• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Preferences of synonymous codons and translational control during the chloroplast genome evolution

Research Project

Project/Area Number 21770258
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNagoya City University

Principal Investigator

NAKAMURA Masayuki  Nagoya City University, 大学院・システム自然科学研究科, 研究員 (60322145)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords葉緑体 / 翻訳 / 同義コドン / 進化 / in vitro翻訳系 / 翻訳効率 / コドン使用頻度
Research Abstract

In chloroplasts, the translation efficiencies of synonymous codons are not always correlated with their codon usage. Tobacco chloroplast rps2 mRNA contains many codons having efficient translation activity, whereas rps16 mRNA has a lot of inefficient codons. To elucidate whether the codon preference affect the translation efficiencies of these mRNAs, I analyzed the translation efficiencies of rps2 and rps16 mRNAs using the in vitro translation system from tobacco chloroplasts. I found that (1) 5'UTR of rps16 mRNA has less translation activity, (2) protein coding region of rps16 mRNA was translated about 3-times faster than that of rps2 mRNA, and (3) codon substitutions increased that translation efficiency of protein coding region of rps16 mRNA.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (32 results)

  • [Journal Article] Translation efficiencies of synonymous codons for arginine differ dramatically and are not correlated with codon usage in chloroplasts.2011

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Journal Title

      Gene 472

      Pages: 50-54

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translation efficiencies of synonymous codons for arginine differ dramatically and are not correlated with codon usage in chloroplasts.2011

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Journal Title

      Gene

      Volume: 472 Pages: 50-54

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An organellar maturase associates with multiple group II introns.2010

    • Author(s)
      Zoschke, R., Nakamura, M., Liere, K., Sugiura, M. Borner, T., Schmitz-Linneweber, C.
    • Journal Title

      Proc. Natl. Acad. Sci. USA. 107

      Pages: 3245-3250

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An organellar maturase accosiates with multiple group II introns2010

    • Author(s)
      Zoschke R., Nakamura M., Liere K., Sugiura M., Borner T., Schmitz-Linneweber C.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 107

      Pages: 3245-3250

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 葉緑体ゲノムに存在するrps16遺伝子は機能しているのか?2011

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の核ゲノムにはATG開始コドンを介した新規プロモーターの出現メカニズムがある2011

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] タバコBY-2細胞におけるアミロプラスト分化制御メカニズムの解析2011

    • Author(s)
      本橋典子、江波和彦、小沢友希、中邨真之、田中寛、華岡光正
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] 葉緑体ゲノムに存在するrps16遺伝子は機能しているのか?2011

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物の核ゲノムにはATG開始コドンを介した新規プロモーターの出現メカニズムがある2011

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] タバコBY-2細胞におけるアミロプラスト分化制御メカニズムの解析2011

    • Author(s)
      本橋典子、江波和彦、小沢友希、中邨真之、田中寛、華岡光正
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Translation efficiency of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] 構造遺伝子の挿入によるクリプティックプロモーターの活性化機構の解明2010

    • Author(s)
      松尾充啓、佐藤壮一郎、工藤久幸、中邨真之、山本義治、小保方潤一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] クリプティックプロモーターの活性化メカニズムと転写領域の新生2010

    • Author(s)
      小保方潤一、工藤久幸、松尾充啓、佐藤壮一郎、中邨真之、山本義治、木村宏
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物核に挿入されたコード領域はクロマチンのリモデリングと転写領域の新生を引き起こす2010

    • Author(s)
      工藤久幸、松尾充啓、佐藤壮一郎、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井
    • Related Report
      2010 Final Research Report
  • [Presentation] 葉緑体mRNAの同義コドンと翻訳活性2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井
    • Related Report
      2010 Final Research Report
  • [Presentation] MatK or why keep a chloroplast-encoded splicing factor?2010

