Role of nucleoporin Nup98 in the nuclear differentiation process of ciliated protozoa
| Project/Area Number |
21770224
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
IWAMOTO Masaaki National Institute of Information and Communications Technology, 未来ICT研究センター・バイオICTグループ, 専攻研究員 (80450683)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 核膜孔複合体 / ヌクレオポリン / 核分化 / 接合 / テトラヒメナ / 細胞核 / 核膜孔 |
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| Research Abstract |
The macronucleus (MAC) and the micronucleus (MIC) of ciliate Tetrahymena thermophila can be distinguished by nucleus-specific homologues of nucleoporin Nup98. In this study, we examined the dynamic behavior of the Nup98s during nuclear differentiation. We observed that MAC-type Nup98 started to assemble on the periphery of the future MACs immediately after the end of the last mitotic division of postzygotic nuclei. This timing was about 30 min earlier than the nuclear transport of factors required for MAC nuclear differentiation. These results suggest that placement of the MAC NPC containing MAC-type Nup98 is the first distinct event triggering further MAC differentiation, probably by leading to MAC-selective nuclear transport.
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate Tetrahymena.2009
Author(s)
Iwamoto, M., Mori, C., Kojidani, T., Bunai, F., Hori, T., Fukagawa, T., Hiraoka, Y., Haraguchi T.
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Journal Title
Current Biology 19
Pages: 843-847
Related Report
Peer Reviewed
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