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Role of nucleoporin Nup98 in the nuclear differentiation process of ciliated protozoa

Research Project

Project/Area Number 21770224
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

IWAMOTO Masaaki  National Institute of Information and Communications Technology, 未来ICT研究センター・バイオICTグループ, 専攻研究員 (80450683)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords核膜孔複合体 / ヌクレオポリン / 核分化 / 接合 / テトラヒメナ / 細胞核 / 核膜孔
Research Abstract

The macronucleus (MAC) and the micronucleus (MIC) of ciliate Tetrahymena thermophila can be distinguished by nucleus-specific homologues of nucleoporin Nup98. In this study, we examined the dynamic behavior of the Nup98s during nuclear differentiation. We observed that MAC-type Nup98 started to assemble on the periphery of the future MACs immediately after the end of the last mitotic division of postzygotic nuclei. This timing was about 30 min earlier than the nuclear transport of factors required for MAC nuclear differentiation. These results suggest that placement of the MAC NPC containing MAC-type Nup98 is the first distinct event triggering further MAC differentiation, probably by leading to MAC-selective nuclear transport.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(43 results)

All 2011 2010 2009 Other

All Journal Article Presentation Remarks Patent(Industrial Property Rights)

  • [Journal Article] Identification and characterization of nuclear pore complex components in Arabidopsis thaliana.2010

    • Author(s)
      Tamura, K., Fukao, Y., Iwamoto, M., Haraguchi, T., Hara-Nishimura, I.
    • Journal Title

      The Plant Cell 22

      Pages: 4084-4097

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nucleoporin Nup98 : a gatekeeper in the eukaryotic kingdoms.2010

    • Author(s)
      Iwamoto, M., Asakawa, H., Hiraoka, Y., Haraguchi, T.
    • Journal Title

      Genes to Cells 15

      Pages: 661-669

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The nucleoporin Nup98 : a gatekeeper in the eukaryotic kingdoms2010

    • Author(s)
      Iwamoto, M.
    • Journal Title

      Genes Cells

      Volume: 15 Pages: 661-669

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Characterization of Nuclear Pore Complex Components in Arabidopsis thaliana2010

    • Author(s)
      Tamura, K.
    • Journal Title

      The Plant Cell

      Volume: 22 Pages: 4084-4097

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate Tetrahymena.2009

    • Author(s)
      Iwamoto, M., Mori, C., Kojidani, T., Bunai, F., Hori, T., Fukagawa, T., Hiraoka, Y., Haraguchi T.
    • Journal Title

      Current Biology 19

      Pages: 843-847

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 核膜バリアの意味:その破綻によってみえてきた核膜孔複合体の機能2009

    • Author(s)
      岩本政明, 原口徳子
    • Journal Title

      実験医学増刊号「細胞核:遺伝情報制御と疾患」 27

      Pages: 79-86

    • Related Report
      2010 Final Research Report
  • [Journal Article] Two Distinct Repeat Sequences of Nup98 Nucleoporins Characterize Dual Nuclei in the Binucleated Ciliate Tetrahymena.2009

    • Author(s)
      Masaaki Iwamoto
    • Journal Title

      Current Biology 19

      Pages: 843-847

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 核膜バリアの意味:その破綻によって見えてきた核膜孔複合体の機能2009

    • Author(s)
      岩本政明
    • Journal Title

      実験医学 27(17)

      Pages: 2759-2766

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nuclear transport machineries cooperatively working with the nucleus-specific nucleoporins in the bi-nucleated protozoan Tetrahymena thermophila -Implication of the nuclear transport system in evolution of eukaryotes?.2011

    • Author(s)
      岩本政明, 森知栄, 平岡泰, 原口徳子
    • Organizer
      The 20th CDB Meeting-Molecular Bases for Evolution of Complex Traits
    • Place of Presentation
      理化学研究所(神戸市)
    • Year and Date
      2011-02-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Nuclear transport Machineries Cooperatively Working with the Nucleus-specific Nucleoporins in the Bi-nucleated Protozoan Tetrahymena thermophila-Implication of the Nuclear Transport System in Evolution of Eukaryotes-2011

    • Author(s)
      Iwamoto, M.
    • Organizer
      The 20th CDB Meeting "Molecular Bases for Evolution of Complex Traits"
    • Place of Presentation
      理化学研究所、兵庫県
    • Year and Date
      2011-02-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynamics of the nuclear pore complexes and the nuclear envelope during nuclear differentiation in Tetrahymena thermophila.2011

    • Author(s)
      岩本政明, 武内史英, 荒神尚子, 小坂田裕子, 森知栄, 糀谷知子, 平岡泰, 原口徳子
    • Organizer
      遺伝情報場国際シンポジウム
    • Place of Presentation
      ウェスティン淡路(淡路市)
    • Year and Date
      2011-01-25
    • Related Report
      2010 Final Research Report
  • [Presentation] The Nuclear Pore Complex Determines Nuclear Differentiation in Ciliate Tetrahymena thermophila2011

    • Author(s)
      Haraguchi, T.
    • Organizer
      International Symposium on the Physicochemical Field for Genetic Activities
    • Place of Presentation
      淡路夢舞台国際会議場、兵庫県
    • Year and Date
      2011-01-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynamics of the Nuclear Pore Complexes and the Nuclear Envelope during Nuclear Differentiation in Tetrahymena thermophila2011

    • Author(s)
      Iwamoto, M.
    • Organizer
      International Symposium on the Physicochemical Field for Genetic Activities
    • Place of Presentation
      淡路夢舞台国際会議場、兵庫県
    • Year and Date
      2011-01-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynamics of the nuclear pore complexes and the nuclear envelope upon nuclear differentiation in ciliate Tetrahymena thermophila.2010

    • Author(s)
      岩本政明, 武内史英, 荒神尚子, 小坂田裕子, 森知栄, 糀谷知子, 平岡泰, 原口徳子
    • Organizer
      アメリカ細胞生物学会
    • Place of Presentation
      ペンシルベニアコンベンションセンター(Philadelphia, USA)
    • Year and Date
      2010-12-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamics of the Nuclear Pore Complexes and the Nuclear Envelope upon Nuclear Differentiation in ciliate Tetrahymena thermophila2010

    • Author(s)
      Iwamoto M.
    • Organizer
      The American Society for Cell Biology Annual Meeting
    • Place of Presentation
      Pennsylvania Convention Center U.S.A
    • Year and Date
      2010-12-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of micronucleus-specific nuclear localization signals in ciliate Tetrahymena.2010

    • Author(s)
      岩本政明, 森知栄, 桝田浩孝, 小布施力史, 平岡泰, 原口徳子
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Macronucleus-specific nuclear pore complex determines macronuclear differentiation in ciliate Tetrahymena thermophila2010

    • Author(s)
      武内史英
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド、兵庫県
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] テトラヒメナにおける小核特異的な核移行シグナルの同定2010

    • Author(s)
      岩本政明
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド、兵庫県
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] テトラヒメナの大核と小核の核膜孔複合体タンパク質の研究-繊毛虫が2核を使い分ける仕組みの解明に向けて-2010

    • Author(s)
      岩本政明
    • Organizer
      日本原生動物学会
    • Place of Presentation
      茨城大学(水戸市)(招待講演)
    • Year and Date
      2010-11-06
    • Related Report
      2010 Final Research Report
  • [Presentation] 繊毛虫テトラヒメナの核分化過程における核膜孔複合体のダイナミクス2010

    • Author(s)
      武内史英
    • Organizer
      第43回 日本原生動物学会大会
    • Place of Presentation
      茨城大学、茨城県
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] テトラヒメナの大核と小核の核膜孔複合体タンパク質の研究-繊毛虫が2核を使い分ける仕組みの解明に向けて-2010

    • Author(s)
      岩本政明
    • Organizer
      第43回 日本原生動物学会大会
    • Place of Presentation
      茨城大学、茨城県
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Live CLEM : An approach for observing molecular dynamics in high resolution2010

    • Author(s)
      Haraguchi, T.
    • Organizer
      日本顕微鏡学会第66回学術講演会
    • Place of Presentation
      名古屋国際会議場、愛知県
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynamics of nuclear envelope and nuclear pore complexes upon nuclear differentiation in ciliate Tetrahymena thermophila.2010

    • Author(s)
      岩本政明, 武内史英, 森知栄, 小坂田裕子, 糀谷知子, 荒神尚子, 平岡泰, 原口徳子
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 繊毛虫テトラヒメナの核分化における核膜と核膜孔複合体のダイナミクス2010

    • Author(s)
      岩本政明
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪国際会議場、大阪府
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 繊毛虫テトラヒメナの核分化と核膜孔複合体との関係2010

    • Author(s)
      原口徳子
    • Organizer
      第27回染色体ワークショップ
    • Place of Presentation
      御殿場高原時之栖、静岡県
    • Year and Date
      2010-01-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] テトラヒメナのNup98リピート領域に結合する核輸送関連因子の探索2009

