Lambda-DSLM, a new light-sheet microscope for spectroscopy
| Project/Area Number |
21770177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
NONAKA Shigenori National Institute for Basic Biology, 時空間制御研究室, 准教授 (90435529)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 発生・分化 / 細胞・組織 / 共焦点顕微鏡 |
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| Research Abstract |
Light-sheet microscopy including Digital Scanned Light-sheet Microscope (DSLM) has advantages with its deep penetration and low photo-toxicity. We have developed a new microscope system combining spectroscopy with DSLM, namely lambda-DSLM. So far we have confirmed that the system has enough spectral resolution for linear unmixing or fluorescence or raman imaging, via analyses using fluorescent beads or emission lines from light sources. Imaging of biological specimen expressing fluorescent proteins and development of an integrated software for device control and image reconstruction are ongoing.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II2010
Author(s)
Yuki Hirota, Alice Meunier, Shihhui Huang, Togo Shimozawa, Osamu Yamada, Yasuyuki S.Kida, Masashi Inoue, Tsubasa Ito, Hiroko Kato, Masanori Sakaguchi, Takehiko Sunabori, Masa-aki Nakaya, Shigenori Nonaka, Toshihiko Ogura, Hideo Higuchi, Hideyuki Okano, Nathalie Spassky, Kazunobu Sawamoto
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Journal Title
Development
Volume: 137
Pages: 3037-3046
Related Report
Peer Reviewed
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[Journal Article] Planar polarization of node cells determines the rotational axis of node cilia2010
Author(s)
M.Hashimoto, K.Shinohar, J.Wang, S.Ikeuchi, S.Yoshiba, C.Meno, S.Nonaka, S.Takada, K.Hatta, A.Wynshaw-Boris, H.Hamada
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Journal Title
Nature Cell Biology 12
Pages: 170-176
Related Report
Peer Reviewed
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[Presentation] Live imaging of the whole mouse embryo during gastrulation2010
Author(s)
Takehiko Ichikawa, Philipp Keller, Ernst Stelzer, Shigenori Nonaka "Live imaging of the whole mouse embryo during gastrulation
Organizer
3rd Annual Meeting for the Japanese Society of Developmental Biologists Jointly Sponsored by the Asia-Pacific Developmental Biology Network
Place of Presentation
京都国際会館(京都市)
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