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2010 Fiscal Year Final Research Report

Preferences of synonymous codons and translational control during the chloroplast genome evolution

Research Project

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Project/Area Number 21770258
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNagoya City University

Principal Investigator

NAKAMURA Masayuki  Nagoya City University, 大学院・システム自然科学研究科, 研究員 (60322145)

Project Period (FY) 2009 – 2010
Keywords葉緑体 / 翻訳 / 同義コドン / 進化 / in vitro翻訳系
Research Abstract

In chloroplasts, the translation efficiencies of synonymous codons are not always correlated with their codon usage. Tobacco chloroplast rps2 mRNA contains many codons having efficient translation activity, whereas rps16 mRNA has a lot of inefficient codons. To elucidate whether the codon preference affect the translation efficiencies of these mRNAs, I analyzed the translation efficiencies of rps2 and rps16 mRNAs using the in vitro translation system from tobacco chloroplasts. I found that (1) 5'UTR of rps16 mRNA has less translation activity, (2) protein coding region of rps16 mRNA was translated about 3-times faster than that of rps2 mRNA, and (3) codon substitutions increased that translation efficiency of protein coding region of rps16 mRNA.

  • Research Products

    (21 results)

All 2011 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (19 results)

  • [Journal Article] Translation efficiencies of synonymous codons for arginine differ dramatically and are not correlated with codon usage in chloroplasts.2011

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Journal Title

      Gene 472

      Pages: 50-54

    • Peer Reviewed
  • [Journal Article] An organellar maturase associates with multiple group II introns.2010

    • Author(s)
      Zoschke, R., Nakamura, M., Liere, K., Sugiura, M. Borner, T., Schmitz-Linneweber, C.
    • Journal Title

      Proc. Natl. Acad. Sci. USA. 107

      Pages: 3245-3250

    • Peer Reviewed
  • [Presentation] 葉緑体ゲノムに存在するrps16遺伝子は機能しているのか?2011

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-20110322
  • [Presentation] 植物の核ゲノムにはATG開始コドンを介した新規プロモーターの出現メカニズムがある2011

    • Author(s)
      佐藤壮一郎、松尾充啓、工藤久幸、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-20110322
  • [Presentation] タバコBY-2細胞におけるアミロプラスト分化制御メカニズムの解析2011

    • Author(s)
      本橋典子、江波和彦、小沢友希、中邨真之、田中寛、華岡光正
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-20110322
  • [Presentation] Translation efficiency of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] 構造遺伝子の挿入によるクリプティックプロモーターの活性化機構の解明2010

    • Author(s)
      松尾充啓、佐藤壮一郎、工藤久幸、中邨真之、山本義治、小保方潤一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] クリプティックプロモーターの活性化メカニズムと転写領域の新生2010

    • Author(s)
      小保方潤一、工藤久幸、松尾充啓、佐藤壮一郎、中邨真之、山本義治、木村宏
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      札幌
    • Year and Date
      20100920-20100922
  • [Presentation] 植物核に挿入されたコード領域はクロマチンのリモデリングと転写領域の新生を引き起こす2010

    • Author(s)
      工藤久幸、松尾充啓、佐藤壮一郎、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井
    • Year and Date
      20100909-20100911
  • [Presentation] 葉緑体mRNAの同義コドンと翻訳活性2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井
    • Year and Date
      20100909-20100911
  • [Presentation] MatK or why keep a chloroplast-encoded splicing factor?2010

    • Author(s)
      Zoschke, R., Neumann, L., Nakamura, M., Liere, K., Sugiura, M., Borner, T., Schmitz-Linneweber, C.
    • Organizer
      XIth International Colloquium on Endocytiobiology and Symbiosis
    • Place of Presentation
      Tromsφ
    • Year and Date
      20100829-20100903
  • [Presentation] Translation activity of rps16 mRNA in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      XIth International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Tromsφ
    • Year and Date
      20100829-20100903
  • [Presentation] Chloroplast- and nuclear-encoded rps16 genes in tobacco2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      Gordon Research Conferences ; Mitochondria and Chloroplasts
    • Place of Presentation
      Lucca
    • Year and Date
      20100710-20100716
  • [Presentation] Comparative analysis of the translation efficiencies between rps2 and rps16 mRNAs in tobacco chloroplasts2010

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      FESPB 2010
    • Place of Presentation
      Valencia
    • Year and Date
      20100604-20100609
  • [Presentation] 植物核に挿入されたコード領域はヌクレオソームと転写単位の再編成を引き起こす2010

    • Author(s)
      工藤久幸、松尾充啓、木村宏、中邨真之、山本義治、小保方潤一
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] タバコ葉緑体mRNAにおける同義コドンと翻訳効率2010

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] PolIIの転写開始位置の決定に関わるコード領域の役割2009

    • Author(s)
      工藤久幸、山本義治、中邨真之、小保方潤一
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] タバコ葉緑体rps2 mRNAとrps16 mRNAの翻訳効率の比較2009

    • Author(s)
      中邨真之、杉浦昌弘
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] An organellar maturase associates with multiple group II introns2009

    • Author(s)
      Zoschke, R., Nakamura, M., Liere, K., Sugiura, M., Borner, T., Schmitz-Linneweber, C.
    • Organizer
      LEOPOLDINA-SYMPOSIUM
    • Place of Presentation
      Berlin
    • Year and Date
      20090920-20090923
  • [Presentation] Translation efficiencies of chloroplast mRNAs are modulated by the selection of synonymous codons2009

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      LEOPOLDINA-SYMPOSIUM
    • Place of Presentation
      Berlin
    • Year and Date
      20090920-20090923
  • [Presentation] Correlation between codon usage and translation efficiencies of synonymous codons in tobacco chloroplasts2009

    • Author(s)
      Nakamura, M., Sugiura, M.
    • Organizer
      Plant Biology 2009.
    • Place of Presentation
      Honolulu
    • Year and Date
      20090718-20090722

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Published: 2012-02-13   Modified: 2016-04-21  

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