    • Author(s)
      岩本政明, 小坂田裕子, 森知栄, 桝田浩孝, 小布施力史, 平岡泰, 原口徳子
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] テトラヒメナのNup98リピート領域に結合する核輸送関連因子の探索2009

    • Author(s)
      岩本政明
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] テトラヒメナの核選択的ヌクレオポリンNup98は核輸送のバリアとして働く2009

    • Author(s)
      岩本政明, 森知栄, 桝田浩孝, 小布施力史, 平岡泰, 原口徳子
    • Organizer
      日本原生動物学会
    • Place of Presentation
      石巻専修大学(石巻市)
    • Year and Date
      2009-11-01
    • Related Report
      2010 Final Research Report
  • [Presentation] テトラヒメナの核選択的ヌクレオポリンNup98は核輸送のバリアとして働く2009

    • Author(s)
      岩本政明
    • Organizer
      第42回原生動物学会
    • Place of Presentation
      石巻専修大学、宮城県
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Live CLEM : A new approach for observing molecular dynamics in high resolution2009

    • Author(s)
      Tokuko Haraguchi
    • Organizer
      6^<th> International Symposium on Electron Microscopy in Medicine and Biology 2009
    • Place of Presentation
      Centennial Hall, Kobe University, Hyogo, Japan
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nup98 nucleoporins bearing different types of repeat sequence characterize nucleus-specific nuclear pore complexes in Tetrahymena thermophila.2009

    • Author(s)
      岩本政明, 武内史英, 森知栄, 平岡泰, 原口徳子
    • Organizer
      FASEB Summer Research Conferences (Ciliate Molecular Biology)
    • Place of Presentation
      Vermont Academy (Vermont, USA)
    • Year and Date
      2009-07-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Nup98 nucleoporins bearing different types of repeat sequence characterizenucleus-specific nuclear pore complexes in Tetrahymena thermophila2009

    • Author(s)
      Masaaki Iwamoto
    • Organizer
      FASEB Summer Research Conferences "Ciliate Molecular Biology"
    • Place of Presentation
      Vermont Academy, Vermont, USA
    • Year and Date
      2009-07-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 遺伝子発現・収納を制御するセルバリアとしての核膜孔蛋白質Nup982009

    • Author(s)
      原口徳子
    • Organizer
      第8回核ダイナミクス研究会
    • Place of Presentation
      ラフォーレ修善寺、静岡県
    • Year and Date
      2009-06-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nup98 nucleoporins bearing different types of repeat sequence are involved in the nucleus-selective transport in the ciliate Tetrahymena.2009

    • Author(s)
      岩本政明, 森知栄, 武内史英, 平岡泰, 原口徳子
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2009-06-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 繊毛虫の大核・小核機能を保証する核膜孔複合体の構造とダイナミクス2009

    • Author(s)
      原口徳子
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋国際会議場、愛知県
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nup98 nucleoporins bearing different types of repeat sequence are involved in the nucleus-selective transport in the ciliate Tetrahymana2009

    • Author(s)
      Masaaki Iwamoto
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋国際会議場、愛知県
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dynamics and ultrastructure of the nuclear pore complexes during nuclear differentiation in Tetrahymena thermophila2009

    • Author(s)
      Fumihide Bunai
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋国際会議場、愛知県
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Live CLEM : A New Approach for Observing Molecular Dynamics in High Resolution2009

    • Author(s)
      Tokuko Haraguchi
    • Organizer
      The 9th NIBB-EMBL Symposium "Functional Imaging from Atoms to Organisms"
    • Place of Presentation
      Okazaki Conference Center, Aichi, Japan
    • Year and Date
      2009-04-20
    • Related Report
      2009 Annual Research Report
  • [Remarks] 報道発表「テトラヒメナ2種類の核 細胞が判別、原理解明 情通機構 IT技術に応用」日経産業新聞,平成21年5月13日掲載

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www-karc.nict.go.jp/w131103/CellMagic/index-J.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www2.nict.go.jp/w/w103/w131103/CellMagic/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www-karc.nict.go.jp/w131103/CellMagic/index.html

    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 顕微鏡観察用サンプル作製方法,及びそれに用いるキット2009

    • Inventor(s)
      武内史英, 岩本政明, 原口徳子, 平岡泰
    • Industrial Property Rights Holder
      独立行政法人情報通信研究機構
    • Patent Publication Number
      2009-229339
    • Acquisition Date
      2009-10-08
    • Related Report
      2010 Final Research Report

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Published: 2009-03-31   Modified: 2016-04-21  

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