Study on functions of biological clock during chondrogenesis using bioluminescence imaging

• Project/Area Number: 21790195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Hokkaido University
• Principal Investigator: KWON Hyuckjoon Hokkaido University, 大学院・医学研究科, 特任助教 (90447045)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 軟骨分化 / 発光イメージング / 生物時計 / 代謝リズム / 軟骨形成 / 分子時計 / ATPのウルトラディアンリズム / 生物発光イメージング / ATP wave / 細胞間のコミュニケーション- / 発光酵素ルシフェラーゼ / ウルトラディアンリズム / リアルタイムイメージング

Research Abstract

We have constructed bioluminescence imaging system for monitoring dual reporter expression at the single-cell level. Using this system, we succeeded in observing that bmal1 gene expression oscillates in anti-phase with per2 gene expression. In addition, bioluminescence imaging has showed that metabolic oscillations to exhibit coupled oscillations of ATP and oxygen are induced during chondrogenesis. We found that the metabolic oscillations are involved in prechondrogenic condensation, not in chondrogenic differentiation.

Research Products

• [Journal Article] In vivo imaging of particle-induced inflammation and osteolysis in the calvariae of NFkB/Luciferase transgenic mice. (2011)
  • Author(s): Takahashi K, Onodera S, Tohyama H, Kwon, HJ, Honma K, Yasuda K.
  • Journal Title: Journal of Biomedicine and Biotechnology 2011 2011 Pages: 727063-727063
  • Peer Reviewed
• [Journal Article] In Vivo Imaging of Particle-induced inflammation and osteolysis in the calvariae of NFkB/Luciferase transgenic mice. (2011)
  • Author(s): Takahashi K, Onodera S, Tohyama H, Kwon HJ, Honma K, Yasuda K.
  • Journal Title: Journal of Biomedicine and Biotechnology Volume: 2011 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of dual gene expression at the single-cell level. (2010)
  • Author(s): Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
  • Journal Title: Biotechniques 48 Pages: 460-462
  • Peer Reviewed
• [Journal Article] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities. (2010)
  • Author(s): Kwon H J, Yasuda K, Ohmiya Y, Honma K, Chen YM, Gong JP.
  • Journal Title: Acta Biomaterialia 6 Pages: 494-501
  • NAID: 120003702860
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of dual gene expression at the single-cell level. (2010)
  • Author(s): Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y
  • Journal Title: BioTechniques Volume: 48 Pages: 460-462
  • Peer Reviewed
• [Journal Article] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities (2010)
  • Author(s): 権赫準
  • Journal Title: Acta Biomaterialia 6 Pages: 494-501
  • NAID: 120003702860
  • Peer Reviewed
• [Journal Article] Photoinduced in situ formation of various F-actin assemblies with photoresponsive polycation. (2009)
  • Author(s): Misu M, Furukawa H, Kwon H J, Shikinaka K, Kakugo A, Sato T, Osada Y, Gong, JP.
  • Journal Title: Journal of Biomedical Materials Research : Part A 89 Pages: 424-431
  • Peer Reviewed
• [Journal Article] In vivo 4 bioluminescence imaging of bone marrow-derived cells in brain inflammation. (2009)
  • Author(s): Akimoto H, Kwon HJ, Ozaki M, Yasuda K, Honma K, Ohmiya, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 380 Pages: 844-849
  • Peer Reviewed
• [Journal Article] A Novel Double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect. (2009)
  • Author(s): Yasuda K, Kitamura N, Gong JP, Arakaki K, Kwon HJ, Onodera S, Chen YM, Kurokawa T, Kanaya F, Ohmiya Y, Osada Y.
  • Journal Title: Macromolecular Bioscience 9 Pages: 307-316
  • Peer Reviewed
• [Journal Article] A Novel Double-Network Hydrogel Induces Spontaneous Articular Cartilage Regeneration In Vivo in a Large Osteochondral Defect (2009)
  • Author(s): 権赫準
  • Journal Title: Macromol.Biosci. 9 Pages: 307-316
  • Peer Reviewed
• [Journal Article] In vivo bioluminescence imaging of bone marrow-derived cells in brain inflammation (2009)
  • Author(s): 権赫準
  • Journal Title: Biochem.Biophys.Res.Commun. 380 Pages: 844-849
  • Peer Reviewed
• [Journal Article] Photoinduced in situ Formation of Various F-Actin Assemblies with Photoresponsive Polycation (2009)
  • Author(s): 権赫準
  • Journal Title: Journal of Biomedical Materials Research : Part A 89 Pages: 424-431
  • Peer Reviewed
• [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level. (2010)
  • Author(s): 権赫準
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-09
• [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level. (2010)
  • Author(s): Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
  • Organizer: The 32^<nd> annual meeting of the molecular biology society of Japan.
  • Place of Presentation: Kobe Port Island, Kobe, Japan.
• [Presentation] Bioluminescence imaging of dual gene expression at the single-cell level The 8th International Symposium for Future Drug Discovery and Medical Care (2010)
  • Author(s): Kwon HJ, Enomoto T, Shimogawara M, Yasuda K, Nakajima Y, Ohmiya Y.
  • Organizer: [Molecular Imaging for Treatment Monitoring]
  • Place of Presentation: Sapporo, Japan.
• [Presentation] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities (2009)
  • Author(s): 権赫準
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities. (2009)
  • Author(s): Kwon HJ, Yasuda K, Ohmiya Y, Honma K, Chen YM, Gong JP.
  • Organizer: The 32^<nd> annual meeting of the molecular biology society of Japan.
  • Place of Presentation: Pacific Yokohama, Yokohama, Japan.
• [Remarks] ホームページ等