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Analysis of the relationship between herbicide resistance mechanism and biophoton generation

Research Project

Project/Area Number 21780018
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Crop science/Weed science
Research InstitutionShizuoka Prefectural Research Institute of Agriculture and Forestry

Principal Investigator

NUKUI Hideki  Shizuoka Prefectural Research Institute of Agriculture and Forestry, 花き科, 主任研究員 (10426463)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords除草剤抵抗性 / バイオフォトン
Research Abstract

The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] Involvement of cytochrome P450 in bensulfronmethyl responsive photon emission from rice cells2011

    • Author(s)
      Nukui, H., Iyozumi, H., Kato, K., Kageyama, C.
    • Organizer
      51^<st> Annual Meeting of the Weed Science Society of America
    • Place of Presentation
      Hilton Portland & Executive Tower, Portland, Oregon
    • Related Report
      2010 Final Research Report
  • [Presentation] Involvement of cytochrome P450 in bensulfronmethyl responsive photon emission from rice cells2011

    • Author(s)
      Nukui, H.
    • Organizer
      Weed Science Society of America
    • Place of Presentation
      Hilton Portland & Executive Tower, Portland, Oregon
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネ培養細胞の除草剤応答発光2010

    • Author(s)
      貫井秀樹
    • Organizer
      日本雑草学会
    • Place of Presentation
      AOSSA福井市地域交流プラザ・福井県県民ホール(福井県)
    • Year and Date
      2010-04-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネ培養細胞の除草剤応答発光2010

    • Author(s)
      貫井秀樹、伊代住浩幸、加藤公彦
    • Organizer
      日本雑草学会
    • Place of Presentation
      AOSSA福井市地域交流プラザ・福井県県民ホール
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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