    • Author(s)
      Zoschke, R., Neumann, L., Nakamura, M., Liere, K., Sugiura, M., Borner, T., Schmitz-Linneweber, C.
    • Organizer
      XIth International Colloquium on Endocytiobiology and Symbiosis
    • Place of Presentation
      Tromsφ
    • Related Report
      2010 Final Research Report
  • [Presentation] Translation activity of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      XIth International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Tromsφ
    • Related Report
      2010 Final Research Report
  • [Presentation] Chloroplast- and nuclear-encoded rps16 genes in tobacco2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      Gordon Research Conferences ; Mitochondria and Chloroplasts
    • Place of Presentation
      Lucca
    • Related Report
      2010 Final Research Report
  • [Presentation] Comparative analysis of the translation efficiencies between rps2 and rps16 mRNAs in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      FESPB 2010
    • Place of Presentation
      Valencia
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物核に挿入されたコード領域はヌクレオソームと転写単位の再編成を引き起こす2010

    • Author(s)
      工藤久幸、松尾充啓、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] タバコ葉緑体mRNAにおける同義コドンと翻訳効率2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] Comparative analysis of the translation efficiencies between rps2 and rps16 mRNAs in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      FESPB 2010
    • Place of Presentation
      バレンシアカンファレンスセンター(スペイン)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chloroplast- and nuclear-encoded rps16 genes in tobacco2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      Gordon Research Conferences ; Mitochondria and Chloroplasts
    • Place of Presentation
      イル・ショコホテルズ&リゾート(イタリア)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Translation activity of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      XIth International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      トロムソ大学(ノルウェー)
    • Related Report
      2010 Annual Research Report
  • [Presentation] MatK or why keep a chloroplast-encoded splicing factor?2010

    • Author(s)
      Zoschke, R., Neumann, L., Nakamura, M., Liere, K., Sugiura, M., Borner, T. Schmitz-Linneweber, C.
    • Organizer
      XIth International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      トロムソ大学(ノルウェー)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 葉緑体mRNAの同義コドンと翻訳活性2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(愛知県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物核に挿入されたコード領域はクロマチンのリモデリングと転写領域の新生を引き起こす2010

    • Author(s)
      工藤久幸、松尾充啓、佐藤壮一郎、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(愛知県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] クリプティックプロモーターの活性化メカニズムと転写領域の新生2010

    • Author(s)
      小保方潤一、工藤久幸、松尾充啓、佐藤壮一郎、中邨真之、山本義治、木村宏
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Translation efficiency of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 構造遺伝子の挿入によるクリプティックプロモーターの活性化機構の解明2010

    • Author(s)
      松尾充啓、佐藤壮一郎、工藤久幸、中邨真之、山本義治、小保方潤一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] PolIIの転写開始位置の決定に関わるコード領域の役割2009

    • Author(s)
      工藤久幸、山本義治、中邨真之、小保方潤一
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] タバコ葉緑体rps2 mRNAとrps16 mRNAの翻訳効率の比較2009

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] An organellar maturase associates with multiple group II introns2009

    • Author(s)
      Zoschke, R., Nakamura, M., Liere, K., Sugiura, M., Borner, T., Schmitz-Linneweber, C.
    • Organizer
      LEOPOLDINA-SYMPOSIUM
    • Place of Presentation
      Berlin
    • Related Report
      2010 Final Research Report
  • [Presentation] Translation efficiencies of chloroplast mRNAs are modulated by the selection of synonymous codons2009

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      LEOPOLDINA-SYMPOSIUM
    • Place of Presentation
      Berlin
    • Related Report
      2010 Final Research Report
  • [Presentation] Correlation between codon usage and translation efficiencies of synonymous codons in tobacco chloroplasts2009

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      Plant Biology 2009.
    • Place of Presentation
      Honolulu
    • Related Report
      2010 Final Research Report
  • [Presentation] Correlation between codon usage and translation efficiencies of synonymous codons in tobacco chloroplasts2009

    • Author(s)
      Nakamura M., Sugiura M.
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ合衆国)
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi