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Study on functions of biological clock during chondrogenesis using bioluminescence imaging

Research Project

Project/Area Number 21790195
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionHokkaido University

Principal Investigator

KWON Hyuckjoon  Hokkaido University, 大学院・医学研究科, 特任助教 (90447045)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords軟骨分化 / 発光イメージング / 生物時計 / 代謝リズム / 軟骨形成 / 分子時計 / ATPのウルトラディアンリズム / 生物発光イメージング / ATP wave / 細胞間のコミュニケーション- / 発光酵素ルシフェラーゼ / ウルトラディアンリズム / リアルタイムイメージング
Research Abstract

We have constructed bioluminescence imaging system for monitoring dual reporter expression at the single-cell level. Using this system, we succeeded in observing that bmal1 gene expression oscillates in anti-phase with per2 gene expression. In addition, bioluminescence imaging has showed that metabolic oscillations to exhibit coupled oscillations of ATP and oxygen are induced during chondrogenesis. We found that the metabolic oscillations are involved in prechondrogenic condensation, not in chondrogenic differentiation.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] In vivo imaging of particle-induced inflammation and osteolysis in the calvariae of NFkB/Luciferase transgenic mice.2011

    • Author(s)
      Takahashi K, Onodera S, Tohyama H, Kwon, HJ, Honma K, Yasuda K.
    • Journal Title

      Journal of Biomedicine and Biotechnology 2011 2011

      Pages: 727063-727063

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Vivo Imaging of Particle-induced inflammation and osteolysis in the calvariae of NFkB/Luciferase transgenic mice.2011

    • Author(s)
      Takahashi K, Onodera S, Tohyama H, Kwon HJ, Honma K, Yasuda K.
    • Journal Title

      Journal of Biomedicine and Biotechnology

      Volume: 2011 Pages: 1-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioluminescence imaging of dual gene expression at the single-cell level.2010

    • Author(s)
      Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
    • Journal Title

      Biotechniques 48

      Pages: 460-462

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities.2010

    • Author(s)
      Kwon H J, Yasuda K, Ohmiya Y, Honma K, Chen YM, Gong JP.
    • Journal Title

      Acta Biomaterialia 6

      Pages: 494-501

    • NAID

      120003702860

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bioluminescence imaging of dual gene expression at the single-cell level.2010

    • Author(s)
      Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y
    • Journal Title

      BioTechniques

      Volume: 48 Pages: 460-462

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities2010

    • Author(s)
      権赫準
    • Journal Title

      Acta Biomaterialia 6

      Pages: 494-501

    • NAID

      120003702860

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced in situ formation of various F-actin assemblies with photoresponsive polycation.2009

    • Author(s)
      Misu M, Furukawa H, Kwon H J, Shikinaka K, Kakugo A, Sato T, Osada Y, Gong, JP.
    • Journal Title

      Journal of Biomedical Materials Research : Part A 89

      Pages: 424-431

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo 4 bioluminescence imaging of bone marrow-derived cells in brain inflammation.2009

    • Author(s)
      Akimoto H, Kwon HJ, Ozaki M, Yasuda K, Honma K, Ohmiya, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 380

      Pages: 844-849

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect.2009

    • Author(s)
      Yasuda K, Kitamura N, Gong JP, Arakaki K, Kwon HJ, Onodera S, Chen YM, Kurokawa T, Kanaya F, Ohmiya Y, Osada Y.
    • Journal Title

      Macromolecular Bioscience 9

      Pages: 307-316

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Double-Network Hydrogel Induces Spontaneous Articular Cartilage Regeneration In Vivo in a Large Osteochondral Defect2009

    • Author(s)
      権赫準
    • Journal Title

      Macromol.Biosci. 9

      Pages: 307-316

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo bioluminescence imaging of bone marrow-derived cells in brain inflammation2009

    • Author(s)
      権赫準
    • Journal Title

      Biochem.Biophys.Res.Commun. 380

      Pages: 844-849

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced in situ Formation of Various F-Actin Assemblies with Photoresponsive Polycation2009

    • Author(s)
      権赫準
    • Journal Title

      Journal of Biomedical Materials Research : Part A 89

      Pages: 424-431

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level.2010

    • Author(s)
      権赫準
    • Organizer
      第33回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level.2010

    • Author(s)
      Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
    • Organizer
      The 32^<nd> annual meeting of the molecular biology society of Japan.
    • Place of Presentation
      Kobe Port Island, Kobe, Japan.
    • Related Report
      2010 Final Research Report
  • [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level The 8th International Symposium for Future Drug Discovery and Medical Care2010

    • Author(s)
      Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
    • Organizer
      [Molecular Imaging for Treatment Monitoring]
    • Place of Presentation
      Sapporo, Japan.
    • Related Report
      2010 Final Research Report
  • [Presentation] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities2009

    • Author(s)
      権赫準
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities.2009

    • Author(s)
      Kwon HJ, Yasuda K, Ohmiya Y, Honma K, Chen YM, Gong JP.
    • Organizer
      The 32^<nd> annual meeting of the molecular biology society of Japan.
    • Place of Presentation
      Pacific Yokohama, Yokohama, Japan.
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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