・学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-10-30
• [Presentation] There is no association between the-112G/A polymorphism of UGRP1 and serum UGRP1, and severity of RSV infection in Hospitalized children. (2010)
  • Author(s): Hashimoto K, Hosoya M, et al.
  • Organizer: 第7回RSウイルス国際シンポジウム2010
  • Place of Presentation: ロッテルダム
  • Year and Date: 2010-12-03
• [Book] 単純ヘルペス脳炎診療ガイドライン. In:五十嵐隆/塩見正司・編小児科診療ピクシス「急性脳炎・急性脳症」 (2011)
  • Author(s): 細矢光亮
  • Publisher: 中山書店
• [Book] 小児科診療ピクシス「急性脳炎・急性脳症」 (2011)
  • Author(s): 細矢光亮
  • Total Pages: 4
  • Publisher: 中山書店,94-97頁,2011年
• [Book] 小児科臨床ピクシス 小児感染症 (2011)
  • Author(s): 細矢光亮
  • Publisher: 中山書店