Comprehensive screening of novel microbial enzymes toward on-demand production of hydroxy amino acids
Project/Area Number |
21780070
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
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Research Institution | Kyoto University |
Principal Investigator |
HIBI Makoto Kyoto University, 大学院・農学研究科, 助教 (30432347)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | 水酸化アミノ酸 / 微生物スクリーニング / ジオキシゲナーゼ / 酵素触媒 / 微生物 / 水酸化酵素 / 基質特異性 / スクリーニング / キラルアルコール / N置換アミノ酸 / アミノ酸 / 水酸化 / 酵素 |
Research Abstract |
Hydroxy amino acids are promising compounds for the use to drugs and foods, because some of them possess useful physiological activities. Then efficient production method for hydroxy amino acids is desired. In this research, four dioxygenases obtained from microorganisms were found to catalyze hydroxylation of amino acids, and their substrate specificities and chemical structure of the products were determined. The hydroxylating activities of these dioxygenases were novel types of reactions, and all of their reaction was highly stereospecific. Thus these dioxygenases were useful for the biocatalysts for the production of chiral hydroxy amino acids.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] A novel L-isoleucine metabolism i n Bacillus thuringiensis generating (2S, 3R,4S)-4-hydroxyisoleucine2011
Author(s)
Ogawa J, Kodera T, Smirnov SV, Hibi M, Samsonova NN, Koyama R, Yamanaka H, Mano J, Kawashima T, Yokozeki K, Shimiz u S.
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Journal Title
a potential insulinotropic and anti-obesity amino acid, Applied Microbiology and Biotechn olog 89
Pages: 1929-1938
Related Report
Peer Reviewed
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[Journal Article] Metabolic engineering of Escherich ia coli to produce (2S,3R,4S)-4-hydroxy isoleucine2010
Author(s)
Smirnov SV, Kodera T , Samsonova NN , Kotlyarova VA, Rushkevich NY, Kivero AD, Sokolov PM, Hibi M, Ogawa J, Shimizu S.
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Journal Title
Applied Microbiology and Bi otechnology 88
Pages: 719-726
Related Report
Peer Reviewed
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[Journal Article] Metabolic engineering of Escherichia coli to produce(2S,3R,4S)-4-hydroxyisoleucine2010
Author(s)
Smirnov SV, Kodera T, Samsonova NN, Kotlyarova VA, Rushkevich NY, Kivero AD, Sokolov PM, Hibi M, Ogawa J, Shimizu S.
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Journal Title
Applied Microbiology and Biotechnology
Volume: 88
Pages: 719-726
Related Report
Peer Reviewed
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[Journal Article] A novel L-isoleucine metabolism in Bacillus thuringiensis generating (2S,3R,4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid2010
Author(s)
Ogawa J, Kodera T, Smirnov SV, Hibi M, Samsonova NN, Koyama R, Yamanaka H, Mano J, Kawashima T, Yokozeki K, Shimizu S.
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Journal Title
Applied Microbiology and Biotechnology
Volume: 89
Pages: 1929-1938
Related Report
Peer Reviewed
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[Journal Article] A novel l-isoleuc ine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine2009
Author(s)
Kodera T, Smirnov SV, Samsonova NN, Kozlov YI, Koyama R, Hibi M, Ogawa J, Y okozeki K, Shimizu S
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Journal Title
Biochemical and Biophysical Research Communications. 390
Pages: 506-510
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] Method for producing hydroxylated amino acids2011
Inventor(s)
Smirnov, S.V., Samsonova, N.N., Kotliarova, V.A., Rushkevich, N.Y., Fedorina, E.A., Sokolov, P.M., Kolokolova, A.V., Ogawa, J., Hibi, M., Shimizu, S., Imabayashi, Y., Suzuki, S., Sugiyama, M.
Industrial Property Rights Holder
Ajinomoto Co., Inc.
Industrial Property Number
PTC特許,PT2011021717
Filing Date
2011-02-24
Related Report
Overseas
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[Patent(Industrial Property Rights)] METHOD FOR PRODUCING HYDROXYLATED AMINO ACIDS2011
Inventor(s)
SMIRNOV, S.V.SAMSONOVA, N.N., KOTLIAROVA, V.A., RUSHKEVICH, N.Y., FEDORINA, E.A., SOKOLOV, P.M., KOLOKOLOVA, A.V., OGAWA, J., HIBI, M., SHIMIZU, S., IMABA YASHI, Y., SUZUKI S., SUGIYAMA, M.
Industrial Property Rights Holder
AJINO MOTO CO., INC.
Industrial Property Number
WO/2011/021717
Filing Date
2011-02-24
Related Report
Overseas
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[Patent(Industrial Property Rights)] METHOD FOR PRODUCING HYDROXYLATED AMINO ACIDS2010
Inventor(s)
SMIRNOV, S.V.SAMSONOVA, N.N., KOTLIAROVA, V.A., RUSHKEVICH, N.Y., FEDORINA, E.A., SOKOLOV, P.M., KOLOKOLOVA, A.V., OGAWA, J., HIBI, M., SHIMIZU, S., IMABA YASHI, Y., SUZUKI S., SUGIYAMA, M.
Industrial Property Rights Holder
AJINO MOTO CO., INC.
Industrial Property Number
WO/2011/021717
Acquisition Date
2010-08-18
Related Report
Overseas