Study on functions of biological clock during chondrogenesis using bioluminescence imaging
Project/Area Number |
21790195
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General physiology
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Research Institution | Hokkaido University |
Principal Investigator |
KWON Hyuckjoon Hokkaido University, 大学院・医学研究科, 特任助教 (90447045)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 軟骨分化 / 発光イメージング / 生物時計 / 代謝リズム / 軟骨形成 / 分子時計 / ATPのウルトラディアンリズム / 生物発光イメージング / ATP wave / 細胞間のコミュニケーション- / 発光酵素ルシフェラーゼ / ウルトラディアンリズム / リアルタイムイメージング |
Research Abstract |
We have constructed bioluminescence imaging system for monitoring dual reporter expression at the single-cell level. Using this system, we succeeded in observing that bmal1 gene expression oscillates in anti-phase with per2 gene expression. In addition, bioluminescence imaging has showed that metabolic oscillations to exhibit coupled oscillations of ATP and oxygen are induced during chondrogenesis. We found that the metabolic oscillations are involved in prechondrogenic condensation, not in chondrogenic differentiation.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] A Novel Double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect.2009
Author(s)
Yasuda K, Kitamura N, Gong JP, Arakaki K, Kwon HJ, Onodera S, Chen YM, Kurokawa T, Kanaya F, Ohmiya Y, Osada Y.
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Journal Title
Macromolecular Bioscience 9
Pages: 307-316
Related Report
Peer Reviewed
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