Regulation of blood pressure by secreted adipocyte-derived leucine aminopeptidase

• Project/Area Number: 21780103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Teikyo Heisei University (2010); The Institute of Physical and Chemical Research (2009)
• Principal Investigator: GOTO Yoshikuni Teikyo Heisei University, 薬学部, 助教 (90455345)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: アミノペプチダーゼ / 血圧調節 / マクロファージ / 一酸化窒素 / 分泌 / インターフェロン / リポ多糖 / LPS / 貪食

Research Abstract

Adipocyte-derived leucine amnopeptidase (A-LAP) may play role in the blood pressure regulation. In this study, it is revealed that ER-retained A-LAP is secreted from macrophages in response to activation by treatment with lipopolysaccharide and interferon-γ, and supply of arginine for nitric oxide synthesis from peptide to cells. These results suggest that secretion of A-LAP is important for hypertensive action.

Research Products

• [Journal Article] Secretion of endoplasmic reticulum aminopeptidase 1 is involved in the activation of macrophages induced by lipopolysaccharide and interferon-γ. (2011)
  • Author(s): Goto Y, Ogawa K, Hattori A, Tsujimoto M.
  • Journal Title: J Biol Chem. in press
  • Peer Reviewed
• [Journal Article] Coding single nucleotide polymorphisms of endoplasmic reticulum aminopeptidase 1 can affect antigenic peptide generation in vitro by influencing basic enzymatic properties of the enzyme. (2011)
  • Author(s): Evnouchidou I, Kamal RP, Seregin SS, Goto Y, Tsujimoto M, Hattori A, Voulgari PV, Drosos AA, Amalfitano A, York IA, Stratikos E.
  • Journal Title: J Immunol. 186 Pages: 1909-1913
  • Peer Reviewed
• [Journal Article] Involvement of glutamine-238 in the substrate specificity of human laeverin/aminopeptidase Q. (2011)
  • Author(s): Goto Y, Yoshioka R, Arisaka N, Hattori A, Tsujimoto M.
  • Journal Title: Biol Pharm Bull. 34 Pages: 24-27
  • NAID: 130000402257
  • Peer Reviewed
• [Journal Article] Coding Single Nucleotide Polymorphisms of Endoplasmic Reticulum Aminopeptidase 1 Can Affect Antigenic Peptide Generation In Vitro by Influencing Basic Enzymatic Properties of the Enzyme (2011)
  • Author(s): Irini Evnouchidou
  • Journal Title: Journal of Immunology Volume: 186 Pages: 1909-1913
  • Peer Reviewed
• [Journal Article] Involvement of glutamine-238 in the substrate specificity of human laeverin/aminopeptidase Q (2011)
  • Author(s): Yoshikuni Goto
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 34 Pages: 24-27
  • NAID: 130000402257
  • Peer Reviewed
• [Journal Article] Secretion of endoplasmic reticulum aminopeptidase 1 is involved in the activation of macrophages induced by lipopolysaccharide and interferon-γ
  • Author(s): Yoshikuni Goto
  • Journal Title: Journal of Biological Chemistry Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Phagocytosis of macrophages is facilitated by secreted ER-aminopeptidase 1 (2010)
  • Author(s): Yoshikuni Goto
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-10
• [Presentation] ERAP-1/A-LAP is secreted form macrophages treated with IFN-γ and LPS (2009)
  • Author(s): Yoshikuni Goto
  • Organizer: 6th General meeting of International proteolysis society 2009 conference
  • Place of Presentation: Surfers Paradise QD Australia
  • Year and Date: 2009-10-28
• [Presentation] ERAP-1/A-LAP is secreted form macrophages treated with IFN-γ and LPS (2009)
  • Author(s): 後藤芳邦
  • Organizer: 6^<th> General meeting of International proteolysis society 2009 conference
  • Place of Presentation: Surfers Paradise QD Australia
  • Year and Date: 2009-10-28
• [Presentation] マクロファージにおけるカルシウムを介した小胞体アミノペプチダーゼ-1の分泌 (2009)
  • Author(s): 後藤芳邦
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2009-10-23
• [Presentation] マクロファージにおけるカルシウムを介した小胞体アミノペプチダーゼ-1の分泌 (2009)
  • Author(s): 後藤芳邦
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 兵庫県 神戸市
  • Year and Date: 2009-10-23
• [Presentation] マクロファージにおける小胞体アミノペプチダーゼ-1の分泌 (2009)
  • Author(s): 後藤芳邦
  • Organizer: 第14回病態プロテアーゼ学会
  • Place of Presentation: 千里ライフサイエンスセンター(吹田)
  • Year and Date: 2009-08-21
• [Presentation] マクロファージにおける小胞体アミノペプチダーゼ-1の分泌 (2009)
  • Author(s): 後藤芳邦
  • Organizer: 第14回病態プロテアーゼ学会
  • Place of Presentation: 大阪府 吹田市
  • Year and Date: 2009-08-21