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Development of eastern blotting technique for high-throughput analysis of kampo medicines

Research Project

Project/Area Number 21790030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemical pharmacy
Research InstitutionNagasaki International University

Principal Investigator

MORINAGA Osamu  Nagasaki International University, 薬学部, 講師 (60465771)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsイースタンブロット / モノクローナル抗体 / 漢方薬 / 配糖体 / 免疫染色 / イムノアッセイ / 品質評価
Research Abstract

We demonstrated the high-throughput analysis for natural glycosides in kampo medicines using monoclonal antibodies (MAbs) and eastern blotting technique. Then, rapid, simple, high-sensitive and specific detection system was developed for GC and BI by eastern blotting technique using dot blot and chemiluminescent methods. This methods was useful as the high-throughput analysis for the determination of active compounds in kampo medicines as well as HPLC and ELISA systems.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (10 results) Remarks (3 results)

  • [Journal Article] Development of new staining technology "eastern blotting" using monoclonal antibody2011

    • Author(s)
      Osamu Morinaga、Yukihiro Shoyama
    • Journal Title

      Curr Drug Discov Technol 8巻

      Pages: 42-50

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of new staining technology "eastern blotting" using monoclonal antibody2011

    • Author(s)
      O.Morinaga, et al.
    • Journal Title

      Curr Drug Discov Technol.

      Volume: 8 Pages: 42-50

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts2010

    • Author(s)
      Osamu Morinaga、Takuhiro Uto、Chun-Su Yuan、Hiroyuki Tanaka、Yukihiro Shoyama
    • Journal Title

      Fitoterapia 81巻

      Pages: 284-288

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 東西南北ブロット法:東は終着駅?2010

    • Author(s)
      森永紀
    • Journal Title

      生物工学会誌

      Volume: 88 Pages: 411-411

    • NAID

      110007701246

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of a new eastern blotting technique for the analysis of ginse noside Re in American ginseng berry pulp extracts2010

    • Author(s)
      O.Morinaga, et al.
    • Journal Title

      Fitoterapia

      Volume: 81 Pages: 284-288

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts2010

    • Author(s)
      Osamu MORINAGA
    • Journal Title

      FITOTERAPIA VOL.81

      Pages: 284-288

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イースタンブロットを用いた漢方薬のハイスループット分析法2011

    • Author(s)
      森永紀、藤井俊輔、Halermat Suktap、迎涼、宇都拓洋、田中宏幸、正山征洋
    • Organizer
      日本薬学会、第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-31
    • Related Report
      2010 Final Research Report
  • [Presentation] イースタンブロットを用いた漢方薬のハイスループット分析法2011

    • Author(s)
      森永紀
    • Organizer
      日本薬学会 第131年会
    • Place of Presentation
      静岡県立大学(静岡市)
    • Year and Date
      2011-03-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイカリンに対するイースタンブロット法の開発と定性・定量分析への応用2010

    • Author(s)
      迎涼、宇都拓洋、森永紀、正山征洋
    • Organizer
      日本薬学会九州支部大会、第27回
    • Place of Presentation
      長崎
    • Year and Date
      2010-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] バイカリンに対するイースタンブロット法の開発と定性・定量分析への応用2010

    • Author(s)
      森永紀
    • Organizer
      第27回 日本薬学会九州支部大会
    • Place of Presentation
      長崎大学(長崎市)
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Eastern Blottingを基盤としたグリチルリチンの新規定量分析法について2010

    • Author(s)
      森永紀
    • Organizer
      第27回 日本薬学会九州支部大会
    • Place of Presentation
      長崎大学(長崎市)
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] グリチルリチン特異的抗体と化学発光法を用いたグリチルリチンの新規定量分析法の開発2010

    • Author(s)
      藤井俊輔、森永紀、正山征洋
    • Organizer
      日本生薬学会、第57年会
    • Place of Presentation
      徳島
    • Year and Date
      2010-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] グリチルリチン特異的抗体と化学発光法を用いたグリチルリチンの新規定量分析法の開発2010

    • Author(s)
      森永紀
    • Organizer
      日本生薬学会 第57年会
    • Place of Presentation
      徳島文理大学(徳島市)
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Baicalin に対する New Eastern Blotting の確立とその応用2010

    • Author(s)
      森永紀
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      桃太郎アリーナ(岡山市)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] モノクローナル抗体による甘草の品質及び活性評価解析2009

    • Author(s)
      森永紀
    • Organizer
      第38回 生薬分析シンポジウム
    • Place of Presentation
      薬業年金会館(大阪市)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of Paeoniflorin and Albiflorin in Crude Drugs and Traditional Chinese Medicines by Enzyme-linked Immunosorbent Assay, New Eastern Blotting Technique and Immunochromatographic Strip Test Using a Monoclonal Antibody2009

    • Author(s)
      森永紀
    • Organizer
      2009'Fukuoka International Symposium for Pharmaceutical Sciences in JSPS Asian Core Program
    • Place of Presentation
      アクロス福岡(福岡市)
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.niu.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.niu.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.niu.ac.jp/~pharml/lab/pharmacognosy/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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