| Project/Area Number |
21790418
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
TOSHIMA Hirono Osaka University, 微生物病研究所, 特任研究員 (10400532)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細菌毒素 / ウエルシュ菌 / クローディン |
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| Research Abstract |
Clostridium perfringens enterotoxin (CPE), a causative agent of food poisoning, is a pore-forming toxin. Claudin, a component of tight junctions, is a tetratransmembrane protein and constitutes a large family of more than 20 members, not all of which serve as the receptor for CPE. The mechanism by which the toxin distinguishes the sensitive claudins is unknown. In this study, we localized the region of claudin responsible for interaction with CPE to the C-terminal 12 amino acids of the second extracellular loop and found that an electrostatic attraction between the basic claudin region and the acidic CPE cleft is involved in their interaction. Furthermore, we present the structure of CPE determined by X-ray crystallography at 2.0 Å. These structural features imply that CPE is a β pore-forming toxin.
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